Two-Dimensional Electrophoretic Mapping of Proteins of Bundle Sheath and Mesophyll Cells of the C(4) Grass Digitaria sanguinalis (L.) Scop. (Crabgrass).

Abstract:

:Two-dimensional electrophoresis was performed on proteins of bundle sheath and mesophyll cells isolated from the C(4) grass Digitaria sanguinalis (L.) Scop. Two-dimensional maps of these proteins were constructed and ribulose-1,5-biphosphate carboxylase and phosphoenolpyruvate carboxylase were identified. Of the total number of proteins found in both cell types, 36% were found only in bundle sheath cells, 17% only in mesophyll cells, and 47% in both cell types. By comparison, the distributions of 48 enzymes assayed in these cell types were 35%, 21%, and 44%, respectively.Protein patterns were also compared with C(4) plants exhibiting different decarboxylation pathways and, in both bundle sheath and mesophyll cells, proteins were found which were unique to each species. Bundle sheath proteins of one C(4) species were found to be more like bundle sheath proteins of another C(4) species than like mesophyll proteins of the same species.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Harrison PA,Black CC

doi

10.1104/pp.70.5.1359

subject

Has Abstract

pub_date

1982-11-01 00:00:00

pages

1359-66

issue

5

eissn

0032-0889

issn

1532-2548

journal_volume

70

pub_type

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