Abstract:
:The appearance and disappearance of NADH:nitrate reductase (NR) in the leaves of corn (Zea mays L. W64A x W182E) were studied using activity assays, an enzyme-linked immunosorbent assay (ELISA) and western blotting. N-starved, etiolated corn plants were treated with nutrients containing either 35 millimolar NH(4)-nitrate or K-nitrate and immediately thereafter given light. The curve for enhancement of NR activity had three phases: 1 hour lag, 5 hour rapid increase, and steady state. The pattern for NR protein, as measured with the ELISA, also had three phases, but the increase was more rapid and the steady state was established earlier. To differentiate the effects of N nutrition from those of light, N-starved etiolated plants were given N nutrients 4 hours before light. During the dark pretreatment, NR activity and protein increased. When the light was turned on the NR activity and protein increased very rapidly without a lag. Western blots of polyacrylamide gels of native and denatured crude extracts showed that NADH:NR polypeptide was absent prior to treatment with N nutrients, but appeared after nitrate was given in dark or light. A low level of NR activity was found in N-starved, etiolated plants and it was shown by western blotting to be an NR form with a different electrophoretic mobility in nondenaturing gels. Since this minor NR form was not influenced by either nitrate or light, it was designated a constitutive NR. Dark decay of NR activity and protein was also studied. After the plants which had been in light with N nutrients for 24 hours were transferred to dark, the NR activity dropped by 30% within 1 hour, but the NR protein did not decrease. This inactivation of NR was further supported by returning the plants to the light after 1.5 hours of dark and finding the activity restored without change in NR protein. After the initial activity drop, a parallel decrease in NR activity and protein was observed, which was likely due to irreversible degradation by proteolysis.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Remmler JL,Campbell WHdoi
10.1104/pp.80.2.442subject
Has Abstractpub_date
1986-02-01 00:00:00pages
442-7issue
2eissn
0032-0889issn
1532-2548journal_volume
80pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Circular RNAs (circRNAs) play roles in various biological processes, but their functions in the rice (Oryza sativa) response to Magnaporthe oryzae remain elusive. Here, we demonstrate that circRNAs are involved in the rice-M. oryzae interaction using comparative circRNA-sequencing and transgenic approaches. We identif...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.00716
更新日期:2020-01-01 00:00:00
abstract::trans-Acting small interfering RNAs (tasiRNAs) participate in the regulation of organ morphogenesis and determination of developmental timing in plants by down-regulating target genes through mRNA cleavage. The production of tasiRNAs is triggered by microRNA173 (miR173) and other specific microRNA-mediated cleavage of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00148
更新日期:2016-05-01 00:00:00
abstract::Changes in acyl lipids and pigments during leaf development in a virescens barley mutant (M) and the normal (N) were studied. Apical 3-cm leaf segments were extracted with chloroform-methanol, the extracts were purified on Sephadex G-25 columns, and the polar lipids were separated on two-dimensional-thin layer chromat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.4.646
更新日期:1981-04-01 00:00:00
abstract::The Mg-nucleoside triphosphatase activity associated with the inner envelope membrane of the pea chloroplast is comprised of at least two components, a major activity that is sensitive to vanadate and sodium fluoride and a minor insensitive activity. The vanadate/fluoride sensitive activity has been partially purified...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.4.908
更新日期:1986-04-01 00:00:00
abstract::Virus-induced gene silencing (VIGS) is used extensively for gene function studies in plants. VIGS is inexpensive and rapid compared with silencing conducted through stable transformation, but many virus-silencing vectors, especially in grasses, induce only transient silencing phenotypes. A major reason for transient p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00905
更新日期:2018-01-01 00:00:00
abstract::To study in detail the hypersensitive reaction, one of the major defense responses of plants against microbial infection, we used a model system of reduced complexity with cultured parsley (Petroselinum crispum) cells infected with the phytopathogenic fungus Phytophthora infestans. Experimental conditions were establi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.1.433
更新日期:1996-09-01 00:00:00
abstract::Short exposure of the spores of Cheilanthes farinosa to low intensity red light promotes their germination, which is not reversed by a subsequent exposure to far red light. Germination is, however, inhibited by blue light administered before or after red light. Inhibition of germination by blue light is annulled by ex...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.2.306
更新日期:1973-02-01 00:00:00
abstract::Field-grown tuber-bearing potatoes were screened for frost tolerance in a late stage of development. Three different clones of Solanum tuberosum L. and two interspecific crosses between clones of S. tuberosum and the wild potato species S. demissum Lindl. were studied. Two different methods were used. (a) Temperature-...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.5.1299
更新日期:1982-11-01 00:00:00
abstract::The DEFECTIVE KERNEL1 (DEK1) calpain is a conserved 240-kD key regulator of three-dimensional body patterning in land plants acting via mitotic cell plane positioning. The activity of the cytosolic C-terminal calpain protease is regulated by the membrane-anchored DEK1 MEM, which is connected to the calpain via the 600...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00925
更新日期:2016-10-01 00:00:00
abstract::The effects of nitrogen source NO(3) (-) or NH(4) (+) on nitrogen metabolism during the first 2 weeks of germination of the rice seedling (Oryza sativa L., var. IR22) grown in nutrient solution containing 40 mug/ml N were studied. Total, soluble protein, and free amino N levels were higher in the NH(4) (+)-grown seedl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.57.6.923
更新日期:1976-06-01 00:00:00
abstract::The seeds of lettuce (Lactuca sativa L. cv. Grand Rapids) germinate in darkness at 25 C when treated by gibberellic acid (GA(3)) for 1 hour following 2 hours of imbibition. The time of GA(3) application influences the rate and the final percentage of seeds that germinate. In contrast, red light illumination given at d...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.4.575
更新日期:1977-10-01 00:00:00
abstract::Since peroxidase and manganese have been implicated in both auxin destruction and ethylene production, the effect of auxins and high tissue levels of manganese on the peroxidative indoleacetic acid oxidase system and the internal level of ethylene was determined in cotton (Gossypium hirsutum L. cv. Watson GL-7). The h...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.4.555
更新日期:1972-04-01 00:00:00
abstract::For an efficient defense response against pathogens, plants must coordinate rapid genetic reprogramming to produce an incompatible interaction. Nitrate Trasnporter2 (NRT2) gene family members are sentinels of nitrate availability. In this study, we present an additional role for NRT2.1 linked to plant resistance again...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.184424
更新日期:2012-02-01 00:00:00
abstract::A Sclerotinia enzyme preparation with predominant exo-beta-(1-->3)-glucanase activity has the capacity to mediate the formation of tetrasaccharide from 3-O-beta-cellobiosyl-d-glucose or cellotriose, and a pentasaccharide from 3-O-beta-cellotriosyl-d-glucose or cellotetraose. Transglucosylation is not observed when the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.2.193
更新日期:1979-08-01 00:00:00
abstract::A novel mutant of Arabidopsis (Arabidopsis thaliana), having highly glossy inflorescence stems, postgenital fusion in floral organs, and reduced fertility, was isolated from an ethyl methanesulfonate-mutagenized population and designated glossyhead1 (gsd1). The gsd1 locus was mapped to chromosome 1, and the causal gen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.185132
更新日期:2011-11-01 00:00:00
abstract::Using photoacoustic spectroscopy, state 1-state 2 transitions were demonstrated in vivo in intact sugar maple leaves (Acer saccharum Marsh.) by following the changes in energy storage of photosystems (PS) I and II. Energy storage measured with 650 nm modulated light (light 2) in the presence of background white light ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.1.330
更新日期:1991-09-01 00:00:00
abstract::The LOSS OF APOMEIOSIS (LOA) locus is one of two dominant loci known to control apomixis in the eudicot Hieracium praealtum. LOA stimulates the differentiation of somatic aposporous initial cells after the initiation of meiosis in ovules. Aposporous initial cells undergo nuclear proliferation close to sexual megaspore...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.181164
更新日期:2011-11-01 00:00:00
abstract::Explants of genetic tumors, tumors initiated by Agrobacterium tumefaciens strains B-6 and T-37, and excised pith plugs from Nicotiana glauca, N. langsdorffii, and N. glauca-langsdorffii were cultured on Murashige and Skoog medium. All cultures, pith callus and tumors with the exception of N. langsdorffii pith grew on ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.7.1073
更新日期:1969-07-01 00:00:00
abstract::To dissect the expression of the psbB gene cluster of the Chlamydomonas reinhardtii chloroplast genome and to assess the role of the photosystem II H-phosphoprotein (PSII-H) in the biogenesis and/or stabilization of PSII, an aadA gene cassette conferring spectinomycin resistance was employed for mutagenesis. Disruptio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.4.1359
更新日期:1997-04-01 00:00:00
abstract::Glutamate (Glu) metabolism and amino acid translocation were investigated in the young and old leaves of tobacco (Nicotiana tabacum L. cv Xanthi) using [15N]ammonium and [2-15N]Glu tracers. Regardless of leaf age, [15N]ammonium assimilation occurred via glutamine synthetase (GS; EC 6.1.1.3) and Glu synthase (ferredoxi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.071910
更新日期:2006-02-01 00:00:00
abstract::Light is a major environmental factor required for stomatal opening. Blue light (BL) induces stomatal opening in higher plants as a signal under the photosynthetic active radiation. The stomatal BL response is not present in the fern species of Polypodiopsida. The acquisition of a stomatal BL response might provide co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00134
更新日期:2015-10-01 00:00:00
abstract::The accumulation and reduction of nitrate in the presence of the nitrogen metabolites asparagine (Asn) and glutamine (Gln) and the carbon metabolite sucrose (Suc) were examined in maize (Zea mays L.) seedlings in an attempt to separate their effects on the nitrate uptake system and the nitrate reduction system. After ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.2.583
更新日期:1997-06-01 00:00:00
abstract::Gibberellic acid (GA) enhances the synthesis of alpha-amylase and ribonuclease in isolated aleurone layers and this process is inhibited by abscisin. Removal of gibberellic acid in mid-course of alpha-amylase production results in a slowing down of alpha-amylase synthesis, suggesting a continued requirement of GA for ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.7.1008
更新日期:1967-07-01 00:00:00
abstract::The Dof (DNA binding with one finger) transcriptional activator rice (Oryza sativa) prolamin box binding factor (RPBF), which is involved in gene regulation of rice seed storage proteins, has been isolated from rice cDNA expressed sequence tag clones containing the conserved Dof. RPBF is found as a single gene per hap...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.082826
更新日期:2006-08-01 00:00:00
abstract::The trans-Golgi network (TGN) plays a central role in cellular secretion and has been implicated in sorting cargo destined for the plasma membrane. Previously, the Arabidopsis (Arabidopsis thaliana) echidna (ech) mutant was shown to exhibit a dwarf phenotype due to impaired cell expansion. However, ech also has a prev...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.227769
更新日期:2014-03-01 00:00:00
abstract::The concentration-dependent stimulatory and inhibitory effect of N-glycans on tomato (Lycopersicon esculentum Mill.) fruit ripening was recently reported (B. Priem and K.C. Gross [1992] Plant Physiol 98: 399-401). We report here the structure of 10 free N-glycans in mature green tomatoes. N-Glycans were purified from ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.2.445
更新日期:1993-06-01 00:00:00
abstract::Flowering of the facultative long-day plant Arabidopsis is controlled by several endogenous and environmental factors, among them gibberellins (GAs) and day length. The promotion of flowering by long days involves an endogenous clock that interacts with light cues provided by the environment. Light, and specifically p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.007625
更新日期:2002-12-01 00:00:00
abstract::A detailed examination was conducted on the linear, or first-order kinetic component for K(+)((86)Rb(+)) influx into root segments of both low- and high-salt grown corn seedlings (Zea mays [A632 x Oh 43]). In tissue from both low- and high-salt grown roots, replacement of Cl(-) in the uptake solution by either SO(4) (...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.79.3.771
更新日期:1985-11-01 00:00:00
abstract::The soluble proteins of C(3) and C(4) mesophyll chloroplasts and C(4) bundle sheath extracts have been analyzed by gel electrophoresis for fraction I protein. Gel scans of soluble protein from C(4) bundle sheath extracts and C(3) mesophyll chloroplasts showed typical fraction I protein peaks that could be identified b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.57.5.730
更新日期:1976-05-01 00:00:00
abstract::A toxic effect of alpha,alpha-trehalose in an angiospermic plant, Cuscuta reflexa (dodder), is described. This disaccharide and its analogs, 2-aminotrehalose and 4-aminotrehalose, induced a rapid blackening of the terminal region of the vine which is involved in elongation growth. From the results of in vitro growth o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.6.1369
更新日期:1981-12-01 00:00:00