Abstract:
:Most eukaryotic cells require peroxisomes, organelles housing fatty acid β-oxidation and other critical metabolic reactions. Peroxisomal matrix proteins carry peroxisome-targeting signals that are recognized by one of two receptors, PEX5 or PEX7, in the cytosol. After delivering the matrix proteins to the organelle, these receptors are removed from the peroxisomal membrane or matrix. Receptor retrotranslocation not only facilitates further rounds of matrix protein import but also prevents deleterious PEX5 retention in the membrane. Three peroxisome-associated ubiquitin-protein ligases in the Really Interesting New Gene (RING) family, PEX2, PEX10, and PEX12, facilitate PEX5 retrotranslocation. However, the detailed mechanism of receptor retrotranslocation remains unclear in plants. We identified an Arabidopsis (Arabidopsis thaliana) pex12 Glu-to-Lys missense allele that conferred severe peroxisomal defects, including impaired β-oxidation, inefficient matrix protein import, and decreased growth. We compared this pex12-1 mutant to other peroxisome-associated ubiquitination-related mutants and found that RING peroxin mutants displayed elevated PEX5 and PEX7 levels, supporting the involvement of RING peroxins in receptor ubiquitination in Arabidopsis. Also, we observed that disruption of any Arabidopsis RING peroxin led to decreased PEX10 levels, as seen in yeast and mammals. Peroxisomal defects were exacerbated in RING peroxin double mutants, suggesting distinct roles of individual RING peroxins. Finally, reducing function of the peroxisome-associated ubiquitin-conjugating enzyme PEX4 restored PEX10 levels and partially ameliorated the other molecular and physiological defects of the pex12-1 mutant. Future biochemical analyses will be needed to determine whether destabilization of the RING peroxin complex observed in pex12-1 stems from PEX4-dependent ubiquitination on the pex12-1 ectopic Lys residue.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Kao YT,Fleming WA,Ventura MJ,Bartel Bdoi
10.1104/pp.16.01211subject
Has Abstractpub_date
2016-11-01 00:00:00pages
1643-1656issue
3eissn
0032-0889issn
1532-2548pii
pp.16.01211journal_volume
172pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The unicellular green alga Chlamydomonas reinhardtii is a widely used model organism for studies of oxygenic photosynthesis in eukaryotes. Here we describe the development of a resource for functional genomics of photosynthesis using insertional mutagenesis of the Chlamydomonas nuclear genome. Chlamydomonas cells were...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.055244
更新日期:2005-02-01 00:00:00
abstract::In this study, the pathway of β-citraurin biosynthesis, carotenoid contents and the expression of genes related to carotenoid metabolism were investigated in two varieties of Satsuma mandarin (Citrus unshiu), Yamashitabeni-wase, which accumulates β-citraurin predominantly, and Miyagawa-wase, which does not accumulate ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.223297
更新日期:2013-10-01 00:00:00
abstract::Exudate was collected periodically from the root systems of detopped tobacco plants. Volume, cations, and (42)K or (86)Rb transfer were measured. According to measurements of K by (42)K and by the flame photometer, when concentrations of KCl and KNO(3) were lower than 10(-2)m, the K in the exudate came mostly from a p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.2.238
更新日期:1967-02-01 00:00:00
abstract::alpha-Amylase levels in intact seeds of barley (Hordeum vulgare L. cv. Himalaya) reach a maximum at 3 to 4 days of germination while gibberellin levels continue to increase beyond 6 days of germination. In contrast to its effect on half seeds, gibberellic acid does not increase the total amount of alpha-amylase produc...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.48.2.137
更新日期:1971-08-01 00:00:00
abstract::Seedlings of maize (Zea mays L. cv Pioneer 3906), hydroponically grown in the dark, were exposed to NaCl either gradually (salt acclimation) or in one step (salt shock). In the salt-acclimation treatment, root extension was indistinguishable from that of unsalinized controls for at least 6 d at concentrations up to 10...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.3.881
更新日期:1997-03-01 00:00:00
abstract::Six Arabidopsis (Arabidopsis thaliana) clade A protein phosphatase 2Cs (PP2Cs) have established abscisic acid (ABA) signaling roles; however, phenotypic roles of the remaining three "HAI" PP2Cs, Highly ABA-Induced1 (HAI1), AKT1-Interacting PP2C1/HAI2, and HAI3, have remained unclear. HAI PP2C mutants had enhanced prol...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.202408
更新日期:2012-09-01 00:00:00
abstract::Flowering depends upon long-distance transport to supply water for reproductive mechanisms to function. Previous physiological studies suggested that flowers operated uncoupled from stem xylem transport and received water primarily from the phloem. We demonstrate that the water balance of Southern magnolia (Magnolia g...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.136127
更新日期:2009-07-01 00:00:00
abstract::Brassinosteroids (BRs) are a class of steroid hormones regulating multiple aspects of plant growth, development, and adaptation. Compared with extensive studies in Arabidopsis (Arabidopsis thaliana), the mechanism of BR signaling in rice (Oryza sativa) is less understood. Here, we identified OsWRKY53, a transcription ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00946
更新日期:2017-11-01 00:00:00
abstract::The presence of indole-3-butyric acid (IBA) as an endogenous auxin in Arabidopsis has been recently demonstrated. However, the in vivo role of IBA remains to be elucidated. We present the characterization of a semi-dominant mutant that is affected in its response to IBA, but shows a wild-type response to indole-3-acet...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.124.4.1739
更新日期:2000-12-01 00:00:00
abstract::The secretion of alpha-amylase from single isolated (Hordeum vulgare L. cv Himalaya) aleurone layers was studied in an automated flow-through apparatus. The apparatus, consisting of a modified sample analyzer linked to a chart recorder, automatically samples the flow-through medium at 1 minute intervals and assays for...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.4.1149
更新日期:1982-10-01 00:00:00
abstract::While the metabolic networks in developing seeds during the period of reserve accumulation have been extensively characterized, much less is known about those present during seed desiccation and subsequent germination. Here we utilized metabolite profiling, in conjunction with selective mRNA and physiological profilin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.086694
更新日期:2006-11-01 00:00:00
abstract::An elicitor prepared from the autoclaved cell walls of Phytophthora sp. induced O2- generation and H2O2 accumulation by cultured cells of Rosa damascena Mill. cv Gloire de Guilan. N,N-Diethyldithiocarbamate, a superoxide dismutase inhibitior, blocked H2O2 accumulation and caused a dramatic accumulation of O2- by elici...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.4.1241
更新日期:1995-04-01 00:00:00
abstract::Cauliflower mosaic virus (CaMV) replicated in protoplasts and in inoculated leaves of the non-host, cotton (Gossypium hirsutum, L.). Protoplasts prepared from suspension-cultured cotton cells were infected by incubation with liposome-encapsulated CaMV virions. During a 1-week culture period the amount of CaMV nucleic ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.3.633
更新日期:1987-03-01 00:00:00
abstract::Fructose-2,6-bisphosphate (Fru-2,6-bisP) was evaluated as a potential marker for the dormancy-breaking phase or the germination phase before pericarp splitting in red rice (Oryza sativa). During 4 h of imbibition at 30[deg]C, Fru-2,6-bisP of dehulled dormant and nondormant seeds increased to 0.26 and 0.38 pmol embryo-...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.4.1365
更新日期:1995-04-01 00:00:00
abstract::Reactive oxygen species (ROS) act as signal molecules for a variety of processes in plants. However, many questions about the roles of ROS in plants remain to be clarified. Here, we report the role of ROS in gibberellin (GA) and abscisic acid (ABA) signaling in barley (Hordeum vulgare) aleurone cells. The production o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.192740
更新日期:2012-04-01 00:00:00
abstract::In the last few years, much attention has been given to the role of proteins that accumulate during water deficit. In this work, we analyzed the electrophoretic patterns of basic protein extracts, enriched for a number of cell-wall proteins, from bean (Phaseolus vulgaris L.) seedlings and 21-d-old plants subjected to ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.4.1119
更新日期:1995-04-01 00:00:00
abstract::Photorespiration rates in tissue cultures of a C(4) plant, Portulaca oleracea, were compared to those in tissue cultures of a C(3) plant, Streptanthus tortuosus. The C(4) plant tissue cultures have one-half to one-third the photorespiration rate of the C(3) plant tissue cultures and respond to varying O(2) concentrati...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.4.573
更新日期:1976-10-01 00:00:00
abstract::Vesicular-arbuscular mycorrhizal fungi are symbionts for a large variety of crop plants; however, the form in which they take up carbon from the host is not established. To trace the course of carbon metabolism, we have used nuclear magnetic resonance spectroscopy with [13C]glucose labeling in vivo and in extracts to ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.1.7
更新日期:1995-05-01 00:00:00
abstract::The influence of plant spacing and row orientation on spectral distribution of light received by growing soybean (Gylcine max [L.] Merr.) plants was measured under field conditions. Light absorption, reflection and transmission of individual leaves showed that most of the blue and red was absorbed while most of the fa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.2.350
更新日期:1987-10-01 00:00:00
abstract::Synchronous cell division in Euglena gracilis (strain Z) was obtained in 24-hour light cycles consisting of 10 hours of light and 14 hours of darkness; cell division was restricted to the dark period. Photosynthetic capacity was found to vary in a cyclic manner during the cell cycle, reaching a peak 2 hours before the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.2.250
更新日期:1973-02-01 00:00:00
abstract::The evolution of C4 photosynthesis in many taxa involves the establishment of a two-celled photorespiratory CO2 pump, termed C2 photosynthesis. How C3 species evolved C2 metabolism is critical to understanding the initial phases of C4 plant evolution. To evaluate early events in C4 evolution, we compared leaf anatomy,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.221119
更新日期:2013-11-01 00:00:00
abstract::Soybean (Glycine max cv Hodgson) nitrogenase activity (C(2)H(2) reduction) in the presence or absence of nitrate was studied at various external O(2) tensions. Nitrogenase activity increased with oxygen partial pressure up to 30 kilopascals, which appeared to be the optimum. A parallel increase in ATP/ADP ratios indic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.1.224
更新日期:1989-05-01 00:00:00
abstract::A new bacterial agglutinin was isolated from seeds of the soybean cultivar Clark. Purification was carried out by ammonium sulfate precipitation and ion-exchange chromatography. The agglutinin is a heat-labile glycoprotein most active at pH 4.0. Addition of Ca(2+), Mn(2+) and Mg(2+) did not enhance the agglutinating a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.5.847
更新日期:1980-11-01 00:00:00
abstract::The growth regulator abscisic acid (ABA) can be used to induce dormant bud structures (turions) in the duckweed Spirodela polyrrhiza L. In this paper we show that during this process, ABA rapidly induces elevated levels of mRNA transcripts encoding a novel basic peroxidase. In addition, we show that in the presence of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.2.497
更新日期:1994-06-01 00:00:00
abstract::Cyanobacteria experience drastic changes in their carbon metabolism under daily light/dark cycles. During the day, the Calvin-Benson cycle fixes CO2 and diverts excess carbon into glycogen storage. At night, glycogen is degraded to support cellular respiration. The dark/light transition represents a universal environm...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.01184
更新日期:2020-01-01 00:00:00
abstract::Highly purified fractions of three starch branching enzymes from developing maize (Zea mays L.) endosperm were used to prepare antisera in rabbits. In double diffusion experiments, no immunoprecipitate was observed when branching enzyme IIa or IIb was tested against branching enzyme I antiserum. No immunoprecipitate w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.3.813
更新日期:1983-07-01 00:00:00
abstract::Carotenoid turnover was investigated in mature leaves of Arabidopsis (Arabidopsis thaliana) by 14CO2 pulse-chase labeling under control-light (CL; 130 micromol photons m(-2) s(-1)) and high-light (HL; 1,000 micromol photons m(-2) s(-1)) conditions. Following a 30-min 14CO2 administration, photosynthetically fixed 14C ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.151647
更新日期:2010-04-01 00:00:00
abstract::A new conjugate, 1-(gamma-L-glutamylamino)cyclopropane-1-carboxylic acid (GACC), of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) is identified. The only previously identified conjugate of ACC is 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC). GACC, not MACC, was the major conjugate formed ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.3.917
更新日期:1995-11-01 00:00:00
abstract::Several lines of evidence support the proposal that the unusual chloroplast-specific lipid acyl group Delta3,trans-hexadecenoic acid (trans-C(16:1)) stimulates the formation or maintenance of the oligomeric form of the light-harvesting chlorophyll a/b complex (LHCP). To assess the functional significance of this appar...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.4.853
更新日期:1985-08-01 00:00:00
abstract::Like many microalgae, Chlamydomonas reinhardtii forms lipid droplets rich in triacylglycerols when nutrient deprived. To begin studying the mechanisms underlying this process, nitrogen (N) deprivation was used to induce triacylglycerol accumulation and changes in developmental programs such as gametogenesis. Comparati...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.165159
更新日期:2010-12-01 00:00:00