Five geranylgeranyl diphosphate synthases expressed in different organs are localized into three subcellular compartments in Arabidopsis.

abstract：:Although researchers have established that DNA methylation and active demethylation are dynamically regulated in plant cells, the molecular mechanism for the regulation of active DNA demethylation is not well understood. By using an Arabidopsis (Arabidopsis thaliana) line expressing the Promoter RESPONSIVE TO DEHYDRAT...

journal_title：Plant physiology

authors： Wang C,Dong X,Jin D,Zhao Y,Xie S,Li X,He X,Lang Z,Lai J,Zhu JK,Gong Z

更新日期：2015-03-01 00:00:00

abstract：:A method for the preparation of developmentally staged microspores and young pollen from maize (Zea mays) has been devised. The preparations are of sufficient purity and quantity for biochemical analysis, including the analysis of steady-state protein and RNA populations associated with each stage. A major transition ...

journal_title：Plant physiology

authors： Bedinger PA,Edgerton MD

更新日期：1990-02-01 00:00:00

abstract：:Net sucrose efflux from discs of fully expanded leaves of soybean (Glycine max [L.] Merr.) plants was studied to characterize sucrose efflux into the apoplast. Net sucrose efflux had a Q(10) of 2.3, was linear for at least 3.5 hours, and was selective for sucrose over glucose. Sulfhydryl group inhibitors reduced sucro...

journal_title：Plant physiology

authors： Secor J

更新日期：1987-01-01 00:00:00

abstract：:Nucleotide metabolism in potato (Solanum tuberosum) mitochondria was studied using 31P-nuclear magnetic resonance spectroscopy and the O2 electrode. Immediately following the addition of ADP, ATP synthesis exceeded the rate of oxidative phosphorylation, fueled by succinate oxidation, due to mitochondrial adenylate kin...

journal_title：Plant physiology

authors： Roberts J,Aubert S,Gout E,Bligny R,Douce R

更新日期：1997-01-01 00:00:00

abstract：:DNA and histone methylation coregulate heterochromatin formation and gene silencing in animals and plants. To identify factors involved in maintaining gene silencing, we conducted a forward genetic screen for mutants that release the silenced transgene Pro35S::NEOMYCIN PHOSPHOTRANSFERASE II in the transgenic Arabidops...

journal_title：Plant physiology

authors： Meng J,Wang L,Wang J,Zhao X,Cheng J,Yu W,Jin D,Li Q,Gong Z

更新日期：2018-06-01 00:00:00

abstract：:Polyamines are small ubiquitous molecules that have been involved in nearly all developmental processes, including the stress response. Nevertheless, no direct evidence of a role of polyamines in the wound response has been described. We have studied the expression of genes involved in polyamine biosynthesis in respon...

journal_title：Plant physiology

authors： Perez-Amador MA,Leon J,Green PJ,Carbonell J

更新日期：2002-11-01 00:00:00

abstract：:Two major proteins (the 7S and 11S globulins) of soybean (Glycine max) were simultaneously isolated by a simple method based on their different solubilities in dilute tris (hydroxymethyl) aminomethane buffers. The purified 7S globulins, which represented essentially the entire 7S soybean protein fraction capable of di...

journal_title：Plant physiology

authors： Thanh VH,Okubo K,Shibasaki K

更新日期：1975-07-01 00:00:00

abstract：:The pigment content of dark-grown primary needles of Pinus jeffreyi L. and Pinus sylvestris L. was determined by high-performance liquid chromatography. The state of protochlorophyllide a and of chlorophylls during dark growth were analyzed by in situ 77 K fluorescence spectroscopy. Both measurements unambiguously dem...

journal_title：Plant physiology

authors： Schoefs B,Franck F

更新日期：1998-12-01 00:00:00

abstract：:Soybean plants (Glycine max var. Ransom) were grown at light intensities of 850 and 250 mueinsteins m(-2) sec(-1) of photosynthetically active radiation. A group of plants was shifted from each environment into the other environment 24 hours before the beginning of the experiment. Net photosynthetic rates and stomatal...

journal_title：Plant physiology

authors： Bunce JA,Patterson DT,Peet MM

更新日期：1977-08-01 00:00:00

abstract：:Respiratory control ratios between 2.0 and 9.0 were obtained by comparison of the respiratory rates of cabbage mitochondria in the presence and in the absence of individual components of the system used to provide ADP and by comparing the rates before and after exhaustion of added ADP. These results indicate that resp...

journal_title：Plant physiology

authors： Lotlikar PD,Remmert LF

更新日期：1968-03-01 00:00:00

abstract：:Photorespiration rates in tissue cultures of a C(4) plant, Portulaca oleracea, were compared to those in tissue cultures of a C(3) plant, Streptanthus tortuosus. The C(4) plant tissue cultures have one-half to one-third the photorespiration rate of the C(3) plant tissue cultures and respond to varying O(2) concentrati...

journal_title：Plant physiology

authors： Kennedy RA

更新日期：1976-10-01 00:00:00

abstract：:Crude aqueous extracts from Arabidopsis leaves were subjected to chromatographic separations, after which the different fractions were monitored for antimicrobial activity using the fungus Neurospora crassa as a test organism. Two major fractions were obtained that appeared to have the same abundance in leaves from un...

journal_title：Plant physiology

authors： Tierens KF,Thomma BP,Brouwer M,Schmidt J,Kistner K,Porzel A,Mauch-Mani B,Cammue BP,Broekaert WF

更新日期：2001-04-01 00:00:00

abstract：:The incorporation of [(14)C]phenylalanine and [1,6-(14)C]shikimic acid into tyrosine was investigated in the callus of Portulaca grandiflora, var. JR (L.). By inhibiting phenylalanine with 1-alpha-aminooxy-beta-phenyl-propionic acid and tyrosinase with 1-cysteine-HCl and hydrazine-hydrate, the possible synthesis of ty...

journal_title：Plant physiology

authors： Endress R

更新日期：1981-08-01 00:00:00

abstract：:Hydroxy-l-proline-induced inhibition of elongation of Avena coleoptile segments was measured in water and in indole-3-acetic acid. This inhibition was completely reversed by l-proline.Time-sequence experiments revealed that some time had to elapse, or some elongation had to occur, prior to the onset of hydroxyproline ...

journal_title：Plant physiology

authors： Norris WE

更新日期：1967-04-01 00:00:00

abstract：:Microbe-associated molecular pattern-triggered immunity (MTI) is an important component of the plant innate immunity response to invading pathogens. However, most of our knowledge of MTI comes from studies of model systems with relatively little work done with crop plants. In this work, we report on variation in both ...

journal_title：Plant physiology

authors： Valdés-López O,Thibivilliers S,Qiu J,Xu WW,Nguyen TH,Libault M,Le BH,Goldberg RB,Hill CB,Hartman GL,Diers B,Stacey G

更新日期：2011-12-01 00:00:00

abstract：:This study was performed to test if alternative carbon sources besides recently photosynthetically fixed CO2 are used for isoprene formation in the leaves of young poplar (Populus x canescens) trees. In a 13CO2 atmosphere under steady state conditions, only about 75% of isoprene became 13C labeled within minutes. A co...

journal_title：Plant physiology

authors： Schnitzler JP,Graus M,Kreuzwieser J,Heizmann U,Rennenberg H,Wisthaler A,Hansel A

更新日期：2004-05-01 00:00:00

abstract：:The nitrite-reducing activity of the normal susceptible biotype of lambsquarters (Chenopodium album L.) was strongly inhibited by atrazine in the assay medium, both in the case of the in vivo assays of leaf discs in light, and in vitro photoreduction assays of crude extracts. In vitro assays of crude extracts with met...

journal_title：Plant physiology

authors： Lawrence JM,Foster RJ,Herrick HE

更新日期：1980-05-01 00:00:00

abstract：:Our goal was to create a DNA chip that is as easy, convenient, and inexpensive as an agarose gel. For a first-generation solution, we describe a low-cost, easy-to-use de novo synthesis oligonucleotide microarray technology that draws on the inherent flexibility of the maskless array synthesizer for in situ synthesis o...

journal_title：Plant physiology

authors： Heinrich KW,Wolfer J,Hong D,LeBlanc M,Sussman MR

更新日期：2012-06-01 00:00:00

abstract：:Protein dephosphorylation by the serine/threonine protein phosphatase 2A (PP2A) modulates a broad array of cellular functions. PP2A normally acts as a heterotrimeric holoenzyme complex comprising a catalytic subunit bound by regulatory A and B subunits. Characterization of the regulatory A subunit isoforms (ROOTS CURL...

journal_title：Plant physiology

authors： Blakeslee JJ,Zhou HW,Heath JT,Skottke KR,Barrios JA,Liu SY,DeLong A

更新日期：2008-02-01 00:00:00

abstract：:Root size and architecture are important crop plant traits, as they determine access to water and soil nutrients. The plant hormone cytokinin is a negative regulator of root growth and branching. Here, we generated transgenic barley (Hordeum vulgare) plants with an enlarged root system by enhancing cytokinin degradati...

journal_title：Plant physiology

authors： Ramireddy E,Hosseini SA,Eggert K,Gillandt S,Gnad H,von Wirén N,Schmülling T

更新日期：2018-07-01 00:00:00

abstract：:The pattern of endoproteolytic activities occurring during wheat (Triticum aestivum, cultivar Chinese Spring) grain development was investigated. Total endoprotease activity, assayed in solution with azocasein as a substrate, increased during the early stages of grain development to reach a maximum at 15 d postanthesi...

journal_title：Plant physiology

authors： Dominguez F,Cejudo FJ

更新日期：1996-11-01 00:00:00

abstract：:The secretion of alpha-amylase from single isolated (Hordeum vulgare L. cv Himalaya) aleurone layers was studied in an automated flow-through apparatus. The apparatus, consisting of a modified sample analyzer linked to a chart recorder, automatically samples the flow-through medium at 1 minute intervals and assays for...

journal_title：Plant physiology

authors： Moll BA,Jones RL

更新日期：1982-10-01 00:00:00

abstract：:Combined gas chromatography-mass spectrometry has been used to identify indole-3-methanol in a purified buffer extract from etiolated seedlings of Pinus sylvestris L. Quantitative estimates obtained by high performance liquid chromatography with fluorescence detection, corrected for losses occurring during purificatio...

journal_title：Plant physiology

authors： Sundberg B,Sandberg G,Jensen E

更新日期：1985-04-01 00:00:00

abstract：:Prenylated Rab acceptors (PRAs), members of the Ypt-interacting protein family of small membrane proteins, are thought to aid the targeting of prenylated Rabs to their respective endomembrane compartments. In plants, the Arabidopsis (Arabidopsis thaliana) PRA1 family contains 19 members that display varying degrees of...

journal_title：Plant physiology

authors： Lee MH,Jung C,Lee J,Kim SY,Lee Y,Hwang I

更新日期：2011-10-01 00:00:00

abstract：:Protein phosphorylation, catalyzed by the opposing actions of protein kinases and phosphatases, is a cornerstone of cellular signaling and regulation. Since their discovery, protein phosphatases have emerged as highly regulated enzymes with specificity that rivals their counteracting kinase partners. However, despite ...

journal_title：Plant physiology

authors： Uhrig RG,Moorhead GB

更新日期：2011-12-01 00:00:00

abstract：:trans-Acting small interfering RNAs (tasiRNAs) participate in the regulation of organ morphogenesis and determination of developmental timing in plants by down-regulating target genes through mRNA cleavage. The production of tasiRNAs is triggered by microRNA173 (miR173) and other specific microRNA-mediated cleavage of...

journal_title：Plant physiology

authors： Yoshikawa M,Iki T,Numa H,Miyashita K,Meshi T,Ishikawa M

更新日期：2016-05-01 00:00:00

abstract：:Environmental stresses frequently decrease plant fertility. In Arabidopsis, the effect of salt stress on reproduction was examined using plants grown in hydroponic medium. Salt stress inhibited microsporogenesis and stamen filament elongation. Because plants grown in hydroponic media can be rapidly and transiently str...

journal_title：Plant physiology

authors： Sun K,Hunt K,Hauser BA

更新日期：2004-08-01 00:00:00

abstract：:We have previously shown that in Nicotiana sylvestris cytoplasmic male-sterile (CMS) mutants where the mtDNA lacks the nad7 gene coding for a subunit of respiratory Complex I (NADH:ubiquinone oxidoreductase, EC 1.6.5.3), glycine (Gly) oxidation was lower than in the wild type and insensitive to rotenone, suggesting Co...

journal_title：Plant physiology

authors： Sabar M,De Paepe R,de Kouchkovsky Y

更新日期：2000-11-01 00:00:00

abstract：:Pyruvate dehydrogenase complex is associated with intact chloroplasts and mitochondria of 9-day-old Pisum sativum L. seedlings. The ratio of the mitochondrial complex to the chloroplast complex activities is about 3 to 1. Maximal rates observed for chloroplast pyruvate dehydrogenase complex activity ranged from 6 to 9...

journal_title：Plant physiology

authors： Williams M,Randall DD

更新日期：1979-12-01 00:00:00

abstract：:As regulators of gene expression in multicellular organisms, microRNAs (miRNAs) are crucial for growth and development. Although a plethora of factors involved in their biogenesis and action in Arabidopsis (Arabidopsis thaliana) has been described, these processes and their fine-tuning are not fully understood. Here, ...

journal_title：Plant physiology

authors： Lang PLM,Christie MD,Dogan ES,Schwab R,Hagmann J,van de Weyer AL,Scacchi E,Weigel D

更新日期：2018-01-01 00:00:00