Abstract:
:Plumeria (Plumeria rubra), well known for its brightly colored and fragrant flowers, emits a number of floral volatile organic compounds (VOCs). Plumeria flowers emit a total of 43 VOCs including nine phenylpropanoids/benzenoids, such as 2-phenylethanol (2PE), benzaldehyde, 2-phenylacetaldehyde (PAld), (E/Z)-phenylacetaldoxime (PAOx), benzyl nitrile (BN), and 2-phenylnitroethane (PN). To identify genes and pathways involved in the production of the major compound 2PE, we analyzed the plumeria floral transcriptome and found a highly expressed, flower-specific gene encoding a cytochrome P450 family 79D protein (PrCYP79D73), which catalyzed the formation of (E/Z)-PAOx. Feeding experiments with deuterated phenylalanine or deuterated (E/Z)-PAOx showed that (E/Z)-PAOx is an intermediate in the biosynthesis of 2PE, as are two nitrogen-containing volatiles, BN and PN, in plumeria flowers. Crude enzyme extracts from plumeria flowers converted l-phenylalanine to (E/Z)-PAOx, PAld, 2PE, BN, and PN. The biosynthesis of these compounds increased with addition of PrCYP79D73-enriched microsomes but was blocked by pretreatment with 4-phenylimidazole, an inhibitor of cytochrome P450 enzymes. Moreover, overexpression of PrCYP79D73 in Nicotiana benthamiana resulted in the emission of (E/Z)-PAOx as well as PAld, 2PE, BN, and PN, all of which were also found among plumeria floral VOCs. Taken together, our results demonstrate that PrCYP79D73 is a crucial player in the biosynthesis of the major floral VOC 2PE and other nitrogen-containing volatiles. These volatiles may be required for plant defense as well as to attract pollinators for the successful reproduction of plumeria.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Dhandapani S,Jin J,Sridhar V,Chua NH,Jang ICdoi
10.1104/pp.19.00098subject
Has Abstractpub_date
2019-05-01 00:00:00pages
171-184issue
1eissn
0032-0889issn
1532-2548pii
pp.19.00098journal_volume
180pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Glutathione reductase (EC 1.6.4.2) activity is present in spinach (Spinacia oleracea L.) chloroplasts. The pH dependence and substrate concentration for half-maximal rate are reported and a possible role in chloroplasts is proposed. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.59.5.1011
更新日期:1977-05-01 00:00:00
abstract::The plant hormone jasmonic acid (JA) plays a pivotal role in plant-insect interactions. Herbivore attack usually elicits dramatic increases in JA concentrations, which in turn activate the accumulation of metabolites that function as defenses against herbivores. Although almost all enzymes involved in the biosynthesis...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.199018
更新日期:2012-08-01 00:00:00
abstract::The changes in root system architecture (RSA) triggered by phosphate (P) deprivation were studied in Arabidopsis (Arabidopsis thaliana) plants grown for 14 d on 1 mM or 3 microM P. Two different temporal phases were observed in the response of RSA to low P. First, lateral root (LR) development was promoted between day...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.060061
更新日期:2005-08-01 00:00:00
abstract::The phytochrome (phy) family of sensory photoreceptors (phyA-phyE in Arabidopsis thaliana) induces changes in target-gene expression upon light-induced translocation to the nucleus, where certain members interact with selected members of the constitutively nuclear basic helix-loop-helix transcription factor family, su...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.105601
更新日期:2007-11-01 00:00:00
abstract::The action spectrum of apparent photosynthesis for attached radish (Raphanus sativus L. var. Early Scarlet Globe) and corn (Zea mays L. var. Pride V.) leaves was measured at 300 mul/l CO(2) and both 21% and 2% O(2). The spectra were measured at light intensities where apparent photosynthesis was proportional to intens...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.5.678
更新日期:1969-05-01 00:00:00
abstract::Glutathione is generally accepted as the principal electron donor for dehydroascorbate (DHA) reduction. Moreover, both glutathione and DHA affect cell cycle progression in plant cells. But other mechanisms for DHA reduction have been proposed. To investigate the connection between DHA and glutathione, we have evaluate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.033548
更新日期:2004-04-01 00:00:00
abstract::The effects of oxidative stress on the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1.39) were studied in isolated chloroplasts from barley (Hordeum vulgare L. cv Angora). Active oxygen (AO) was generated by varying the light intensity, the oxygen concentration, or the addition of he...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.3.789
更新日期:1996-07-01 00:00:00
abstract::A beta-primeverosidase from tea (Camellia sinensis) plants is a unique disaccharide-specific glycosidase, which hydrolyzes aroma precursors of beta-primeverosides (6-O-beta-D-xylopyranosyl-beta-D-glucopyranosides) to liberate various aroma compounds, and the enzyme is deeply concerned with the floral aroma formation i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.011023
更新日期:2002-12-01 00:00:00
abstract::The plant hormone abscisic acid (ABA) is involved in an array of plant processes, including the regulation of gene expression during adaptive responses to various environmental cues. Apart from its well-established role in abiotic stress adaptation, emerging evidence indicates that ABA is also prominently involved in ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.152702
更新日期:2010-04-01 00:00:00
abstract::Suboptimal N or P availability and cool temperatures all decrease apparent hydraulic conductance (L) of cotton (Gossypium hirsutum L.) roots. The interaction between nutrient status and root temperature was tested in seedlings grown in nutrient solutions. The depression of L (calculated as the ratio of transpiration r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.92.3.855
更新日期:1990-03-01 00:00:00
abstract::The essential oils of peppermint (Mentha x piperita) and spearmint (Mentha spicata) are distinguished by the oxygenation position on the p-menthane ring of the constitutive monoterpenes that is conferred by two regiospecific cytochrome P450 limonene-3- and limonene-6-hydroxylases. Following hydroxylation of limonene, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.053298
更新日期:2005-03-01 00:00:00
abstract::Changes in the galactose content of the noncellulosic polysaccharides of tomato (Mill) fruit cell walls were analyzed under various conditions. On the plant, galactan decreased gradually during fruit growth. As normal fruits ripened, the loss of galactan increased sharply; this was not observed in attached rin fruits ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.3.532
更新日期:1980-09-01 00:00:00
abstract::Chlamydomonas reinhardtii adapts to the stress of CO(2)-limiting conditions through the induction of a set of genes including CAH1, which encodes a periplasmic carbonic anhydrase. CAH1 is up-regulated under low-CO(2) conditions (air containing 0.04% [v/v] CO(2)) in the presence of light, whereas it is down-regulated u...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.4.1329
更新日期:1999-12-01 00:00:00
abstract::A mutant strain of Chlamydomonas reinhardtii, designated 18-7F, has been isolated and characterized. 18-7F requires a high CO(2) concentration for photoautrophic growth in spite of the apparent induction of a functional CO(2) concentrating mechanism in air-adapted cells. In 2% O(2) the photosynthetic characteristics o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.1.231
更新日期:1990-05-01 00:00:00
abstract::Allene oxide synthase (AOS; hydroperoxide dehydratase; EC 4.2.1.92) catalyzes the first step in the biosynthesis of jasmonic acid from lipoxygenase-derived hydroperoxides of free fatty acids. Using the AOS cDNA from tomato (Lycopersicon esculentum), in which the role of jasmonic acid in wound-induced defense gene acti...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.4.1335
更新日期:2000-04-01 00:00:00
abstract::Prevention of actin polymerization with low concentrations of latrunculin B (Lat-B; 2 nm) exerts a profound inhibitory effect on pollen tube growth. Using flow-through chambers, we show that growth retardation starts after 10 min treatment with 2 nm Lat-B, and by 15 to 20 min reaches a basal rate of 0.1 to 0.2 microm/...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.113035
更新日期:2008-04-01 00:00:00
abstract::The LOSS OF APOMEIOSIS (LOA) locus is one of two dominant loci known to control apomixis in the eudicot Hieracium praealtum. LOA stimulates the differentiation of somatic aposporous initial cells after the initiation of meiosis in ovules. Aposporous initial cells undergo nuclear proliferation close to sexual megaspore...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.181164
更新日期:2011-11-01 00:00:00
abstract::We report the molecular characterization and functional analysis of a gene (PsdeltaOAT) from Scots pine (Pinus sylvestris) encoding Orn-delta-aminotransferase (delta-OAT; EC 2.6.1.13), an enzyme of arginine metabolism. The deduced amino acid sequence contains a putative N-terminal signal peptide for mitochondrial targ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.122853
更新日期:2008-09-01 00:00:00
abstract::In black cherry (Prunus serotina Ehrh.) homogenates, (R)-amygdalin is catabolized to HCN, benzaldehyde, and d-glucose by the sequential action of amygdalin hydrolase, prunasin hydrolase, and mandelonitrile lyase. The tissue and subcellular localizations of these enzymes were determined within intact black cherry seeds...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.1.291
更新日期:1992-09-01 00:00:00
abstract::The effect of ultraviolet B (UV-B) radiation on the thermal sensitivity of cucumber (Cucumis sativus L.) was studied using UV-B-sensitive cv Poinsett 76 and UV-B-resistant cv Ashley grown under control and elevated (300 mW m-2) UV-B radiation levels. Using both cotyledon and leaf discs, the ability of the tissue to re...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.2.395
更新日期:1994-02-01 00:00:00
abstract::Lemna paucicostata Hegelm. 6746 possesses specific systems for uptake of choline and ethanolamine. Each is distinct from the six other systems for uptake of organic compounds so far identified in this plant. Both systems show biphasic kinetics, so that uptake by them can be described as the composite result of two Mic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.1.285
更新日期:1986-05-01 00:00:00
abstract::Many plants flower in response to seasonal changes in daylength. This response often varies between accessions of a single species. We studied the variation in photoperiod response found in the model species Arabidopsis (Arabidopsis thaliana). Seventy-two accessions were grown under six daylengths varying in 2-h inter...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.140772
更新日期:2010-01-01 00:00:00
abstract::Plant cystatins, similar to other defense proteins, include hypervariable, positively selected amino acid sites presumably impacting their biological activity. Using 29 single mutants of the eighth domain of tomato (Solanum lycopersicum) multicystatin, SlCYS8, we assessed here the potential of site-directed mutagenesi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.115741
更新日期:2008-03-01 00:00:00
abstract::The biosynthetic pathway to n-heptane was investigated by examining the effect of the [beta]-keto acyl-acyl carrier protein synthase inhibitor (2R,3S)-2,3-epoxy-4-oxo-7E,10E-dodecadienamide (cerulenin), a thiol reagent ([beta]-mercaptoethanol), and an aldehydetrapping reagent (hydroxylamine) on the biosynthesis of n-[...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.4.1263
更新日期:1996-08-01 00:00:00
abstract::Extracts of the tendrils of Pisum sativum, Var. Alaska, exhibit adenosine triphosphatase activity which is inversely proportional to the amount the tendrils have coiled. The specific viscosity of the extract decreases when ATP is added. This evidence indicates a possible role of a contractile adenosine triphosphatase ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.6.845
更新日期:1967-06-01 00:00:00
abstract::Computing techniques are used to simulate the course of uptake of K(+), Na(+), and Cl(-) by low-salt roots. Measurements of the fluxes of these ions in high-salt roots are used to calculate membrane permeabilities, which are then used to calculate cell uptake. In this way it is possible to test the predictive value of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.10.1417
更新日期:1969-10-01 00:00:00
abstract::Mitochondria from cotyledons of Vigna sesquipedalis (L.) Fruwirth (starchy seed) showed no NAD-isocitric dehydrogenase (NAD-IDH) activity by the methods which have been known to be useful for the detection of NAD-IDH in mitochondria of plants including castor bean and alaska pea. When the Vigna cotyledon mitochondria ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.2.262
更新日期:1969-02-01 00:00:00
abstract::The growth of lateral buds (tillers), which are undergoing release from apical dominance, was measured in upright and gravistimulated intact Avena sativa L. cv. ;Victory' (oat) shoots as well as in isolated Avena stem segments treated with kinetin and sucrose. During release, the tiller bud initially shows a slow rate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.3.811
更新日期:1982-09-01 00:00:00
abstract::Partitioning and translocation of (14)C-photosynthates were examined during flowering and seed maturation in soybean (Glycine max [L.]Merr.) plants to quantify allocation to sugars, amino acids, organic acids, and starch and to study transport of C and N from leaves to reproductive sinks. The trifoliolate leaf at the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.4.1040
更新日期:1984-08-01 00:00:00
abstract::The patterns of nitrate reductase activity (NRA) in the leaves (in vivo assay) and root nodule nitrogenase activity (C(2)H(2) reduction) were investigated throughout the season in field-grown Phaseolus vulgaris plants.Maximal NRA (per g fresh weight) occurred at early stages of leaf development but total activity (per...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.3.421
更新日期:1979-03-01 00:00:00