Membrane rupture is the common cause of damage to chloroplast membranes in leaves injured by freezing or excessive wilting.

Abstract:

:The effects of freezing and desiccation of spinach leaves (Spinacia oleracea L. cv Yates) on the thylakoid membranes were assessed using antibodies specific for thylakoid membrane proteins. The peripheral part of the chloroplast coupling factor ATPase (CF1) was used as a molecular marker for chemical membrane damage by chaotropic solutes. Plastocyanin, a soluble protein localized inside the closed thylakoid membrane system, was a marker for damage by mechanical membrane rupture. After freezing and wilting of leaves which resulted in damage, very little CF1 was detached from the membranes, whereas almost all plastocyanin was released from the thylakoids. It is suggested that in vivo dehydration both by freezing and desiccation results in membrane rupture rather than in the dissociation of peripheral thylakoid membrane proteins.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Hincha DK,Höfner R,Schwab KB,Heber U,Schmitt JM

doi

10.1104/pp.83.2.251

subject

Has Abstract

pub_date

1987-02-01 00:00:00

pages

251-3

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

83

pub_type

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