Transcription Factor bHLH2 Represses CYSTEINE PROTEASE77 to Negatively Regulate Nodule Senescence.

Abstract:

:Legume-rhizobia symbiosis is a time-limited process due to the onset of senescence, which results in the degradation of host plant cells and symbiosomes. A number of transcription factors, proteases, and functional genes have been associated with nodule senescence; however, whether other proteases or transcription factors are involved in nodule senescence remains poorly understood. In this study, we identified an early nodule senescence mutant in Medicago truncatula, denoted basic helix-loop-helix transcription factor2 (bhlh2), that exhibits decreased nitrogenase activity, acceleration of plant programmed cell death (PCD), and accumulation of reactive oxygen species (ROS). The results suggest that MtbHLH2 plays a negative role in nodule senescence. Nodules of wild-type and bhlh2-TALEN mutant plants at 28 d postinoculation were used for transcriptome sequencing. The transcriptome data analysis identified a papain-like Cys protease gene, denoted MtCP77, that could serve as a potential target of MtbHLH2. Electrophoretic mobility shift assays and chromatin immunoprecipitation analysis demonstrated that MtbHLH2 directly binds to the promoter of MtCP77 to inhibit its expression. MtCP77 positively regulates nodule senescence by accelerating plant PCD and ROS accumulation. In addition, the expression of MtbHLH2 in the nodules gradually decreased from the meristematic zone to the nitrogen fixation zone, whereas the expression of MtCP77 showed enhancement. These results indicate that MtbHLH2 and MtCP77 have opposite functions in the regulation of nodule senescence. These results reveal significant roles for MtbHLH2 and MtCP77 in plant PCD, ROS accumulation, and nodule senescence, and improve our understanding of the regulation of the nodule senescence process.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Deng J,Zhu F,Liu J,Zhao Y,Wen J,Wang T,Dong J

doi

10.1104/pp.19.00574

subject

Has Abstract

pub_date

2019-12-01 00:00:00

pages

1683-1703

issue

4

eissn

0032-0889

issn

1532-2548

pii

pp.19.00574

journal_volume

181

pub_type

杂志文章
  • Modification of logarithmic growth rates of tobacco callus tissue by gibberellic Acid.

    abstract::Logarithmic growth rates (either fresh or dry weight basis) of tobacco callus tissues grown on 10(-4) to 10(-1) mum cytokinin are increased if gibberellic acid (10(-3)-2 mum) is incorporated into the medium. At higher (1-10 mum) cytokinin concentrations gibberellic acid has little effect on growth rate but extends the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.1.113

    authors: Helgeson JP,Upper CD

    更新日期:1970-07-01 00:00:00

  • Transmission of the Monocarpic Senescence Signal via the Xylem in Soybean.

    abstract::During monocarpic senescence in soybean (Glycine max [L.] Merrill cv. Anoka) there is a remobilization of nitrogen from the leaves to the seeds, and it has been hypothesized that this loss of nitrogen from the leaves induces foliar yellowing. The phloem in a small segment of the petiole between the pods and the target...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.4.754

    authors: Noodén LD,Murray BJ

    更新日期:1982-04-01 00:00:00

  • Iron-mediated control of the basic helix-loop-helix protein FER, a regulator of iron uptake in tomato.

    abstract::Root iron mobilization genes are induced by iron deficiency downstream of an unknown signaling mechanism. The FER gene, encoding a basic helix-loop-helix domain protein and putative transcription factor, is required for induction of iron mobilization genes in roots of tomato (Lycopersicon esculentum). To study upstrea...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.054270

    authors: Brumbarova T,Bauer P

    更新日期:2005-03-01 00:00:00

  • Tolerance of photosynthesis to high temperature in desert plants.

    abstract::Winter- and summertime-active desert annual species were grown at different temperatures to assess their capacity for photosynthetic acclimation. Thermal stability of photosynthesis was determined from responses of chlorophyll fluorescence to increased temperature. Photosynthesis in winter ephemerals grown at 28 degre...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.4.786

    authors: Downton WJ,Berry JA,Seemann JR

    更新日期:1984-04-01 00:00:00

  • Observations of hydrogen and oxygen isotopes in leaf water confirm the craig-gordon model under wide-ranging environmental conditions

    abstract::The Craig-Gordon evaporative enrichment model of the hydrogen (deltaD) and oxygen (delta(18)O) isotopes of water was tested in a controlled-environment gas exchange cuvette over a wide range (400 per thousand deltaD and 40 per thousand delta(18)O) of leaf waters. (Throughout this paper we use the term "leaf water" to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.4.1165

    authors: Roden JS,Ehleringer JR

    更新日期:1999-08-01 00:00:00

  • A novel phospholipase D of Arabidopsis that is activated by oleic acid and associated with the plasma membrane.

    abstract::Oleate-dependent phospholipase D (PLD; EC 3.1.4.4) has been reported in animal systems, but its molecular nature is unkown. Multiple PLDs have been characterized in plants, but none of the previously cloned PLDs exhibits the oleate-activated activity. Here, we describe the biochemical and molecular identification and ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Wang C,Wang X

    更新日期:2001-11-01 00:00:00

  • Oxidative Phosphorylation in Germinating Lettuce Seeds (Lactuca sativa) during the First Hours of Imbibition.

    abstract::Experiments with lettuce seeds during the first hours of imbibition showed that oxygen is necessary to sustain high adenine nucleotide ratios and consequently, energy charge values are higher than 0.8 as is usually the case in normally metabolizing tissues.The energy charge value (0.2) of dry seeds soaked in aerated w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.3.631

    authors: Hourmant A,Pradet A

    更新日期:1981-09-01 00:00:00

  • AtATM3 is involved in heavy metal resistance in Arabidopsis.

    abstract::AtATM3, an ATP-binding cassette transporter of Arabidopsis (Arabidopsis thaliana), is a mitochondrial protein involved in the biogenesis of iron-sulfur clusters and iron homeostasis in plants. Our gene expression analysis showed that AtATM3 is up-regulated in roots of plants treated with cadmium [Cd(II)] or lead (II);...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.074146

    authors: Kim DY,Bovet L,Kushnir S,Noh EW,Martinoia E,Lee Y

    更新日期:2006-03-01 00:00:00

  • Salicylic Acid in Rice (Biosynthesis, Conjugation, and Possible Role).

    abstract::Salicylic acid (SA) is a natural inducer of disease resistance in some dicotyledonous plants. Rice seedlings (Oryza sativa L.) had the highest levels of SA among all plants tested for SA content (between 0.01 and 37.19 [mu]g/g fresh weight). The second leaf of rice seedlings had slightly lower SA levels than any young...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.2.633

    authors: Silverman P,Seskar M,Kanter D,Schweizer P,Metraux JP,Raskin I

    更新日期:1995-06-01 00:00:00

  • Tailoring the specificity of a plant cystatin toward herbivorous insect digestive cysteine proteases by single mutations at positively selected amino acid sites.

    abstract::Plant cystatins, similar to other defense proteins, include hypervariable, positively selected amino acid sites presumably impacting their biological activity. Using 29 single mutants of the eighth domain of tomato (Solanum lycopersicum) multicystatin, SlCYS8, we assessed here the potential of site-directed mutagenesi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.115741

    authors: Goulet MC,Dallaire C,Vaillancourt LP,Khalf M,Badri AM,Preradov A,Duceppe MO,Goulet C,Cloutier C,Michaud D

    更新日期:2008-03-01 00:00:00

  • Nutritional Regulation of Organelle Biogenesis in Euglena: INDUCTION OF MICROBODIES.

    abstract::Exposure of dark grown resting Euglena to ethanol produced a transient increase in the specific activity of the glyoxysomal enzyme malate synthase. Enzyme specific activity increased during the first 24 hours of ethanol treatment and then declined. Light exposure or malate addition failed to increase enzyme specific a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.2.430

    authors: Horrum MA,Schwartzbach SD

    更新日期:1981-08-01 00:00:00

  • A digital diffusion porometer circuit.

    abstract::An automatic digital diffusion porometer circuit is described. The circuit is highly stable with respect to temperature and supply voltage. The device is capable of high timing accuracy over very short measurement intervals, so that measurements may be made rapidly without the danger of stomatal changes occurring duri...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.1.47

    authors: Fiscus EL

    更新日期:1974-01-01 00:00:00

  • Changes in the level of free and ester indol-3yl-acetic Acid in growing maize roots.

    abstract::The levels of free and ester-linked indole-3-acetic acid (IAA) in different parts of the maize root were measured using gas chromatography-mass spectrometry (selected ion monitoring). In roots of 2-day-old plants, the distribution of free and ester IAA differed both along the root and between stele and cortex. The lev...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.1.42

    authors: Saugy M,Pilet PE

    更新日期:1987-09-01 00:00:00

  • Regulation of light-harvesting chlorophyll protein biosynthesis in greening seedlings : a species comparison.

    abstract::The biosynthesis of the chlorophyll a/b binding protein associated with photosystem II (LHC-II) was characterized during light-induced greening of etiolated barley (Hordeum vulgare [L.] cv Boone), maize (Zea mays [L.] Pioneer 3148), pea (Pisum sativum [L.] cv Progress 9), and soybean (Glycine max [L.] Merr. cv Ransom ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.4.971

    authors: Mathis JN,Burkey KO

    更新日期:1987-12-01 00:00:00

  • Chilling-Induced Lipid Degradation in Cucumber (Cucumis sativa L. cv Hybrid C) Fruit.

    abstract::Chilling at 4 degrees C in the dark induced lipid degradation in cucumber (Cucumis sativa L.) fruit upon rewarming at 14 degrees C. Rates of ethane evolution by fruits rewarmed after 3 days of chilling were up to four-fold higher than those evolved by unchilled (14 degrees C) fruits (0.02-0.05 picomoles gram fresh wei...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.3.1049

    authors: Parkin KL,Kuo SJ

    更新日期:1989-07-01 00:00:00

  • Ozone-induced Loss of Intracellular Potassium Ion from Chlorella sorokiniana.

    abstract::The unicellular algae Chlorella sorokiniana was used as a model system to investigate the interaction of ozone with plant cell membranes. Ozone induces K(+) leakage from Chlorella sorokiniana similar to the electrolytic loss observed from many higher plants under stressful conditions. The kinetics of this leakage indi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.56.6.723

    authors: Chimiklis PE,Heath RL

    更新日期:1975-12-01 00:00:00

  • AtFXG1, an Arabidopsis gene encoding alpha-L-fucosidase active against fucosylated xyloglucan oligosaccharides.

    abstract::An alpha-L-fucosidase (EC 3.2.1.51) able to release the t-fucosyl residue from the side chain of xyloglucan oligosaccharides has been detected in the leaves of Arabidopsis plants. Moreover, an alpha-L-fucosidase with similar substrate specificity was purified from cabbage (Brassica oleracea) leaves to render a single ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: de La Torre F,Sampedro J,Zarra I,Revilla G

    更新日期:2002-01-01 00:00:00

  • Monoterpene metabolism. Cloning, expression, and characterization of (-)-isopiperitenol/(-)-carveol dehydrogenase of peppermint and spearmint.

    abstract::The essential oils of peppermint (Mentha x piperita) and spearmint (Mentha spicata) are distinguished by the oxygenation position on the p-menthane ring of the constitutive monoterpenes that is conferred by two regiospecific cytochrome P450 limonene-3- and limonene-6-hydroxylases. Following hydroxylation of limonene, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.053298

    authors: Ringer KL,Davis EM,Croteau R

    更新日期:2005-03-01 00:00:00

  • Voltage-sensor transitions of the inward-rectifying K+ channel KAT1 indicate a latching mechanism biased by hydration within the voltage sensor.

    abstract::The Kv-like (potassium voltage-dependent) K(+) channels at the plasma membrane, including the inward-rectifying KAT1 K(+) channel of Arabidopsis (Arabidopsis thaliana), are important targets for manipulating K(+) homeostasis in plants. Gating modification, especially, has been identified as a promising means by which ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.244319

    authors: Lefoulon C,Karnik R,Honsbein A,Gutla PV,Grefen C,Riedelsberger J,Poblete T,Dreyer I,Gonzalez W,Blatt MR

    更新日期:2014-10-01 00:00:00

  • Evaluation of h secretion relative to zeatin-induced growth of detached cucumber cotyledons.

    abstract::Cytokinins promote expansion of cotyledons detached from seedlings of more than a dozen species. The zeatin-enhanced expansion of cucumber (Cucumis sativus L. cv Marketer) cotyledons was investigated. In addition, whether acid secretion is involved in wall loosening accompanying such accelerated growth was evaluated. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.5.1470

    authors: Ross CW,Rayle DL

    更新日期:1982-11-01 00:00:00

  • Detecting mutants that have impaired photosynthesis by their increased level of fluorescence.

    abstract::A technique is described for detecting mutants of Chlamydomonas reinhardi that have impaired photosynthesis. It is based on the fact that when photosynthetic electron transport is blocked there may be an increase in the level of fluorescence similar to that obtained in the presence of an inhibitor of photosynthetic el...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.9.1284

    authors: Bennoun P,Levine RP

    更新日期:1967-09-01 00:00:00

  • Short-term leaf elongation kinetics of maize in response to salinity are independent of the root.

    abstract::The essentiality of roots to the short-term responses of leaf elongation to salinity was tested by removing the roots of maize (Zea mays L.) from the shoots and comparing the initial short-term response of leaf elongation to that with intact plants. Eightday-old seedlings growing in solution culture were treated with ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.3.965

    authors: Cramer GR,Bowman DC

    更新日期:1991-03-01 00:00:00

  • Trithorax group protein Oryza sativa Trithorax1 controls flowering time in rice via interaction with early heading date3.

    abstract::Trithorax group proteins are chromatin-remodeling factors that activate target gene expression by antagonistically functioning against the Polycomb group. In Arabidopsis (Arabidopsis thaliana), Arabidopsis Trithorax protein1 (ATX1) regulates flowering time and floral organ identity. Here, we observed that suppression ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.228049

    authors: Choi SC,Lee S,Kim SR,Lee YS,Liu C,Cao X,An G

    更新日期:2014-03-01 00:00:00

  • Photosynthesis in Fescue : III. RATES OF ELECTRON TRANSPORT IN A POLYPLOID SERIES OF TALL FESCUE PLANTS.

    abstract::Photosystem I electron transport activity has been found to be considerably higher in a decaploid tall fescue (Festuca arundinacea Schreb.) genotype as compared to a common hexaploid genotype. The decaploid genotype also displayed a higher photosystem whole chain (Photosystem II plus Photosystem I uncoupled) activity,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.5.1110

    authors: Krueger RW,Miles D

    更新日期:1981-11-01 00:00:00

  • K+-Selective inward-rectifying channels and apoplastic pH in barley roots

    abstract::Recent structure-function analysis of heterologously expressed K+-selective inward-rectifying channels (KIRCs) from plants has revealed that external protons can have opposite effects on different members of the same gene family. An important question is how the diverse response of KIRCs to apoplastic pH is reflected ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.1.331

    authors: Amtmann A,Jelitto TC,Sanders D

    更新日期:1999-05-01 00:00:00

  • The Pepper RING-Type E3 Ligase CaAIRF1 Regulates ABA and Drought Signaling via CaADIP1 Protein Phosphatase Degradation.

    abstract::Ubiquitin-mediated protein modification occurs at multiple steps of abscisic acid (ABA) signaling. Here, we sought proteins responsible for degradation of the pepper (Capsicum annuum) type 2C protein phosphatase CaADIP1 via the 26S proteasome system. We showed that the RING-type E3 ligase CaAIRF1 (Capsicum annuum ADIP...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01817

    authors: Lim CW,Baek W,Lee SC

    更新日期:2017-04-01 00:00:00

  • Characterization of two isoenzymes of lipoxygenase from bush beans.

    abstract::Two isoenzymes of lipoxygenase, a and b, have been obtained from bush beans (Phaseolus vulgaris) as electrophoretically homogeneous proteins. Both proteins have a molecular weight of 100,000, contain 1 atom of iron, and appear to be composed of a single peptide chain. However, these enzymes appear to differ in some ot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.4.695

    authors: Hurt GB,Axelrod B

    更新日期:1977-04-01 00:00:00

  • Factors involved in the opening of the hypocotyl hook of cotton and beans.

    abstract::Conditions influencing the opening of the bean (Phaseolus vulgaris L.) and cotton (Gossypium hirsutum L.) hypocotyl hook were defined. Such hooks were shown to undergo geotropic curvature; orientation of the hook with respect to gravity greatly affected the observed opening. Cotton and bean hooks behaved exactly oppos...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.45.5.548

    authors: Powell RD,Morgan PW

    更新日期:1970-05-01 00:00:00

  • Independent action of ELF3 and phyB to control hypocotyl elongation and flowering time.

    abstract::Light regulates various aspects of plant growth, and the photoreceptor phytochrome B (phyB) mediates many responses to red light. In a screen for Arabidopsis mutants with phenotypes similar to those of phyB mutants, we isolated two new elf3 mutants. One has weaker morphological phenotypes than previously identified el...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.4.1149

    authors: Reed JW,Nagpal P,Bastow RM,Solomon KS,Dowson-Day MJ,Elumalai RP,Millar AJ

    更新日期:2000-04-01 00:00:00

  • Developmental and light-regulated expression of individual members of the light-harvesting complex b gene family in Pinus palustris.

    abstract::Angiosperms requires light for multiple aspects of chloroplast development, including chlorophyll synthesis and induction of expression of the mRNAs encoding the major polypeptides of the light-harvesting complex of photosystem II (Lhcb genes). In contrast, many conifers, including pines, firs, and spruces, can accumu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.2.627

    authors: Peer W,Silverthorne J,Peters JL

    更新日期:1996-06-01 00:00:00