Properties and Ultrastructure of Phycoerythrin From Porphyridium cruentum.

Abstract:

:Phycoerythrin, a photosynthetic accessory pigment, was isolated from Porphyridium cruentum and examined by electron microscopy and disc gel electrophoresis. The absorption monomer, with maxima at 563, 545, and a shoulder at 500 nm, has a molecular weight of about 300,000. With phosphotungstic acid staining it appears as a tightly structured disc-shaped particle possessing a mean diameter of 101 +/- 0.4A and height of 54 +/- 0.7A. The absorption maxima remained the same in glutaraldehyde fixed material, and in dimer and trimer aggregates. Treatment with sodium dodecyl sulfate caused a breakdown into smaller units accompanied by a loss of the 563 nm peak. It is suggested that this absorption monomer is the in vivo functional species and comparable to the phycocyanin hexamer, but structurally distinguishable at the ultrastructural level. It has been calculated that about 35 phycobiliprotein molecules can be contained within each phycobilisome. There are 1.4 x 10(3) chlorophyll molecules per phycobilisome, but not contained within it.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Gantt E

doi

10.1104/pp.44.11.1629

subject

Has Abstract

pub_date

1969-11-01 00:00:00

pages

1629-38

issue

11

eissn

0032-0889

issn

1532-2548

journal_volume

44

pub_type

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