Isolation of Intact and Functional Chloroplasts from Mesophyll and Bundle Sheath Protoplasts of the C(4) Plant Panicum miliaceum.

abstract：:The molecular weight and isoelectric point of the plasma membrane H(+)-ATPase from red beet storage tissue were determined using N,N'-dicyclohexylcarbodiimide (DCCD) and a H(+)-ATPase antibody. When plasma membrane vesicles were incubated with 20 micromolar [(14)C]-DCCD at 0 degrees C, a single 97,000 dalton protein w...

journal_title：Plant physiology

authors： Oleski NA,Bennett AB

更新日期：1987-03-01 00:00:00

abstract：:Rice (Oryza sativa) is the staple food for over half the world's population yet may represent a significant dietary source of inorganic arsenic (As), a nonthreshold, class 1 human carcinogen. Rice grain As is dominated by the inorganic species, and the organic species dimethylarsinic acid (DMA). To investigate how As ...

journal_title：Plant physiology

authors： Carey AM,Scheckel KG,Lombi E,Newville M,Choi Y,Norton GJ,Charnock JM,Feldmann J,Price AH,Meharg AA

更新日期：2010-01-01 00:00:00

abstract：:A study of the distribution of lectins over different vegetative tissues of barley (Hordeum vulgare L.) plants, which were grown under normal crop conditions, indicated that lectin occurs in roots, leaves, and developing ears. Isolation and characterization of both root and leaf lectins led to the conclusions (a) that...

journal_title：Plant physiology

authors： Cammue B,Stinissen HM,Peumans WJ

更新日期：1985-06-01 00:00:00

abstract：:As part of a more detailed study on plant tumorigenesis, the action of gibberellic acid (GA(3)) in wounded potato tuber tissues as a model system has been evaluated. GA(3) stimulates total RNA synthesis in wounded tissues, the optimal concentration being 0.1 micromolar. The responsiveness of the tissue toward the horm...

journal_title：Plant physiology

authors： Wielgat B

更新日期：1979-11-01 00:00:00

abstract：:While the importance of cell type specificity in plant adaptive responses is widely accepted, only a limited number of studies have addressed this issue at the functional level. We have combined electrophysiological, imaging, and biochemical techniques to reveal the physiological mechanisms conferring higher sensitivi...

journal_title：Plant physiology

authors： Shabala L,Zhang J,Pottosin I,Bose J,Zhu M,Fuglsang AT,Velarde-Buendia A,Massart A,Hill CB,Roessner U,Bacic A,Wu H,Azzarello E,Pandolfi C,Zhou M,Poschenrieder C,Mancuso S,Shabala S

更新日期：2016-12-01 00:00:00

abstract：:Traditional virus-induced gene silencing (VIGS) is a powerful virus-based short interfering RNA-mediated RNA silencing technique for plant functional genomics. Besides short interfering RNAs, microRNAs (miRNAs) have also been shown to regulate gene expression by RNA silencing in various organisms. However, plant virus...

journal_title：Plant physiology

authors： Tang Y,Wang F,Zhao J,Xie K,Hong Y,Liu Y

更新日期：2010-06-01 00:00:00

abstract：:Relative elemental growth rate (REGR) profiles describe spatial patterns of growth intensity; they are indispensable for causal growth analyses. Published methods of REGR profile determination from marking experiments fall in two classes: the profile is either described by a series of segmental growth rates, or calcul...

journal_title：Plant physiology

authors： Peters WS,Bernstein N

更新日期：1997-04-01 00:00:00

abstract：:Maximizing light capture by light-harvesting pigment optimization represents an attractive but challenging strategy to improve photosynthetic efficiency. Here, we report that loss of a previously uncharacterized gene, HIGH PHOTOSYNTHETIC EFFICIENCY1 (HPE1), optimizes light-harvesting pigments, leading to improved phot...

journal_title：Plant physiology

authors： Jin H,Li M,Duan S,Fu M,Dong X,Liu B,Feng D,Wang J,Wang HB

更新日期：2016-11-01 00:00:00

abstract：:Lipid-linked oligosaccharides were synthesized with the particulate enzyme preparation from mung bean (Phaseolus aureus) seedlings in the presence of GDP-[(14)C] mannose. The oligosaccharides were released from the lipids by mild acid hydrolysis and purified by several passages on Biogel P-4 columns. Five different ol...

journal_title：Plant physiology

authors： Hori H,Elbein AD

更新日期：1982-07-01 00:00:00

abstract：:Cucumber (Cucumis sativus L., cv Poinsette) plants were sprayed with 20 millimolar 5-aminolevulinic acid and then incubated in the dark for 14 hours. The intact chloroplasts were isolated from the above plants in the dark and were exposed to weak light (250 micromoles per square meter per second). Within 30 minutes, p...

journal_title：Plant physiology

authors： Chakraborty N,Tripathy BC

更新日期：1992-01-01 00:00:00

abstract：:Northern blot hybridization analyzes revealed that poly(A(+)) RNAs homologous to eight heat shock (HS)-specific cDNA clones were induced by arsenite (As) or Cd treatments. The mRNAs accumulated slower, and maximum accumulations were consistently lower than HS-induced levels. Prolonged treatment with low concentrations...

journal_title：Plant physiology

authors： Edelman L,Czarnecka E,Key JL

更新日期：1988-04-01 00:00:00

abstract：:During the last century, two key hypotheses have been proposed to explain apical dominance in plants: auxin promotes the production of a second messenger that moves up into buds to repress their outgrowth, and auxin saturation in the stem inhibits auxin transport from buds, thereby inhibiting bud outgrowth. The recent...

journal_title：Plant physiology

authors： Brewer PB,Dun EA,Ferguson BJ,Rameau C,Beveridge CA

更新日期：2009-05-01 00:00:00

abstract：:Succulent stems of Cissus quadrangularis L. (Vitaceae) contain glutamine synthetase, glutamate synthase, and glutamate dehydrogenase. The CO(2) and water gas exchanges of detached internodes were typical for Crassulacean acid metabolism plants. During three physiological phases, e.g. in the dark, in the early illumina...

journal_title：Plant physiology

authors： Berger MG,Sprengart ML,Kusnan M,Fock HP

更新日期：1986-06-01 00:00:00

abstract：:Chlamydomonas reinhardtii adapts to the stress of CO(2)-limiting conditions through the induction of a set of genes including CAH1, which encodes a periplasmic carbonic anhydrase. CAH1 is up-regulated under low-CO(2) conditions (air containing 0.04% [v/v] CO(2)) in the presence of light, whereas it is down-regulated u...

journal_title：Plant physiology

authors： Kucho Ki,Ohyama K,Fukuzawa H

更新日期：1999-12-01 00:00:00

abstract：:High-throughput genotype screening is rapidly becoming a standard research tool in the post-genomic era. A major bottleneck currently exists, however, that limits the utility of this approach in the plant sciences. The rate-limiting step in current high-throughput pipelines is that tissue samples from living plants mu...

journal_title：Plant physiology

authors： Krysan P

更新日期：2004-07-01 00:00:00

abstract：:The gross tissue distribution, intracellular fate, and chemical behavior of Ni(2+) in soybean plants (Glycine max cv. Williams) were investigated. Following root absorption, Ni was highly mobile in the plant, with leaves being the major sink in the shoots for Ni during vegetative growth. A senescence >70% of the Ni pr...

journal_title：Plant physiology

authors： Cataldo DA,Garland TR,Wildung RE

更新日期：1978-10-01 00:00:00

abstract：:Here, we investigated the physiological role of Arabidopsis (Arabidopsis thaliana) AtNUDX6, the gene encoding ADP-ribose (Rib)/NADH pyrophosphohydrolase, using its overexpressor (Pro35S:AtNUDX6) or disruptant (KO-nudx6). The level of NADH in Pro35S:AtNUDX6 and KO-nudx6 plants was decreased and increased, respectively,...

journal_title：Plant physiology

authors： Ishikawa K,Yoshimura K,Harada K,Fukusaki E,Ogawa T,Tamoi M,Shigeoka S

更新日期：2010-04-01 00:00:00

abstract：:The citrate carrier from maize (Zea mays) shoot mitochondria was solubilized with Triton X-100 and purified by sequential chromatography on hydroxyapatite and hydroxyapatite/celite in the presence of cardiolipin. SDS-gel electrophoresis of the purified fraction showed a single polypeptide band with an apparent molecul...

journal_title：Plant physiology

authors： Genchi G,Spagnoletta A,De Santis A,Stefanizzi L,Palmieri F

更新日期：1999-07-01 00:00:00

abstract：:The emergence of sterile individuals in the hybrid backcross progeny of wild and cultivated rice limits the use of wild rice alleles for improving cultivated rice, but the molecular mechanisms underlying this sterility remain unclear. Here, we identified the semisterile introgression line YIL42, derived from a cross b...

journal_title：Plant physiology

authors： Hou J,Cao C,Ruan Y,Deng Y,Liu Y,Zhang K,Tan L,Zhu Z,Cai H,Liu F,Sun H,Gu P,Sun C,Fu Y

更新日期：2019-05-01 00:00:00

abstract：:Phosphinothricin (PPT) is the active component of a family of environmentally safe, nonselective herbicides. Resistance to PPT in transgenic crops has been reported by nuclear expression of a bar transgene encoding phosphinothricin acetyltransferase, a detoxifying enzyme. We report here expression of a bacterial bar g...

journal_title：Plant physiology

authors： Lutz KA,Knapp JE,Maliga P

更新日期：2001-04-01 00:00:00

abstract：:Proplastids and prolamellar bodies with tubular membranes were isolated from the dark grown primary leaves of bean seedlings (Phaseolus vulgaris L.). The combination of fluorescence microscopy and negative contrast electron microscopy provided the tentative identification of protochlorophyll holochrome as a constituen...

journal_title：Plant physiology

authors： Kahn A

更新日期：1968-11-01 00:00:00

abstract：:The physiological and molecular bases for contrasting [alpha]-amylase phenotypes were examined in germinating seeds of two barley (Hordeum vulgare L.) cultivars, Morex and Steptoe. Morex is a high-quality malting barley that develops high [alpha]-amylase activity soon after germination. Steptoe is a feed barley that d...

journal_title：Plant physiology

authors： Skadsen RW

更新日期：1993-05-01 00:00:00

abstract：:Greenhouse and field studies examined the effect of flower or seedhead removal on leaf senescence and associated changes in sunflower (Helianthus annuus L.) plants. At intervals during seed development, selected leaves (leaves 6 through 8 from the top in the greenhouse and leaf 7 from the top in the field) were harves...

journal_title：Plant physiology

authors： Ho I,Below FE,Hageman RH

更新日期：1987-04-01 00:00:00

abstract：:Oligomeric proanthocyanidins (PAs) composed primarily of epicatechin units accumulate in the seed coats of the model legume Medicago truncatula, reaching maximal levels at around 20 d after pollination. Genes encoding the single Medicago anthocyanidin synthase (ANS; EC 1.14.11.19) and leucoanthocyanidin reductase (LAR...

journal_title：Plant physiology

authors： Pang Y,Peel GJ,Wright E,Wang Z,Dixon RA

更新日期：2007-11-01 00:00:00

abstract：:In the last few years, much attention has been given to the role of proteins that accumulate during water deficit. In this work, we analyzed the electrophoretic patterns of basic protein extracts, enriched for a number of cell-wall proteins, from bean (Phaseolus vulgaris L.) seedlings and 21-d-old plants subjected to ...

journal_title：Plant physiology

authors： Covarrubias AA,Ayala JW,Reyes JL,Hernandez M,Garciarrubio A

更新日期：1995-04-01 00:00:00

abstract：:Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of the starchy endosperm protein of rice (Oryza sativa L. Japonica cv Koshihikari) during seed development confirmed that storage protein begins to accumulate about 5 days after flowering. Two polypeptide groups, 22 to 23 and 37 to 39 kilodaltons, the ...

journal_title：Plant physiology

authors： Yamagata H,Sugimoto T,Tanaka K,Kasai Z

更新日期：1982-10-01 00:00:00

abstract：:A water-soluble chlorophyll protein (WSCP) in cauliflower (Brassica oleracea L.) was purified and its N-terminal sequence was determined. Forty-six of 48 residues of the sequence completely matched those of the drought-induced 22-kD protein (BnD22) in rapeseed (Brassica napus L.). All 40 sequenced residues of WSCP fro...

journal_title：Plant physiology

authors： Nishio N,Satoh H

更新日期：1997-10-01 00:00:00

abstract：:Starch biosynthetic enzymes from maize (Zea mays) and wheat (Triticum aestivum) amyloplasts exist in cell extracts in high molecular weight complexes; however, the nature of those assemblies remains to be defined. This study tested the interdependence of the maize enzymes starch synthase IIa (SSIIa), SSIII, starch bra...

journal_title：Plant physiology

authors： Hennen-Bierwagen TA,Lin Q,Grimaud F,Planchot V,Keeling PL,James MG,Myers AM

更新日期：2009-03-01 00:00:00

abstract：:The effects of drought on Photosystem II (PSII) fluorescence and photosynthetic electron transport activities were analyzed in cotton. Water stress did not modify the amplitude of leaf variable fluorescence at room temperature in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) nor at 77 K. It is therefo...

journal_title：Plant physiology

authors： Genty B,Briantais JM,Da Silva JB

更新日期：1987-02-01 00:00:00

abstract：:The vascular cambium produces secondary xylem and phloem in plants and is responsible for wood formation in forest trees. In this study we used a microscale mass-spectrometry technique coupled with cryosectioning to visualize the radial concentration gradient of endogenous indole-3-acetic acid (IAA) across the cambial...

journal_title：Plant physiology

authors： Uggla C,Mellerowicz EJ,Sundberg B

更新日期：1998-05-01 00:00:00