Enzymes of the Poly-beta-Hydroxybutyrate and Citric Acid Cycles of Rhizobium japonicum Bacteroids.

Abstract:

:The activities of several enzymes of the citric acid and poly-beta-hydroxybutyrate cycles were measured in Rhizobium japonicum 3I1B-143 bacteroids which had been isolated from soybean nodules by sucrose gradient centrifugation. During the period of developing nitrogenase activity, the specific activity of fumarase, hydroxybutyrate dehydrogenase, beta-ketothiolase, and pyruvate dehydrogenase complex increased whereas acetoacetate-succinyl-CoA transferase and isocitrate dehydrogenase decreased. Malate dehydrogenase activity remained constant. The amount of available acetyl-CoA, based on pyruvate dehydrogenase activity, should be sufficient to support both metabolic cycles concurrently. The temporal relationship between nitrogenase activity and poly-beta-hydroxybutyrate accumulation has been reexamined.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Karr DB,Waters JK,Suzuki F,Emerich DW

doi

10.1104/pp.75.4.1158

subject

Has Abstract

pub_date

1984-08-01 00:00:00

pages

1158-62

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

75

pub_type

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