Abstract:
:The first committed intermediate of chlorophyll biosynthesis, delta-aminolevulinic acid (ALA), is synthesized from glutamate in the plant cell. The last step of ALA synthesis is a transamination reaction which converts glutamate-1-semialdehyde (GSA) to ALA. The mechanism of the transamination was examined by using glutamate, specifically labeled with either 1-(13)C or (15)N, as substrate for ALA synthesis. After incubating with crude enzymes extracted from Chlamydomonas reinhardtii, the distribution of labels in purified ALA molecules was examined by nuclear magnetic resonance spectroscopy and gas chromatography-mass spectrometry. We found that both isotopes were present in the same ALA molecule. We interpret the results to mean that intermolecular transamination occurs during the conversion of GSA to ALA.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Mau YH,Wang WYdoi
10.1104/pp.86.3.793subject
Has Abstractpub_date
1988-03-01 00:00:00pages
793-7issue
3eissn
0032-0889issn
1532-2548journal_volume
86pub_type
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