Methyl-CpG-binding domain protein MBD7 is required for active DNA demethylation in Arabidopsis.

Abstract:

:Although researchers have established that DNA methylation and active demethylation are dynamically regulated in plant cells, the molecular mechanism for the regulation of active DNA demethylation is not well understood. By using an Arabidopsis (Arabidopsis thaliana) line expressing the Promoter RESPONSIVE TO DEHYDRATION 29A:LUCIFERASE (ProRD29A:LUC) and Promoter cauliflower mosaic virus 35S:NEOMYCIN PHOSPHOTRANSFERASE II (Pro35S:NPTII) transgenes, we isolated an mbd7 (for methyl-CpG-binding domain protein7) mutant. The mbd7 mutation causes an inactivation of the Pro35S:NPTII transgene but does not affect the expression of the ProRD29A:LUC transgene. The silencing of the Pro35S:NPTII reporter gene is associated with DNA hypermethylation of the reporter gene. MBD7 interacts physically with REPRESSOR OF SILENCING5/INCREASED DNA METHYLATION2, a protein in the small heat shock protein family. MBD7 prefers to target the genomic loci with high densities of DNA methylation around chromocenters. The Gypsy-type long terminal repeat retrotransposons mainly distributed around chromocenters are most affected by mbd7 in all transposons. Our results suggest that MBD7 is required for active DNA demethylation and antisilencing of the genomic loci with high densities of DNA methylation in Arabidopsis.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Wang C,Dong X,Jin D,Zhao Y,Xie S,Li X,He X,Lang Z,Lai J,Zhu JK,Gong Z

doi

10.1104/pp.114.252106

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

905-14

issue

3

eissn

0032-0889

issn

1532-2548

pii

pp.114.252106

journal_volume

167

pub_type

杂志文章
  • Down-regulation of Kelch domain-containing F-box protein in Arabidopsis enhances the production of (poly)phenols and tolerance to ultraviolet radiation.

    abstract::Phenylpropanoid biosynthesis in plants engenders myriad phenolics with diverse biological functions. Phenylalanine ammonia-lyase (PAL) is the first committed enzyme in the pathway, directing primary metabolic flux into a phenylpropanoid branch. Previously, we demonstrated that the Arabidopsis (Arabidopsis thaliana) Ke...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.249136

    authors: Zhang X,Gou M,Guo C,Yang H,Liu CJ

    更新日期:2015-02-01 00:00:00

  • Reconfiguration of the achene and receptacle metabolic networks during strawberry fruit development.

    abstract::The anatomy of strawberry (Fragaria x ananassa) fruit, in which the achene is found on the outer part of the fruit, makes it an excellent species for studying the regulation of fruit development. It can provide a model for the cross talk between primary and secondary metabolism, whose role is of pivotal importance in ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.120691

    authors: Fait A,Hanhineva K,Beleggia R,Dai N,Rogachev I,Nikiforova VJ,Fernie AR,Aharoni A

    更新日期:2008-10-01 00:00:00

  • Plant body weight-induced secondary growth in Arabidopsis and its transcription phenotype revealed by whole-transcriptome profiling.

    abstract::Wood is an important raw material and environmentally cost-effective renewable source of energy. However, the molecular biology of wood formation (i.e. secondary growth) is surprisingly understudied. A novel experimental system was employed to study the molecular regulation of secondary xylem formation in Arabidopsis....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.038844

    authors: Ko JH,Han KH,Park S,Yang J

    更新日期:2004-06-01 00:00:00

  • Phloem loading in two Scrophulariaceae species. What can drive symplastic flow via plasmodesmata?

    abstract::To determine the driving forces for symplastic sugar flux between mesophyll and phloem, gradients of sugar concentrations and osmotic pressure were studied in leaf tissues of two Scrophulariaceae species, Alonsoa meridionalis and Asarina barclaiana. A. meridionalis has a typical symplastic configuration of minor-vein ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.068312

    authors: Voitsekhovskaja OV,Koroleva OA,Batashev DR,Knop C,Tomos AD,Gamalei YV,Heldt HW,Lohaus G

    更新日期:2006-01-01 00:00:00

  • Characterization and subcellular compartmentation of recombinant 4-hydroxyphenylpyruvate dioxygenase from Arabidopsis in transgenic tobacco.

    abstract::4-Hydroxyphenylpyruvate dioxygenase (4HPPD) catalyzes the formation of homogentisate (2,5-dihydroxyphenylacetate) from p-hydroxyphenylpyruvate and molecular oxygen. In plants this enzyme activity is involved in two distinct metabolic processes, the biosynthesis of prenylquinones and the catabolism of tyrosine. We repo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.119.4.1507

    authors: Garcia I,Rodgers M,Pepin R,Hsieh TF,Matringe M

    更新日期:1999-04-01 00:00:00

  • Rotational streaming in fiber cells and its role in translocation.

    abstract::All visible protoplasmic streaming in sections of various plant stems was reversibly stopped by 2,4-dinitrophenol (DNP). Sections contained epidermal, cortical, and fiber cell types. Cells treated with DNP retained their semipermeability as evidenced by their plasmolysis in sucrose solutions. Washing out the DNP resul...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.10.1648

    authors: Worley JF

    更新日期:1968-10-01 00:00:00

  • Light-Stimulated Carotenoid Biosynthesis during Transformation of Maize Etioplasts Is Regulated by Increased Activity of Isopentenyl Pyrophosphate Isomerase.

    abstract::Light-stimulated carotenoid biosynthesis associated with the transformation of etioplasts to chloroplasts was investigated after dark-grown maize (Zea mays) seedlings were transferred into light. These studies focused on the enzymes of the pathway to detect those enzyme activities that were stimulated in the light and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.2.529

    authors: Albrecht M,Sandmann G

    更新日期:1994-06-01 00:00:00

  • Role of roots in regulating the growth rate and cytokinin content in leaves.

    abstract::The role of roots in the enhancement of cytokinin content and leaf growth of Phaseolus vulgaris plants after decapitation and partial defoliation was investigated. Partial excision of the roots of plants which were decapitated above the primary leaf node resulted in a reduction of leaf growth and soluble proteins accu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.1.76

    authors: Carmi A,Staden JV

    更新日期:1983-09-01 00:00:00

  • Changes in biochemical composition of the cell wall of the cotton fiber during development.

    abstract::The composition of the cell wall of the cotton fiber (Gossypium hirsutum L. Acala SJ-1) has been studied from the early stages of elongation (5 days postanthesis) through the period of secondary wall formation, using cell walls derived both from fibers developing on the plant and from fibers obtained from excised, cul...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.6.1088

    authors: Meinert MC,Delmer DP

    更新日期:1977-06-01 00:00:00

  • Relationship between Nitrate Uptake, Flux, and Reduction and the Accumulation of Reduced Nitrogen in Maize (Zea mays L.): II. EFFECT OF NUTRIENT NITRATE CONCENTRATION.

    abstract::Two maize hybrids were grown under growth chamber conditions on solution or vermiculite medium that contained 2.5, 7.5, or 15 millimolar nitrate. The objectives were to determine: (a) the effect of nitrate supply on N metabolism and growth and (b) the interrelationship between nitrate uptake, flux, and reduction on th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.6.1184

    authors: Reed AJ,Hageman RH

    更新日期:1980-12-01 00:00:00

  • Studies on the 70S Ribosomal Content of a Plastid Mutant in Gossypium hirsutum.

    abstract::Analysis of a mutation in cotton (Gossypium hirsutum), which is maternally inherited, revealed that the completely white sectors of leaves were deficient in the 70S class ribosomes, whereas the yellow sectors exhibited the same level of the latter as in the green leaves. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.6.1138

    authors: Katterman FR,Endrizzi JE

    更新日期:1973-06-01 00:00:00

  • A 2-Oxoglutarate-Dependent Dioxygenase Mediates the Biosynthesis of Glucoraphasatin in Radish.

    abstract::Glucosinolates (GSLs) are secondary metabolites whose degradation products confer intrinsic flavors and aromas to Brassicaceae vegetables. Several structures of GSLs are known in the Brassicaceae, and the biosynthetic pathway and regulatory networks have been elucidated in Arabidopsis (Arabidopsis thaliana). GSLs are ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01814

    authors: Kakizaki T,Kitashiba H,Zou Z,Li F,Fukino N,Ohara T,Nishio T,Ishida M

    更新日期:2017-03-01 00:00:00

  • Isolation and characterization of glutathione S-transferase isozymes from sorghum.

    abstract::Two glutathione S-transferase (GST) isozymes, A1/A1 and B1/B2, were purified from etiolated, O-1,3-dioxolan-2-yl-methyl-2,2,2, -trifluoro-4'-chloroacetophenone-oxime-treated sorghum (Sorghum bicolor L. Moench) shoots. GST A1/A1, a constitutively expressed homodimer, had a subunit molecular mass of 26 kD and an isoelec...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.3.877

    authors: Gronwald JW,Plaisance KL

    更新日期:1998-07-01 00:00:00

  • An auxin-inducible element in soybean SAUR promoters.

    abstract::The soybean SAUR (Small Auxin-Up RNA) genes are transcriptionally induced by exogenous auxins within a few minutes after hormone application. This response is specifically induced by auxins primarily in epidermal and cortical cells within elongation zones of hypocotyls and epicotyls. We have previously shown that an 8...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.1.37

    authors: Li Y,Liu ZB,Shi X,Hagen G,Guilfoyle TJ

    更新日期:1994-09-01 00:00:00

  • Photographic survey of the occurrence of bundle-sheath extensions in deciduous dicots.

    abstract::In a survey of over 300 nonevergreen dicots in 69 families, many species were found to have translucent patterns attributed to the presence of bundle-sheath extensions (BSE) on the small and ultimate veinlets. The BSE have been shown by others to inhibit transverse air movement within leaves, and it has been suggested...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.4.1677

    authors: McClendon JH

    更新日期:1992-08-01 00:00:00

  • Modification of cytokinins by cauliflower microsomal enzymes.

    abstract::N(6)-(Delta(2)-isopentenyl)Adenine and N(6)-(Delta(2)-isopentenyl)adenosine were hydroxylated, respectively, to 6-(4-hydroxy-3-methyl-trans-2-butenylamino)purine and 6-(4-hydroxy-3-methyl-trans-2-butenylamino) -9-beta-ribofuranosylpurine in the presence of NADPH and the microsomal fraction from cauliflowers (Brassica ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.2.442

    authors: Chen CM,Leisner SM

    更新日期:1984-06-01 00:00:00

  • Metabolism of tween-Fatty Acid esters by cultured soybean cells : kinetics of incorporation into lipids, subsequent turnover, and associated changes in endogenous Fatty Acid synthesis.

    abstract::Uptake of Tween-fatty acid esters and incorporation of the fatty acids into lipids by soybean (Glycine max [L.] Merr.) suspension cultures was investigated, together with subsequent turnover of the incorporated fatty acids and associated changes in endogenous fatty acid synthesis. Tween uptake was saturable, and fatty...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.3.780

    authors: Terzaghi WB

    更新日期:1986-11-01 00:00:00

  • Linear sucrose transport in protoplasts from developing soybean cotyledons.

    abstract::Previous studies with isolated soybean cotyledon protoplasts revealed the presence of a saturable, simple diffusion, and nonsaturating carrier-mediated uptake of sucrose into soybean cotyledon cells. A proton/sucrose cotransport may be involved in the saturable sucrose uptake (Lin et al. 1984 Plant Physiol 75: 936-940...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.3.649

    authors: Lin W

    更新日期:1985-07-01 00:00:00

  • Requirement for the H phosphoprotein in photosystem II of Chlamydomonas reinhardtii.

    abstract::To dissect the expression of the psbB gene cluster of the Chlamydomonas reinhardtii chloroplast genome and to assess the role of the photosystem II H-phosphoprotein (PSII-H) in the biogenesis and/or stabilization of PSII, an aadA gene cassette conferring spectinomycin resistance was employed for mutagenesis. Disruptio...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.4.1359

    authors: Summer EJ,Schmid VH,Bruns BU,Schmidt GW

    更新日期:1997-04-01 00:00:00

  • Procedures for the Generation of Mature Chlamydomonas reinhardtii Zygotes for Molecular and Biochemical Analyses.

    abstract::Zygotes represent an important stage in the sexual cycle of the unicellular green alga Chlamydomonas reinhardtii. To study zygote germination at a molecular level, a protocol was elaborated for the generation of zygotes in large quantities and a method was developed for the extraction from zygotes of RNA that could be...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.2.512

    authors: Wegener D,Treier U,Beck CF

    更新日期:1989-06-01 00:00:00

  • Auxin Conjugation by Tobacco Mesophyll Protoplasts : Correlations between Auxin Cytotoxicity under Low Density Growth Conditions and Induction of Conjugation Processes at High Density.

    abstract::Auxin induction of the proliferation of Nicotiana tabacum (cv Xanthi) mesophyll protoplasts and of protoplast-derived cells was studied. The growth-promoting properties and cytotoxicities at high concentrations of IAA and naphthaleneacetic acid were strongly affected by cell density. The induction of growth by 2,4-dic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.1.54

    authors: Caboche M,Aranda G,Poll AM,Huet JC,Leguay JJ

    更新日期:1984-05-01 00:00:00

  • Reduced expression of succinyl-coenzyme A ligase can be compensated for by up-regulation of the gamma-aminobutyrate shunt in illuminated tomato leaves.

    abstract::Increasing experimental evidence suggests that the tricarboxylic acid cycle in plants is of greater importance in illuminated photosynthetic tissues than previously thought. In this study, transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the beta-subunit of succinyl-coenzyme A ligase in either ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.103101

    authors: Studart-Guimarães C,Fait A,Nunes-Nesi A,Carrari F,Usadel B,Fernie AR

    更新日期:2007-11-01 00:00:00

  • Intracellular beta-carbonic anhydrase of the unicellular green alga Coccomyxa. Cloning of the cdna and characterization of the functional enzyme overexpressed in Escherichia coli.

    abstract::Carbonic anhydrase (CA) (EC 4.2.1.1) enzymes catalyze the reversible hydration of CO2, a reaction that is important in many physiological processes. We have cloned and sequenced a full-length cDNA encoding an intracellular beta-CA from the unicellular green alga Coccomyxa. Nucleotide sequence data show that the isolat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.4.1341

    authors: Hiltonen T,Björkbacka H,Forsman C,Clarke AK,Samuelsson G

    更新日期:1998-08-01 00:00:00

  • Quantifying Apoplastic Flux through Red Pine Root Systems Using Trisodium, 3-hydroxy-5,8,10-pyrenetrisulfonate.

    abstract::The fluorescent compound trisodium, 3-hydroxy-5,8,10-pyrenetrisulfonate (PTS) was used to quantify the apoplastic flux through red pine (Pinus resinosa Ait.) root systems-that portion of the total water flux reaching the xylem without ever crossing a semipermeable membrane. Flow was induced by pressure through detoppe...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.1.21

    authors: Hanson PJ,Sucoff EI,Markhart AH

    更新日期:1985-01-01 00:00:00

  • Inactivation of pea leaf chloroplastic and cytoplasmic glucose 6-phosphate dehydrogenases by light and dithiothreitol.

    abstract::Chloroplastic and cytoplasmic forms of pea (Pisum sativum L.) leaf glucose-6-P dehydrogenase can be separated by gel electrophoresis. Both forms are found in etiolated seedlings. When light-grown plants are illuminated or when crude extracts are treated with dithiothreitol, the chloroplastic and cytoplasmic forms of t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.6.835

    authors: Anderson LE,Ng TC,Park KE

    更新日期:1974-06-01 00:00:00

  • Expression of allene oxide synthase determines defense gene activation in tomato.

    abstract::Allene oxide synthase (AOS; hydroperoxide dehydratase; EC 4.2.1.92) catalyzes the first step in the biosynthesis of jasmonic acid from lipoxygenase-derived hydroperoxides of free fatty acids. Using the AOS cDNA from tomato (Lycopersicon esculentum), in which the role of jasmonic acid in wound-induced defense gene acti...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.4.1335

    authors: Sivasankar S,Sheldrick B,Rothstein SJ

    更新日期:2000-04-01 00:00:00

  • Molecular cloning and characterization of the cDNA coding for the biotin-containing subunit of the chloroplastic acetyl-coenzyme A carboxylase.

    abstract::We report the molecular cloning and sequence of the cDNA coding for the biotin-containing subunit of the chloroplastic acetylcoenzyme A (CoA) carboxylase (ACCase) of Arabidopsis thaliana (CAC1). The 3' end of the CAC1 sequence, coding for a peptide of 94 amino acids, which includes a putative biotinylation motif, was ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.2.619

    authors: Choi JK,Yu F,Wurtele ES,Nikolau BJ

    更新日期:1995-10-01 00:00:00

  • Chemical Composition of Hypodermal and Endodermal Cell Walls and Xylem Vessels Isolated from Clivia miniata (Identification of the Biopolymers Lignin and Suberin).

    abstract::The occurrence of the biopolymers lignin and suberin was investigated with hypodermal (HCW) and endodermal cell walls (ECW) and xylem vessels (XV) isolated from Clivia miniata Reg. roots. Both biopolymers were detected in HCW and ECW, whereas in XV, typical aliphatic suberin monomers were missing and only representati...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.4.1223

    authors: Zeier J,Schreiber L

    更新日期:1997-04-01 00:00:00

  • An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine.

    abstract::In contrast to angiosperms, pines and other gymnosperms form well-developed suspensors in somatic embryogenic cultures. This creates a useful system to study suspensor biology. In a study of gene expression during the early stages of conifer embryogenesis, we identified a transcript, PtNIP1;1, that is abundant in imma...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Ciavatta VT,Morillon R,Pullman GS,Chrispeels MJ,Cairney J

    更新日期:2001-12-01 00:00:00

  • Diurnal trends in net photosynthetic rate and carbohydrate levels of soybean leaves.

    abstract::A study was made of diurnal trends in net photosynthetic rate and carbohydrate levels of unifoliolate leaves of soybean (Glycine max L. Merrill) under constant environmental conditions (50,000-lux light intensity, 24.5 C air temperature, 60% relative humidity, and 300 microliters of CO(2) per liter of air).Net photosy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.5.871

    authors: Upmeyer DJ,Koller HR

    更新日期:1973-05-01 00:00:00