Apyrase functions in plant phosphate nutrition and mobilizes phosphate from extracellular ATP.

Abstract:

:ATP, which is present in the extracellular matrix of multicellular organisms and in the extracellular fluid of unicellular organisms, has been shown to function as a signaling molecule in animals. The concentration of extracellular ATP (xATP) is known to be functionally modulated in part by ectoapyrases, membrane-associated proteins that cleave the gamma- and beta-phosphates on xATP. We present data showing a previously unreported (to our knowledge) linkage between apyrase and phosphate transport. An apyrase from pea (Pisum sativum) complements a yeast (Saccharomyces cerevisiae) phosphate-transport mutant and significantly increases the amount of phosphate taken up by transgenic plants overexpressing the gene. The transgenic plants show enhanced growth and augmented phosphate transport when the additional phosphate is supplied as inorganic phosphate or as ATP. When scavenging phosphate from xATP, apyrase mobilizes the gamma-phosphate without promoting the transport of the purine or the ribose.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Thomas C,Sun Y,Naus K,Lloyd A,Roux S

doi

10.1104/pp.119.2.543

subject

Has Abstract

pub_date

1999-02-01 00:00:00

pages

543-52

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

119

pub_type

杂志文章
  • Regulation of Nicotine Biosynthesis by an Endogenous Target Mimicry of MicroRNA in Tobacco.

    abstract::The interaction between noncoding endogenous target mimicry (eTM) and its corresponding microRNA (miRNA) is a newly discovered regulatory mechanism and plays pivotal roles in various biological processes in plants. Tobacco (Nicotiana tabacum) is a model plant for studying secondary metabolite alkaloids, of which nicot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00649

    authors: Li F,Wang W,Zhao N,Xiao B,Cao P,Wu X,Ye C,Shen E,Qiu J,Zhu QH,Xie J,Zhou X,Fan L

    更新日期:2015-10-01 00:00:00

  • Subcellular localization studies indicate that lipoxygenases 1 to 6 are not involved in lipid mobilization during soybean germination

    abstract::Soybean (Glycine max) lipoxygenase (LOX) has been proposed to be involved in reserve lipid mobilization during germination. Here, subcellular fractionation studies show that LOX1, -2, -3, -4, -5, and -6 isozymes were associated with the soluble fraction but not with purified oil bodies. The purified oil bodies contain...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.1.227

    authors: Wang C,Croft KP,Jarlfors U,Hildebrand DF

    更新日期:1999-05-01 00:00:00

  • Phytochrome action on chlorophyll synthesis-a study of the escape from photoreversibility.

    abstract::A brief red light pretreatment (pulse), operating through phytochrome, stimulates the synthesis of chlorophyll a and b in Sorghum vulgare shoots that are placed in continuous saturating white light. The red light effect is fully reversible by a far-red (756 nanometers) light pulse for 45 minutes. Thereafter, escape fr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.3.863

    authors: Oelze-Karow H,Mohr H

    更新日期:1982-09-01 00:00:00

  • Relative activities of NAD- and NADP- isocritric dehydrogenases in bean mitochondria modified by glycerol or NADP.

    abstract::Mitochondria from cotyledons of Vigna sesquipedalis (L.) Fruwirth (starchy seed) showed no NAD-isocitric dehydrogenase (NAD-IDH) activity by the methods which have been known to be useful for the detection of NAD-IDH in mitochondria of plants including castor bean and alaska pea. When the Vigna cotyledon mitochondria ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.2.262

    authors: Yamamoto Y

    更新日期:1969-02-01 00:00:00

  • Modification of Sulfhydryl Groups in the [gamma]-Subunit of Chloroplast-Coupling Factor 1 Affects the Proton Slip through the ATP Synthase.

    abstract::A large proton leak not coupled to ATP synthesis (slip) occurs at alkaline pH through the chloroplast ATP synthase (Y. Evron, M. Avron [1990] Biochim Biophys Acta 1019: 115-120). The involvement of the ATP synthase [gamma]-subunit in the regulation of proton conductance was analyzed by measuring the effect of thiolalk...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.4.1549

    authors: Evron Y,Pick U

    更新日期:1997-12-01 00:00:00

  • Chloroplast 2010: a database for large-scale phenotypic screening of Arabidopsis mutants.

    abstract::Large-scale phenotypic screening presents challenges and opportunities not encountered in typical forward or reverse genetics projects. We describe a modular database and laboratory information management system that was implemented in support of the Chloroplast 2010 Project, an Arabidopsis (Arabidopsis thaliana) reve...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.170118

    authors: Lu Y,Savage LJ,Larson MD,Wilkerson CG,Last RL

    更新日期:2011-04-01 00:00:00

  • Molecular Characterization of Arabidopsis GAL4/UAS Enhancer Trap Lines Identifies Novel Cell-Type-Specific Promoters.

    abstract::Cell-type-specific gene expression is essential to distinguish between the numerous cell types of multicellular organism. Therefore, cell-type-specific gene expression is tightly regulated and for most genes RNA transcription is the central point of control. Thus, transcriptional reporters are broadly used markers for...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00213

    authors: Radoeva T,Ten Hove CA,Saiga S,Weijers D

    更新日期:2016-06-01 00:00:00

  • Amino Acid Metabolism of Lemna minor L. : IV. N-Labeling Kinetics of the Amide and Amino Groups of Glutamine and Asparagine.

    abstract::A serious limitation to the use of N(O,S)-heptafluorobutyryl isobutyl amino acid derivatives in the analysis of (15)N-labeling kinetics of amino acids in plant tissues, is that the amides glutamine and asparagine undergo acid hydrolysis to glutamate and aspartate, respectively, during derivatization. This led us to co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.4.1161

    authors: Rhodes D,Rich PJ,Brunk DG

    更新日期:1989-04-01 00:00:00

  • L1 division and differentiation patterns influence shoot apical meristem maintenance.

    abstract::Plant development requires regulation of both cell division and differentiation. The class 1 KNOTTED1-like homeobox (KNOX) genes such as knotted1 (kn1) in maize (Zea mays) and SHOOTMERISTEMLESS in Arabidopsis (Arabidopsis thaliana) play a role in maintaining shoot apical meristem indeterminacy, and their misexpression...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.076075

    authors: Kessler S,Townsley B,Sinha N

    更新日期:2006-08-01 00:00:00

  • Messenger RNA-binding properties of nonpolysomal ribonucleoproteins from heat-stressed tomato cells

    abstract::Most cells experiencing heat stress reprogram their translational machinery to favor the synthesis of heat-stress proteins. Translation of other transcripts is almost completely repressed, but most untranslated messengers are not degraded. In contrast to yeast, Drosophila melanogaster, and HeLa cells, plant cells stor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.1.23

    authors: Stuger R,Ranostaj S,Materna T,Forreiter C

    更新日期:1999-05-01 00:00:00

  • Enzymes related to lactate metabolism in green algae and lower land plants.

    abstract::Cell-free extracts of Chlorella pyrenoidosa contained two enzymes capable of oxidizing d-lactate; these were glycolate dehydrogenase and NAD(+)-dependent d-lactate dehydrogenase. The two enzymes could be distinguished by differential centrifugation, glycolate dehydrogenase being largely particulate and NAD(+)-d-lactat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.2.167

    authors: Gruber PJ,Frederick SE,Tolbert NE

    更新日期:1974-02-01 00:00:00

  • Heat treatment results in a loss of transgene-encoded activities in several tobacco lines.

    abstract::Heat treatment (37 degrees C) of transgenic tobacco (Nicotiana tabacum) plants led to a reversible reduction or complete loss of transgene-encoded activities in about 40% of 10 independent transformants carrying the luciferase-coding region fused to the 355 cauliflower mosaic virus or the soybean small subunit promote...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.3.939

    authors: Neumann K,Dröge-Laser W,Köhne S,Broer I

    更新日期:1997-11-01 00:00:00

  • Phosphorus Distribution in Red Pine Roots and the Ectomycorrhizal Fungus Hebeloma arenosa.

    abstract::Red pines (Pinus resinosa Ait.) were grown in a pasteurized sandy loam either unamended with phosphate or fertilized with one of two levels of phosphate (34 or 136 mg/kg) as superphosphate, and with and without addition of Hebeloma arenosa inoculum. Shoot and total dry weights of mycorrhizal seedlings grown in soil un...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.2.713

    authors: Macfall JS,Slack SA,Wehrli S

    更新日期:1992-10-01 00:00:00

  • Flowering responses to altered expression of phytochrome in mutants and transgenic lines of Arabidopsis thaliana (L.) Heynh.

    abstract::The long-day plant Arabidopsis thaliana (L.) Heynh. flowers early in response to brief end-of-day (EOD) exposures to far-red light (FR) following a fluorescent short day of 8 h. FR promotion of flowering was nullified by subsequent brief red light (R) EOD exposure, indicating phytochrome involvement. The EOD response ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.4.1495

    authors: Bagnall DJ,King RW,Whitelam GC,Boylan MT,Wagner D,Quail PH

    更新日期:1995-08-01 00:00:00

  • Immunological Comparisons of Chymotrypsin Inhibitor I among Several Genera of the Solanaceae.

    abstract::Microcomplement fixation was employed to compare the immunological differences that occur between purified inhibitor I from potato tubers, the four purified protomers that comprise it, and inhibitor I from tuber and leaf extracts. Total inhibitors of chymotrypsin and trypsin in leaves of seven genera of the Solanaceae...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.5.627

    authors: Gurusiddaiah S,Kuo T,Ryan CA

    更新日期:1972-11-01 00:00:00

  • Optimization of Light-Harvesting Pigment Improves Photosynthetic Efficiency.

    abstract::Maximizing light capture by light-harvesting pigment optimization represents an attractive but challenging strategy to improve photosynthetic efficiency. Here, we report that loss of a previously uncharacterized gene, HIGH PHOTOSYNTHETIC EFFICIENCY1 (HPE1), optimizes light-harvesting pigments, leading to improved phot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00698

    authors: Jin H,Li M,Duan S,Fu M,Dong X,Liu B,Feng D,Wang J,Wang HB

    更新日期:2016-11-01 00:00:00

  • Two-Dimensional Electrophoretic Studies of the Proteins and Polypeptides in Mature Pollen Grains and the Male Germ Unit of Plumbago zeylanica.

    abstract::Three fractions (male germ unit [MGU]-rich, cytoplasmic-particulate and water-soluble proteins) were isolated from pollen of Plumbago zeylanica L. Proteins were extracted using a phenol procedure and polypeptide patterns were compared on one- and two-dimensional polyacrylamide gels. The MGU-rich fraction contains the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.3.764

    authors: Geltz NR,Russell SD

    更新日期:1988-11-01 00:00:00

  • A low-CO2-inducible gene encoding an alanine: alpha-ketoglutarate aminotransferase in Chlamydomonas reinhardtii.

    abstract::At low-CO2 (air) conditions, the unicellular green alga Chlamydomonas reinhardtii acquires the ability to raise its internal inorganic carbon concentration. To study this adaptation to low CO2, cDNA clones induced under low-CO2 growth conditions were selected through differential screening. One full-length clone is 25...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.2.677

    authors: Chen ZY,Burow MD,Mason CB,Moroney JV

    更新日期:1996-10-01 00:00:00

  • The G-Box Transcriptional Regulatory Code in Arabidopsis.

    abstract::Plants have significantly more transcription factor (TF) families than animals and fungi, and plant TF families tend to contain more genes; these expansions are linked to adaptation to environmental stressors. Many TF family members bind to similar or identical sequence motifs, such as G-boxes (CACGTG), so it is diffi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01086

    authors: Ezer D,Shepherd SJK,Brestovitsky A,Dickinson P,Cortijo S,Charoensawan V,Box MS,Biswas S,Jaeger KE,Wigge PA

    更新日期:2017-10-01 00:00:00

  • Virus-based microRNA expression for gene functional analysis in plants.

    abstract::Traditional virus-induced gene silencing (VIGS) is a powerful virus-based short interfering RNA-mediated RNA silencing technique for plant functional genomics. Besides short interfering RNAs, microRNAs (miRNAs) have also been shown to regulate gene expression by RNA silencing in various organisms. However, plant virus...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.155796

    authors: Tang Y,Wang F,Zhao J,Xie K,Hong Y,Liu Y

    更新日期:2010-06-01 00:00:00

  • OXA2b is Crucial for Proper Membrane Insertion of COX2 during Biogenesis of Complex IV in Plant Mitochondria.

    abstract::The evolutionarily conserved YidC/Oxa1/Alb3 proteins are involved in the insertion of membrane proteins in all domains of life. In plant mitochondria, individual knockouts of OXA1a, OXA2a, and OXA2b are embryo-lethal. In contrast to other members of the protein family, OXA2a and OXA2b contain a tetratricopeptide repea...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01286

    authors: Kolli R,Soll J,Carrie C

    更新日期:2019-02-01 00:00:00

  • Cell-Wall Proteins Induced by Water Deficit in Bean (Phaseolus vulgaris L.) Seedlings.

    abstract::In the last few years, much attention has been given to the role of proteins that accumulate during water deficit. In this work, we analyzed the electrophoretic patterns of basic protein extracts, enriched for a number of cell-wall proteins, from bean (Phaseolus vulgaris L.) seedlings and 21-d-old plants subjected to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.4.1119

    authors: Covarrubias AA,Ayala JW,Reyes JL,Hernandez M,Garciarrubio A

    更新日期:1995-04-01 00:00:00

  • Peroxisomal copper, zinc superoxide dismutase. Characterization of the isoenzyme from watermelon cotyledons.

    abstract::The biochemical and immunochemical characterization of a superoxide dismutase (SOD, EC 1.15.1.1) from peroxisomal origin has been carried out. The enzyme is a Cu,Zn-containing SOD (CuZn-SOD) located in the matrix of peroxisomes from watermelon (Citrullus vulgaris Schrad.) cotyledons (L.M. Sandalio and L.A. del Río [19...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.3.1151

    authors: Bueno P,Varela J,Gimeénez-Gallego G,del Río LA

    更新日期:1995-07-01 00:00:00

  • Ovule abortion in Arabidopsis triggered by stress.

    abstract::Environmental stresses frequently decrease plant fertility. In Arabidopsis, the effect of salt stress on reproduction was examined using plants grown in hydroponic medium. Salt stress inhibited microsporogenesis and stamen filament elongation. Because plants grown in hydroponic media can be rapidly and transiently str...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.043091

    authors: Sun K,Hunt K,Hauser BA

    更新日期:2004-08-01 00:00:00

  • Characterization of adenosine diphosphate glucose pyrophosphorylases from developing maize seeds.

    abstract::Electrophoretic examination of 22-day-old, normal maize (Zea mays L.) endosperm extracts revealed two zones of adenosine diphosphate glucose pyrophosphorylase activity. The enzymes are identical in terms of Km for glucose 1-phosphate and the effect of 3-phosphoglyceric acid on apparent Km for glucose 1-phosphate. Both...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.2.297

    authors: Hannah LC,Nelson OE

    更新日期:1975-02-01 00:00:00

  • Characterization of the Proton-Translocating Cytochrome c Oxidase Activity in the Plasma Membrane of Intact Anacystis nidulans Spheroplasts.

    abstract::Intact spheroplasts of the cyanobacterium (blue-green alga) Anacystis nidulans oxidized various exogenous c-type cytochromes with concomitant outward proton translocation while exogenous ferricytochrome c was not reduced. The H(+)/e(-) stoichiometry was close to 1 with each of the cytochromes and did not depend on the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.4.968

    authors: Peschek GA

    更新日期:1984-08-01 00:00:00

  • Toward supportive data collection tools for plant metabolomics.

    abstract::Over recent years, a number of initiatives have proposed standard reporting guidelines for functional genomics experiments. Associated with these are data models that may be used as the basis of the design of software tools that store and transmit experiment data in standard formats. Central to the success of such dat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.058875

    authors: Jenkins H,Johnson H,Kular B,Wang T,Hardy N

    更新日期:2005-05-01 00:00:00

  • Studies of a novel cysteine sulfoxide lyase from Petiveria alliacea: the first heteromeric alliinase.

    abstract::A novel alliinase (EC 4.4.1.4) was detected and purified from the roots of the Amazonian medicinal plant Petiveria alliacea. The isolated enzyme is a heteropentameric glycoprotein composed of two alpha-subunits (68.1 kD each), one beta-subunit (56.0 kD), one gamma-subunit (24.8 kD), and one delta-subunit (13.9 kD). Th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.142430

    authors: Musah RA,He Q,Kubec R,Jadhav A

    更新日期:2009-11-01 00:00:00

  • Saturation Kinetics of the Velocity of Stomatal Closing in Response to CO(2).

    abstract::Stomatal closing movements in response to changes from CO(2)-free to CO(2)-containing air were recorded in leaf sections of Zea mays using air flow porometers. The response to CO(2) was fast; the shortest lag between the application of 300 microliters CO(2) per liter of air and the beginning of a stomatal response was...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.2.229

    authors: Raschke K

    更新日期:1972-02-01 00:00:00

  • Phototropism and photoinhibition of basipolar transport of auxin in oat coleoptiles.

    abstract::We have proposed that the lateral inequality of auxin associated with the phototropic response is a consequence of a light-induced impairment of basipolar transport of the hormone. If this is so, the dose-response curve for photoinhibition of auxin transport should resemble that for phototropism. Further, the waveleng...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.4.491

    authors: Shen-Miller J,Cooper P,Gordon SA

    更新日期:1969-04-01 00:00:00