Expression of Umbelopsis ramanniana DGAT2A in seed increases oil in soybean.

Abstract:

:Oilseeds are the main source of lipids used in both food and biofuels. The growing demand for vegetable oil has focused research toward increasing the amount of this valuable component in oilseed crops. Globally, soybean (Glycine max) is one of the most important oilseed crops grown, contributing about 30% of the vegetable oil used for food, feed, and industrial applications. Breeding efforts in soy have shown that multiple loci contribute to the final content of oil and protein stored in seeds. Genetically, the levels of these two storage products appear to be inversely correlated with an increase in oil coming at the expense of protein and vice versa. One way to overcome the linkage between oil and protein is to introduce a transgene that can specifically modulate one pathway without disrupting the other. We describe the first, to our knowledge, transgenic soy crop with increased oil that shows no major impact on protein content or yield. This was achieved by expressing a codon-optimized version of a diacylglycerol acyltransferase 2A from the soil fungus Umbelopsis (formerly Mortierella) ramanniana in soybean seed during development, resulting in an absolute increase in oil of 1.5% (by weight) in the mature seed.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Lardizabal K,Effertz R,Levering C,Mai J,Pedroso MC,Jury T,Aasen E,Gruys K,Bennett K

doi

10.1104/pp.108.123042

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

89-96

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.108.123042

journal_volume

148

pub_type

杂志文章
  • Abscission: the role of RNA synthesis.

    abstract::Ethylene stimulated the incorporation of (32)P into RNA in the abscission zone of bean explants (Phaseolus vulgaris L. var. Red Kidney). The enhancement was observed in all fractions separated by methylated albumin kieselguhr column chromatography, although the magnitude of the increase was not the same for each fract...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.8.1094

    authors: Holm RE,Abeles FB

    更新日期:1967-08-01 00:00:00

  • Gibberellin biosynthesis mutations and root development in pea.

    abstract::Dwarf mutants of pea (Pisum sativum), with impaired gibberellin (GA) biosynthesis in the shoot, were studied to determine whether the roots of these genotypes had altered elongation and GA levels. Mutations na, lh-2, and ls-1 reduced GA levels in root tips and taproot elongation, although in lh-2 and ls-1 roots the re...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.2.627

    authors: Yaxley JR,Ross JJ,Sherriff LJ,Reid JB

    更新日期:2001-02-01 00:00:00

  • Evidence That IAA Conjugates Are Slow-Release Sources of Free IAA in Plant Tissues.

    abstract::Evidence that indoleacetic acid (IAA) conjugates are metabolized via enzyme-catalyzed hydrolysis to free IAA and that their biological activities are related to the rates at which they are hydrolyzed by the tissue is presented. These conclusions are based on the following observations. Slow but continuous decarboxylat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.6.1424

    authors: Hangarter RP,Good NE

    更新日期:1981-12-01 00:00:00

  • Endogenous metabolism of fungus spores: stimulation by physical and chemical means.

    abstract::Endogenous respiration of spores of the fungus Myrothecium verrucaria can be stimulated up to over-10 fold by diverse chemicals or by physical treatments. Greatest effects were caused by azide (12-fold at 250 mum) and by 2,4-dinitrophenol (7-fold at 300 mum). Marked stimulation was also caused by 10 mum silver (5-fold...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.4.425

    authors: Mandels GR,Maguire A

    更新日期:1972-10-01 00:00:00

  • Engineering of a water-soluble plant cytochrome P450, CYP73A1, and NMR-based orientation of natural and alternate substrates in the active site.

    abstract::CYP73A1 catalyzes cinnamic acid hydroxylation, a reaction essential for the synthesis of lignin monomers and most phenolic compounds in higher plants. The native CYP73A1, initially isolated from Jerusalem artichoke (Helianthus tuberosus), was engineered to simplify purification from recombinant yeast and improve solub...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.020305

    authors: Schoch GA,Attias R,Belghazi M,Dansette PM,Werck-Reichhart D

    更新日期:2003-11-01 00:00:00

  • GIGANTEA enables drought escape response via abscisic acid-dependent activation of the florigens and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS.

    abstract::Modulation of the transition to flowering plays an important role in the adaptation to drought. The drought-escape (DE) response allows plants to adaptively shorten their life cycle to make seeds before severe stress leads to death. However, the molecular basis of the DE response is unknown. A screen of different Arab...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.217729

    authors: Riboni M,Galbiati M,Tonelli C,Conti L

    更新日期:2013-07-01 00:00:00

  • Is there a role for the apex in shoot geotropism?

    abstract::Experiments with horizontal etiolated sunflower (Helianthus annuus L.) seedlings supported centrally such that both apical and basal ends are free to react to geostimulus, revealed that the apical end commences curvature 1 to 2 hours earlier than the basal end. The later curvature in the basal region is a consequence ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.2.272

    authors: Hart JW,Macdonald IR

    更新日期:1984-02-01 00:00:00

  • Piperonylic acid, a selective, mechanism-based inactivator of the trans-cinnamate 4-hydroxylase: A new tool to control the flux of metabolites in the phenylpropanoid pathway

    abstract::Piperonylic acid (PA) is a natural molecule bearing a methylenedioxy function that closely mimics the structure of trans-cinnamic acid. The CYP73A subfamily of plant P450s catalyzes trans-cinnamic acid 4-hydroxylation, the second step of the general phenylpropanoid pathway. We show that when incubated in vitro with ye...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.118.1.209

    authors: Schalk M,Cabello-Hurtado F,Pierrel MA,Atanossova R,Saindrenan P,Werck-Reichhart D

    更新日期:1998-09-01 00:00:00

  • Bypassing Iron Storage in Endodermal Vacuoles Rescues the Iron Mobilization Defect in the natural resistance associated-macrophage protein3natural resistance associated-macrophage protein4 Double Mutant.

    abstract::To improve seed iron (Fe) content and bioavailability, it is crucial to decipher the mechanisms that control Fe storage during seed development. In Arabidopsis (Arabidopsis thaliana) seeds, most Fe is concentrated in insoluble precipitates, with phytate in the vacuoles of cells surrounding the vasculature of the embry...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00380

    authors: Mary V,Schnell Ramos M,Gillet C,Socha AL,Giraudat J,Agorio A,Merlot S,Clairet C,Kim SA,Punshon T,Guerinot ML,Thomine S

    更新日期:2015-09-01 00:00:00

  • Reduced Lignin Content and Altered Lignin Composition in Transgenic Tobacco Down-Regulated in Expression of L-Phenylalanine Ammonia-Lyase or Cinnamate 4-Hydroxylase.

    abstract::We analyzed lignin content and composition in transgenic tobacco (Nicotiana tabacum) lines altered in the expression of the early phenylpropanoid biosynthetic enzymes L-phenylalanine ammonia-lyase and cinnamate 4-hydroxylase (C4H). The reduction of C4H activity by antisense expression or sense suppression resulted in ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.1.41

    authors: Sewalt V,Ni W,Blount JW,Jung HG,Masoud SA,Howles PA,Lamb C,Dixon RA

    更新日期:1997-09-01 00:00:00

  • Differential Effects of a Substituted Pyridazinone, BASF 13-338, on Pathways of Monogalactosyldiacylglycerol Synthesis in Arabidopsis.

    abstract::We have examined the effects of the substituted pyridazinone herbicide, 4-chloro-5-(dimethylamino)-2-phenyl-3(2H)pyridazinone (BASF 13-338, Sandoz 9785), on the desaturation of linoleic acid (18:2) on different molecular species of monogalactosyldiacylglycerol (MGDG) and phosphatidylcholine (PC) in leaf tissue of Arab...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.3.684

    authors: Norman HA,John JB

    更新日期:1987-11-01 00:00:00

  • Allantoic Acid Synthesis in Soybean Root Nodule Cytosol via Xanthine Dehydrogenase.

    abstract::Allantoin and allantoic acid are the major forms of nitrogen transported from soybean nodules to other parts of the plant. Neither the pathway or the site of ureide synthesis has been demonstrated in root nodules.Bacteroid and cytosol (plant portion) fractions were prepared and the purity of each fraction was determin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.6.1203

    authors: Triplett EW,Blevins DG,Randall DD

    更新日期:1980-06-01 00:00:00

  • Interdependence of Nitrogen Nutrition and Photosynthesis in Pisum sativum L: I. Effect of Combined Nitrogen on Symbiotic Nitrogen Fixation and Photosynthesis.

    abstract::Photosynthesis, primary productivity, N content, and N(2) fixation were determined as a function of applied NH(4) (+) in peas (Pisum sativum L. cv. Alaska) which were inoculated or not inoculated with Rhizobium leguminosarum. Cabon dioxide exchange rate (CER) increased 10-fold, total N content 7-fold, and total dry we...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.1.127

    authors: Bethlenfalvay GJ,Abu-Shakra SS,Phillips DA

    更新日期:1978-07-01 00:00:00

  • Molecular characterization of the Abp1 5'-flanking region in maize and the teosintes.

    abstract::Auxin-binding protein 1 subsp. mays (ABP1) has been suggested as a receptor mediating auxin-induced cell expansion and differentiation. In maize (Zea mays), ABP1 is encoded by a single gene, Abp1. The TATA and CAAT promoter elements as well as the transcriptional start site were previously identified and all were foun...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.1.369

    authors: Elrouby N,Bureau TE

    更新日期:2000-09-01 00:00:00

  • Identification of microRNAs involved in pathogen-associated molecular pattern-triggered plant innate immunity.

    abstract::Pathogen-associated molecular patterns (PAMPs) trigger plant defenses when perceived by surface-localized immune receptors. PAMP-triggered immunity (PTI) plays a vital role in the resistance of plants to numerous potential pathogens. MicroRNA (miRNA) biogenesis is known to be important for PTI, but miRNA species invol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.151803

    authors: Li Y,Zhang Q,Zhang J,Wu L,Qi Y,Zhou JM

    更新日期:2010-04-01 00:00:00

  • Effect of water vapor pressure on the thermal decomposition of 2-chloroethylphosphonic Acid.

    abstract::Decomposition of 2-chloroethylphosphonic acid (Ethephon) was studied in dried films at various water vapor pressures (0.6 to 86.9 millimeters Hg, 3.2 to 93.9% relative humidity) and temperatures (20, 30, 40, and 50 C) at pH 6.3 and 7.0. The rate of decomposition could be determined equally well by [(14)C]Ethephon or e...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.3.474

    authors: Klein I,Lavee S,Ben-Tal Y

    更新日期:1979-03-01 00:00:00

  • The Golgi Localization of GnTI Requires a Polar Amino Acid Residue within Its Transmembrane Domain.

    abstract::The Golgi apparatus consists of stacked cisternae filled with enzymes that facilitate the sequential and highly controlled modification of glycans from proteins that transit through the organelle. Although the glycan processing pathways have been extensively studied, the underlying mechanisms that concentrate Golgi-re...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00310

    authors: Schoberer J,Liebminger E,Vavra U,Veit C,Grünwald-Gruber C,Altmann F,Botchway SW,Strasser R

    更新日期:2019-06-01 00:00:00

  • Isolation and Characterization of the Multiple 7S Globulins of Soybean Proteins.

    abstract::Two major proteins (the 7S and 11S globulins) of soybean (Glycine max) were simultaneously isolated by a simple method based on their different solubilities in dilute tris (hydroxymethyl) aminomethane buffers. The purified 7S globulins, which represented essentially the entire 7S soybean protein fraction capable of di...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.56.1.19

    authors: Thanh VH,Okubo K,Shibasaki K

    更新日期:1975-07-01 00:00:00

  • Genetic Interactions between PEROXIN12 and Other Peroxisome-Associated Ubiquitination Components.

    abstract::Most eukaryotic cells require peroxisomes, organelles housing fatty acid β-oxidation and other critical metabolic reactions. Peroxisomal matrix proteins carry peroxisome-targeting signals that are recognized by one of two receptors, PEX5 or PEX7, in the cytosol. After delivering the matrix proteins to the organelle, t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01211

    authors: Kao YT,Fleming WA,Ventura MJ,Bartel B

    更新日期:2016-11-01 00:00:00

  • Protein cryoprotective activity of a cytosolic small heat shock protein that accumulates constitutively in chestnut stems and is up-regulated by low and high temperatures.

    abstract::Heat shock, and other stresses that cause protein misfolding and aggregation, trigger the accumulation of heat shock proteins (HSPs) in virtually all organisms. Among the HSPs of higher plants, those belonging to the small HSP (sHSP) family remain the least characterized in functional terms. We analyzed the occurrence...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.035857

    authors: Lopez-Matas MA,Nuñez P,Soto A,Allona I,Casado R,Collada C,Guevara MA,Aragoncillo C,Gomez L

    更新日期:2004-04-01 00:00:00

  • The GH3 acyl adenylase family member PBS3 regulates salicylic acid-dependent defense responses in Arabidopsis.

    abstract::The pbs3-1 mutant, identified in a screen for Arabidopsis (Arabidopsis thaliana) mutants exhibiting enhanced susceptibility to the avirulent Pseudomonas syringae pathogen DC3000 (avrPphB), also exhibits enhanced susceptibility to virulent P. syringae strains, suggesting it may impact basal disease resistance. Because ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.097691

    authors: Nobuta K,Okrent RA,Stoutemyer M,Rodibaugh N,Kempema L,Wildermuth MC,Innes RW

    更新日期:2007-06-01 00:00:00

  • Fructokinase (Fraction IV) of Pea Seeds.

    abstract::A fructokinase (EC 2.7.1.4) was obtained from pea (Pisum sativum L.) seeds. This enzyme, termed fructokinase (fraction IV), was specific for fructose as substrate and had little activity with glucose or mannose. Excess fructose inhibited the enzyme at the optimum pH (8.2) but not at pH 6.6. MgATP was inhibitory at pH ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.5.666

    authors: Turner JF,Harrison DD,Copeland L

    更新日期:1977-11-01 00:00:00

  • A cyclic nucleotide-gated channel (CNGC16) in pollen is critical for stress tolerance in pollen reproductive development.

    abstract::Cyclic nucleotide-gated channels (CNGCs) have been implicated in diverse aspects of plant growth and development, including responses to biotic and abiotic stress, as well as pollen tube growth and fertility. Here, genetic evidence identifies CNGC16 in Arabidopsis (Arabidopsis thaliana) as critical for pollen fertilit...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.206888

    authors: Tunc-Ozdemir M,Tang C,Ishka MR,Brown E,Groves NR,Myers CT,Rato C,Poulsen LR,McDowell S,Miller G,Mittler R,Harper JF

    更新日期:2013-02-01 00:00:00

  • Reduced Photosystem II Activity and Accumulation of Viral Coat Protein in Chloroplasts of Leaves Infected with Tobacco Mosaic Virus.

    abstract::We previously reported (A Reinero, RN Beachy 1986 Plant Mol Biol 6:291-301) that coat protein (CP) of tobacco mosaic virus (TMV) accumulates in chloroplasts of systemically infected leaves. To determine the significance of such interaction we examined electron transport rates in chloroplasts containing different level...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.1.111

    authors: Reinero A,Beachy RN

    更新日期:1989-01-01 00:00:00

  • Functional and phylogenetic analyses of a conserved regulatory program in the phloem of minor veins.

    abstract::The minor-vein phloem of mature leaves is developmentally and physiologically distinct from the phloem in the rest of the vascular system. Phloem loading of transport sugars occurs in the minor veins, and consistent with this, galactinol synthase is expressed in the minor veins of melon (Cucumis melo) as part of the s...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.027714

    authors: Ayre BG,Blair JE,Turgeon R

    更新日期:2003-11-01 00:00:00

  • The isolation of spinach chloroplasts in pyrophosphate media.

    abstract::A simplified procedure (involving disruption in sorbitol-pyrophosphate mixtures) permits the separation of spinach chloroplasts which retain the ability to catalyze the photosynthetic assimilation of carbon dioxide and its associated oxygen evolution. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.9.1415

    authors: Cockburn W,Walker DA,Baldry CW

    更新日期:1968-09-01 00:00:00

  • Isolation of a Putative Carboxysomal Carbonic Anhydrase Gene from the Cyanobacterium Synechococcus PCC7942.

    abstract::The Type II mutants of the cyanobacterium Synechococcus PCC7942 (G.D. Price, M.R. Badger [1989] Plant Physiol 91: 514-525) are able to accumulate a large pool of inorganic carbon inside the cell, but are unable to utilize it for CO(2) fixation, resulting in a high CO(2)-requiring phenotype. We have isolated a 3.5-kb B...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.2.794

    authors: Yu JW,Price GD,Song L,Badger MR

    更新日期:1992-10-01 00:00:00

  • Arabidopsis seed development and germination is associated with temporally distinct metabolic switches.

    abstract::While the metabolic networks in developing seeds during the period of reserve accumulation have been extensively characterized, much less is known about those present during seed desiccation and subsequent germination. Here we utilized metabolite profiling, in conjunction with selective mRNA and physiological profilin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.086694

    authors: Fait A,Angelovici R,Less H,Ohad I,Urbanczyk-Wochniak E,Fernie AR,Galili G

    更新日期:2006-11-01 00:00:00

  • Effect of water stress on cell division and cell-division-cycle 2-like cell-cycle kinase activity in wheat leaves

    abstract::In wheat (Triticum aestivum) seedlings subjected to a mild water stress (water potential of -0.3 MPa), the leaf-elongation rate was reduced by one-half and the mitotic activity of mesophyll cells was reduced to 42% of well-watered controls within 1 d. There was also a reduction in the length of the zone of mesophyll c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.2.667

    authors: Schuppler U,He PH,John PC,Munns R

    更新日期:1998-06-01 00:00:00

  • Chlorophyll synthesis in chlorella I. Occurrence of a lag phase on initiation of a dilute culture.

    abstract::A culture of Chlorella established by 30-fold dilution of a culture already grown to a level of 15 ml packed cell volume per liter produces little chlorophyll for approximately 12 hours. Investigation of other characteristics such as nitrogen incorporation, increase in packed cell volume and dry weight as well as RNA ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.10.1695

    authors: Shugarman PM,Appleman D

    更新日期:1966-12-01 00:00:00