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Characterization of a xylose-specific antiserum that reacts with the complex asparagine-linked glycans of extracellular and vacuolar glycoproteins.

Abstract：

:Antibodies were raised against carrot (Daucus carota) cell wall beta-fructosidase that was either in a native configuration (this serum is called anti-betaF(1)) or chemically deglycosylated (anti-betaF(2)). The two antisera had completely different specificities when tested by immunoblotting. The anti-betaF(1) serum reacted with beta-fructosidase and many other carrot cell wall proteins as well as with many proteins in extracts of bean (Phaseolus vulgaris) cotyledons and tobacco (Nicotiana tabacum) seeds. It did not react with chemically deglycosylated beta-fructosidase. The anti-betaF(1) serum also reacted with the bean vacuolar protein, phytohemagglutinin, but not with deglycosylated phytohemagglutinin. The anti-betaF(2) serum reacted with both normal and deglycosylated beta-fructosidase but not with other proteins. These results indicate that the betaF(2) antibodies recognize the beta-fructosidase polypeptide, while the betaF(1) antibodies recognize glycan sidechains common to many glycoproteins. We used immunoadsorption on glycoprotein-Sepharose columns and hapten inhibition of immunoblot reactions to characterize the nature of the antigenic site. Antibody binding activity was found to be associated with Man(3)(Xyl)(GIcNAc)(2)Fuc, Man(3)(Xyl)(GIcNAc)(2), and Man(Xyl) (GIcNAc)(2) glycans, but not with Man(3)(GIcNAc)(2). Treatment of phytohemagglutinin, a glycoprotein with a Man(3)(Xyl)(GIcNAc)(2)Fuc glycan, with Charonia lampas beta-xylosidase (after treatment with jack-bean alpha-mannosidase) greatly diminished the binding between the antibodies and phytohemagglutinin. We conclude, therefore, that the antibodies bind primarily to the xylosebeta, 1--> 2mannose structure commonly found in the complex glycans of plant glycoproteins.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Laurière M,Laurière C,Chrispeels MJ,Johnson KD,Sturm A

doi

10.1104/pp.90.3.1182

subject

Has Abstract

pub_date

1989-07-01 00:00:00

pages

1182-8

issue

eissn

0032-0889

issn

1532-2548

journal_volume

pub_type

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Characterization of a xylose-specific antiserum that reacts with the complex asparagine-linked glycans of extracellular and vacuolar glycoproteins.