Abstract:
:To identify genetic loci involved in the regulation of organ-specific enzyme activities, a specific histochemical staining protocol was used in combination with quantitative trait locus (QTL) analysis. Using phosphoglucomutase (PGM) as an example, it is shown that enzyme activity can specifically, and with high resolution, be visualized in non-sectioned seedlings of Arabidopsis. The intensities of staining were converted to quantitative data and used as trait for QTL analysis using Landsberg erecta x Cape Verde Islands recombinant inbred lines. Independently, PGM activities were quantified in whole-seedling extracts, and these data were also used for QTL analysis. On the basis of extract data, six significant (P < 0.05) loci affecting PGM activity were found. From the histochemical data, one or more specific QTLs were found for each organ analyzed (cotyledons, shoot apex, hypocotyl, root, root neck, root tip, and root hairs). Loci detected for PGM activity in extracts colocated with loci for histochemical staining. QTLs were found coinciding with positions of (putative) PGM genes but also at other positions, the latter ones supposedly pointing toward regulatory genes. Some of this type of loci were also organ specific. It is concluded that QTL analysis based on histochemical data is feasible and may reveal organ-specific loci involved in the regulation of metabolic pathways.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Sergeeva LI,Vonk J,Keurentjes JJ,van der Plas LH,Koornneef M,Vreugdenhil Ddoi
10.1104/pp.103.027615subject
Has Abstractpub_date
2004-01-01 00:00:00pages
237-45issue
1eissn
0032-0889issn
1532-2548pii
pp.103.027615journal_volume
134pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Previous investigations suggested that specific auxin spatial distribution due to auxin movements to particular embryonic regions was important for normal embryonic pattern formation. To gain information on the molecular mechanism(s) by which auxin acts to direct pattern formation in specific embryonic regions, the ro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013466
更新日期:2003-03-01 00:00:00
abstract::The endoplasmic reticulum (ER) stress response is a signal transduction pathway activated by the perturbation of normal ER metabolism. We used the maize (Zea mays) floury-2 (fl2) mutant and soybean (Glycine max) suspension cultures treated with tunicamycin (Tm) to investigate the ER stress response as it relates to ph...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.1.267
更新日期:2001-05-01 00:00:00
abstract::While the metabolic networks in developing seeds during the period of reserve accumulation have been extensively characterized, much less is known about those present during seed desiccation and subsequent germination. Here we utilized metabolite profiling, in conjunction with selective mRNA and physiological profilin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.086694
更新日期:2006-11-01 00:00:00
abstract::Ascorbic acid oxidase activity in Myrothecium verrucaria extracts resulted in O(2) uptake exceeding 0.5 mole per mole of ascorbic acid and in CO(2) evolution. Measurement of oxidized ascorbic acid at completion of the reaction demonstrated that an average of 10% of the oxidized product disappeared. A comparison of the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.10.1553
更新日期:1966-12-01 00:00:00
abstract::The growth regulator abscisic acid (ABA) can be used to induce dormant bud structures (turions) in the duckweed Spirodela polyrrhiza L. In this paper we show that during this process, ABA rapidly induces elevated levels of mRNA transcripts encoding a novel basic peroxidase. In addition, we show that in the presence of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.2.497
更新日期:1994-06-01 00:00:00
abstract::Meristematic tissues from rye (Secale cereale) and oat (Avena sativa) were studied in an isothermal calorimeter at -3 degrees C. When the frozen tissue was placed in the calorimeter, the pressure increased within 4 d to 25 and 9 kPa above ambient pressure in the sample vessels containing crowns of rye and oat, respect...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.3.861
更新日期:2000-03-01 00:00:00
abstract::Histidine (His) is an essential ingredient for protein synthesis and is required by all living organisms. In higher plants, although there is considerable evidence that His is essential for plant growth and survival, there is very little information as to whether it plays any specific role in plant development. Here, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.084178
更新日期:2006-08-01 00:00:00
abstract::In all eukaryotes, cell cycle progression is controlled by cyclin-dependent kinases (CDKs) whose activity is regulated at several levels including inhibition by CDK inhibitors. Here, we report a comparative molecular and functional analysis of the tobacco (Nicotiana tomentosiformis) CDK inhibitor, NtKIS1a, and its spl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.008573
更新日期:2002-12-01 00:00:00
abstract::Annexins are a multigene family of calcium-dependent membrane-binding proteins that play important roles in plant cell signaling. Annexins are multifunctional proteins, and their function in plants is not comprehensively understood. Arabidopsis (Arabidopsis thaliana) annexins ANN1 and ANN2 are 64% identical in their p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00713
更新日期:2018-09-01 00:00:00
abstract::Hybrids between the C(4)-like species, Flaveria brownii, A. M. Powell and the C(3)-C(4) intermediate species Flaveria linearis Lag., Flaveria floridana Johnston, and Flaveria oppositifolia (DC.) Rydb. exhibited bivalent chromosome pairing during meiosis and stainability of pollen was high, ranging from 51 to 95%. An F...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.3.839
更新日期:1989-03-01 00:00:00
abstract::Our objective was to determine the sensitivity of components of the photosynthetic apparatus of maize (Zea mays), a C4 plant, to high temperature stress. Net photosynthesis (Pn) was inhibited at leaf temperatures above 38 degrees C, and the inhibition was much more severe when the temperature was increased rapidly rat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.002170
更新日期:2002-08-01 00:00:00
abstract::Cytokinins promote expansion of cotyledons detached from seedlings of more than a dozen species. The zeatin-enhanced expansion of cucumber (Cucumis sativus L. cv Marketer) cotyledons was investigated. In addition, whether acid secretion is involved in wall loosening accompanying such accelerated growth was evaluated. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.5.1470
更新日期:1982-11-01 00:00:00
abstract::Aroma contributes to the unique flavors of fruits and is important for fruit quality evaluation. Among the many volatiles in peach (Prunus persica) fruits, γ-decalactone has the greatest contribution to the characteristic peach aroma. Some peach cultivars have γ-decalactone contents that are too low to detect. Compari...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.00964
更新日期:2020-04-01 00:00:00
abstract::We observed the biphasic production of ethylene and reactive oxygen species (ROS) in susceptible tobacco (Nicotiana tabacum 'Wisconsin 38') plants after shoot inoculation with Phytophthora parasitica var nicotianae. The initial transient increase in ROS and ethylene at 1 and 3 h (phase I), respectively, was followed b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.194654
更新日期:2012-05-01 00:00:00
abstract::A procedure was developed to separate and partially purify two NAD(P)H dehydrogenases from the inner membrane of cauliflower (Brassica oleracea L.) mitochondria. The procedure used Triton X-100 extraction followed by (NH(4))(2)SO(4) precipitation and gel filtration (Sepharose G-200 column) chromatography. The first de...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.2.436
更新日期:1984-10-01 00:00:00
abstract::The levels and types of phospholipids changed in distinct phases during the germination of (32)P-labeled uredospores of Uromyces phaseoli. During the first 20 minutes of germination, the phospholipid content dropped to 40% of the pregermination level. Between 2 and 3 hours, phospholipid levels increased to approximate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.48.6.735
更新日期:1971-12-01 00:00:00
abstract::Accumulating evidence indicates that there is a multiplicity of cytochrome P-450 enzymes in plants. These monooxygenases are implicated in the metabolism of sterols, terpenes, gibberellins, isoflavonoids, and xenobiotics. Evidence that cytochromes P-450 are involved in the detoxification of herbicides (chlorotoluron, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.3.669
更新日期:1991-07-01 00:00:00
abstract::The species Arundinella hirta L. posseses a striking variation of the leaf anatomy that is characteristic of C(4) grasses. In addition to a sheath of large, bright green cells around the vascular bundles, there are strands of large parenchyma cells which appear identical to the bundle sheath cells and which run parall...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.5.397
更新日期:1973-11-01 00:00:00
abstract::Cauliflower mosaic virus (CaMV) replicated in protoplasts and in inoculated leaves of the non-host, cotton (Gossypium hirsutum, L.). Protoplasts prepared from suspension-cultured cotton cells were infected by incubation with liposome-encapsulated CaMV virions. During a 1-week culture period the amount of CaMV nucleic ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.3.633
更新日期:1987-03-01 00:00:00
abstract::Allene oxide synthase (AOS; hydroperoxide dehydratase; EC 4.2.1.92) catalyzes the first step in the biosynthesis of jasmonic acid from lipoxygenase-derived hydroperoxides of free fatty acids. Using the AOS cDNA from tomato (Lycopersicon esculentum), in which the role of jasmonic acid in wound-induced defense gene acti...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.4.1335
更新日期:2000-04-01 00:00:00
abstract::Isolated corn (Zea mays L.) mitochondria were found to oxidize reduced nicotinamide adenine dinucleotide phosphate in a KCl reaction medium. This oxidation was dependent on the presence of calcium or phosphate or both. Strontium and manganese substituted for calcium, but magnesium or barium did not. The oxidation of N...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.3.353
更新日期:1972-03-01 00:00:00
abstract::Plasmalemma redox activity in the diatom Thalassiosira is competitively inhibited by antiserum prepared against algal nitrate reductase (NR), and fluorescent labeling experiments reveal the binding of NR antiserum to the cell surface. Furthermore, the external electron acceptor Cu bathophenanthroline disulfonate cause...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.1.143
更新日期:1988-05-01 00:00:00
abstract::It has been reported that hydroxyphenylethylamines, such as tyramine and octopamine, are toxic to tobacco (Nicotiana tabacum L.) callus cultures grown in the presence of auxins, whereas calli grown in the presence of cytokinins and crown gall cultures are resistant to these amines (P. Christou and K.A. Barton [1989] P...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.2.329
更新日期:1993-10-01 00:00:00
abstract::Apple (Malus × domestica) trees naturally produce an excess of fruitlets that negatively affect the commercial value of fruits brought to maturity and impact their capacity to develop flower buds the following season. Therefore, chemical thinning has become an important cultural practice, allowing the selective remova...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.236117
更新日期:2014-04-01 00:00:00
abstract::The concentration-dependent stimulatory and inhibitory effect of N-glycans on tomato (Lycopersicon esculentum Mill.) fruit ripening was recently reported (B. Priem and K.C. Gross [1992] Plant Physiol 98: 399-401). We report here the structure of 10 free N-glycans in mature green tomatoes. N-Glycans were purified from ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.2.445
更新日期:1993-06-01 00:00:00
abstract::Fifty-four strains of Bradyrhizobium sp. (Lupinus) from worldwide collections were screened by a colony hybridization method for the presence of DNA sequences homologous to the structural genes of the Bradyrhizobium japonicum hydrogenase. Twelve strains exhibited strong colony hybridization signals, and subsequent Sou...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.1.78
更新日期:1989-01-01 00:00:00
abstract::Barley (Hordeum vulgare L. cv Halcyon) seedlings which had been grown in full strength complete inorganic nutrient media (containing 6 millimolar K(+)) had high internal K(+) concentrations and low values of K(+) ((86)Rb(+)) influx when influx was measured from solutions containing 100 micromolar K(+). Transfer of the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.92.4.1128
更新日期:1990-04-01 00:00:00
abstract::Spectral scanning was used to provide estimates of the leakage of the cyanogenic glucoside, dhurrin (p-hydroxy-[S]-mandelonitrile-beta-d-glucoside), and its metabolite, p-hydroxybenzaldehyde (p-HB), from young light-grown shoots of Atlas sorghum (Sorghum bicolor [L.] Moench) when these shoots were immersed in water, t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.3.869
更新日期:1984-07-01 00:00:00
abstract::Researchers have long endeavored to produce modified fatty acids in easily managed crop plants where they are not natively found. An important step toward this goal has been the biosynthesis of these valuable products in model oilseeds. The successful production of such fatty acids has revealed barriers to the broad a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01805
更新日期:2018-06-01 00:00:00
abstract::A phosphatidylinositol-specific phospholipase C of plant stems (EC 3.1.4.10) assayed at pH 6.6 and at 30 degrees C cleaved phosphatidylinositol such that more than 85% of the product was inositol-1-phosphate. Other phospholipids were cleaved 5 to 10% or less under these conditions. The phospholipase had both a soluble...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.4.1151
更新日期:1987-12-01 00:00:00