Intracellular beta-carbonic anhydrase of the unicellular green alga Coccomyxa. Cloning of the cdna and characterization of the functional enzyme overexpressed in Escherichia coli.

abstract：:Intact spinach (Spinacia oleracea) chloroplasts, thylakoid membranes, and inside-out or right-side-out thylakoid vesicles have been characterized by flow cytometry with respect to forward angle light scatter, right angle light scatter, and chlorophyll fluorescence. Analysis of intact chloroplasts with respect to forwa...

journal_title：Plant physiology

authors： Schröder WP,Petit PX

更新日期：1992-11-01 00:00:00

abstract：:The seasonal development of adaxial Prunus laurocerasus leaf surfaces was studied using newly developed methods for the mechanical removal of epicuticular waxes. During epidermal cell expansion, more than 50 microg leaf(-1) of alkyl acetates accumulated within 10 d, forming an epicuticular wax film approximately 30 nm...

journal_title：Plant physiology

authors： Jetter R,Schäffer S

更新日期：2001-08-01 00:00:00

abstract：:Angiosperms requires light for multiple aspects of chloroplast development, including chlorophyll synthesis and induction of expression of the mRNAs encoding the major polypeptides of the light-harvesting complex of photosystem II (Lhcb genes). In contrast, many conifers, including pines, firs, and spruces, can accumu...

journal_title：Plant physiology

authors： Peer W,Silverthorne J,Peters JL

更新日期：1996-06-01 00:00:00

abstract：:Treatment of grass leaves with either a purified pectin lyase of Aspergillus japonicus or a purified xylanase of Trichoderma viride could lead to the isolation of some single leaf cells. However, a mixture of pectin lyase and xylanase brought about more rapid isolation of single cells than did either of the two enzyme...

journal_title：Plant physiology

authors： Ishii S

更新日期：1984-12-01 00:00:00

abstract：:Previous studies with isolated soybean cotyledon protoplasts revealed the presence of a saturable, simple diffusion, and nonsaturating carrier-mediated uptake of sucrose into soybean cotyledon cells. A proton/sucrose cotransport may be involved in the saturable sucrose uptake (Lin et al. 1984 Plant Physiol 75: 936-940...

journal_title：Plant physiology

authors： Lin W

更新日期：1985-07-01 00:00:00

abstract：:More volatile organic carbon is lost from plants as isoprene than any other molecule. This flux of carbon to the atmosphere affects atmospheric chemistry and can serve as a substrate for ozone production in polluted air. Isoprene synthesis may help leaves cope with heatflecks and active oxygen species. Isoprene syntha...

journal_title：Plant physiology

authors： Wolfertz M,Sharkey TD,Boland W,Kühnemann F

更新日期：2004-08-01 00:00:00

abstract：:The unicellular green alga Chlamydomonas reinhardtii is a widely used model organism for studies of oxygenic photosynthesis in eukaryotes. Here we describe the development of a resource for functional genomics of photosynthesis using insertional mutagenesis of the Chlamydomonas nuclear genome. Chlamydomonas cells were...

journal_title：Plant physiology

authors： Dent RM,Haglund CM,Chin BL,Kobayashi MC,Niyogi KK

更新日期：2005-02-01 00:00:00

abstract：:ATP, which is present in the extracellular matrix of multicellular organisms and in the extracellular fluid of unicellular organisms, has been shown to function as a signaling molecule in animals. The concentration of extracellular ATP (xATP) is known to be functionally modulated in part by ectoapyrases, membrane-asso...

journal_title：Plant physiology

authors： Thomas C,Sun Y,Naus K,Lloyd A,Roux S

更新日期：1999-02-01 00:00:00

abstract：:Plant viruses utilize the vascular system for systemic movement. The plant vascular network also transports water, photosynthates, and signaling molecules and is essential for plant growth. However, the molecular mechanisms governing vascular development and patterning are still largely unknown. From viral transport s...

journal_title：Plant physiology

authors： Jin H,Li S,Villegas A Jr

更新日期：2006-10-01 00:00:00

abstract：:Using the gibberellin (GA) biosynthetic inhibitor Uniconazol, we determined that det1, a mutant that no longer requires light to be germinated, still requires GA synthesis for germination. This result suggests that dark inhibition of germination in Arabidopsis may be due to inhibition of GA synthesis by the DET1 gene ...

journal_title：Plant physiology

authors： Nambara E,Akazawa T,McCourt P

更新日期：1991-10-01 00:00:00

abstract：:To test the hypothesis that multiple integrated root phenotypes would co-optimize drought tolerance, we phenotyped the root anatomy and architecture of 400 mature maize (Zea mays) genotypes under well-watered and water-stressed conditions in the field. We found substantial variation in all 23 root phenes measured. A p...

journal_title：Plant physiology

authors： Klein SP,Schneider HM,Perkins AC,Brown KM,Lynch JP

更新日期：2020-07-01 00:00:00

abstract：:Sucrose:sucrose 1-fructosyltransferase (1-SST), an enzyme involved in fructan biosynthesis, was purified to homogeneity from tubers of Helianthus tuberosus that were harvested in the accumulation phase. Gel filtration under native conditions predicted a molecular mass of about 67 kD. Electrophoresis or gel filtration ...

journal_title：Plant physiology

authors： Koops AJ,Jonker HH

更新日期：1996-04-01 00:00:00

abstract：:Superoxide dismutase was purified from pea (Pisum sativum L., cv. Wando) seeds and corn (Zea mays L., cv. Michigan 500) seedlings. The purified pea enzyme eluting as a single peak from gel exclusion chromatography columns contained the three electrophoretically distinct bands of superoxide dismutase characterizing the...

journal_title：Plant physiology

authors： Giannopolitis CN,Ries SK

更新日期：1977-02-01 00:00:00

abstract：:Respiration utilizing NAD-linked substrates in mitochondria isolated from cotyledons of etiolated peas (Pisum sativum L. var. Homesteader) by sucrose density gradient centrifugation exhibited resistance to rotenone. The inhibited rate of alpha-ketoglutarate oxidation was equivalent to the recovered rate of malate oxid...

journal_title：Plant physiology

authors： Johnson-Flanagan AM,Spencer MS

更新日期：1981-12-01 00:00:00

abstract：:Cytokinin proved to be a controlling factor in sieve tube regeneration around wounded collateral bundles in an in vivo system in which the endogenous cytokinin level had been minimized. Both kinetin and zeatin were applied in aqueous solution to the bases of excised, mature internodes of Coleus blumei Benth. that had ...

journal_title：Plant physiology

authors： Aloni R,Baum SF,Peterson CA

更新日期：1990-07-01 00:00:00

abstract：:Doubling the concentration of atmospheric CO2 often inhibits plant respiration, but the mechanistic basis of this effect is unknown. We investigated the direct effects of increasing the concentration of CO2 by 360 [mu]L L-1 above ambient on O2 uptake in isolated mitochondria from soybean (Glycine max L. cv Ransom) cot...

journal_title：Plant physiology

authors： Gonzalez-Meler MA,Ribas-Carbo M,Siedow JN,Drake BG

更新日期：1996-11-01 00:00:00

abstract：:Photosynthetic fixation of (14)CO(2) in the bean Phaseolus vulgaris, cv. Pencil Pod Black Wax, resulted in the appearance of labeled compounds that were characterized as phosphoserine and phosphohydroxypyruvate by chromatographic separation and by the synthesis of chemical derivatives. In (14)CO(2)/(12)CO(2) pulse-cha...

journal_title：Plant physiology

authors： Daley LS,Bidwell RG

更新日期：1977-07-01 00:00:00

abstract：:The reducing agents, potassium ferrocyanide, beta-mercaptoethylamine, cysteine, reduced DPN, ferrous sulfate, methyl viologen and ascorbic acid caused the expansion in darkness of disks of primary leaf tissue cut from dark-grown bean plants. The reducing agents interacted synergistically with low irradiances of red ra...

journal_title：Plant physiology

authors： Klein RM,Edsall PC

更新日期：1966-06-01 00:00:00

abstract：:42K+ and 86Rb+ were used to determine the subcellular distribution of potassium in Chlamydomonas reinhardtii by compartmental analysis. In both wild type and a mutant strain, three distinct compartments (referred to as I, II, and III) were apparent. Using 42K+, we found that these had half-lives for K+ exchange of 1.0...

journal_title：Plant physiology

authors： Malhotra B,Glass A

更新日期：1995-08-01 00:00:00

abstract：:Lipid-linked oligosaccharides were synthesized with the particulate enzyme preparation from mung bean (Phaseolus aureus) seedlings in the presence of GDP-[(14)C] mannose. The oligosaccharides were released from the lipids by mild acid hydrolysis and purified by several passages on Biogel P-4 columns. Five different ol...

journal_title：Plant physiology

authors： Hori H,Elbein AD

更新日期：1982-07-01 00:00:00

abstract：:Excision of spinach (Spinacia oleracea L.) leaves had no effect on photosynthetic rates, but altered normal carbon partitioning to favor increased formation of starch and decreased formation of sucrose. The changes were evident within 2 hours after excision. Concurrently, leaf fructose-2,6-bisphosphate content increas...

journal_title：Plant physiology

authors： Huber SC,Bickett DM

更新日期：1984-02-01 00:00:00

abstract：:Nitrogen (N) is an essential requirement for kernel growth in maize (Zea mays); however, little is known about how N assimilates are metabolized in young earshoots during seed development. The objective of this study was to assess amino acid metabolism in cob and spikelet tissues during the critical 2 weeks following ...

journal_title：Plant physiology

authors： Seebauer JR,Moose SP,Fabbri BJ,Crossland LD,Below FE

更新日期：2004-12-01 00:00:00

abstract：:Phycoerythrin, a photosynthetic accessory pigment, was isolated from Porphyridium cruentum and examined by electron microscopy and disc gel electrophoresis. The absorption monomer, with maxima at 563, 545, and a shoulder at 500 nm, has a molecular weight of about 300,000. With phosphotungstic acid staining it appears ...

journal_title：Plant physiology

authors： Gantt E

更新日期：1969-11-01 00:00:00

abstract：:We conducted a comprehensive metabolic phenotyping of potato (Solanum tuberosum L. cv Desiree) tuber tissue that had been modified either by transgenesis or exposure to different environmental conditions using a recently developed gas chromatography-mass spectrometry profiling protocol. Applying this technique, we wer...

journal_title：Plant physiology

authors： Roessner U,Willmitzer L,Fernie AR

更新日期：2001-11-01 00:00:00

abstract：:Guard cells represent a highly differentiated cell type within the epidermis of plant leaves and stems. They respond to many endogenous and environmental signals and thereby modify the size of the stomatal pore they surround. We identified a novel gene that is highly expressed in guard cells of potato (Solanum tuberos...

journal_title：Plant physiology

authors： Menke U,Renault N,Mueller-Roeber B

更新日期：2000-03-01 00:00:00

abstract：:A population of M2 seedlings of Arabidopsis thaliana was screened for mutants that were insensitive to the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC). Several independent lines were obtained and proved insensitive to both ACC and ethylene. Two lines were identified as alleles of a single recessive muta...

journal_title：Plant physiology

authors： Van Der Straeten D,Djudzman A,Van Caeneghem W,Smalle J,Van Montagu M

更新日期：1993-06-01 00:00:00

abstract：:Phenolic compounds are frequently reported to be the main components of root exudates in response to iron (Fe) deficiency in Strategy I plants, but relatively little is known about their function. Here, we show that removal of secreted phenolics from the root-bathing solution almost completely inhibited the reutilizat...

journal_title：Plant physiology

authors： Jin CW,You GY,He YF,Tang C,Wu P,Zheng SJ

更新日期：2007-05-01 00:00:00

abstract：:Reactive oxygen species act as signaling molecules but can also directly provoke cellular damage by rapidly oxidizing cellular components, including lipids. We developed a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry-based quantitative method that allowed us to discriminate b...

journal_title：Plant physiology

authors： Triantaphylidès C,Krischke M,Hoeberichts FA,Ksas B,Gresser G,Havaux M,Van Breusegem F,Mueller MJ

更新日期：2008-10-01 00:00:00

abstract：:Logarithmic growth rates (either fresh or dry weight basis) of tobacco callus tissues grown on 10(-4) to 10(-1) mum cytokinin are increased if gibberellic acid (10(-3)-2 mum) is incorporated into the medium. At higher (1-10 mum) cytokinin concentrations gibberellic acid has little effect on growth rate but extends the...

journal_title：Plant physiology

authors： Helgeson JP,Upper CD

更新日期：1970-07-01 00:00:00

abstract：:Cell-type-specific gene expression is essential to distinguish between the numerous cell types of multicellular organism. Therefore, cell-type-specific gene expression is tightly regulated and for most genes RNA transcription is the central point of control. Thus, transcriptional reporters are broadly used markers for...

journal_title：Plant physiology

authors： Radoeva T,Ten Hove CA,Saiga S,Weijers D

更新日期：2016-06-01 00:00:00