LEPS2, a phosphorus starvation-induced novel acid phosphatase from tomato.

abstract：:Two cDNAs encoding functional carbonic anhydrase (CA) enzymes were recently isolated from a non-photosynthetic, cotyledon library of cotton (Gossypium hirsutum) seedlings with putative plastid-targeting sequences (GenBank accession nos. AF132854 and AF132855). Relative CA transcript abundance and enzyme activity incre...

journal_title：Plant physiology

authors： Hoang CV,Chapman KD

更新日期：2002-04-01 00:00:00

abstract：:The effect of the proline analog azetidine-2-carboxylic acid (Aze) on the induction and the regulation of heat-shock (HS) mRNA accumulation and heat-shock protein (HSP) synthesis in soybean (Glycine max) seedlings was studied. Treatment with Aze elicited an HS-like response at the normal growth temperature, 28[deg]C, ...

journal_title：Plant physiology

authors： Lee Y,Nagao RT,Lin CY,Key JL

更新日期：1996-01-01 00:00:00

abstract：:Pectin methylesterase (PME), polygalacturonase (PG), xylanase, cellulase, and proteinase activity were determined and related to respiration, ethylene evolution, and changes in skin color of papaya (Carica papaya L.) fruit from harvest through to the start of fruit breakdown. PME gradually increased from the start of ...

journal_title：Plant physiology

authors： Paull RE,Chen NJ

更新日期：1983-06-01 00:00:00

abstract：:Two-dimensional electrophoresis was performed on proteins of bundle sheath and mesophyll cells isolated from the C(4) grass Digitaria sanguinalis (L.) Scop. Two-dimensional maps of these proteins were constructed and ribulose-1,5-biphosphate carboxylase and phosphoenolpyruvate carboxylase were identified. Of the total...

journal_title：Plant physiology

authors： Harrison PA,Black CC

更新日期：1982-11-01 00:00:00

abstract：:Barley (c.v. Himalaya) aleurone layers were incubated in [(3)H]gibberellin A(1) (GA(1)) at low temperatures. At 3 and 4 C, (3)H-activity was steadily accumulated in aleurone layers, and this accumulation was correlated with significant [(3)H]GA(1) metabolism. At 1 and 1.5 C, metabolism could not be detected, and at th...

journal_title：Plant physiology

authors： Keith B,Boal R,Srivastava LM

更新日期：1980-11-01 00:00:00

abstract：:Tonoplast vesicles were purified from a microsomal fraction isolated from etiolated soybean hypocotyls (Glycine max L.) by preparative free-flow electrophoresis. Marker enzyme determinations and immunoblot analysis against the vacuolar-ATPase confirmed the nature and the purity of the isolated membranes. A purified to...

journal_title：Plant physiology

authors： Canut H,Brightman A,Boudet AM,Morré DJ

更新日期：1990-11-01 00:00:00

abstract：:beta-Glucan synthetase activity in growing regions of pea (Pisum sativum L.) epicotyls was assayed by supplying UDP-glucose to particulate fractions of tissue homogenates or to thin tissue slices. Particulate fractions are less active in forming alkali-insoluble glucan than slices from the same tissue, although many k...

journal_title：Plant physiology

authors： Raymond Y,Fincher GB,Maclachlan GA

更新日期：1978-06-01 00:00:00

abstract：:Analysis of gene expression data generated by high-throughput microarray transcript profiling experiments has demonstrated that genes with an overall similar expression pattern are often enriched for similar functions. This guilt-by-association principle can be applied to define modular gene programs, identify cis-reg...

journal_title：Plant physiology

authors： Vandepoele K,Quimbaya M,Casneuf T,De Veylder L,Van de Peer Y

更新日期：2009-06-01 00:00:00

abstract：:The local anesthetic dibucaine inhibited respiration-dependent contraction mediated by the K(+)/H(+) antiport system of isolated corn mitochondria. Respiration declined concurrently. Nigericin, an exogenous K(+)/H(+) exchanger, restored ion efflux in dibucaine-blocked corn mitochondria. It was concluded that dibucaine...

journal_title：Plant physiology

authors： Klein RR,Koeppe DE

更新日期：1985-04-01 00:00:00

abstract：:The first committed step in the conversion of cycloartenol into Delta(5) C24-alkyl sterols in plants is catalyzed by an S-adenosyl-methionine-dependent sterol-C24-methyltransferase type 1 (SMT1). We report the consequences of overexpressing SMT1 in tobacco (Nicotiana tabacum), under control of either the constitutive ...

journal_title：Plant physiology

authors： Holmberg N,Harker M,Gibbard CL,Wallace AD,Clayton JC,Rawlins S,Hellyer A,Safford R

更新日期：2002-09-01 00:00:00

abstract：:The effect of seed coat removal on the synthesis of alpha-amylase isoenzymes in wheat was investigated. The immature wheat endosperm-aleurone (seed coat and embryo detached) produced considerably less alpha-amylase activity than immature whole or de-embryonated wheat kernels, when incubated under identical conditions ...

journal_title：Plant physiology

authors： Marchylo BA,Lacroix LJ,Kruger JE

更新日期：1981-01-01 00:00:00

abstract：:Through a single genetic transformation in onion (Allium cepa), a crop recalcitrant to genetic transformation, we suppressed the lachrymatory factor synthase gene using RNA interference silencing in six plants. This reduced lachrymatory synthase activity by up to 1,544-fold, so that when wounded the onions produced si...

journal_title：Plant physiology

authors： Eady CC,Kamoi T,Kato M,Porter NG,Davis S,Shaw M,Kamoi A,Imai S

更新日期：2008-08-01 00:00:00

abstract：:The phosphohydrolase activity of a light microsomal fraction isolated from corn roots (Zea mays L. cv LG 55) was investigated. The fraction, which appears to be enriched in endoplasmic reticulum and Golgi membranes, has ATPase and pyrophosphatase activities that hydrolyze ATP and pyrophosphate at an optimum pH of 7.0,...

journal_title：Plant physiology

authors： Vicente JA,Vale MG

更新日期：1991-08-01 00:00:00

abstract：:TILLING (for Targeting Induced Local Lesions IN Genomes) is a well-established method for identifying plants carrying point mutations in genes of interest. A traditional TILLING project requires a significant investment of time and resources to establish the mutant population and screening infrastructure. Here, we des...

journal_title：Plant physiology

authors： Bush SM,Krysan PJ

更新日期：2010-09-01 00:00:00

abstract：:Plumeria (Plumeria rubra), well known for its brightly colored and fragrant flowers, emits a number of floral volatile organic compounds (VOCs). Plumeria flowers emit a total of 43 VOCs including nine phenylpropanoids/benzenoids, such as 2-phenylethanol (2PE), benzaldehyde, 2-phenylacetaldehyde (PAld), (E/Z)-phenylace...

journal_title：Plant physiology

authors： Dhandapani S,Jin J,Sridhar V,Chua NH,Jang IC

更新日期：2019-05-01 00:00:00

abstract：:In vivo pyruvate synthesis by malic enzyme (ME) and pyruvate kinase and in vivo malate synthesis by phosphoenolpyruvate carboxylase and the Krebs cycle were measured by 13C incorporation from [1-13C]glucose into glucose-6-phosphate, alanine, glutamate, aspartate, and malate. These metabolites were isolated from maize ...

journal_title：Plant physiology

authors： Edwards S,Nguyen BT,Do B,Roberts JKM

更新日期：1998-03-01 00:00:00

abstract：:Injection of [(14)C]tryptophan into one seed in a soybean fruit resulted in recovery of radiolabel in a fraction that cochromatographed with indoleacetic acid (IAA) through three successive high performance liquid chromatography separations. Label was found in the putative IAA fraction in all of the fruit tissues samp...

journal_title：Plant physiology

authors： Hein MB,Brenner ML,Brun WA

更新日期：1986-10-01 00:00:00

abstract：:Following excision, etiolated epicotyl segments of Pisum sativum L. cv. Alaska exhibit a marked hyperpolarization of membrane potential which is followed by a linear accumulation of K(+) when segments are incubated in Higinbotham nutrient solution. Segments aged for several hours and then reexcised display only a slig...

journal_title：Plant physiology

authors： Pierce WS

更新日期：1981-04-01 00:00:00

abstract：:Microsomal membranes from suspension-cultured Catharanthus roseus cells possess an enzymic activity that catalyzes the ATP-dependent phosphorylation of phosphatidic acid (PA) to form diacylglycerol pyrophosphate (H. Behrbohm, J.B. Wissing [1993] FEBS Lett 315: 95-99). This enzyme activity, PA kinase, was purified and ...

journal_title：Plant physiology

authors： Wissing JB,Behrbohm H

更新日期：1993-08-01 00:00:00

abstract：:Ascorbic acid is synthesized from galactono-gamma-lactone (GL) in plant tissues. An improved extraction procedure involving ammonium sulfate precipitation of membrane proteins from crude leaf homogenates yielded a simple, quick method for determining tissue activities of galactono-gamma-lactone dehydrogenase (GLDH). T...

journal_title：Plant physiology

authors： Bartoli CG,Pastori GM,Foyer CH

更新日期：2000-05-01 00:00:00

abstract：:Fucoid algae release gametes into seawater following an inductive light period (potentiation), and gamete expulsion from potentiated receptacles of Pelvetia compressa began about 2 min after a light-to-dark transition. Agitation of the medium reversed potentiation, with an exponential time course completed in about 3 ...

journal_title：Plant physiology

authors： Pearson GA,Howard Brawley S

更新日期：1998-09-01 00:00:00

abstract：:Superoxide dismutases (SODs) are ubiquitous metalloenzymes that catalyze the dismutation of superoxide radicals. Chloroplasts have two isozymes, copper/zinc SOD (Cu/ZnSOD) and iron SOD (FeSOD), encoded by nuclear genes. Because bryophytes are considered as the earliest land plants, they are one of the most interesting...

journal_title：Plant physiology

authors： Nagae M,Nakata M,Takahashi Y

更新日期：2008-04-01 00:00:00

abstract：:Large changes in indole-3-acetic acid (IAA) levels occur during growth of Lemna gibba G-3 in sterile culture. The levels of IAA were measured in plants during a 45 day growth cycle using HPLC and isotope dilution analysis followed by selected ion current monitoring GC-MS analysis with (13)C(6)-IAA as the internal stan...

journal_title：Plant physiology

authors： Slovin JP,Cohen JD

更新日期：1988-02-01 00:00:00

abstract：:The JASMONATE ZIM DOMAIN (JAZ) proteins function as negative regulators of jasmonic acid signaling in plants. We cloned 12 JAZ genes from native tobacco (Nicotiana attenuata), including nine novel JAZs in tobacco, and examined their expression in plants that had leaves elicited by wounding or simulated herbivory. Most...

journal_title：Plant physiology

authors： Oh Y,Baldwin IT,Gális I

更新日期：2012-06-01 00:00:00

abstract：:We have characterized two related cDNAs (RCI2A and RCI2B) corresponding to genes from Arabidopsis thaliana, the expression of which is transiently induced by low, nonfreezing temperatures. RCI2A and RCI2B encode small (54 amino acids), highly hydrophobic proteins that bear two potential transmembrane domains. They sho...

journal_title：Plant physiology

authors： Capel J,Jarillo JA,Salinas J,Martínez-Zapater JM

更新日期：1997-10-01 00:00:00

abstract：:Filaments of the blue-green alga Anabaena variabilis permeabilized by dimethylsulfoxide (DMSO) produce increased amounts of 5-aminolevulinate in the presence of levulinic acid. The metabolic activity of the filaments remains unperturbed in the presence of up to 7.5% (v/v) DMSO. Studies utilizing DMSO-permeabilized fil...

journal_title：Plant physiology

authors： Avissar YJ

更新日期：1983-05-01 00:00:00

abstract：:We examined the response of leaves of 3-week-old maize (Zea mays L.) to short-term (5 h) fumigation with O3-enriched air (0, 0.12, 0.24, or 0.36 [mu]L/L). Older leaves and leaf tissue developed more severe visible damage at higher external O3 concentrations. To investigate the immediate effect of O3 exposure on the ac...

journal_title：Plant physiology

authors： Pino ME,Mudd JB,Bailey-Serres J

更新日期：1995-06-01 00:00:00

abstract：:A water-soluble chlorophyll protein (WSCP) in cauliflower (Brassica oleracea L.) was purified and its N-terminal sequence was determined. Forty-six of 48 residues of the sequence completely matched those of the drought-induced 22-kD protein (BnD22) in rapeseed (Brassica napus L.). All 40 sequenced residues of WSCP fro...

journal_title：Plant physiology

authors： Nishio N,Satoh H

更新日期：1997-10-01 00:00:00

abstract：:The response of cortical microtubules to low temperature and freezing was assessed for root tips of cold-acclimated and non-acclimated winter rye (Secale cereale L. cv Puma) seedlings using indirect immunofluorescence microscopy with antitubulin antibodies. Roots cooled to 0 or -3 degrees C were fixed for immunofluore...

journal_title：Plant physiology

authors： Kerr GP,Carter JV

更新日期：1990-05-01 00:00:00

abstract：:Starch biosynthetic enzymes from maize (Zea mays) and wheat (Triticum aestivum) amyloplasts exist in cell extracts in high molecular weight complexes; however, the nature of those assemblies remains to be defined. This study tested the interdependence of the maize enzymes starch synthase IIa (SSIIa), SSIII, starch bra...

journal_title：Plant physiology

authors： Hennen-Bierwagen TA,Lin Q,Grimaud F,Planchot V,Keeling PL,James MG,Myers AM

更新日期：2009-03-01 00:00:00