Abstract:
:During ethylene biosynthesis, the H(3)CS- group of S-adenosylmethionine is released as 5'-methylthioadenosine, which is recycled to methionine via 5-methylthioribose (MTR). In mungbean hypocotyls and cell-free extracts of avocado, [(14)C]MTR was converted into labeled methionine via 2-keto-4-methylthiobutyric acid (KMB) and 2-hydroxy-4-methylthiobutyric acid (HMB), as intermediates. Incubation of [ribose-U-(14)C]MTR with avocado extract resulted in the production of [(14)C]formate, indicating the conversion of MTR to KMB involves a loss of formate, presumably from C-1 of MTR. Tracer studies showed that KMB was converted readily in vivo and in vitro to methionine, while HMB was converted much more slowly. The conversion of KMB to methionine by dialyzed avocado extract requires an amino donor. Among several potential donors examined, l-glutamine was the most efficient. Anaerobiosis inhibited only partially the oxidation of MTR to formate, KMB/HMB, and methionine by avocado extract. The role of O(2) in the conversion of MTR to methionine is discussed.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Miyazaki JH,Yang SFdoi
10.1104/pp.84.2.277subject
Has Abstractpub_date
1987-06-01 00:00:00pages
277-81issue
2eissn
0032-0889issn
1532-2548journal_volume
84pub_type
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