Biosynthesis of delta-Aminolevulinic Acid in Chlamydomonas reinhardtii: Study of the Transamination Mechanism Using Specifically Labeled Glutamate.

Abstract:

:The first committed intermediate of chlorophyll biosynthesis, delta-aminolevulinic acid (ALA), is synthesized from glutamate in the plant cell. The last step of ALA synthesis is a transamination reaction which converts glutamate-1-semialdehyde (GSA) to ALA. The mechanism of the transamination was examined by using glutamate, specifically labeled with either 1-(13)C or (15)N, as substrate for ALA synthesis. After incubating with crude enzymes extracted from Chlamydomonas reinhardtii, the distribution of labels in purified ALA molecules was examined by nuclear magnetic resonance spectroscopy and gas chromatography-mass spectrometry. We found that both isotopes were present in the same ALA molecule. We interpret the results to mean that intermolecular transamination occurs during the conversion of GSA to ALA.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Mau YH,Wang WY

doi

10.1104/pp.86.3.793

subject

Has Abstract

pub_date

1988-03-01 00:00:00

pages

793-7

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

86

pub_type

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