Molecular and functional analyses support a role of Ornithine-{delta}-aminotransferase in the provision of glutamate for glutamine biosynthesis during pine germination.

Abstract:

:We report the molecular characterization and functional analysis of a gene (PsdeltaOAT) from Scots pine (Pinus sylvestris) encoding Orn-delta-aminotransferase (delta-OAT; EC 2.6.1.13), an enzyme of arginine metabolism. The deduced amino acid sequence contains a putative N-terminal signal peptide for mitochondrial targeting. The polypeptide is similar to other delta-OATs from plants, yeast, and mammals and encoded by a single-copy gene in pine. PsdeltaOAT encodes a functional delta-OAT as determined by expression of the recombinant protein in Escherichia coli and analysis of the active enzyme. The expression of PsdeltaOAT was undetectable in the embryo, but highly induced at early stages of germination and seedling development in all different organs. Transcript levels decreased in later developmental stages, although an increase was observed in lignified stems of 90-d-old plants. An increase of delta-OAT activity was observed in germinating embryos and seedlings and appears to mirror the observed alterations in PsdeltaOAT transcript levels. Similar expression patterns were also observed for genes encoding arginase and isocitrate dehydrogenase. Transcripts of PsdeltaOAT and the arginase gene were found widely distributed in different cell types of pine organs. Consistent with these results a metabolic pathway is proposed for the nitrogen flow from the megagametophyte to the developing seedling, which is also supported by the relative abundance of free amino acids in embryos and seedlings. Taken together, our data support that delta-OAT plays an important role in this process providing glutamate for glutamine biosynthesis during early pine growth.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Cañas RA,Villalobos DP,Díaz-Moreno SM,Cánovas FM,Cantón FR

doi

10.1104/pp.108.122853

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

77-88

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.108.122853

journal_volume

148

pub_type

杂志文章
  • Cytokinin-induced wall extensibility in excised cotyledons of radish and cucumber.

    abstract::The mechanism of cytokinin-induced cell expansion in cotyledons excised from dark-grown seedlings of radish (Raphanus sativus L.) and cucumber (Cucumus sativus L.) was studied. Cotyledons were incubated in dim light with or without 17 micromolar zeatin for periods up to 3 days. Fresh weights and osmotic potentials wer...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.1.107

    authors: Thomas J,Ross CW,Chastain CJ,Koomanoff N,Hendrix JE

    更新日期:1981-07-01 00:00:00

  • Regulation of Pyrimidine Biosynthesis in Intact Cells of Cucurbita pepo.

    abstract::The occurrence of the complete orotic acid pathway for the biosynthesis de novo of pyrimidine nucleotides was demonstrated in the intact cells of roots excised from summer squash (Cucurbita pepo L. cv. Early Prolific Straightneck). Evidence that the biosynthesis of pyrimidine nucleotides proceeds via the orotate pathw...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.4.562

    authors: Lovatt CJ,Albert LS

    更新日期:1979-10-01 00:00:00

  • SUPPRESSOR OF FRIGIDA (SUF4) Supports Gamete Fusion via Regulating Arabidopsis EC1 Gene Expression.

    abstract::The EGG CELL1 (EC1) gene family of Arabidopsis (Arabidopsis thaliana) comprises five members that are specifically expressed in the egg cell and redundantly control gamete fusion during double fertilization. We investigated the activity of all five EC1 promoters in promoter-deletion studies and identified SUF4 (SUPPRE...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01024

    authors: Resentini F,Cyprys P,Steffen JG,Alter S,Morandini P,Mizzotti C,Lloyd A,Drews GN,Dresselhaus T,Colombo L,Sprunck S,Masiero S

    更新日期:2017-01-01 00:00:00

  • Large Cellular Inclusions Accumulate in Arabidopsis Roots Exposed to Low-Sulfur Conditions.

    abstract::Sulfur is vital for primary and secondary metabolism in plant roots. To understand the molecular and morphogenetic changes associated with loss of this key macronutrient, we grew Arabidopsis (Arabidopsis thaliana) seedlings in low-sulfur conditions. These conditions induced a cascade of cellular events that converged ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00465

    authors: Jackson TL,Baker GW,Wilks FR Jr,Popov VA,Mathur J,Benfey PN

    更新日期:2015-08-01 00:00:00

  • Germanium-68 as an adequate tracer for silicon transport in plants. Characterization of silicon uptake in different crop species.

    abstract::A basic problem in silicon (Si) uptake studies in biology is the lack of an appropriate radioactive isotope. Radioactive germanium-68 ((68)Ge) has been used previously as a Si tracer in biological materials, but its suitability for the study of Si transport in higher plants is still untested. In this study, we investi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.090845

    authors: Nikolic M,Nikolic N,Liang Y,Kirkby EA,Römheld V

    更新日期:2007-01-01 00:00:00

  • Combining quantitative trait Loci analysis and an ecophysiological model to analyze the genetic variability of the responses of maize leaf growth to temperature and water deficit.

    abstract::Ecophysiological models predict quantitative traits of one genotype in any environment, whereas quantitative trait locus (QTL) models predict the contribution of alleles to quantitative traits under a limited number of environments. We have combined both approaches by dissecting into effects of QTLs the parameters of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.013839

    authors: Reymond M,Muller B,Leonardi A,Charcosset A,Tardieu F

    更新日期:2003-02-01 00:00:00

  • Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation.

    abstract::Plant architecture is determined by genetic and developmental programs as well as by environmental factors. Sessile plants have evolved a subtle adaptive mechanism that allows them to alter their growth and development during periods of stress. Phytohormones play a central role in this process; however, the molecules ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.146803

    authors: Zhang SW,Li CH,Cao J,Zhang YC,Zhang SQ,Xia YF,Sun DY,Sun Y

    更新日期:2009-12-01 00:00:00

  • An Explanation for the Difference in Photosynthetic Capabilities of Healthy and Beet Yellows Virus-infected Sugar Beets (Beta vulgaris L.).

    abstract::Sugar beets (Beta vulgaris L.) infected with the Beet Yellows Virus exhibit lower rates of net photosynthesis at light saturation than do healthy plants. These Pn reductions were correlated with increases in leaf resistance to water vapor loss. Theoretical analyses demonstrated that, although the leaf resistance to wa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.5.576

    authors: Hall AE,Loomis RS

    更新日期:1972-11-01 00:00:00

  • Structure and function of developing barley plastids.

    abstract::Five different regions of the first foliage leaf of etiolated barley seedlings were studied with respect to leaf growth, plastid growth and replication, differentiation of etioplasts, and conversion of etioplasts into chloroplasts upon illumination. Ultrastructural changes of the plastids were correlated with chloroph...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.2.148

    authors: Robertson D,Laetsch WM

    更新日期:1974-08-01 00:00:00

  • Interference in Carotenogenesis as a Mechanism of Action of the Pyridazinone Herbicide Sandoz 6706: Accumulation of C-40 Carotenoid Precursors Inhibition of beta-Carotene Synthesis and Enhancement of Phytoene Epoxidation.

    abstract::The herbicide Sandoz 6706 (4-chloro-5-(dimethylamino)-2-alpha,alpha,alpha, (trifluoro-m-tolyl)-3(2H)-pyridazinone), when applied as a preplant soil treatment at a concentration of 0.05 mug/g reduced the content of beta-carotene and chlorophylls in 21-day-old wheat seedlings (Triticum aestivum L.) by 55% and 29%, respe...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.6.916

    authors: Ben-Aziz A,Koren E

    更新日期:1974-12-01 00:00:00

  • Effects of decenylsuccinic Acid on p uptake and translocation by barley and winter wheat.

    abstract::After 3 days of exposure to 10(-3) and 10(-4) M decenylsuccinic acid, winter wheat plants wilted and died. Decenylsuccinate at 10(-3) M inhibited (32)P uptake by barley roots and wheat roots and resulted in significant (P

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.45.1.1

    authors: Green DG,Ferguson WS,Warder FG

    更新日期:1970-01-01 00:00:00

  • Abnormal Stomatal Behavior and Hormonal Imbalance in flacca, a Wilty Mutant of Tomato: IV. Effect of Abscisic Acid and Water Content on RNase Activity and RNA.

    abstract::Plants of the wilty tomato (Lycopersicum esculentum) mutant, flacca, and of the normal cultivar Rheinlands Ruhm growing under either "normal" or high humidity were used in this research. Under normal humidity, RNase activity was much higher in mutant plants in which abscisic acid (ABA) and water content were lower tha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.2.173

    authors: Puri J,Tal M

    更新日期:1977-02-01 00:00:00

  • Modification of Sulfhydryl Groups in the [gamma]-Subunit of Chloroplast-Coupling Factor 1 Affects the Proton Slip through the ATP Synthase.

    abstract::A large proton leak not coupled to ATP synthesis (slip) occurs at alkaline pH through the chloroplast ATP synthase (Y. Evron, M. Avron [1990] Biochim Biophys Acta 1019: 115-120). The involvement of the ATP synthase [gamma]-subunit in the regulation of proton conductance was analyzed by measuring the effect of thiolalk...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.4.1549

    authors: Evron Y,Pick U

    更新日期:1997-12-01 00:00:00

  • A transglutaminase immunologically related to tissue transglutaminase catalyzes cross-linking of cell wall proteins in Chlamydomonas reinhardtii.

    abstract::The addition of primary amines to the growth medium of the unicellular green alga Chlamydomonas reinhardtii disrupts cell wall assembly in both vegetative and zygotic cells. Primary amines are competitive inhibitors of the protein-cross-linking activity of transglutaminases. Two independent assays for transglutaminase...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.121.3.1003

    authors: Waffenschmidt S,Kusch T,Woessner JP

    更新日期:1999-11-01 00:00:00

  • Leakage of Dhurrin and p-Hydroxybenzaldehyde from Young Sorghum Shoots Immersed in Various Solvents.

    abstract::Spectral scanning was used to provide estimates of the leakage of the cyanogenic glucoside, dhurrin (p-hydroxy-[S]-mandelonitrile-beta-d-glucoside), and its metabolite, p-hydroxybenzaldehyde (p-HB), from young light-grown shoots of Atlas sorghum (Sorghum bicolor [L.] Moench) when these shoots were immersed in water, t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.3.869

    authors: Haskins FA,Gorz HJ

    更新日期:1984-07-01 00:00:00

  • Mycorrhizal networks: common goods of plants shared under unequal terms of trade.

    abstract::Plants commonly live in a symbiotic association with arbuscular mycorrhizal fungi (AMF). They invest photosynthetic products to feed their fungal partners, which, in return, provide mineral nutrients foraged in the soil by their intricate hyphal networks. Intriguingly, AMF can link neighboring plants, forming common m...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.195727

    authors: Walder F,Niemann H,Natarajan M,Lehmann MF,Boller T,Wiemken A

    更新日期:2012-06-01 00:00:00

  • Metabolism of 5-methylthioribose to methionine.

    abstract::During ethylene biosynthesis, the H(3)CS- group of S-adenosylmethionine is released as 5'-methylthioadenosine, which is recycled to methionine via 5-methylthioribose (MTR). In mungbean hypocotyls and cell-free extracts of avocado, [(14)C]MTR was converted into labeled methionine via 2-keto-4-methylthiobutyric acid (KM...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.2.277

    authors: Miyazaki JH,Yang SF

    更新日期:1987-06-01 00:00:00

  • Transport of sodium into the xylem exudate of tobacco.

    abstract::When tobacco (Nicotiana tabacum L. var. Virginia Gold) plants were pretreated with Na ((22)Na) several days before detopping, from 2.3 to 4.9% of Na previously accumulated in roots appeared in the xylem exudate in 7 days after detopping. Na from the external medium, however, was readily transported to the exudate. Mor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.3.388

    authors: Wallace A,Abou-Zamzam AM,Mueller RT

    更新日期:1972-09-01 00:00:00

  • A New UV-A/B Protecting Pigment in the Terrestrial Cyanobacterium Nostoc commune.

    abstract::A new ultraviolet (UV)-A/B absorbing pigment with maxima at 312 and 330 nanometers from the cosmopolitan terrestrial cyanobacterium Nostoc commune is described. The pigment is found in high amounts (up to 10% of dry weight) in colonies grown under solar UV radiation but only in low concentrations in laboratory culture...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.4.1055

    authors: Scherer S,Chen TW,Böger P

    更新日期:1988-12-01 00:00:00

  • Modulation of Host Gene Expression during Initiation and Early Growth of Nodules in Cowpea, Vigna unguiculata (L.) Walp.

    abstract::Inoculation of 2-day-old cowpea (Vigna unguiculata [L.] Walp.) seedlings with Rhizobium fredii USDA257 results in proficient nodulation of the tap root. The most abundant nodulation occurs in a region roughly corresponding to the position of the root tip at the time of inoculation. We have examined plant gene expressi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.92.4.946

    authors: Trese AT,Pueppke SG

    更新日期:1990-04-01 00:00:00

  • Auxin asymmetry during gravitropism by tomato hypocotyls.

    abstract::Gravitropic asymmetry of auxin was observed in hypocotyls of tomato (Lycopersicon esculentum Mill.) soon after horizontal placement: the ratio of apically supplied [3H]IAA collected from the lower sides to that from the upper sides was about 1.4 between 5 and 10 minutes. This was adequately early to account for the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.2.652

    authors: Harrison MA,Pickard BG

    更新日期:1989-01-01 00:00:00

  • The glossy1 locus of maize and an epidermis-specific cDNA from Kleinia odora define a class of receptor-like proteins required for the normal accumulation of cuticular waxes.

    abstract::Mutations at the glossy1 (gl1) locus of maize (Zea mays L.) quantitatively and qualitatively affect the deposition of cuticular waxes on the surface of seedling leaves. The gl1 locus has been molecularly cloned by transposon tagging with the Mutator transposon system. The epi23 cDNA was isolated by subtractive hybridi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.4.1091

    authors: Hansen JD,Pyee J,Xia Y,Wen TJ,Robertson DS,Kolattukudy PE,Nikolau BJ,Schnable PS

    更新日期:1997-04-01 00:00:00

  • TAP46 plays a positive role in the ABSCISIC ACID INSENSITIVE5-regulated gene expression in Arabidopsis.

    abstract::TAP46 is a protein phosphatase2A (PP2A)-associated protein that regulates PP2A activity in Arabidopsis (Arabidopsis thaliana). To study how PP2A is involved in abscisic acid (ABA) signaling in plants, we studied the function of TAP46 in ABA-regulated seed maturation and seedling development. Expression of TAP46 coinci...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.233684

    authors: Hu R,Zhu Y,Shen G,Zhang H

    更新日期:2014-02-01 00:00:00

  • An intermediate in the synthesis of glucobrassicins from 3-indoleacetaldoxime by woad leaves.

    abstract::Leaves of woad (Isatis tinctoria L.) were found to incorporate efficiently tritiated indoleacetaldoxime and (35)S from (35)S-l-cystine into glucobrassicin and sulfoglucobrassicin. Time course of incorporation of (35)S from (35)S-cystine into the glucosinolates indicated that glucobrassicin was formed first and then su...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.1.43

    authors: Mahadevan S,Stowe BB

    更新日期:1972-07-01 00:00:00

  • Comparing the in vivo function of α-carboxysomes and β-carboxysomes in two model cyanobacteria.

    abstract::The carbon dioxide (CO2)-concentrating mechanism of cyanobacteria is characterized by the occurrence of Rubisco-containing microcompartments called carboxysomes within cells. The encapsulation of Rubisco allows for high-CO2 concentrations at the site of fixation, providing an advantage in low-CO2 environments. Cyanoba...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.237941

    authors: Whitehead L,Long BM,Price GD,Badger MR

    更新日期:2014-05-01 00:00:00

  • Inorganic carbon limitation induces transcripts encoding components of the CO(2)-concentrating mechanism in Synechococcus sp. PCC7942 through a redox-independent pathway.

    abstract::The cyanobacterial CO2-concentrating mechanism (CCM) allows photosynthesis to proceed in CO2-limited aquatic environments, and its activity is modulated in response to inorganic carbon (Ci) availability. Real-time reverse transcriptase-PCR analysis was used to examine the transcriptional regulation of more than 30 CCM...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.029728

    authors: Woodger FJ,Badger MR,Price GD

    更新日期:2003-12-01 00:00:00

  • Overexpression of acyl carrier protein-1 alters fatty acid composition of leaf tissue in Arabidopsis.

    abstract::Acyl carrier protein (ACP) is a small (9 kD) acidic protein that is an essential cofactor in plant fatty acid biosynthesis. Most plants have several isoforms of ACP, some of which are expressed constitutively and others that appear to be more tissue specific. Although the critical role of ACP in fatty acid biosynthesi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.127.1.222

    authors: Branen JK,Chiou TJ,Engeseth NJ

    更新日期:2001-09-01 00:00:00

  • Tobacco Plants Transformed with the Bean alphaai Gene Express an Inhibitor of Insect alpha-Amylase in Their Seeds.

    abstract::Bean (Phaseolus vulgaris L.) seeds contain a putative plant defense protein that inhibits insect and mammalian but not plant alpha-amylases. We recently (J Moreno, MJ Chrispeels [1989] Proc Natl Acad Sci USA 86:7885-7889) presented strong circumstantial evidence that this alpha-amylase inhibitor (alphaAI) is encoded b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.805

    authors: Altabella T,Chrispeels MJ

    更新日期:1990-06-01 00:00:00

  • Mutations in the TIR1 auxin receptor that increase affinity for auxin/indole-3-acetic acid proteins result in auxin hypersensitivity.

    abstract::The phytohormone auxin regulates virtually every aspect of plant development. The hormone directly mediates the interaction between the two members of the auxin coreceptor complex, a TRANSPORT INHIBITOR RESPONSE (TIR1)/AUXIN SIGNALING F-BOX protein and an AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) transcriptional repressor....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.215582

    authors: Yu H,Moss BL,Jang SS,Prigge M,Klavins E,Nemhauser JL,Estelle M

    更新日期:2013-05-01 00:00:00

  • Endoplasmic reticulum as a site of phenylpropanoid and flavonoid metabolism in hippeastrum.

    abstract::The nature of bound forms of enzymes of phenylpropanoid and flavonoid metabolism have been investigated in Hippeastrum CV Dutch Red Hybrid. Particulate components of petal homogenates were fractionated on sucrose gradients and the EDTA shift method was employed to characterize membranes of the endoplasmic reticulum. I...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.4.901

    authors: Wagner GJ,Hrazdina G

    更新日期:1984-04-01 00:00:00