Abstract:
:ATP, which is present in the extracellular matrix of multicellular organisms and in the extracellular fluid of unicellular organisms, has been shown to function as a signaling molecule in animals. The concentration of extracellular ATP (xATP) is known to be functionally modulated in part by ectoapyrases, membrane-associated proteins that cleave the gamma- and beta-phosphates on xATP. We present data showing a previously unreported (to our knowledge) linkage between apyrase and phosphate transport. An apyrase from pea (Pisum sativum) complements a yeast (Saccharomyces cerevisiae) phosphate-transport mutant and significantly increases the amount of phosphate taken up by transgenic plants overexpressing the gene. The transgenic plants show enhanced growth and augmented phosphate transport when the additional phosphate is supplied as inorganic phosphate or as ATP. When scavenging phosphate from xATP, apyrase mobilizes the gamma-phosphate without promoting the transport of the purine or the ribose.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Thomas C,Sun Y,Naus K,Lloyd A,Roux Sdoi
10.1104/pp.119.2.543subject
Has Abstractpub_date
1999-02-01 00:00:00pages
543-52issue
2eissn
0032-0889issn
1532-2548journal_volume
119pub_type
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