Analysis of Photosynthetic Antenna Function in a Mutant of Arabidopsis thaliana (L.) Lacking trans-Hexadecenoic Acid.

Abstract:

:Several lines of evidence support the proposal that the unusual chloroplast-specific lipid acyl group Delta3,trans-hexadecenoic acid (trans-C(16:1)) stimulates the formation or maintenance of the oligomeric form of the light-harvesting chlorophyll a/b complex (LHCP). To assess the functional significance of this apparent association we have analyzed LHCP structure and function in a mutant of Arabidopsis thaliana (L.) which lacks trans-C(16:1) by electrophoretic analysis of the protein-chlorophyll complexes and by measurements of chlorophyll fluorescence under a variety of conditions. By these criteria the putative oligomeric form of LHCP appears to be slightly more labile to detergent-mediated dissociation in the mutant. The oligomeric PSI chlorophyll-protein complex, associated with PSI, was also more labile to detergent-mediated dissociation in the mutant, suggesting a previously unsuspected association of trans-C(16:1) with the PSI complex. However, no significant effect of the mutation on the efficiency of energy transfer from LHCP to the photochemical reaction centers was observed under any of the various conditions imposed. Also, the stability of the chlorophyll-protein complexes to temperature-induced dissociation was unaffected in the mutant. The role of trans-C(16:1) is very subtle or is only conditionally expressed.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

McCourt P,Browse J,Watson J,Arntzen CJ,Somerville CR

doi

10.1104/pp.78.4.853

subject

Has Abstract

pub_date

1985-08-01 00:00:00

pages

853-8

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

78

pub_type

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