Interactions of Avocado (Persea americana) Cytochrome P-450 with Monoterpenoids.

Abstract:

:The microsomal fraction of avocado (Persea americana) mesocarp is a rich source of cytochrome P-450 active in the demethylation of xenobiotics. Cytochrome P-450 from this tissue has been purified and well characterized at the molecular level (DP O'Keefe, KJ Leto [1989] Plant Physiol 89: 1141-1149; KR Bozak, H Yu, R Sirevag, RE Christoffersen [1990] Proc Natl Acad Sci USA 87: 3904-3908). Despite this extensive characterization, the role of the enzyme in vivo was not established. Optical and electron paramagnetic resonance binding studies described here suggest that the monoterpenoids, nerol and geraniol, are substrates of avocado cytochrome P-450 (spectral dissociation constant of 7.2 and 35 micromolar, respectively). Avocado microsomes have been shown to catalyze the hydroxylation of these monoterpenoids, and both nerol and geraniol have been shown to inhibit the activity of avocado cytochrome P-450 toward the artificial substrate 7-ethoxycoumarin, with nerol a competitive inhibitor of this activity.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Hallahan DL,Nugent JH,Hallahan BJ,Dawson GW,Smiley DW,West JM,Wallsgrove RM

doi

10.1104/pp.98.4.1290

subject

Has Abstract

pub_date

1992-04-01 00:00:00

pages

1290-7

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

98

pub_type

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