Variations in the composition of gelling agents affect morphophysiological and molecular responses to deficiencies of phosphate and other nutrients.

Abstract:

:Low inorganic phosphate (Pi) availability triggers an array of spatiotemporal adaptive responses in Arabidopsis (Arabidopsis thaliana). There are several reports on the effects of Pi deprivation on the root system that have been attributed to different growth conditions and/or inherent genetic variability. Here we show that the gelling agents, largely treated as inert components, significantly affect morphophysiological and molecular responses of the seedlings to deficiencies of Pi and other nutrients. Inductively coupled plasma-mass spectroscopy analysis revealed variable levels of elemental contaminants not only in different types of agar but also in different batches of the same agar. Fluctuating levels of phosphorus (P) in different agar types affected the growth of the seedlings under Pi-deprivation condition. Since P interacts with other elements such as iron, potassium, and sulfur, contaminating effects of these elements in different agars were also evident in the Pi-deficiency-induced morphological and molecular responses. P by itself acted as a contaminant when studying the responses of Arabidopsis to micronutrient (iron and zinc) deficiencies. Together, these results highlighted the likelihood of erroneous interpretations that could be easily drawn from nutrition studies when different agars have been used. As an alternative, we demonstrate the efficacy of a sterile and contamination-free hydroponic system for dissecting morphophysiological and molecular responses of Arabidopsis to different nutrient deficiencies.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Jain A,Poling MD,Smith AP,Nagarajan VK,Lahner B,Meagher RB,Raghothama KG

doi

10.1104/pp.109.136184

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

1033-49

issue

2

eissn

0032-0889

issn

1532-2548

pii

pp.109.136184

journal_volume

150

pub_type

杂志文章
  • Structure and properties of an engineered transketolase from maize.

    abstract::The gene specifying plastid transketolase (TK) of maize (Zea mays) was cloned from a cDNA library by southern blotting using a heterologous probe from sorghum (Sorghum bicolor). A recombinant fusion protein comprising thioredoxin of Escherichia coli and mature TK of maize was expressed at a high level in E. coli and c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.020982

    authors: Gerhardt S,Echt S,Busch M,Freigang J,Auerbach G,Bader G,Martin WF,Bacher A,Huber R,Fischer M

    更新日期:2003-08-01 00:00:00

  • Chloroplast Ultrastructure in Mutant Strains of Chlamydomonas reinhardi Lacking Components of the Photosynthetic Apparatus.

    abstract::The fine structure of the chloroplast of wild-type and 9 photosynthetic mutant strains of Chlamydomonas reinhardi is described. The chloroplast phenotypes of the mutant strains are clearly distinct from the wild type in all but 2 cases. Moreover, strains with similar photosynthetic disabilities have structurally simil...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.7.990

    authors: Goodenough UW,Levine RP

    更新日期:1969-07-01 00:00:00

  • The cytokinin type-B response regulator PtRR13 is a negative regulator of adventitious root development in Populus.

    abstract::Adventitious root formation at the base of plant cuttings is an innate de novo organogenesis process that allows massive vegetative propagation of many economically and ecologically important species. The early molecular events following shoot excision are not well understood. Using whole-genome microarrays, we detect...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.137505

    authors: Ramírez-Carvajal GA,Morse AM,Dervinis C,Davis JM

    更新日期:2009-06-01 00:00:00

  • Light and CO(2) Response of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activation in Arabidopsis Leaves.

    abstract::The requirements for activation of ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) were investigated in leaves of Arabidopsis wild-type and a mutant incapable of light activating rubisco in vivo. Upon illumination with saturating light intensities, the activation state of rubisco increased 2-fold in the wild...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.3.655

    authors: Salvucci ME,Portis AR,Ogren WL

    更新日期:1986-03-01 00:00:00

  • The role of the plant-specific ALTERED XYLOGLUCAN9 protein in Arabidopsis cell wall polysaccharide O-acetylation.

    abstract::A mutation in the ALTERED XYLOGLUCAN9 (AXY9) gene was found to be causative for the decreased xyloglucan acetylation phenotype of the axy9.1 mutant, which was identified in a forward genetic screen for Arabidopsis (Arabidopsis thaliana) mutants. The axy9.1 mutant also exhibits decreased O-acetylation of xylan, implyin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.256479

    authors: Schultink A,Naylor D,Dama M,Pauly M

    更新日期:2015-04-01 00:00:00

  • Maintenance of turgor by rapid sealing of puncture wounds in leaf epidermal cells.

    abstract::When leaf epidermal cells are puncture wounded with a glass microcapillary tip, a small droplet of fluid is discharged and then evaporates, leaving a solid residue on the cell surface. For puncture wounds of about 3.5 micrometers in diameter, this process is complete within 2 to 3 seconds. A second puncture wound also...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.3.907

    authors: Shackel KA,Polito VS,Ahmadi H

    更新日期:1991-11-01 00:00:00

  • Degree of C(4) Photosynthesis in C(4) and C(3)-C(4)Flaveria Species and Their Hybrids : II. Inhibition of Apparent Photosynthesis by a Phosphoenolpyruvate Carboxylase Inhibitor.

    abstract::Hybrids have been made between species of Flaveria exhibiting varying levels of C(4) photosynthesis. The degree of C(4) photosynthesis expressed in four interspecific hybrids (Flaveria trinervia [C(4)] x F. linearis [C(3)-C(4)], F. brownii [C(4)-like] x F. linearis, and two three-species hybrids from F. trinervia x [F...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.2.947

    authors: Brown RH,Byrd GT,Black CC

    更新日期:1992-10-01 00:00:00

  • The Arabidopsis family GT43 glycosyltransferases form two functionally nonredundant groups essential for the elongation of glucuronoxylan backbone.

    abstract::There exist four members of family GT43 glycosyltransferases in the Arabidopsis (Arabidopsis thaliana) genome, and mutations of two of them, IRX9 and IRX14, have previously been shown to cause a defect in glucuronoxylan (GX) biosynthesis. However, it is currently unknown whether IRX9 and IRX14 perform the same biochem...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.155309

    authors: Lee C,Teng Q,Huang W,Zhong R,Ye ZH

    更新日期:2010-06-01 00:00:00

  • Cytokinins of the Developing Mango Fruit : Isolation, Identification, and Changes in Levels during Maturation.

    abstract::The cytokinin activity has been isolated and identified from extracts of immature mango (Mangifera indica L.) seeds. The structures of zeatin, zeatin riboside, and N(6)-(Delta(2)-isopentenyl)adenine riboside were confirmed on the basis of their chromatographic behavior and mass spectra of trimethylsilyl derivatives. B...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.2.356

    authors: Chen WS

    更新日期:1983-02-01 00:00:00

  • Gains and Losses of Cis-regulatory Elements Led to Divergence of the Arabidopsis APETALA1 and CAULIFLOWER Duplicate Genes in the Time, Space, and Level of Expression and Regulation of One Paralog by the Other.

    abstract::How genes change their expression patterns over time is still poorly understood. Here, by conducting expression, functional, bioinformatic, and evolutionary analyses, we demonstrate that the differences between the Arabidopsis (Arabidopsis thaliana) APETALA1 (AP1) and CAULIFLOWER (CAL) duplicate genes in the time, spa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00320

    authors: Ye L,Wang B,Zhang W,Shan H,Kong H

    更新日期:2016-06-01 00:00:00

  • Abscisic Acid Content, Transpiration, and Stomatal Conductance As Related to Leaf Age in Plants of Xanthium strumarium L.

    abstract::Among the four uppermost leaves of greenhouse-grown plants of Xanthium strumarium L. the content of abscisic acid per unit fresh or dry weight was highest in the youngest leaf and decreased gradually with increasing age of the leaves. Expressed per leaf, the second youngest leaf was richest in ABA; the amount of ABA p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.2.169

    authors: Raschke K,Zeevaart JA

    更新日期:1976-08-01 00:00:00

  • Direct Inhibition of Plant Mitochondrial Respiration by Elevated CO2.

    abstract::Doubling the concentration of atmospheric CO2 often inhibits plant respiration, but the mechanistic basis of this effect is unknown. We investigated the direct effects of increasing the concentration of CO2 by 360 [mu]L L-1 above ambient on O2 uptake in isolated mitochondria from soybean (Glycine max L. cv Ransom) cot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.3.1349

    authors: Gonzalez-Meler MA,Ribas-Carbo M,Siedow JN,Drake BG

    更新日期:1996-11-01 00:00:00

  • Down-regulation of Kelch domain-containing F-box protein in Arabidopsis enhances the production of (poly)phenols and tolerance to ultraviolet radiation.

    abstract::Phenylpropanoid biosynthesis in plants engenders myriad phenolics with diverse biological functions. Phenylalanine ammonia-lyase (PAL) is the first committed enzyme in the pathway, directing primary metabolic flux into a phenylpropanoid branch. Previously, we demonstrated that the Arabidopsis (Arabidopsis thaliana) Ke...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.249136

    authors: Zhang X,Gou M,Guo C,Yang H,Liu CJ

    更新日期:2015-02-01 00:00:00

  • The Role of Plastids in the Expression of Nuclear Genes for Thylakoid Proteins Studied with Chimeric [beta]-Glucuronidase Gene Fusions.

    abstract::We have analyzed plastid and nuclear gene expression in tobacco seedlings using the carotenoid biosynthesis inhibitor nor-flurazon. mRNA levels for three nuclear-encoded chlorophyll-binding proteins of photosystem I and photosystem II (CAB I and II and the CP 24 apoprotein) are no longer detectable in photobleached se...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.4.1355

    authors: Bolle C,Sopory S,Lubberstedt T,Klosgen RB,Herrmann RG,Oelmuller R

    更新日期:1994-08-01 00:00:00

  • Immunological characterization of maize starch branching enzymes.

    abstract::Highly purified fractions of three starch branching enzymes from developing maize (Zea mays L.) endosperm were used to prepare antisera in rabbits. In double diffusion experiments, no immunoprecipitate was observed when branching enzyme IIa or IIb was tested against branching enzyme I antiserum. No immunoprecipitate w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.3.813

    authors: Fisher MB,Boyer CD

    更新日期:1983-07-01 00:00:00

  • Characterization of a xylose-specific antiserum that reacts with the complex asparagine-linked glycans of extracellular and vacuolar glycoproteins.

    abstract::Antibodies were raised against carrot (Daucus carota) cell wall beta-fructosidase that was either in a native configuration (this serum is called anti-betaF(1)) or chemically deglycosylated (anti-betaF(2)). The two antisera had completely different specificities when tested by immunoblotting. The anti-betaF(1) serum r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.3.1182

    authors: Laurière M,Laurière C,Chrispeels MJ,Johnson KD,Sturm A

    更新日期:1989-07-01 00:00:00

  • Some aspects of the control of root growth and georeaction: the involvement of indoleacetic Acid and abscisic Acid.

    abstract::Apical segments of roots of Zea mays L. cv. Orla and cv. Anjou show a strong georeaction during 7 hours geostimulation. This is abolished by detipping the segments and restored by replacing the tips upon the apical cut surfaces. After exodiffusion of endogenous indoleacetic acid (IAA) the retipped segments showed a si...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.5.1047

    authors: Pilet PE

    更新日期:1981-05-01 00:00:00

  • Water Stress Effects on Nitrogen Assimilation and Growth of Trifolium subterraneum L. Using Dinitrogen or Ammonium Nitrate.

    abstract::The relative effects of water stress on growth parameters of subterranean clover (Trifolium subterraneum L. cv. Woogenellup) dependent on either N(2) or 8 millimolar NH(4)NO(3) for N were examined. Whole-plant carbon exchange rate (CER), acetylene reduction (AR), dry matter production, and Kjeldahl N accumulation were...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.2.416

    authors: Dejong TM,Phillips DA

    更新日期:1982-02-01 00:00:00

  • Salicylic acid is an uncoupler and inhibitor of mitochondrial electron transport.

    abstract::The effect of salicylic acid (SA) on respiration and mitochondrial function was examined in tobacco (Nicotiana tabacum) suspension cell cultures in the range of 0.01 to 5 mm. Cells rapidly accumulated SA up to 10-fold of the externally applied concentrations. At the lower concentrations, SA accumulation was transitory...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.031039

    authors: Norman C,Howell KA,Millar AH,Whelan JM,Day DA

    更新日期:2004-01-01 00:00:00

  • Responses of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygen.

    abstract::Responses of superoxide dismutase (EC 1.15.1.1) and glutathione reductase (EC 1.6.4.2) activities were evaluated in leaf tissue from intact cotton plants (Cotton Branch 1697) which were exposed to 75% O(2), 350 microliters per liter CO(2) for 48 hours. Soluble protein was extracted from O(2)-treated and control tissue...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.3.482

    authors: Foster JG,Hess JL

    更新日期:1980-09-01 00:00:00

  • The Nitrogen Use Efficiency of C(3) and C(4) Plants: I. Leaf Nitrogen, Growth, and Biomass Partitioning in Chenopodium album (L.) and Amaranthus retroflexus (L.).

    abstract::The effect of applied nitrogen (N) on the growth, leaf expansion rate, biomass partitioning and leaf N levels of Chenopodium album (C(3)) and Amaranthus retroflexus (C(4)) were investigated. At a given applied N level, C. album had 50% greater leaf N per unit area (N(a)) than A. retroflexus. Nitrate accumulated at low...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.3.954

    authors: Sage RF,Pearcy RW

    更新日期:1987-07-01 00:00:00

  • Large Cellular Inclusions Accumulate in Arabidopsis Roots Exposed to Low-Sulfur Conditions.

    abstract::Sulfur is vital for primary and secondary metabolism in plant roots. To understand the molecular and morphogenetic changes associated with loss of this key macronutrient, we grew Arabidopsis (Arabidopsis thaliana) seedlings in low-sulfur conditions. These conditions induced a cascade of cellular events that converged ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00465

    authors: Jackson TL,Baker GW,Wilks FR Jr,Popov VA,Mathur J,Benfey PN

    更新日期:2015-08-01 00:00:00

  • Glyceollin Transcription Factor GmMYB29A2 Regulates Soybean Resistance to Phytophthora sojae.

    abstract::Glyceollin isomers I, II, and III are the major pathogen-elicited secondary metabolites (i.e. phytoalexins) of soybean (Glycine max) that, collectively with other 5-deoxyisoflavonoids, provide race-specific resistance to Phytophthora sojae. The NAC-family transcription factor (TF) GmNAC42-1 is an essential regulator o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01293

    authors: Jahan MA,Harris B,Lowery M,Infante AM,Percifield RJ,Kovinich N

    更新日期:2020-06-01 00:00:00

  • Voltage-sensor transitions of the inward-rectifying K+ channel KAT1 indicate a latching mechanism biased by hydration within the voltage sensor.

    abstract::The Kv-like (potassium voltage-dependent) K(+) channels at the plasma membrane, including the inward-rectifying KAT1 K(+) channel of Arabidopsis (Arabidopsis thaliana), are important targets for manipulating K(+) homeostasis in plants. Gating modification, especially, has been identified as a promising means by which ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.244319

    authors: Lefoulon C,Karnik R,Honsbein A,Gutla PV,Grefen C,Riedelsberger J,Poblete T,Dreyer I,Gonzalez W,Blatt MR

    更新日期:2014-10-01 00:00:00

  • Salicylic acid-dependent expression of host genes in compatible Arabidopsis-virus interactions.

    abstract::Plant viruses elicit the expression of common sets of genes in susceptible hosts. Studies in Arabidopsis (Arabidopsis thaliana) and tomato (Lycopersicon esculentum) indicate that at least one-third of the genes induced in common by viruses have been previously associated with plant defense and stress responses. The ge...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.056028

    authors: Huang Z,Yeakley JM,Garcia EW,Holdridge JD,Fan JB,Whitham SA

    更新日期:2005-03-01 00:00:00

  • Purification and Characterization of the Enzymes of Fructan Biosynthesis in Tubers of Helianthus tuberosus Colombia (II. Purification of Sucrose:Sucrose 1-Fructosyltransferase and Reconstitution of Fructan Synthesis in Vitro with Purified Sucrose:Sucrose

    abstract::Sucrose:sucrose 1-fructosyltransferase (1-SST), an enzyme involved in fructan biosynthesis, was purified to homogeneity from tubers of Helianthus tuberosus that were harvested in the accumulation phase. Gel filtration under native conditions predicted a molecular mass of about 67 kD. Electrophoresis or gel filtration ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.4.1167

    authors: Koops AJ,Jonker HH

    更新日期:1996-04-01 00:00:00

  • Proton Transport in Plasma Membrane and Tonoplast Vesicles from Red Beet (Beta vulgaris L.) Storage Tissue : A Comparative Study of Ion Effects on DeltapH and DeltaPsi.

    abstract::The proton transport properties of plasma membrane and tonoplast vesicles isolated from red beet (Beta vulgaris L.) storage tissue were examined and compared. Membrane vesicles isolated with 250 millimolar KCl in the homogenization media and recovered at low density following sucrose density gradient centrifugation di...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.3.613

    authors: Giannini JL,Briskin DP

    更新日期:1987-07-01 00:00:00

  • Phytochrome Pelletability Induced by Irradiation in Vivo: MIXING EXPERIMENTS.

    abstract::Samples of irradiated and control Avena sativa shoot tissue were homogenized together to determine whether, during homogenization phytochrome from irradiated tissue can bind to the particulate material simultaneously extracted from the control tissue. The level of phytochrome pelletability for such mixed tissue homoge...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.5.908

    authors: Quail PH,Briggs WR

    更新日期:1980-11-01 00:00:00

  • Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression.

    abstract::Aurora kinases are key effectors of mitosis. Plant Auroras are functionally divided into two clades. The alpha Auroras (Aurora1 and Aurora2) associate with the spindle and the cell plate and are implicated in controlling formative divisions throughout plant development. The beta Aurora (Aurora3) localizes to centromer...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01602

    authors: Boruc J,Weimer AK,Stoppin-Mellet V,Mylle E,Kosetsu K,Cedeño C,Jaquinod M,Njo M,De Milde L,Tompa P,Gonzalez N,Inzé D,Beeckman T,Vantard M,Van Damme D

    更新日期:2017-01-01 00:00:00

  • Ketose reductase activity in developing maize endosperm.

    abstract::Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.3.830

    authors: Doehlert DC

    更新日期:1987-07-01 00:00:00