Characterization of two isoenzymes of lipoxygenase from bush beans.

Abstract:

:Two isoenzymes of lipoxygenase, a and b, have been obtained from bush beans (Phaseolus vulgaris) as electrophoretically homogeneous proteins. Both proteins have a molecular weight of 100,000, contain 1 atom of iron, and appear to be composed of a single peptide chain. However, these enzymes appear to differ in some other respects. Thus, lipoxygenase a has an isoelectric point of 6.03 while lipoxygenase b has a value of 5.57. Their pH optima are 5 to 7 and 6.5 to 7, respectively. Both lipoxygenase a and b, when acting on linoleic acid plus the product hydroperoxide, generate what are presumably keto-dienes with an absorption maximum at 280 nm. Whereas lipoxygenase a can catalyze this secondary reaction in the presence of O(2), lipoxygenase b does so only under anaerobic conditions. Lipoxygenase a is stimulated by Ca(2+) while lipoxygenase is not. An unexpected finding is the strong inhibition of lipoxygenase a by Mn(2+) (50% inhibition at 12.5 muM under standard reaction conditions). Lipoxygenase b is inhibited by Mn(2+) but only at concentrations about 250 times greater.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Hurt GB,Axelrod B

doi

10.1104/pp.59.4.695

subject

Has Abstract

pub_date

1977-04-01 00:00:00

pages

695-700

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

59

pub_type

杂志文章
  • Comparative Expression Profiling Reveals Genes Involved in Megasporogenesis.

    abstract::Megasporogenesis is a key step during ovule development in angiosperms, but the small number and inaccessibility of these cells have hampered molecular and genome-wide studies. Thus, many questions remain regarding the molecular basis of cell specification, differentiation, and development in the female gametophyte. H...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01254

    authors: Zhao H,Guo M,Yan M,Cheng H,Liu Y,She Z,Lai L,Shi C,Zhang M,Li Y,Lin D,Qin Y

    更新日期:2020-04-01 00:00:00

  • Estimating photosynthesis and concurrent export rates in C3 and C4 species at ambient and elevated CO21,2

    abstract::The ability of 21 C3 and C4 monocot and dicot species to rapidly export newly fixed C in the light at both ambient and enriched CO2 levels was compared. Photosynthesis and concurrent export rates were estimated during isotopic equilibrium of the transport sugars using a steady-state 14CO2-labeling procedure. At ambien...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.1.207

    authors: Grodzinski B,Jiao J,Leonardos ED

    更新日期:1998-05-01 00:00:00

  • Site-specific nitrosoproteomic identification of endogenously S-nitrosylated proteins in Arabidopsis.

    abstract::Nitric oxide (NO) regulates multiple developmental events and stress responses in plants. A major biologically active species of NO is S-nitrosoglutathione (GSNO), which is irreversibly degraded by GSNO reductase (GSNOR). The major physiological effect of NO is protein S-nitrosylation, a redox-based posttranslational ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00026

    authors: Hu J,Huang X,Chen L,Sun X,Lu C,Zhang L,Wang Y,Zuo J

    更新日期:2015-04-01 00:00:00

  • CYP79D73 Participates in Biosynthesis of Floral Scent Compound 2-Phenylethanol in Plumeria rubra.

    abstract::Plumeria (Plumeria rubra), well known for its brightly colored and fragrant flowers, emits a number of floral volatile organic compounds (VOCs). Plumeria flowers emit a total of 43 VOCs including nine phenylpropanoids/benzenoids, such as 2-phenylethanol (2PE), benzaldehyde, 2-phenylacetaldehyde (PAld), (E/Z)-phenylace...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00098

    authors: Dhandapani S,Jin J,Sridhar V,Chua NH,Jang IC

    更新日期:2019-05-01 00:00:00

  • Actin-bundling protein isolated from pollen tubes of lily. Biochemical and immunocytochemical characterization

    abstract::A 135-kD actin-bundling protein was purified from pollen tubes of lily (Lilium longiflorum) using its affinity to F-actin. From a crude extract of the pollen tubes, this protein was coprecipitated with exogenously added F-actin and then dissociated from F-actin by treating it with high-ionic-strength solution. The pro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.4.1421

    authors: Yokota E,Shimmen KiTT

    更新日期:1998-04-01 00:00:00

  • Complex I impairment, respiratory compensations, and photosynthetic decrease in nuclear and mitochondrial male sterile mutants of Nicotiana sylvestris.

    abstract::We have previously shown that in Nicotiana sylvestris cytoplasmic male-sterile (CMS) mutants where the mtDNA lacks the nad7 gene coding for a subunit of respiratory Complex I (NADH:ubiquinone oxidoreductase, EC 1.6.5.3), glycine (Gly) oxidation was lower than in the wild type and insensitive to rotenone, suggesting Co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.3.1239

    authors: Sabar M,De Paepe R,de Kouchkovsky Y

    更新日期:2000-11-01 00:00:00

  • delta-Aminolevulinic Acid Synthase of Euglena gracilis: Regulation of Activity.

    abstract::delta-Aminolevulinic acid (ALA), a key precursor of the tetrapyrroles heme and chlorophyll, is capable of being synthesized by two different routes in cells of the unicellular green alga Euglena gracilis: from the intact carbon skeleton of glutamate, and via the condensation of glycine and succinyl CoA, mediated by th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.1.219

    authors: Foley T,Dzelzkalns V,Beale SI

    更新日期:1982-07-01 00:00:00

  • Rotational streaming in fiber cells and its role in translocation.

    abstract::All visible protoplasmic streaming in sections of various plant stems was reversibly stopped by 2,4-dinitrophenol (DNP). Sections contained epidermal, cortical, and fiber cell types. Cells treated with DNP retained their semipermeability as evidenced by their plasmolysis in sucrose solutions. Washing out the DNP resul...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.10.1648

    authors: Worley JF

    更新日期:1968-10-01 00:00:00

  • Reduction of Iron by Leaf Extracts and Its Significance for the Assay of Fe(II) Iron in Plants.

    abstract::"Active Fe" in plants has generally been interpreted to be ferrous iron. This study evaluated some of the commonly followed active Fe determination procedures based on Fe(II) measurements. In each of 12 species examined, leaf extracts exhibited strong reducing activity and quickly reduced any added Fe(III) with or wit...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.3.1017

    authors: Mehrotra SC,Gupta P

    更新日期:1990-07-01 00:00:00

  • An evaluation of 2,5-norbornadiene as a reversible inhibitor of ethylene action in deepwater rice.

    abstract::Partial submergence of deepwater rice (Oryza sativa L. cv Habiganj Aman II) elicits three responses: enhancement of internodal elongation, inhibition of leaf growth, and promotion of adventitious root formation. All three responses can be induced in isolated stem sections by treatment with ethylene. Dose-response curv...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.2.395

    authors: Bleecker AB,Rose-John S,Kende H

    更新日期:1987-06-01 00:00:00

  • Interactions of Avocado (Persea americana) Cytochrome P-450 with Monoterpenoids.

    abstract::The microsomal fraction of avocado (Persea americana) mesocarp is a rich source of cytochrome P-450 active in the demethylation of xenobiotics. Cytochrome P-450 from this tissue has been purified and well characterized at the molecular level (DP O'Keefe, KJ Leto [1989] Plant Physiol 89: 1141-1149; KR Bozak, H Yu, R Si...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.4.1290

    authors: Hallahan DL,Nugent JH,Hallahan BJ,Dawson GW,Smiley DW,West JM,Wallsgrove RM

    更新日期:1992-04-01 00:00:00

  • Down-regulation of caffeic acid o-methyltransferase in maize revisited using a transgenic approach.

    abstract::Transgenic maize (Zea mays) plants were generated with a construct harboring a maize caffeic acid O-methyltransferase (COMT) cDNA in the antisense (AS) orientation under the control of the maize Adh1 (alcohol dehydrogenase) promoter. Adh1-driven beta-glucuronidase expression was localized in vascular tissues and ligni...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.012237

    authors: Piquemal J,Chamayou S,Nadaud I,Beckert M,Barrière Y,Mila I,Lapierre C,Rigau J,Puigdomenech P,Jauneau A,Digonnet C,Boudet AM,Goffner D,Pichon M

    更新日期:2002-12-01 00:00:00

  • Further characterization of the magnesium chelatase in isolated developing cucumber chloroplasts : substrate specificity, regulation, intactness, and ATP requirements.

    abstract::Mg-chelatase catalyzes the first step unique to the chlorophyll branch of tetrapyrrole biosynthesis, namely the insertion of Mg into protoporphyrin IX (Proto). Mg-chelatase was assayed in intact chloroplasts from semi-green cucumber (Cucumis sativus, cv Sumter) cotyledons. In the presence of Proto and MgATP, enzyme ac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.4.1189

    authors: Walker CJ,Weinstein JD

    更新日期:1991-04-01 00:00:00

  • Solution-Flow in the Phloem: II. Phloem Transport of THO in Beta vulgaris.

    abstract::Translocation profiles along the path were studied using a modified flap-feeding technique for the simultaneous application of THO and (14)C-sucrose. A re-evaluation of a mathematical model for phloem transport with reversible lateral exchange of tracer along the path indicates that lower apparent velocities for THO a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.5.690

    authors: Cataldo DA,Christy AL,Coulson CL

    更新日期:1972-05-01 00:00:00

  • Differential expression of the TFIIIA regulatory pathway in response to salt stress between Medicago truncatula genotypes.

    abstract::Soil salinity is one of the most significant abiotic stresses for crop plants, including legumes. These plants can establish root symbioses with nitrogen-fixing soil bacteria and are able to grow in nitrogen-poor soils. Medicago truncatula varieties show diverse adaptive responses to environmental conditions, such as ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.106146

    authors: de Lorenzo L,Merchan F,Blanchet S,Megías M,Frugier F,Crespi M,Sousa C

    更新日期:2007-12-01 00:00:00

  • Characteristics of ethylene biosynthesis-inducing xylanase movement in tobacco leaves.

    abstract::(125)I-Labeled ethylene biosynthesis-inducing xylanase (EIX) was used to study the movement of this protein in tobacco (Nicotiana tabacum) tissues. A biologically active (125)I-labeled EIX was obtained using chloramine-T as the oxidizing agent. Labeled EIX was detected in the far most edges of the leaf 5 min after it ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.4.2059

    authors: Sharon A,Bailey BA,McMurtry JP,Taylor R,Anderson JD

    更新日期:1992-12-01 00:00:00

  • Cytokinin-induced wall extensibility in excised cotyledons of radish and cucumber.

    abstract::The mechanism of cytokinin-induced cell expansion in cotyledons excised from dark-grown seedlings of radish (Raphanus sativus L.) and cucumber (Cucumus sativus L.) was studied. Cotyledons were incubated in dim light with or without 17 micromolar zeatin for periods up to 3 days. Fresh weights and osmotic potentials wer...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.1.107

    authors: Thomas J,Ross CW,Chastain CJ,Koomanoff N,Hendrix JE

    更新日期:1981-07-01 00:00:00

  • Combining quantitative trait Loci analysis and an ecophysiological model to analyze the genetic variability of the responses of maize leaf growth to temperature and water deficit.

    abstract::Ecophysiological models predict quantitative traits of one genotype in any environment, whereas quantitative trait locus (QTL) models predict the contribution of alleles to quantitative traits under a limited number of environments. We have combined both approaches by dissecting into effects of QTLs the parameters of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.013839

    authors: Reymond M,Muller B,Leonardi A,Charcosset A,Tardieu F

    更新日期:2003-02-01 00:00:00

  • Early steps in proanthocyanidin biosynthesis in the model legume Medicago truncatula.

    abstract::Oligomeric proanthocyanidins (PAs) composed primarily of epicatechin units accumulate in the seed coats of the model legume Medicago truncatula, reaching maximal levels at around 20 d after pollination. Genes encoding the single Medicago anthocyanidin synthase (ANS; EC 1.14.11.19) and leucoanthocyanidin reductase (LAR...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.107326

    authors: Pang Y,Peel GJ,Wright E,Wang Z,Dixon RA

    更新日期:2007-11-01 00:00:00

  • Decreased mitochondrial activities of malate dehydrogenase and fumarase in tomato lead to altered root growth and architecture via diverse mechanisms.

    abstract::Transgenic tomato (Solanum lycopersicum) plants in which either mitochondrial malate dehydrogenase or fumarase was antisense inhibited have previously been characterized to exhibit altered photosynthetic metabolism. Here, we demonstrate that these manipulations also resulted in differences in root growth, with both tr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.130518

    authors: van der Merwe MJ,Osorio S,Moritz T,Nunes-Nesi A,Fernie AR

    更新日期:2009-02-01 00:00:00

  • Evaluation of h secretion relative to zeatin-induced growth of detached cucumber cotyledons.

    abstract::Cytokinins promote expansion of cotyledons detached from seedlings of more than a dozen species. The zeatin-enhanced expansion of cucumber (Cucumis sativus L. cv Marketer) cotyledons was investigated. In addition, whether acid secretion is involved in wall loosening accompanying such accelerated growth was evaluated. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.5.1470

    authors: Ross CW,Rayle DL

    更新日期:1982-11-01 00:00:00

  • Chlorophyll a fluorescence and carbon assimilation in developing leaves of light-grown cucumber.

    abstract::The development of photochemical activity and carbon assimilation in light-grown cucumber (Cucumis sativus L. cv Natsusairaku) leaves was studied to determine the pattern of acquisition and its relationship to leaf growth and expansion. Measurements of chlorophyll a fluorescence showed that leaves acquire photochemica...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.3.1078

    authors: Croxdale JG,Omasa K

    更新日期:1990-07-01 00:00:00

  • Substitution of redox chemicals for radiation in phytochrome-mediated photomorphogenesis.

    abstract::The reducing agents, potassium ferrocyanide, beta-mercaptoethylamine, cysteine, reduced DPN, ferrous sulfate, methyl viologen and ascorbic acid caused the expansion in darkness of disks of primary leaf tissue cut from dark-grown bean plants. The reducing agents interacted synergistically with low irradiances of red ra...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.6.949

    authors: Klein RM,Edsall PC

    更新日期:1966-06-01 00:00:00

  • Effects of Salinity on Water Transport of Excised Maize (Zea mays L.) Roots.

    abstract::The root pressure probe was used to determine the effects of salinity on the hydraulic properties of primary roots of maize (Zea mays L. cv Halamish). Maize seedlings were grown in nutrient solutions modified by additions of NaCl and/or extra CaCl(2) so that the seedlings received one of four treatments: Control, plus...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.3.1136

    authors: Azaizeh H,Steudle E

    更新日期:1991-11-01 00:00:00

  • The alternative oxidase of plant mitochondria is involved in the acclimation of shoot growth at low temperature. A study of Arabidopsis AOX1a transgenic plants.

    abstract::The alternative oxidase (AOX) pathway of plant mitochondria uncouples respiration from mitochondrial ATP production and may ameliorate plant performance under stressful environmental conditions, such as cold temperatures, by preventing excess accumulation of reactive oxygen species. We tested this model in whole tissu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.070789

    authors: Fiorani F,Umbach AL,Siedow JN

    更新日期:2005-12-01 00:00:00

  • Cadmium uptake kinetics in intact soybean plants.

    abstract::The absorption characteristics of Cd(2+) by 10- to 12-day-old soybean plants (Glycine max cv Williams) were investigated with respect to influence of Cd concentration on adsorption to root surfaces, root absorption, transport kinetics and interaction with the nutrient cations Cu(2+), Fe(2+), Mn(2+), and Zn(2+). The fr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.3.844

    authors: Cataldo DA,Garland TR,Wildung RE

    更新日期:1983-11-01 00:00:00

  • In Vitro Prenylation of the Small GTPase Rac13 of Cotton.

    abstract::Previous work (D.P. Delmer, J. Pear, A. Andrawis, D. Stalker [1995] Mol Gen Genet 248: 43-51) has identified a gene in cotton (Gossypium hirsutum), Rac13, that encodes a small, signal-transducing GTPase and shows high expression in the fiber at the time of transition from primary to secondary wall synthesis. Since Rac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.4.1491

    authors: Trainin T,Shmuel M,Delmer DP

    更新日期:1996-12-01 00:00:00

  • Molecular characterization of oat seed globulins.

    abstract::We have isolated full-length cDNA clones that encode oat (Avena sativa) seed storage globulin mRNAs from a cDNA library in the expression vector lambda gtll. The longest of these clones, pOG2, has an 1840-base pair insert that encodes a complete precursor subunit with a signal peptide of 24 amino acids followed by an ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.3.698

    authors: Shotwell MA,Afonso C,Davies E,Chesnut RS,Larkins BA

    更新日期:1988-07-01 00:00:00

  • Role of bacterial lipopolysaccharide in attachment of agrobacterium to moss.

    abstract::Gametophore induction in moss by Agrobacterium tumefaciens was inhibited by addition of lipopolysaccharide (LPS) from A. tumefaciens. The LPS did not affect bacterial viability or appear to bind to bacterial cells. LPS from nonbinding Agrobacterium radiobacter was not effective in reducing gametophore formation. A. tu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.5.765

    authors: Whatley MH,Spiess LD

    更新日期:1977-11-01 00:00:00

  • AtMetExpress development: a phytochemical atlas of Arabidopsis development.

    abstract::Plants possess many metabolic genes for the production of a wide variety of phytochemicals in a tissue-specific manner. However, the metabolic systems behind the diversity and tissue-dependent regulation still remain unknown due to incomplete characterization of phytochemicals produced in a single plant species. Thus,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.148031

    authors: Matsuda F,Hirai MY,Sasaki E,Akiyama K,Yonekura-Sakakibara K,Provart NJ,Sakurai T,Shimada Y,Saito K

    更新日期:2010-02-01 00:00:00