A dominant mutation in the light-oxygen and voltage2 domain vicinity impairs phototropin1 signaling in tomato.

Abstract:

:In higher plants, blue light (BL) phototropism is primarily controlled by the phototropins, which are also involved in stomatal movement and chloroplast relocation. These photoresponses are mediated by two phototropins, phot1 and phot2. Phot1 mediates responses with higher sensitivity than phot2, and phot2 specifically mediates chloroplast avoidance and dark positioning responses. Here, we report the isolation and characterization of a Nonphototropic seedling1 (Nps1) mutant of tomato (Solanum lycopersicum). The mutant is impaired in low-fluence BL responses, including chloroplast accumulation and stomatal opening. Genetic analyses show that the mutant locus is dominant negative in nature. In dark-grown seedlings of the Nps1 mutant, phot1 protein accumulates at a highly reduced level relative to the wild type and lacks BL-induced autophosphorylation. The mutant harbors a single glycine-1484-to-alanine transition in the Hinge1 region of a phot1 homolog, resulting in an arginine-to-histidine substitution (R495H) in a highly conserved A'α helix proximal to the light-oxygen and voltage2 domain of the translated gene product. Significantly, the R495H substitution occurring in the Hinge1 region of PHOT1 abolishes its regulatory activity in Nps1 seedlings, thereby highlighting the functional significance of the A'α helix region in phototropic signaling of tomato.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Sharma S,Kharshiing E,Srinivas A,Zikihara K,Tokutomi S,Nagatani A,Fukayama H,Bodanapu R,Behera RK,Sreelakshmi Y,Sharma R

doi

10.1104/pp.113.232306

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

2030-44

issue

4

eissn

0032-0889

issn

1532-2548

pii

pp.113.232306

journal_volume

164

pub_type

杂志文章
  • Water-stress-induced ethylene production in wheat : a fact or artifact?

    abstract::Effects of water stress on ethylene evolution from excised leaf segments and intact plants of wheat (Triticum aestivum L. cv Katepwa) were studied. Excised leaf segments of 8 day or 6 week old plants were dried until they lost 8% of their fresh weight (water potential about -2.3 megapascals). These and nondried contro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.2.406

    authors: Narayana I,Lalonde S,Saini HS

    更新日期:1991-06-01 00:00:00

  • Regulation of Corn Leaf Nitrate Reductase : II. Synthesis and Turnover of the Enzyme's Activity and Protein.

    abstract::The appearance and disappearance of NADH:nitrate reductase (NR) in the leaves of corn (Zea mays L. W64A x W182E) were studied using activity assays, an enzyme-linked immunosorbent assay (ELISA) and western blotting. N-starved, etiolated corn plants were treated with nutrients containing either 35 millimolar NH(4)-nitr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.2.442

    authors: Remmler JL,Campbell WH

    更新日期:1986-02-01 00:00:00

  • Solubilization of the starch-granule-bound starch synthase of normal maize kernels.

    abstract::The starch-granule-bound starch synthase from Zea mays has been solubilized with a recovery of between 50 and 84%. Chromatography of the solubilized enzyme on DEAE-Sepharose resolves two fractions of activity which may be distinguished by their response to citrate. Neither solubilized isoenzyme displays any significan...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.1.175

    authors: Macdonald FD,Preiss J

    更新日期:1983-09-01 00:00:00

  • Comparative freezing patterns in stems of cherry and azalea.

    abstract::Ice formation in stems, as determined by means of an electrophoretic mobility technique, occurs much more rapidly in azalea than in sour cherry. The difference is more marked in the bark than in the wood. Disrupting the structure of the tissues completely eliminates differences in freezing patterns, although gross ana...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.5.527

    authors: Dennis FG,Lumis GP,Olien CR

    更新日期:1972-11-01 00:00:00

  • Soybean Seed Water Relations during in Situ and in Vitro Growth and Maturation.

    abstract::Water, osmotic, and pressure potentials of soybean (Glycine max [L.] Merrill) embryos and related maternal tissues were measured during periods of seed growth and maturation to test the involvement of embryo water relations in seed maturation. Seeds were matured in situ or in an in vitro liquid culture medium in detac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.2.610

    authors: Saab IN,Obendorf RL

    更新日期:1989-02-01 00:00:00

  • Inhibition of ent-Kaurene Oxidation and Growth by alpha-Cyclopropyl-alpha-(p-methoxyphenyl)-5-pyrimidine Methyl Alcohol.

    abstract::Growth of Alaska peas (Pisum sativum) is inhibited more than 60% by alpha-cyclopropyl-alpha-(p-methoxyphenyl)-5-pyrimidine methyl alcohol (ancymidol) treatment. This growth inhibition can be reversed completely by gibberellic acid application. Cell-free enzyme preparations from pea shoot tips and wild cucumber (Marah ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.2.245

    authors: Coolbaugh RC,Hamilton R

    更新日期:1976-02-01 00:00:00

  • The effect of exogenous abscisic acid on stomatal development, stomatal mechanics, and leaf gas exchange in Tradescantia virginiana.

    abstract::Gas exchange parameters and stomatal physical properties were measured in Tradescantia virginiana plants grown under well-watered conditions and treated daily with either distilled water (control) or 3.0 mM abscisic acid (ABA). Photosynthetic capacity (CO(2) assimilation rate for any given leaf intercellular CO(2) con...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.2.935

    authors: Franks PJ,Farquhar GD

    更新日期:2001-02-01 00:00:00

  • Characterization of the procera tomato mutant shows novel functions of the SlDELLA protein in the control of flower morphology, cell division and expansion, and the auxin-signaling pathway during fruit-set and development.

    abstract::procera (pro) is a tall tomato (Solanum lycopersicum) mutant carrying a point mutation in the GRAS region of the gene encoding SlDELLA, a repressor in the gibberellin (GA) signaling pathway. Consistent with the SlDELLA loss of function, pro plants display a GA-constitutive response phenotype, mimicking wild-type plant...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.204552

    authors: Carrera E,Ruiz-Rivero O,Peres LE,Atares A,Garcia-Martinez JL

    更新日期:2012-11-01 00:00:00

  • Urea is a product of ureidoglycolate degradation in chickpea. Purification and characterization of the ureidoglycolate urea-lyase.

    abstract::A ureidoglycolate-degrading activity was analyzed in different organs of chickpea (Cicer arietinum). Activity was detected in all the tissues analyzed, but highest levels of specific activity were found in pods, from which it has been purified and characterized. This is the first ureidoglycolate-degrading activity tha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.2.828

    authors: Muñoz A,Piedras P,Aguilar M,Pineda M

    更新日期:2001-02-01 00:00:00

  • Adaptation of the Cyanobacterium Anabaena variabilis to Low CO(2) Concentration in Their Environment.

    abstract::The rate of adaptation of high CO(2) (5% v/v CO(2) in air)-grown Anabaena to a low level of CO(2) (0.05% v/v in air) was determined as a function of O(2) concentration. Exposure of cells to low (2.6%) O(2) concentration resulted in an extended lag in the adaptation to low CO(2) concentration. The rate of adaptation fo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.1.208

    authors: Marcus Y,Harel E,Kaplan A

    更新日期:1983-01-01 00:00:00

  • Compensation point and isotopic characteristics of c(3)/c(4) intermediates and hybrids in panicum.

    abstract::Leaf CO(2) compensation points and stable hydrogen, oxygen and carbon isotope ratios were determined for Panicum species including C(3)/C(4) intermediate photosynthesis plants, hybrids between C(3)/C(4) intermediates and C(3) plants, C(3) and C(4) plants in the Panicum genus as well as several other C(3) and C(4) plan...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.1.242

    authors: Sternberg Lda S,Deniro MJ,Sloan ME,Black CC

    更新日期:1986-01-01 00:00:00

  • Ketose reductase activity in developing maize endosperm.

    abstract::Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.3.830

    authors: Doehlert DC

    更新日期:1987-07-01 00:00:00

  • Dissection of the light signal transduction pathways regulating the two early light-induced protein genes in Arabidopsis.

    abstract::The expression of light-regulated genes in plants is controlled by different classes of photoreceptors that act through a variety of signaling molecules. During photomorphogenesis, the early light-induced protein (Elip) genes are among the first to be induced. To understand the light signal transduction pathways that ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Harari-Steinberg O,Ohad I,Chamovitz DA

    更新日期:2001-11-01 00:00:00

  • Abscisic Acid and cutout in cotton.

    abstract::A decline in growth, flowering, and boll (fruit) retention is referred to as cutout in cotton (Gossypium hirsutum L.). Fruit load affects cutout, possibly through hormonal effects. Experiments were conducted to test the hypothesis that fruits are a source of abscisic acid (ABA) that moves into fruiting branches and gr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.1.16

    authors: Guinn G

    更新日期:1985-01-01 00:00:00

  • Studies on the presence of adenosine cyclic 3':5'-monophosphate in oat coleoptiles.

    abstract::The incorporation of adenosine-8-(14)C into adenosine cyclic 3':5'-monophosphate in coleoptile-first leaf segments of Avena sativa L. was investigated. Homogenates of segments incubated in adenosine-8-(14)C for either 4 or 10 hours were partially purified by thin layer chromatography followed by paper electrophoresis....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.2.346

    authors: Ownby JD,Ross CW

    更新日期:1975-02-01 00:00:00

  • Protein cryoprotective activity of a cytosolic small heat shock protein that accumulates constitutively in chestnut stems and is up-regulated by low and high temperatures.

    abstract::Heat shock, and other stresses that cause protein misfolding and aggregation, trigger the accumulation of heat shock proteins (HSPs) in virtually all organisms. Among the HSPs of higher plants, those belonging to the small HSP (sHSP) family remain the least characterized in functional terms. We analyzed the occurrence...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.035857

    authors: Lopez-Matas MA,Nuñez P,Soto A,Allona I,Casado R,Collada C,Guevara MA,Aragoncillo C,Gomez L

    更新日期:2004-04-01 00:00:00

  • Plastidic Δ6 Fatty-Acid Desaturases with Distinctive Substrate Specificity Regulate the Pool of C18-PUFAs in the Ancestral Picoalga Ostreococcus tauri.

    abstract::Eukaryotic Δ6-desaturases are microsomal enzymes that balance the synthesis of ω-3 and ω-6 C18-polyunsaturated fatty acids (C18-PUFAs) according to their specificity. In several microalgae, including Ostreococcus tauri, plastidic C18-PUFAs are strictly regulated by environmental cues suggesting an autonomous control o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00281

    authors: Degraeve-Guilbault C,Gomez RE,Lemoigne C,Pankansem N,Morin S,Tuphile K,Joubès J,Jouhet J,Gronnier J,Suzuki I,Coulon D,Domergue F,Corellou F

    更新日期:2020-09-01 00:00:00

  • The mechanism of iron homeostasis in the unicellular cyanobacterium synechocystis sp. PCC 6803 and its relationship to oxidative stress.

    abstract::In this article, we demonstrate the connection between intracellular iron storage and oxidative stress response in cyanobacteria. Iron is essential for the survival of all organisms. However, the redox properties that make iron a valuable cofactor also lead to oxidative interactions, resulting in the formation of harm...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.141853

    authors: Shcolnick S,Summerfield TC,Reytman L,Sherman LA,Keren N

    更新日期:2009-08-01 00:00:00

  • Peroxygenase-Catalyzed Fatty Acid Epoxidation in Cereal Seeds (Sequential Oxidation of Linoleic Acid into 9(S),12(S),13(S)-Trihydroxy-10(E)-Octadecenoic Acid).

    abstract::Peroxygenase-catalyzed epoxidation of oleic acid in preparations of cereal seeds was investigated. The 105,000g particle fraction of oat (Avena sativa) seed homogenate showed high peroxygenase activity, i.e. 3034 [plus or minus] 288 and 2441 [plus or minus] 168 nmol (10 min)-1 mg-1 protein in two cultivars, whereas th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.3.807

    authors: Hamberg M,Hamberg G

    更新日期:1996-03-01 00:00:00

  • LEPS2, a phosphorus starvation-induced novel acid phosphatase from tomato.

    abstract::Phosphate (Pi) is one of the least available plant nutrients found in the soil. A significant amount of phosphate is bound in organic forms in the rhizosphere. Phosphatases produced by plants and microbes are presumed to convert organic phosphorus into available Pi, which is absorbed by plants. In this study we descri...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.2.728

    authors: Baldwin JC,Karthikeyan AS,Raghothama KG

    更新日期:2001-02-01 00:00:00

  • Arabidopsis HMA2, a divalent heavy metal-transporting P(IB)-type ATPase, is involved in cytoplasmic Zn2+ homeostasis.

    abstract::PIB-type ATPases transport heavy metal ions (Cu+, Cu2+, Zn2+, Cd2+, Co2+, etc.) across biological membranes. Several members of this subfamily are present in plants. Higher plants are the only eukaryotes where putative Zn(2+)-ATPases have been identified. We have cloned HMA2, a PIB-ATPase present in Arabidopsis (Arabi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.046292

    authors: Eren E,Argüello JM

    更新日期:2004-11-01 00:00:00

  • Reduced Lignin Content and Altered Lignin Composition in Transgenic Tobacco Down-Regulated in Expression of L-Phenylalanine Ammonia-Lyase or Cinnamate 4-Hydroxylase.

    abstract::We analyzed lignin content and composition in transgenic tobacco (Nicotiana tabacum) lines altered in the expression of the early phenylpropanoid biosynthetic enzymes L-phenylalanine ammonia-lyase and cinnamate 4-hydroxylase (C4H). The reduction of C4H activity by antisense expression or sense suppression resulted in ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.1.41

    authors: Sewalt V,Ni W,Blount JW,Jung HG,Masoud SA,Howles PA,Lamb C,Dixon RA

    更新日期:1997-09-01 00:00:00

  • Separation of the Mg-ATPases from the Ca-Phosphatase Activity of Microsomal Membranes Prepared from Barley Roots.

    abstract::Two methods for preparing membrane fractions from barley (Hordeum vulgare cv California Mariout 72) roots were compared in order to resolve reported differences between the characteristics of the plasma membrane ATPase of barley and that of other species. When microsomal membranes were prepared by a published procedur...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.4.857

    authors: Dupont FM,Hurkman WJ

    更新日期:1985-04-01 00:00:00

  • The mechanic state of "inner tissue" in the growing zone of sunflower hypocotyls and the regulation of its growth rate following excision.

    abstract::Spontaneous growth of isolated inner tissue from the etiolated sunflower (Helianthus annuus L.) hypocotyl growing zone was investigated. A new preparation technique allowed measurements starting 3 s after excision. Elongation with respect to the turgescent and plasmolized state was quantified in terms of relative grow...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.123.2.605

    authors: Peters WS,Tomos AD

    更新日期:2000-06-01 00:00:00

  • Characterization of Hypoxically Inducible Lactate Dehydrogenase in Maize.

    abstract::Oxygen deprivation induces a wide variety of genes, but the most extensively studied are those encoding enzymes of the glycolytic pathway. Lactate dehydrogenase (LDH, EC 1.1.1.27) activity increases up to 3.5-fold in maize (Zea mays L.) roots during several days of hypoxic induction. This increase in activity is accom...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.3.1015

    authors: Christopher ME,Good AG

    更新日期:1996-11-01 00:00:00

  • Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes.

    abstract::The Dof (DNA binding with one finger) transcriptional activator rice (Oryza sativa) prolamin box binding factor (RPBF), which is involved in gene regulation of rice seed storage proteins, has been isolated from rice cDNA expressed sequence tag clones containing the conserved Dof. RPBF is found as a single gene per hap...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.082826

    authors: Yamamoto MP,Onodera Y,Touno SM,Takaiwa F

    更新日期:2006-08-01 00:00:00

  • Arabidopsis RING E3 ligase XBAT32 regulates lateral root production through its role in ethylene biosynthesis.

    abstract::XBAT32, a member of the RING domain-containing ankyrin repeat subfamily of E3 ligases, was previously identified as a positive regulator of lateral root development. Arabidopsis (Arabidopsis thaliana) plants harboring a mutation in XBAT32 produce fewer lateral roots that wild-type plants. We found that xbat32 mutants ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.156976

    authors: Prasad ME,Schofield A,Lyzenga W,Liu H,Stone SL

    更新日期:2010-08-01 00:00:00

  • Ozone-induced Fatty Acid and Viability Changes in Chlorella.

    abstract::Ozone-treated cells of the photosynthetic green alga Chlorella sorokiniana var. pacificensis exhibit an exponential decline in viability, as measured by their ability to form colonies on agar plates. Postexposure conditions appear to have little, if any, effect on this rate of decline. Except in young (early exponenti...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.1.15

    authors: Frederick PE,Heath RL

    更新日期:1975-01-01 00:00:00

  • Localization, solubilization and characterization of plant membrane-associated calcium-dependent protein kinases.

    abstract::Membrane fractions from mature silver beet (Beta vulgaris) deveined leaf and leaf stem homogenates have associated Ca(2+) -dependent protein kinase. The Ca(2+) -dependent protein kinase activity is associated with plasma membranes (density 1.14-1.18 grams per cubic centimeter) as determined from copurification on isop...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.1.164

    authors: Klucis E,Polya GM

    更新日期:1988-09-01 00:00:00

  • Heterogeneous distribution of glycosyltransferases in the endoplasmic reticulum of castor bean endosperm.

    abstract::Endoplasmic reticulum membranes stripped of attached ribosomes were isolated from homogenates of germinating castor bean (Ricinus communis L.) endosperm by sucrose density gradient centrifugation. The isolated endoplasmic reticulum fraction was further separated into two major membrane subfractions by centrifugation o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.2.547

    authors: Conder MJ,Lord JM

    更新日期:1983-06-01 00:00:00