Making Epidermal Bladder Cells Bigger: Developmental- and Salinity-Induced Endopolyploidy in a Model Halophyte.

Abstract:

:Endopolyploidy occurs when DNA replication takes place without subsequent mitotic nuclear division, resulting in cell-specific ploidy levels within tissues. In plants, endopolyploidy plays an important role in sustaining growth and development, but only a few studies have demonstrated a role in abiotic stress response. In this study, we investigated the function of ploidy level and nuclear and cell size in leaf expansion throughout development and tracked cell type-specific ploidy in the halophyte Mesembryanthemum crystallinum In addition to developmental endopolyploidy, we examined the effects of salinity stress on ploidy level. We focused specifically on epidermal bladder cells (EBC), which are modified balloon-like trichomes, due to their large size and role in salt accumulation. Our results demonstrate that ploidy increases as the leaves expand in a similar manner for each leaf type, and ploidy levels up to 512C were recorded for nuclei in EBC of leaves of adult plants. Salt treatment led to a significant increase in ploidy levels in the EBC, and these cells showed spatially related differences in their ploidy and nuclear and cell size depending on the positions on the leaf and stem surface. Transcriptome analysis highlighted salinity-induced changes in genes involved in DNA replication, cell cycle, endoreduplication, and trichome development in EBC. The increase in cell size and ploidy observed in M. crystallinum under salinity stress may contribute to salt tolerance by increasing the storage capacity for sodium sequestration brought about by higher metabolic activity driving rapid cell enlargement in the leaf tissue and EBC.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Barkla BJ,Rhodes T,Tran KT,Wijesinghege C,Larkin JC,Dassanayake M

doi

10.1104/pp.18.00033

subject

Has Abstract

pub_date

2018-06-01 00:00:00

pages

615-632

issue

2

eissn

0032-0889

issn

1532-2548

pii

pp.18.00033

journal_volume

177

pub_type

杂志文章
  • Biosynthesis of delta-Aminolevulinic Acid in Chlamydomonas reinhardtii: Study of the Transamination Mechanism Using Specifically Labeled Glutamate.

    abstract::The first committed intermediate of chlorophyll biosynthesis, delta-aminolevulinic acid (ALA), is synthesized from glutamate in the plant cell. The last step of ALA synthesis is a transamination reaction which converts glutamate-1-semialdehyde (GSA) to ALA. The mechanism of the transamination was examined by using glu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.3.793

    authors: Mau YH,Wang WY

    更新日期:1988-03-01 00:00:00

  • Metabolism of sugars in the endosperm of developing seeds of oilseed rape.

    abstract::The sugars in the endosperm of a developing seed have many potential roles, including the supply of carbon to the developing embryo and controlling gene expression in it. Our understanding of their metabolism is, however, fragmentary and is confined to a very few species (especially Vicia spp.). To develop a quantitat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010868

    authors: Hill LM,Morley-Smith ER,Rawsthorne S

    更新日期:2003-01-01 00:00:00

  • An mRNA putatively coding for an O-methyltransferase accumulates preferentially in maize roots and is located predominantly in the region of the endodermis.

    abstract::ZRP4, a 1.4-kb mRNA that preferentially accumulates in roots of young Zea mays L. plants, was identified by isolation of the corresponding cDNA clone. Genomic Southern analysis indicates that the zrp4 gene is represented once in the corn genome. The deduced ZRP4 polypeptide of 39,558 D is rich in leucine, serine, and ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.3.1001

    authors: Held BM,Wang H,John I,Wurtele ES,Colbert JT

    更新日期:1993-07-01 00:00:00

  • Manipulation of galactolipid Fatty Acid composition with substituted pyridazinones.

    abstract::The fatty acid composition of the major lipids of the chloroplast membranes, the mono- and digalactosyl diglycerides, can be definably altered with various substituted pyridazinones. Galactolipid fatty acid composition of wheat (Triticum aestivum L.) can be altered so that there is a decrease in linolenic acid accompa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.1.38

    authors: John JB

    更新日期:1976-01-01 00:00:00

  • Superoxide Dismutase: A POSSIBLE PROTECTIVE ENZYME AGAINST OZONE INJURY IN SNAP BEANS (PHASEOLUS VULGARIS L.).

    abstract::An experimental chemical N-[2-(2-oxo-1-imidazolidinyl)ethyl]-N'-phenylurea (EDU), is an effective protectant against acute and chronic foliar injury due to ozone (0(3)) when sprayed on intact leaves or supplied to the plants through soil application. An 0(3)-sensitive snap bean cultivar (Phaseolus vulgaris L. ;Bush Bl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.6.1444

    authors: Lee EH,Bennett JH

    更新日期:1982-06-01 00:00:00

  • Characterization of Plasma Membrane-associated Adenosine Triphosphase Activity of Oat Roots.

    abstract::ATPase activity of plasma membranes isolated from oat (Avena sativa L. cv. Goodfield) roots was activated by divalent cations (Mg(2+) = Mn(2+) > Zn(2+) > Fe(2+) > Ca(2+)) and further stimulated by KCl and a variety of monovalent salts, both inorganic and organic. The enzyme exhibited greater specificity for cations th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.1.6

    authors: Leonard RT,Hodges TK

    更新日期:1973-07-01 00:00:00

  • Relative activities of NAD- and NADP- isocritric dehydrogenases in bean mitochondria modified by glycerol or NADP.

    abstract::Mitochondria from cotyledons of Vigna sesquipedalis (L.) Fruwirth (starchy seed) showed no NAD-isocitric dehydrogenase (NAD-IDH) activity by the methods which have been known to be useful for the detection of NAD-IDH in mitochondria of plants including castor bean and alaska pea. When the Vigna cotyledon mitochondria ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.2.262

    authors: Yamamoto Y

    更新日期:1969-02-01 00:00:00

  • Directed mutation of the Rubisco large subunit of tobacco influences photorespiration and growth.

    abstract::The gene for the large subunit of Rubisco was specifically mutated by transforming the chloroplast genome of tobacco (Nicotiana tabacum). Codon 335 was altered to encode valine instead of leucine. The resulting mutant plants could not grow without atmospheric CO2 enrichment. In 0.3% (v/v) CO2, the mutant and wild-type...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.121.2.579

    authors: Whitney SM,von Caemmerer S,Hudson GS,Andrews TJ

    更新日期:1999-10-01 00:00:00

  • Arabidopsis HMA2, a divalent heavy metal-transporting P(IB)-type ATPase, is involved in cytoplasmic Zn2+ homeostasis.

    abstract::PIB-type ATPases transport heavy metal ions (Cu+, Cu2+, Zn2+, Cd2+, Co2+, etc.) across biological membranes. Several members of this subfamily are present in plants. Higher plants are the only eukaryotes where putative Zn(2+)-ATPases have been identified. We have cloned HMA2, a PIB-ATPase present in Arabidopsis (Arabi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.046292

    authors: Eren E,Argüello JM

    更新日期:2004-11-01 00:00:00

  • Effect of Low Root Medium pH on Net Proton Release, Root Respiration, and Root Growth of Corn (Zea mays L.) and Broad Bean (Vicia faba L.).

    abstract::The effect of low pH on net H(+) release and root growth of corn (Zea mays L.) and broad bean (Vicia faba L.) seedlings was investigated in short-term experiments at constant pH. Broad bean was more sensitive to low pH than corn: the critical values (pH values below which net H(+) release and root growth ceased) were ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.2.415

    authors: Yan F,Schubert S,Mengel K

    更新日期:1992-06-01 00:00:00

  • The role of the plant-specific ALTERED XYLOGLUCAN9 protein in Arabidopsis cell wall polysaccharide O-acetylation.

    abstract::A mutation in the ALTERED XYLOGLUCAN9 (AXY9) gene was found to be causative for the decreased xyloglucan acetylation phenotype of the axy9.1 mutant, which was identified in a forward genetic screen for Arabidopsis (Arabidopsis thaliana) mutants. The axy9.1 mutant also exhibits decreased O-acetylation of xylan, implyin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.256479

    authors: Schultink A,Naylor D,Dama M,Pauly M

    更新日期:2015-04-01 00:00:00

  • ROS1-Dependent DNA Demethylation Is Required for ABA-Inducible NIC3 Expression.

    abstract::DNA methylation plays an important role in diverse developmental processes in many eukaryotes, including the response to environmental stress. Abscisic acid (ABA) is a plant hormone that is up-regulated under stress. The involvement of DNA methylation in the ABA response has been reported but is poorly understood. DNA...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01471

    authors: Kim JS,Lim JY,Shin H,Kim BG,Yoo SD,Kim WT,Huh JH

    更新日期:2019-04-01 00:00:00

  • Characterization of MdMADS2, a member of the SQUAMOSA subfamily of genes, in apple.

    abstract::A MADS-box gene, MdMADS2, was isolated from the apple (Malus x domestica Borkh.) var Fuji and its developmental expression pattern was studied during flower development. MdMADS2 shares a high degree of amino acid sequence identity with the SQUAMOSA subfamily of genes. RNA blot analysis showed that MdMADS2 is transcrib...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.4.969

    authors: Sung SK,Yu GH,An G

    更新日期:1999-08-01 00:00:00

  • Ethylene insensitivity conferred by the Green-ripe and Never-ripe 2 ripening mutants of tomato.

    abstract::The ripening of a fleshy fruit represents the summation of an array of biochemical processes that are regulated by interactions between developmental programs and environmental inputs. Analysis of tomato (Solanum lycopersicum) mutants and inhibitor studies indicate that ethylene is necessary for full development of th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.057745

    authors: Barry CS,McQuinn RP,Thompson AJ,Seymour GB,Grierson D,Giovannoni JJ

    更新日期:2005-05-01 00:00:00

  • Protoplasts surviving freezing to -196 C and osmotic dehydration in 5 molar salt solutions prepared from the bark of winter black locust trees.

    abstract::Free protoplasts were prepared from the living bark tissue of the trunk of summer and winter black locust trees by enzymic digestion of thin slices of the tissue for 3 hours in a medium containing 2% Onozuka cellulase, 2% Rhozyme pectinase, and 2% Driselase in mannitol solutions using 0.4 molar mannitol for summer tis...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.4.722

    authors: Siminovitch D

    更新日期:1979-04-01 00:00:00

  • Metabolism of C-Maltose in Avena fatua Seeds During Germination.

    abstract::Non-dormant and dormant seeds of Avena fatua metabolize (14)C-maltose in different ways: in non-dormant seeds, (14)C-maltose administered to the endosperm is readily converted to sucrose in the scutellum and translocated to the embryo; in dormant seeds, little sucrose is synthesized from (14)C-maltose, and maltose and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.5.770

    authors: Chen SS,Varner JE

    更新日期:1969-05-01 00:00:00

  • The G-Box Transcriptional Regulatory Code in Arabidopsis.

    abstract::Plants have significantly more transcription factor (TF) families than animals and fungi, and plant TF families tend to contain more genes; these expansions are linked to adaptation to environmental stressors. Many TF family members bind to similar or identical sequence motifs, such as G-boxes (CACGTG), so it is diffi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01086

    authors: Ezer D,Shepherd SJK,Brestovitsky A,Dickinson P,Cortijo S,Charoensawan V,Box MS,Biswas S,Jaeger KE,Wigge PA

    更新日期:2017-10-01 00:00:00

  • Analysis of detergent-resistant membranes in Arabidopsis. Evidence for plasma membrane lipid rafts.

    abstract::The trafficking and function of cell surface proteins in eukaryotic cells may require association with detergent-resistant sphingolipid- and sterol-rich membrane domains. The aim of this work was to obtain evidence for lipid domain phenomena in plant membranes. A protocol to prepare Triton X-100 detergent-resistant me...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.053041

    authors: Borner GH,Sherrier DJ,Weimar T,Michaelson LV,Hawkins ND,Macaskill A,Napier JA,Beale MH,Lilley KS,Dupree P

    更新日期:2005-01-01 00:00:00

  • Relationship between CO2 Assimilation, Photosynthetic Electron Transport, and Active O2 Metabolism in Leaves of Maize in the Field during Periods of Low Temperature

    abstract::Measurements of the quantum efficiencies of photosynthetic electron transport through photosystem II (phiPSII) and CO2 assimilation (phiCO2) were made simultaneously on leaves of maize (Zea mays) crops in the United Kingdom during the early growing season, when chilling conditions were experienced. The activities of a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.2.571

    authors: Fryer MJ,Andrews JR,Oxborough K,Blowers DA,Baker NR

    更新日期:1998-02-01 00:00:00

  • Cyclopropane Fatty acids in relation to earliness in spring and drought tolerance in plants.

    abstract::Long chain cyclopropane fatty acids were observed in the sulfolipid fraction extracted from leaves of the early spring plants Galanthus nivalis L. and Anthriscus silvestris L. (Hoffm.). The content of cyclopropane fatty acids with 25 carbon atoms appeared to be clearly correlated with earliness in spring, and it range...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.3.307

    authors: Kuiper PJ,Stuiver B

    更新日期:1972-03-01 00:00:00

  • The Arabidopsis family GT43 glycosyltransferases form two functionally nonredundant groups essential for the elongation of glucuronoxylan backbone.

    abstract::There exist four members of family GT43 glycosyltransferases in the Arabidopsis (Arabidopsis thaliana) genome, and mutations of two of them, IRX9 and IRX14, have previously been shown to cause a defect in glucuronoxylan (GX) biosynthesis. However, it is currently unknown whether IRX9 and IRX14 perform the same biochem...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.155309

    authors: Lee C,Teng Q,Huang W,Zhong R,Ye ZH

    更新日期:2010-06-01 00:00:00

  • Changes in Chloroplast Membrane Lipids during Adaptation of Barley to Extreme Salinity.

    abstract::During adaptation of barley (Hordeum vulgare L.) seedlings to extremely high concentrations of sodium chloride in the root space, the content of galactolipids of chloroplast membranes decreased considerably. Alterations in membrane lipids were due to the high concentration of ions rather than to the increase in the wa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.3.326

    authors: Müller M,Santarius KA

    更新日期:1978-09-01 00:00:00

  • Altered nitrogenous pools induced by the azolla-anabaena azolla symbiosis.

    abstract::The free amino acid and ammonia pools of Azolla caroliniana were analyzed by quantitative column chromatography on columns capable of separating all of the nitrogenous constituents normally found in physiological fluids. Comparisons were made of plants containing symbiotic algae and grown on nitrogen-free media, plant...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.6.798

    authors: Newton JW,Cavins JF

    更新日期:1976-12-01 00:00:00

  • Ectopic expression of a horseradish peroxidase enhances growth rate and increases oxidative stress resistance in hybrid aspen.

    abstract::We previously demonstrated that overexpression of the horseradish (Armoracia rusticana) peroxidase prxC1a gene stimulated the growth rate of tobacco (Nicotiana tabacum) plants. Here, the cauliflower mosaic virus 35S::prxC1a construct was introduced into hybrid aspen (Populus sieboldii x Populus grandidentata). The gro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.019794

    authors: Kawaoka A,Matsunaga E,Endo S,Kondo S,Yoshida K,Shinmyo A,Ebinuma H

    更新日期:2003-07-01 00:00:00

  • (1-->3)-beta-d-Glucan Synthase from Sugar Beet : I. Isolation and Solubilization.

    abstract::A (1-->3)-beta-glucan synthase has been isolated from petiole tissue of sugar beet (Beta vulgaris L.). Enzyme activity is associated with a membrane fraction with a density of 1.03 grams per cubic centimeter when subjected to isopycnic density gradient centrifugation in Percoll. The reaction product was determined to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.1.171

    authors: Morrow DL,Lucas WJ

    更新日期:1986-05-01 00:00:00

  • The Non-Mendelian Green Cotyledon Gene in Soybean Encodes a Small Subunit of Photosystem II.

    abstract::Chlorophyll degradation plays important roles in leaf senescence including regulation of degradation of chlorophyll-binding proteins. Although most genes encoding enzymes of the chlorophyll degradation pathway have been identified, the regulation of their activity has not been fully understood. Green cotyledon mutants...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01589

    authors: Kohzuma K,Sato Y,Ito H,Okuzaki A,Watanabe M,Kobayashi H,Nakano M,Yamatani H,Masuda Y,Nagashima Y,Fukuoka H,Yamada T,Kanazawa A,Kitamura K,Tabei Y,Ikeuchi M,Sakamoto W,Tanaka A,Kusaba M

    更新日期:2017-04-01 00:00:00

  • Ectopic expression of an amino acid transporter (VfAAP1) in seeds of Vicia narbonensis and pea increases storage proteins.

    abstract::Storage protein synthesis is dependent on available nitrogen in the seed, which may be controlled by amino acid import via specific transporters. To analyze their rate-limiting role for seed protein synthesis, a Vicia faba amino acid permease, VfAAP1, has been ectopically expressed in pea (Pisum sativum) and Vicia nar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.056523

    authors: Rolletschek H,Hosein F,Miranda M,Heim U,Götz KP,Schlereth A,Borisjuk L,Saalbach I,Wobus U,Weber H

    更新日期:2005-04-01 00:00:00

  • Regulation of light-harvesting chlorophyll protein biosynthesis in greening seedlings : a species comparison.

    abstract::The biosynthesis of the chlorophyll a/b binding protein associated with photosystem II (LHC-II) was characterized during light-induced greening of etiolated barley (Hordeum vulgare [L.] cv Boone), maize (Zea mays [L.] Pioneer 3148), pea (Pisum sativum [L.] cv Progress 9), and soybean (Glycine max [L.] Merr. cv Ransom ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.4.971

    authors: Mathis JN,Burkey KO

    更新日期:1987-12-01 00:00:00

  • Dehydration, water fluxes, and permeability of tobacco leaf tissue.

    abstract::Removal of the lower epidermis from a tobacco leaf allows a faster and wider range of water fluxes, without damaging the mesophyll. It also permits a more direct examination of the photosynthetic potential of the tissue at various levels of hydration.The rehydration rate of leaf discs is essentially linear. It decreas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.48.5.575

    authors: Graziani Y,Livne A

    更新日期:1971-11-01 00:00:00

  • A transglutaminase immunologically related to tissue transglutaminase catalyzes cross-linking of cell wall proteins in Chlamydomonas reinhardtii.

    abstract::The addition of primary amines to the growth medium of the unicellular green alga Chlamydomonas reinhardtii disrupts cell wall assembly in both vegetative and zygotic cells. Primary amines are competitive inhibitors of the protein-cross-linking activity of transglutaminases. Two independent assays for transglutaminase...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.121.3.1003

    authors: Waffenschmidt S,Kusch T,Woessner JP

    更新日期:1999-11-01 00:00:00