Replication of cauliflower mosaic virus DNA in leaves and suspension culture protoplasts of cotton.

Abstract:

:Cauliflower mosaic virus (CaMV) replicated in protoplasts and in inoculated leaves of the non-host, cotton (Gossypium hirsutum, L.). Protoplasts prepared from suspension-cultured cotton cells were infected by incubation with liposome-encapsulated CaMV virions. During a 1-week culture period the amount of CaMV nucleic acid as detected by nucleic acid hybridization in the protoplasts increased significantly regardless of whether or not the protoplasts contained vacuoles. In leaves inoculated with CaMV virions or CaMV DNA, viral DNA sequences were found by leaf skeleton hybridization to be located in small circular areas. DNA extracted from ultracentrifugal pellets of homogenates of inoculated leaves contained circular, gapped CaMV DNA only when inocula contained CaMV virions, CaMV DNA, or partial nested dimer CaMV plasmid DNA. When plants had been heavily watered, the CaMV DNA recovered contained degraded CaMV DNA. The results suggest that the host range limitation for CaMV is not due to an inability to replicate or spread locally in inoculated leaves.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Hussain MM,Melcher U,Whittle T,Williams A,Brannan CM,Mitchell ED

doi

10.1104/pp.83.3.633

subject

Has Abstract

pub_date

1987-03-01 00:00:00

pages

633-9

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

83

pub_type

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