Overexpression of acyl carrier protein-1 alters fatty acid composition of leaf tissue in Arabidopsis.

Abstract:

:Acyl carrier protein (ACP) is a small (9 kD) acidic protein that is an essential cofactor in plant fatty acid biosynthesis. Most plants have several isoforms of ACP, some of which are expressed constitutively and others that appear to be more tissue specific. Although the critical role of ACP in fatty acid biosynthesis has been established, the role of the diverse number of isoforms has yet to be elucidated. We have generated transgenic Arabidopsis plants that express high levels of ACP-1, a seed-predominant ACP isoform, in leaf tissue under control of the cauliflower mosaic virus 35S promoter. Western and northern analysis of these plants demonstrate 3- to 8-fold increased expression of this isoform in leaf tissue, but no significant changes in seed. Analysis of the fatty acid composition of leaf tissue revealed that overexpression of ACP-1 in leaf tissue alters fatty acid composition. Significant decreases in levels of 16:3 were noted along with increases in 18:3. These findings represent the first in vivo report that overexpression of an ACP isoform results in changes in fatty acid composition in plants.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Branen JK,Chiou TJ,Engeseth NJ

doi

10.1104/pp.127.1.222

subject

Has Abstract

pub_date

2001-09-01 00:00:00

pages

222-9

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

127

pub_type

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