The Non-Mendelian Green Cotyledon Gene in Soybean Encodes a Small Subunit of Photosystem II.

Abstract:

:Chlorophyll degradation plays important roles in leaf senescence including regulation of degradation of chlorophyll-binding proteins. Although most genes encoding enzymes of the chlorophyll degradation pathway have been identified, the regulation of their activity has not been fully understood. Green cotyledon mutants in legume are stay-green mutants, in which chlorophyll degradation is impaired during leaf senescence and seed maturation. Among them, the soybean (Glycine max) green cotyledon gene cytG is unique because it is maternally inherited. To isolate cytG, we extensively sequenced the soybean chloroplast genome, and detected a 5-bp insertion causing a frame-shift in psbM, which encodes one of the small subunits of photosystem II. Mutant tobacco plants (Nicotiana tabacum) with a disrupted psbM generated using a chloroplast transformation technique had green senescent leaves, confirming that cytG encodes PsbM. The phenotype of cytG was very similar to that of mutant of chlorophyll b reductase catalyzing the first step of chlorophyll b degradation. In fact, chlorophyll b-degrading activity in dark-grown cytG and psbM-knockout seedlings was significantly lower than that of wild-type plants. Our results suggest that PsbM is a unique protein linking photosynthesis in presenescent leaves with chlorophyll degradation during leaf senescence and seed maturation. Additionally, we discuss the origin of cytG, which may have been selected during domestication of soybean.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Kohzuma K,Sato Y,Ito H,Okuzaki A,Watanabe M,Kobayashi H,Nakano M,Yamatani H,Masuda Y,Nagashima Y,Fukuoka H,Yamada T,Kanazawa A,Kitamura K,Tabei Y,Ikeuchi M,Sakamoto W,Tanaka A,Kusaba M

doi

10.1104/pp.16.01589

subject

Has Abstract

pub_date

2017-04-01 00:00:00

pages

2138-2147

issue

4

eissn

0032-0889

issn

1532-2548

pii

pp.16.01589

journal_volume

173

pub_type

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