Abstract:
:The essential oils of peppermint (Mentha x piperita) and spearmint (Mentha spicata) are distinguished by the oxygenation position on the p-menthane ring of the constitutive monoterpenes that is conferred by two regiospecific cytochrome P450 limonene-3- and limonene-6-hydroxylases. Following hydroxylation of limonene, an apparently similar dehydrogenase oxidizes (-)-trans-isopiperitenol to (-)-isopiperitenone in peppermint and (-)-trans-carveol to (-)-carvone in spearmint. Random sequencing of a peppermint oil gland secretory cell cDNA library revealed a large number of clones that specified redox-type enzymes, including dehydrogenases. Full-length dehydrogenase clones were screened by functional expression in Escherichia coli using a recently developed in situ assay. A single full-length acquisition encoding (-)-trans-isopiperitenol dehydrogenase (ISPD) was isolated. The (-)-ISPD cDNA has an open reading frame of 795 bp that encodes a 265-residue enzyme with a calculated molecular mass of 27,191. Nondegenerate primers were designed based on the (-)-trans-ISPD cDNA sequence and employed to screen a spearmint oil gland secretory cell cDNA library from which a 5'-truncated cDNA encoding the spearmint homolog, (-)-trans-carveol-dehydrogenase, was isolated. Reverse transcription-PCR amplification and RACE were used to acquire the remaining 5'-sequence from RNA isolated from oil gland secretory cells of spearmint leaf. The full-length spearmint dehydrogenase shares >99% amino acid identity with its peppermint homolog and both dehydrogenases are capable of utilizing (-)-trans-isopiperitenol and (-)-trans-carveol. These isopiperitenol/carveol dehydrogenases are members of the short-chain dehydrogenase/reductase superfamily and are related to other plant short-chain dehydrogenases/reductases involved in secondary metabolism (lignan biosynthesis), stress responses, and phytosteroid biosynthesis, but they are quite dissimilar (approximately 13% identity) to the monoterpene reductases of mint involved in (-)-menthol biosynthesis. The isolation of the genes specifying redox enzymes of monoterpene biosynthesis in mint indicates that these genes arose from different ancestors and not by simple duplication and differentiation of a common progenitor, as might have been anticipated based on the common reaction chemistry and structural similarity of the substrate monoterpenes.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Ringer KL,Davis EM,Croteau Rdoi
10.1104/pp.104.053298subject
Has Abstractpub_date
2005-03-01 00:00:00pages
863-72issue
3eissn
0032-0889issn
1532-2548pii
pp.104.053298journal_volume
137pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Plasma membrane H+-ATPase was immunolocalized in several cell types of the sensitive plant Mimosa pudica L., and transmembrane potentials were measured on cortical cells. In comparison with the nonspecialized cortical cells of the petiole or stem, the proton pump was highly expressed in motor cells. These immunologica...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.3.747
更新日期:1997-03-01 00:00:00
abstract::The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared proteomes of tissue cultures from leaf explants of these two lines. Both 2HA and Jemalong explants were grown on media containing the auxin 1-naphthaleneac...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.055277
更新日期:2005-04-01 00:00:00
abstract::No cytokinin activity was found in methanolic mesocarp extracts after purification with petroleum ether and ethyl acetate. Cytokinin activity did appear, however, in aqueous fraction after acid hydrolysis or passage through Dowex 50 (H(+)) ion exchange columns. The level of this activity, the fruit growth rate, and th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.46.2.334
更新日期:1970-08-01 00:00:00
abstract::Tubers of Solanum tuberosum L. var. Russet Burbank, Kennebec, and Targhee were stored at 95% relative humidity and at 1.7, 4.4, 5.8, 7.2 or 10 C. Rates of O(2) consumption were measured periodically by removing individual tubers from storage, excising discs of pith parenchyma tissue, and incubating the discs in a Gils...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.2.252
更新日期:1978-02-01 00:00:00
abstract::Stilbenes are considered the most important phytoalexin group in grapevine (Vitis vinifera) and they are known to contribute to the protection against various pathogens. The main stilbenes in grapevine are resveratrol and its derivatives and, among these, pterostilbene has recently attracted much attention due both to...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.126003
更新日期:2008-11-01 00:00:00
abstract::Amitrole (3-amino-s-triazole), dichlormate (3,4-dichlorobenzyl methylcarbamate), and pyriclor (2,3,5-trichloro-4-pyridinol) inhibited normal carotenogenesis in etiolated wheat (Triticum aestivum L. var. Coker 65-20) seedlings. Carotenoid precursors accumulated in treated plants. In dichlormate-treated plants, zeta-car...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.47.1.144
更新日期:1971-01-01 00:00:00
abstract::Rhizobitoxine, an inhibitor of methionine biosynthesis in Salmonella typhimurium, inhibited ethylene production about 75% in light-grown sorghum seedlings and in senescent apple tissue. Ethylene production stimulated by indoleacetic acid and kinetin in sorghum was similarly inhibited. With both apple and sorghum, the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.48.1.1
更新日期:1971-07-01 00:00:00
abstract::The intracellular distribution of the neurotoxin 2,4-diaminobutyric acid (DABA) in mature leaves of the perennial legume Lathyrus sylvestris L. var ;Lathco' (flatpea) was determined using subcellular fractions from mesophyll protoplasts. Chloroplasts contained about 15% of the cellular DABA. At least 75% of the DABA w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.4.900
更新日期:1987-04-01 00:00:00
abstract::Barley (Hordeum vulgare L. cv Halcyon) seedlings which had been grown in full strength complete inorganic nutrient media (containing 6 millimolar K(+)) had high internal K(+) concentrations and low values of K(+) ((86)Rb(+)) influx when influx was measured from solutions containing 100 micromolar K(+). Transfer of the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.92.4.1128
更新日期:1990-04-01 00:00:00
abstract::The inhibitory effect of NH4+ on net NO3- uptake has been attributed to an enhancement of efflux and, recently, to an inhibition of influx. To study this controversy, we devised treatments to distinguish the effects of NH4+ on these two processes. Roots of intact barley (Hordeum vulgare L.) seedlings, uninduced or ind...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.4.1293
更新日期:1994-12-01 00:00:00
abstract::The activities of several enzymes of the citric acid and poly-beta-hydroxybutyrate cycles were measured in Rhizobium japonicum 3I1B-143 bacteroids which had been isolated from soybean nodules by sucrose gradient centrifugation. During the period of developing nitrogenase activity, the specific activity of fumarase, hy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.4.1158
更新日期:1984-08-01 00:00:00
abstract::Isolated maize (Zea mays var. kelvedon glory) etioplasts have been used to investigate the relationships between the spectral shifts and ultrastructural changes which occur during light-induced chloroplast development. After primary photoconversion, the Shibata shift was observed as a change from 680 to 670 nm in the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.2.393
更新日期:1975-02-01 00:00:00
abstract::Fresh fruit and vegetables are a major source of ascorbic acid (vitamin C), an important antioxidant for the human diet and also for plants. Ascorbic acid content in fruit exhibits a quantitative inheritance. Quantitative trait loci (QTL) for ascorbic acid content have been mapped in three tomato populations derived f...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.091413
更新日期:2007-04-01 00:00:00
abstract::Fungal elicitor prepared from the cell walls of Aspergillum niger induces multiple responses of Hypericum perforatum cells, including nitric oxide (NO) generation, jasmonic acid (JA) biosynthesis, and hypericin production. To determine the role of NO and JA in elicitor-induced hypericin production, we study the effect...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.066407
更新日期:2005-10-01 00:00:00
abstract::Glyoxylate at a concentration of 10 millimolar caused 50% inhibition of decarboxylation of 20 millimolar [1-(14)C]glycine and accompanying synthesis of serine in a mitochondria-enriched preparation from tobacco (Nicotiana tabacum var. John Williams Broadleaf) leaves. None of the other compounds tested including format...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.1.61
更新日期:1982-07-01 00:00:00
abstract::It is demonstrated that, in etiolated pea (Pisum sativum) epicotyls, ethylene affects the activation of both monomeric GTP-binding proteins (monomeric G-proteins) and protein kinases. For monomeric G-proteins, the effect may be a rapid (2 min) and bimodal up-regulation, a transiently unimodal activation, or a transien...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.015057
更新日期:2003-04-01 00:00:00
abstract::It has been shown previously that an increase in ribulose diphosphate carboxylase activity occurs upon brief illumination of leaves of dark-grown Zea mays plants; an increase in ribose 5-phosphate isomerase occurs after prolonged illumination. Both of these responses to illumination are inhibited by chloramphenicol.Th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.2.188
更新日期:1968-02-01 00:00:00
abstract::Cool season crops face intermittent drought. Exposure to drought and other abiotic stresses is known to increase tolerance of the plants against subsequent exposure to such stresses. Storage of environmental signals is also proposed. Preexposure to a dehydration shock improved adaptive response during subsequent dehyd...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.043141
更新日期:2004-07-01 00:00:00
abstract::Phosphatidylcholine (PC) is a major membrane phospholipid and a precursor for major signaling molecules. Understanding its synthesis is important for improving plant growth, nutritional value, and resistance to stress. PC synthesis is complex, involving several interconnected pathways, one of which proceeds from serin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00457
更新日期:2018-08-01 00:00:00
abstract::Previous labeling experiments with (18)O(2) have supported the hypothesis that stress-induced abscisic acid (ABA) is synthesized through an indirect pathway involving an oxygenated carotenoid (xanthophyll) as a precursor. To investigate ABA formation under nonstress conditions, an (18)O(2) labeling experiment was cond...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.4.1039
更新日期:1989-04-01 00:00:00
abstract::Extracts of immature kernels of Zea mays L. catalyzed the synthesis of indol-3-yl-acetyl-myo-inositol arabinoside from indol-3-yl-acetyl-myo-inositol and UDP-[U-(14)C]xylose. The product contained radioactivity which upon hydrolysis with trifluoroacetic acid cochromatographed with arabinose and not xylose. The amount ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.6.1664
更新日期:1982-12-01 00:00:00
abstract::Production of disease symptoms represents the final phase of infectious diseases and is a main cause of crop loss and/or marketability. However, little is known about the molecular basis of disease symptom development. In this study, a genetic screening was conducted to identify Arabidopsis (Arabidopsis thaliana) muta...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.181826
更新日期:2011-12-01 00:00:00
abstract::The ricinine content of etiolated seedlings of Ricinus communis increased nearly 12-fold over a 4-day period. In plants quinolinic acid is an intermediate in the de novo pathway for the synthesis of pyridine nucleotides. The only known enzyme in the de novo pathway for pyridine nucleotide biosynthesis, quinolinic acid...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.4.603
更新日期:1974-04-01 00:00:00
abstract::Formation of the riboside-5'-monophosphate is a general feature of the metabolism of cytokinins in plants. As part of a study of the biological significance of the nucleotide form of cytokinins, we analyzed a mutant of Arabidopsis thaliana deficient in adenine phosphoribosyltransferase (APRT) activity for its ability ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.95.3.900
更新日期:1991-03-01 00:00:00
abstract::This work was undertaken in an effort to reconcile the conflicting proline-accumulating responses of the barley (Hordeum vulgare L.) cultivars, Excelsior and Proctor, reported by Singh et al. (1972) and Hanson et al. (1976). It deals with the effects of different vapor pressure deficits (VPD) during growth and subsequ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.2.716
更新日期:1992-02-01 00:00:00
abstract::Dual-conditional positive/negative selection markers are versatile genetic tools for manipulating genomes. Plastid genomes are relatively small and conserved DNA molecules that can be manipulated precisely by homologous recombination. High-yield expression of recombinant products and maternal inheritance of plastid-en...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.204107
更新日期:2012-12-01 00:00:00
abstract::An extract from 8-day-old cotton ovules (Gossypium hirsutum L.) was partitioned into three fractions and each fraction was derivatized and analyzed separately. Gas-liquid chromatography and computer-controlled gas-liquid chromatography-mass spectrometry were used to separate, measure, and identify the naturally occurr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.3.550
更新日期:1975-03-01 00:00:00
abstract::Microarray data can be used to derive understanding of the relationships between the genes involved in various biological systems of an organism, given the availability of databases of gene expression measurements from the complete spectrum of experimental conditions and materials. However, there have been no reports,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.139030
更新日期:2009-09-01 00:00:00
abstract::Biosynthesis of ent-kaurene was investigated in extracts of cell suspension cultures and seedlings of castor bean. Both cell-free extracts contain an inhibitor of kaurene synthetase. The inhibition affects mainly the cyclization of geranylgeranyl pyrophosphate to copalyl pyrophosphate (activity A) and has little or no...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.6.1169
更新日期:1981-06-01 00:00:00
abstract::Young seedlings of Lavatera cretica L. exhibit positive phototropism. The hypocotyl perceives unilateral illumination with blue light and curves towards the light source by unequal growth. In addition, the cotyledonary laminas perceive the vectorial component of unilateral illumination with blue light and reorient nor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.1.82
更新日期:1980-07-01 00:00:00