Abstract:
:Two isoenzymes of lipoxygenase, a and b, have been obtained from bush beans (Phaseolus vulgaris) as electrophoretically homogeneous proteins. Both proteins have a molecular weight of 100,000, contain 1 atom of iron, and appear to be composed of a single peptide chain. However, these enzymes appear to differ in some other respects. Thus, lipoxygenase a has an isoelectric point of 6.03 while lipoxygenase b has a value of 5.57. Their pH optima are 5 to 7 and 6.5 to 7, respectively. Both lipoxygenase a and b, when acting on linoleic acid plus the product hydroperoxide, generate what are presumably keto-dienes with an absorption maximum at 280 nm. Whereas lipoxygenase a can catalyze this secondary reaction in the presence of O(2), lipoxygenase b does so only under anaerobic conditions. Lipoxygenase a is stimulated by Ca(2+) while lipoxygenase is not. An unexpected finding is the strong inhibition of lipoxygenase a by Mn(2+) (50% inhibition at 12.5 muM under standard reaction conditions). Lipoxygenase b is inhibited by Mn(2+) but only at concentrations about 250 times greater.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Hurt GB,Axelrod Bdoi
10.1104/pp.59.4.695subject
Has Abstractpub_date
1977-04-01 00:00:00pages
695-700issue
4eissn
0032-0889issn
1532-2548journal_volume
59pub_type
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