Intracellular Localization of the Neurotoxin 2,4-Diaminobutyric Acid in Lathyrus sylvestris L. Leaf Tissue.

Abstract:

:The intracellular distribution of the neurotoxin 2,4-diaminobutyric acid (DABA) in mature leaves of the perennial legume Lathyrus sylvestris L. var ;Lathco' (flatpea) was determined using subcellular fractions from mesophyll protoplasts. Chloroplasts contained about 15% of the cellular DABA. At least 75% of the DABA was vacuolar, based on the assumptions that each protoplast contained a single vacuole and that acid phosphatase occurred exclusively in the vacuole. DABA was not detectable in peroxisomal and mitochondrial fractions. Because the vacuole is not a major site of amino acid synthesis, this distribution implicates synthesis of DABA within chloroplasts with subsequent transport to and storage within the vacuoles of the mesophyll cells.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Foster JG,Cress WD,Wright SF,Hess JL

doi

10.1104/pp.83.4.900

subject

Has Abstract

pub_date

1987-04-01 00:00:00

pages

900-4

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

83

pub_type

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