A New Bacterial Agglutinin from Soybean: I. ISOLATION, PARTIAL PURIFICATION, AND CHARACTERIZATION.

abstract：:Zinc finger nucleases (ZFNs) are a powerful tool for genome editing in eukaryotic cells. ZFNs have been used for targeted mutagenesis in model and crop species. In animal and human cells, transient ZFN expression is often achieved by direct gene transfer into the target cells. Stable transformation, however, is the pr...

journal_title：Plant physiology

authors： Marton I,Zuker A,Shklarman E,Zeevi V,Tovkach A,Roffe S,Ovadis M,Tzfira T,Vainstein A

更新日期：2010-11-01 00:00:00

abstract：:Phototransformation of phytochrome in lettuce seeds (Lactuca sativa L. var. Grand Rapids) was examined by testing germination responses of seeds irradiated at various temperatures. Temperature variations from 0 to 50 C had no influence on the germination of partially hydrated seeds (about 15% water content) irradiated...

journal_title：Plant physiology

authors： Vidaver W,Hsiao AI

更新日期：1972-08-01 00:00:00

abstract：:Biosynthesis of sucrose from triacylglycerol requires the bypass of the CO2-evolving reactions of the tricarboxylic acid (TCA) cycle. The regulation of the TCA cycle bypass during lipid mobilization was examined. Lipid mobilization in Brassica napus was initiated shortly after imbibition of the seed and proceeded unti...

journal_title：Plant physiology

authors： Falk KL,Behal RH,Xiang C,Oliver DJ

更新日期：1998-06-01 00:00:00

abstract：:Dehydration inhibits petal expansion resulting in abnormal flower opening and results in quality loss during the marketing of cut flowers. We constructed a suppression subtractive hybridization library from rose (Rosa hybrida) flowers containing 3,513 unique expressed sequence tags and analyzed their expression profil...

journal_title：Plant physiology

authors： Dai F,Zhang C,Jiang X,Kang M,Yin X,Lü P,Zhang X,Zheng Y,Gao J

更新日期：2012-12-01 00:00:00

abstract：:Transgenic Medicago truncatula plants were produced harboring chimeric gene constructs of the glutamine synthetase (GS) cDNA clones (MtGS1a or MtGS1b) fused in sense or antisense orientation to the nodule-specific leghemoglobin promoter Mtlb1. A series of transgenic plants were obtained showing a 2- to 4-fold alterati...

journal_title：Plant physiology

authors： Carvalho HG,Lopes-Cardoso IA,Lima LM,Melo PM,Cullimore JV

更新日期：2003-09-01 00:00:00

abstract：:Glucosinolates (GSLs) are secondary metabolites whose degradation products confer intrinsic flavors and aromas to Brassicaceae vegetables. Several structures of GSLs are known in the Brassicaceae, and the biosynthetic pathway and regulatory networks have been elucidated in Arabidopsis (Arabidopsis thaliana). GSLs are ...

journal_title：Plant physiology

authors： Kakizaki T,Kitashiba H,Zou Z,Li F,Fukino N,Ohara T,Nishio T,Ishida M

更新日期：2017-03-01 00:00:00

abstract：:Ion and oxygen uptake were studied on aging bean stem slices. Oxygen uptake was high immediately after slicing, decreased to a minimum at 100 minutes, and then increased again. Ion uptake per unit of O(2) uptake data suggested that metabolic energy was utilized almost exclusively for sodium transport in fresh tissue b...

journal_title：Plant physiology

authors： Floyd RA,Rains DW

更新日期：1971-05-01 00:00:00

abstract：:Insertional mutagenesis using transfer DNA or transposable elements, which is an important tool in functional genomics and is well established in several crops, has not been developed in potato (Solanum tuberosum). Here, we report the application of the tobacco (Nicotiana tabacum) Tnt1 retrotransposon as an insertiona...

journal_title：Plant physiology

authors： Duangpan S,Zhang W,Wu Y,Jansky SH,Jiang J

更新日期：2013-09-01 00:00:00

abstract：:Branched-chain 2-oxo acids which are formed by transamination of leucine, isoleucine, and valine are metabolized by the peroxisomes from mung bean (Vigna radiata L.) hypocotyls. Acylcoenzyme A (CoA) thio ester intermediates of the pathways were separated by reversed-phase high performance liquid chromatography. Retent...

journal_title：Plant physiology

authors： Gerbling H,Gerhardt B

更新日期：1989-12-01 00:00:00

abstract：:While the importance of cell type specificity in plant adaptive responses is widely accepted, only a limited number of studies have addressed this issue at the functional level. We have combined electrophysiological, imaging, and biochemical techniques to reveal the physiological mechanisms conferring higher sensitivi...

journal_title：Plant physiology

authors： Shabala L,Zhang J,Pottosin I,Bose J,Zhu M,Fuglsang AT,Velarde-Buendia A,Massart A,Hill CB,Roessner U,Bacic A,Wu H,Azzarello E,Pandolfi C,Zhou M,Poschenrieder C,Mancuso S,Shabala S

更新日期：2016-12-01 00:00:00

abstract：:Carbonyl sulfide (COS), a substrate for carbonic anhydrase, inhibited alkalization of the medium, O(2) evolution, dissolved inorganic carbon accumulation, and photosynthetic CO(2) fixation at pH 7 or higher by five species of unicellular green algae that had been air-adapted for forming a CO(2)-concentrating process. ...

journal_title：Plant physiology

authors： Goyal A,Shiraiwa Y,Tolbert NE

更新日期：1992-02-01 00:00:00

abstract：:We have examined the expression of acyl carrier protein (ACP) mRNA levels and ACP activity in leaves, where fatty acids function primarily in membrane synthesis, and in developing soybean seeds, where fatty acids are primarily used for oil storage. An RNA probe transcribed from a synthetic spinach ACP-I gene hybridize...

journal_title：Plant physiology

authors： Hannapel DJ,Ohlrogge JB

更新日期：1988-04-01 00:00:00

abstract：:Signals can be perceived and amplified at the cell membrane by receptors coupled to the production of a variety of second messengers, including myoinositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)]. The myoinositol polyphosphate 5-phosphatases (5PTases; EC 3.1.3.56) comprise a large protein family that hydrolyzes 5-phospha...

journal_title：Plant physiology

authors： Gunesekera B,Torabinejad J,Robinson J,Gillaspy GE

更新日期：2007-03-01 00:00:00

abstract：:Measurements were made of (45)Ca influx into isolated internodal cells of Chara corallina and also into internodal cells of intact plants. (45)Ca influx was closely related to growth. In rapidly expanding internodal cells, the influx was approximately 1.4 nmol m(-2) s(-1) compared to the influx in mature cells from sl...

journal_title：Plant physiology

authors： Reid RJ,Smith FA

更新日期：1992-10-01 00:00:00

abstract：:Flowering of the facultative long-day plant Arabidopsis is controlled by several endogenous and environmental factors, among them gibberellins (GAs) and day length. The promotion of flowering by long days involves an endogenous clock that interacts with light cues provided by the environment. Light, and specifically p...

journal_title：Plant physiology

authors： Blázquez MA,Trénor M,Weigel D

更新日期：2002-12-01 00:00:00

abstract：:Intact peroxisomes were prepared from green leaves of a number of C(3) species, both monocots and dicots. A protoplast extract from which chloroplasts have been removed by a 1-minute 10,000g centrifugation was applied to a step gradient of 5, 15, 30, and 45% Percoll containing 0.5 molar sucrose, 0.1% BSA, and 25 milli...

journal_title：Plant physiology

authors： Schmitt MR,Edwards GE

更新日期：1982-10-01 00:00:00

abstract：:The JASMONATE ZIM DOMAIN (JAZ) proteins function as negative regulators of jasmonic acid signaling in plants. We cloned 12 JAZ genes from native tobacco (Nicotiana attenuata), including nine novel JAZs in tobacco, and examined their expression in plants that had leaves elicited by wounding or simulated herbivory. Most...

journal_title：Plant physiology

authors： Oh Y,Baldwin IT,Gális I

更新日期：2012-06-01 00:00:00

abstract：:The primary storage protein of oat (Avena sativa L.) seeds, globulin, was shown to have a specific carbohydrate-binding activity. The globulin was capable of hemagglutinating rabbit red blood cells and this hemagglutination was inhibited by the beta-glucan, laminarin, as well as by carbohydrate which had been cleaved ...

journal_title：Plant physiology

authors： Langston-Unkefer PJ,Gade W

更新日期：1984-03-01 00:00:00

abstract：:Waxy wheat (Triticum aestivum L.) lacks the waxy protein, which is also known as granule-bound starch synthase I (GBSSI). The starch granules of waxy wheat endosperm and pollen do not contain amylose and therefore stain red-brown with iodine. However, we observed that starch from pericarp tissue of waxy wheat stained ...

journal_title：Plant physiology

authors： Nakamura T,Vrinten P,Hayakawa K,Ikeda J

更新日期：1998-10-01 00:00:00

abstract：:Both salicylic acid and ferricyanide induce flowering in the long-day plant Lemna gibba L., strain G3 under 8- and 9-hour short days. In both cases the effect is daylength-dependent. Salicylic acid is ineffective on daylengths less than 8 hours and ferricyanide is ineffective on daylengths less than 5 hours. When both...

journal_title：Plant physiology

authors： Tanaka O,Cleland CF

更新日期：1980-06-01 00:00:00

abstract：:Plasmalemma redox activity in the diatom Thalassiosira is competitively inhibited by antiserum prepared against algal nitrate reductase (NR), and fluorescent labeling experiments reveal the binding of NR antiserum to the cell surface. Furthermore, the external electron acceptor Cu bathophenanthroline disulfonate cause...

journal_title：Plant physiology

authors： Jones GJ,Morel FM

更新日期：1988-05-01 00:00:00

abstract：:Superoxide dismutase was purified from pea (Pisum sativum L., cv. Wando) seeds and corn (Zea mays L., cv. Michigan 500) seedlings. The purified pea enzyme eluting as a single peak from gel exclusion chromatography columns contained the three electrophoretically distinct bands of superoxide dismutase characterizing the...

journal_title：Plant physiology

authors： Giannopolitis CN,Ries SK

更新日期：1977-02-01 00:00:00

abstract：:The DELLA protein REPRESSOR OF ga1-3-LIKE2 (RGL2) plays an important role in seed germination under different conditions through a number of transcription factors. However, the functions of the structural genes associated with RGL2-regulated germination are less defined. Here, we report the role of an Arabidopsis (Ara...

journal_title：Plant physiology

authors： Zhong C,Xu H,Ye S,Wang S,Li L,Zhang S,Wang X

更新日期：2015-11-01 00:00:00

abstract：:The mechanism of cytokinin-induced cell expansion in cotyledons excised from dark-grown seedlings of radish (Raphanus sativus L.) and cucumber (Cucumus sativus L.) was studied. Cotyledons were incubated in dim light with or without 17 micromolar zeatin for periods up to 3 days. Fresh weights and osmotic potentials wer...

journal_title：Plant physiology

authors： Thomas J,Ross CW,Chastain CJ,Koomanoff N,Hendrix JE

更新日期：1981-07-01 00:00:00

abstract：:The effect of ice encasement on the physiological, metabolic, and ultrastructural properties of winter wheat (Triticum aestivum L.) grown under field conditions was examined by artificially encasing winter wheat in ice during early winter. Cold hardiness and survival of ice-encased seedlings declined less rapidly in K...

journal_title：Plant physiology

authors： Pomeroy MK,Andrews CJ

更新日期：1978-05-01 00:00:00

abstract：:The anatomy of strawberry (Fragaria x ananassa) fruit, in which the achene is found on the outer part of the fruit, makes it an excellent species for studying the regulation of fruit development. It can provide a model for the cross talk between primary and secondary metabolism, whose role is of pivotal importance in ...

journal_title：Plant physiology

authors： Fait A,Hanhineva K,Beleggia R,Dai N,Rogachev I,Nikiforova VJ,Fernie AR,Aharoni A

更新日期：2008-10-01 00:00:00

abstract：:The effect of various periods of pretreatment in CaSO(4) solutions (aging) on the absorption of Na and K by bean stem slices was investigated. Freshly sliced tissue absorbed Na over the entire range of concentrations studied (0.02-50 mm). Potassium absorption by fresh tissue was nil at concentrations below 0.5 mm but ...

journal_title：Plant physiology

authors： Rains DW

更新日期：1969-04-01 00:00:00

abstract：:Auxin biosynthesis was analyzed in a maize (Zea mays) kernel culture system in which the seeds develop under physiological conditions similar to the in vivo situation. This system was modified for precursor feeding experiments. Tryptophan (Trp) is efficiently incorporated into indole-3-acetic acid (IAA) with retention...

journal_title：Plant physiology

authors： Glawischnig E,Tomas A,Eisenreich W,Spiteller P,Bacher A,Gierl A

更新日期：2000-07-01 00:00:00

abstract：:Ethylene production and respiration by Granny Smith apples were inhibited by treatment with 20% CO(2) for 2 hours. A similar effect was observed in tissue slices when treated at either 0 or 25 degrees C.The inhibition continued even after an extended aeration period. There is also an inhibition of ethylene emission in...

journal_title：Plant physiology

authors： Chaves AR,Tomás JO

更新日期：1984-09-01 00:00:00

abstract：:Ultraviolet-B (UV-B) photons can cause substantial cellular damage in biomolecules, as is well established for DNA. Because RNA has the same absorption spectrum for UV as DNA, we have investigated damage to this cellular constituent. In maize (Zea mays) leaves, UV-B radiation damages ribosomes by crosslinking cytosoli...

journal_title：Plant physiology

authors： Casati P,Walbot V

更新日期：2004-10-01 00:00:00