Peroxisomal copper, zinc superoxide dismutase. Characterization of the isoenzyme from watermelon cotyledons.


:The biochemical and immunochemical characterization of a superoxide dismutase (SOD, EC from peroxisomal origin has been carried out. The enzyme is a Cu,Zn-containing SOD (CuZn-SOD) located in the matrix of peroxisomes from watermelon (Citrullus vulgaris Schrad.) cotyledons (L.M. Sandalio and L.A. del Río [1988] Plant Physiol 88: 1215-1218). The amino acid composition of the enzyme was determined. Analysis by reversed-phase high-performance liquid chromatography of the peroxisomal CuZn-SOD incubated with 6 M guanidine-HCl indicated that this enzyme contained a noncovalently bound chromophore group that was responsible for the absorbance peak of the native enzyme at 260 nm. The amino acid sequence of the peroxisomal CuZn-SOD was determined by Edman degradation. Comparison of its sequence with those reported for other plant SODs revealed homologies of about 70% with cytosolic CuZn-SODs and of 90% with chloroplastic CuZn-SODs. The peroxisomal SOD has a high thermal stability and resistance to inactivation by hydrogen peroxide. A polyclonal antibody was raised against peroxisomal CuZn-SOD, and by western blotting the antibody cross-reacted with plant CuZn-SODs but did not recognize either plant Mn-SOD or bacterial Fe-SOD. The antiSOD-immunoglobulin G showed a weak cross-reaction with bovine erythrocytes and liver CuZn-SODs, and also with cell-free extracts from trout liver. The possible function of this CuZn-SOD in the oxidative metabolism of peroxisomes is discussed.


Plant Physiol


Plant physiology


Bueno P,Varela J,Gimeénez-Gallego G,del Río LA




Has Abstract


1995-07-01 00:00:00












  • Chloroplast and mitochondrial proteases in Arabidopsis. A proposed nomenclature.

    abstract::The identity and scope of chloroplast and mitochondrial proteases in higher plants has only started to become apparent in recent years. Biochemical and molecular studies suggested the existence of Clp, FtsH, and DegP proteases in chloroplasts, and a Lon protease in mitochondria, although currently the full extent of t...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Adam Z,Adamska I,Nakabayashi K,Ostersetzer O,Haussuhl K,Manuell A,Zheng B,Vallon O,Rodermel SR,Shinozaki K,Clarke AK

    更新日期:2001-04-01 00:00:00

  • Light effects on development of an indeterminate plant.

    abstract::The peanut (Arachis hypogaea L.) plant is indeterminate in growth habit and day-neutral with respect to flower initiation. The Spanish-type cultivar used in this study begins flowering 3 to 4 weeks from planting under optimum environmental conditions. In this study, irradiance and photoperiod were used to alter the de...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Ketring DL

    更新日期:1979-10-01 00:00:00

  • Induction of reduced photorespiratory activity in submersed and amphibious aquatic macrophytes.

    abstract::Incubation under water in a 30 C/14-hour or 12 C/10-hour photoperiod caused the CO(2) compensation points of 10 aquatic macrophytes to decrease below 25 or increase above 50 microliters CO(2) per liter, respectively. Submerged and aerial leaves of two amphibious angiosperms (Myriophyllum brasiliense and Proserpinaca p...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Salvucci ME,Bowes G

    更新日期:1981-02-01 00:00:00

  • Effects of Salinity on Water Transport of Excised Maize (Zea mays L.) Roots.

    abstract::The root pressure probe was used to determine the effects of salinity on the hydraulic properties of primary roots of maize (Zea mays L. cv Halamish). Maize seedlings were grown in nutrient solutions modified by additions of NaCl and/or extra CaCl(2) so that the seedlings received one of four treatments: Control, plus...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Azaizeh H,Steudle E

    更新日期:1991-11-01 00:00:00

  • Comparative Transcriptomic Approaches Exploring Contamination Stress Tolerance in Salix sp. Reveal the Importance for a Metaorganismal de Novo Assembly Approach for Nonmodel Plants.

    abstract::Metatranscriptomic study of nonmodel organisms requires strategies that retain the highly resolved genetic information generated from model organisms while allowing for identification of the unexpected. A real-world biological application of phytoremediation, the field growth of 10 Salix cultivars on polluted soils, w...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Brereton NJ,Gonzalez E,Marleau J,Nissim WG,Labrecque M,Joly S,Pitre FE

    更新日期:2016-05-01 00:00:00

  • Cytokinins in tobacco and wheat chloroplasts. Occurrence and changes due to light/dark treatment.

    abstract::Although cytokinins (CKs) affect a number of processes connected with chloroplasts, it has never been rigorously proven that chloroplasts contain CKs. We isolated intact chloroplasts from tobacco (Nicotiana tabacum L. cv SR1) and wheat (Triticum aestivum L. cv Ritmo) leaves and determined their CKs by liquid chromatog...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Benková E,Witters E,Van Dongen W,Kolár J,Motyka V,Brzobohatý B,Van Onckelen HA,Machácková I

    更新日期:1999-09-01 00:00:00

  • Silencing of soybean seed storage proteins results in a rebalanced protein composition preserving seed protein content without major collateral changes in the metabolome and transcriptome.

    abstract::The ontogeny of seed structure and the accumulation of seed storage substances is the result of a determinant genetic program. Using RNA interference, the synthesis of soybean (Glycine max) glycinin and conglycinin storage proteins has been suppressed. The storage protein knockdown (SP-) seeds are overtly identical to...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Schmidt MA,Barbazuk WB,Sandford M,May G,Song Z,Zhou W,Nikolau BJ,Herman EM

    更新日期:2011-05-01 00:00:00

  • Effects of gibberellic Acid and gold light on germination, enzyme activities, and amino Acid pool size in a dwarf strain of watermelon.

    abstract::Gibberellic acid (GA(3)) promotes and continuous gold light inhibits germination of seeds of a dwarf strain (WB-2) of watermelon [Citrullus lanatus (Thunb.) Matsu. and Nakai]. Osmotic inhibition of germination with mannitol in light-grown seeds of WB-2 was only slightly reversed by GA(3) at the concentrations used, wh...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Evensen KB,Loy JB

    更新日期:1978-07-01 00:00:00

  • Initial Bud Outgrowth Occurs Independent of Auxin Flow from Out of Buds.

    abstract::Apical dominance is the process whereby the shoot tip inhibits the growth of axillary buds along the stem. It has been proposed that the shoot tip, which is the predominant source of the plant hormone auxin, prevents bud outgrowth by suppressing auxin canalization and export from axillary buds into the main stem. In t...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Chabikwa TG,Brewer PB,Beveridge CA

    更新日期:2019-01-01 00:00:00

  • TAP46 plays a positive role in the ABSCISIC ACID INSENSITIVE5-regulated gene expression in Arabidopsis.

    abstract::TAP46 is a protein phosphatase2A (PP2A)-associated protein that regulates PP2A activity in Arabidopsis (Arabidopsis thaliana). To study how PP2A is involved in abscisic acid (ABA) signaling in plants, we studied the function of TAP46 in ABA-regulated seed maturation and seedling development. Expression of TAP46 coinci...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hu R,Zhu Y,Shen G,Zhang H

    更新日期:2014-02-01 00:00:00

  • Effects of changing stomatal width in a red pine forest on soil water content, leaf water potential, bole diameter, and growth.

    abstract::Spraying a 16 meter tall stand of red pine (Pinus resinosa Ait.) with 10(-3)m phenylmercuric acetate in early June and again in mid-July resulted in the water use between June 1 and October 25 being reduced by almost 10%. It was demonstrated that this was caused by an increase in the leaf resistance with partial stoma...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Turner NC,Waggoner PE

    更新日期:1968-06-01 00:00:00

  • The Arabidopsis Paf1c complex component CDC73 participates in the modification of FLOWERING LOCUS C chromatin.

    abstract::FLOWERING LOCUS C (FLC) is a key repressor of flowering in Arabidopsis (Arabidopsis thaliana) and is regulated, both positively and negatively, by posttranslational histone modifications. For example, vernalization (the promotion of flowering by cold temperatures) epigenetically silences FLC expression through repress...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Yu X,Michaels SD

    更新日期:2010-07-01 00:00:00

  • Specificities of the Acyl-Acyl Carrier Protein (ACP) Thioesterase and Glycerol-3-Phosphate Acyltransferase for Octadecenoyl-ACP Isomers (Identification of a Petroselinoyl-ACP Thioesterase in Umbelliferae).

    abstract::This study was designed to address the question: How specific for double bond position and conformation are plant enzymes that act on oleoyl-acyl carrier protein (ACP)? Octadecenoyl-ACPs with cis double bonds at positions [delta]6, [delta]7, [delta]8, [delta]9, [delta]10, [delta]11, or [delta]12 and elaidyl (18:1[delt...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Dormann P,Frentzen M,Ohlrogge JB

    更新日期:1994-03-01 00:00:00

  • Identification of a 1-aminocyclopropane-1-carboxylic acid synthase gene linked to the female (F) locus that enhances female sex expression in cucumber.

    abstract::Sex determination in cucumber (Cucumis sativus L.) is controlled largely by three genes: F, m, and a. The F and m loci interact to produce monoecious (M_f_) or gynoecious (M_f_) sex phenotypes. Ethylene and factors that induce ethylene biosynthesis, such as 1-aminocyclopropane-1-carboxylate (ACC) and auxin, also enhan...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Trebitsh T,Staub JE,O'Neill SD

    更新日期:1997-03-01 00:00:00

  • Influence of chloroplast development on the activation of the diphenyl ether herbicide acifluorfen-methyl.

    abstract::The activity of acifluorfen-methyl (AFM); methyl 5-(2-chloro-4-[trifluoromethyl] phenoxy)-2-nitrobenzoate in excised cucumber cotyledons (Cucumis sativus L.) was examined. AFM induced membrane disruption, was significantly greater when etiolated cotyledons were illuminated 16 hours at 150 microeinsteins per square met...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Halling BP,Peters GR

    更新日期:1987-08-01 00:00:00

  • Metabolism of 5-methylthioribose to methionine.

    abstract::During ethylene biosynthesis, the H(3)CS- group of S-adenosylmethionine is released as 5'-methylthioadenosine, which is recycled to methionine via 5-methylthioribose (MTR). In mungbean hypocotyls and cell-free extracts of avocado, [(14)C]MTR was converted into labeled methionine via 2-keto-4-methylthiobutyric acid (KM...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Miyazaki JH,Yang SF

    更新日期:1987-06-01 00:00:00

  • Manipulating broad-spectrum disease resistance by suppressing pathogen-induced auxin accumulation in rice.

    abstract::Breeding crops with the quality of broad-spectrum disease resistance using genetic resources is one of the principal goals of crop improvement. However, the molecular mechanism of broad-spectrum resistance remains largely unknown. Here, we show that GH3-2, encoding an indole-3-acetic acid (IAA)-amido synthetase, media...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Fu J,Liu H,Li Y,Yu H,Li X,Xiao J,Wang S

    更新日期:2011-01-01 00:00:00

  • Arabidopsis VAC14 Is Critical for Pollen Development through Mediating Vacuolar Organization.

    abstract::Pollen viability depends on dynamic vacuolar changes during pollen development involving increases and decreases of vacuolar volume through water and osmolite accumulation and vacuolar fission. Mutations in FAB1A to FAB1D, the genes encoding phosphatidylinositol 3,5-bisphosphate [PI(3,5)P2]-converting kinases, are mal...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Zhang WT,Li E,Guo YK,Yu SX,Wan ZY,Ma T,Li S,Hirano T,Sato MH,Zhang Y

    更新日期:2018-08-01 00:00:00

  • Development of the Potential for Cyanogenesis in Maturing Black Cherry (Prunus serotina Ehrh.) Fruits.

    abstract::Biochemical changes related to cyanogenesis (hydrogen cyanide production) were monitored during maturation of black cherry (Prunus serotina Ehrh.) fruits. At weekly intervals from flowering until maturity, fruits (or selected parts thereof) were analyzed for (a) fresh and dry weights, (b) prunasin and amygdalin levels...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Swain E,Li CP,Poulton JE

    更新日期:1992-04-01 00:00:00

  • Variation in Binding and Virulence of Agrobacterium tumefaciens Chromosomal Virulence (chv) Mutant Bacteria on Different Plant Species.

    abstract::Chromosomal virulence (chv) mutants of Agrobacterium tumefaciens have been reported to be deficient in binding to cells of zinnia, tobacco, and bamboo. The mutants are nonpathogenic on stems of Kalanchoë, sunflower, tomato, Jerusalem artichoke, and tobacco, but they cause tumors on tubers of Solanum tuberosum. We used...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hawes MC,Pueppke SG

    更新日期:1989-09-01 00:00:00

  • DELLA1-Mediated Gibberellin Signaling Regulates Cytokinin-Dependent Symbiotic Nodulation.

    abstract::In legume plants, low-nitrogen soils promote symbiotic interactions with rhizobial bacteria, leading to the formation of nitrogen-fixing root nodules. Among critical signals regulating this developmental process are bacterial Nod Factors (NFs) and several plant hormones, including cytokinins (CKs) and gibberellins (GA...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Fonouni-Farde C,Kisiala A,Brault M,Emery RJN,Diet A,Frugier F

    更新日期:2017-12-01 00:00:00

  • Regulation of Glyoxysomal Enzymes during Germination of Cucumber: 2. Isolation and Immunological Detection of Isocitrate Lyase and Catalase.

    abstract::The glyoxysomal enzymes isocitrate lyase and catalase have been isolated from etiolated cucumber (Cucumis sativus) cotyledons. The enzymes co-purified through polyethyleneimine precipitation and (NH(4))(2)SO(4) precipitation, and were resolved by gel filtration on Sepharose 6B followed by chromatography on diethylamin...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Lamb JE,Riezman H,Becker WM

    更新日期:1978-11-01 00:00:00

  • Kinetic Variance of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Isolated from Diverse Taxonomic Sources : II. Analysis by Two Dual Label Methods.

    abstract::Two dual label methods were used to investigate kinetic variability of ribulose 1,5-bisphosphate (RuBP) carboxylase/oxygenase (EC In addition to using [1-(14)C,5-(3)H]RuBP (method 1), we describe here the detailed assay with (14)CO(2) and [5-(3)H]RuBP (method 2), which generates [(3)H,(14)C]3-phosphoglyceri...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Kent SS,Tomany MJ

    更新日期:1984-07-01 00:00:00

  • Effect of arabinogalactan proteins from the root caps of pea and Brassica napus on Aphanomyces euteiches zoospore chemotaxis and germination.

    abstract::Root tips of many plant species release a number of border, or border-like, cells that are thought to play a major role in the protection of root meristem. However, little is currently known on the structure and function of the cell wall components of such root cells. Here, we investigate the sugar composition of the ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Cannesan MA,Durand C,Burel C,Gangneux C,Lerouge P,Ishii T,Laval K,Follet-Gueye ML,Driouich A,Vicré-Gibouin M

    更新日期:2012-08-01 00:00:00

  • TOPP4 Regulates the Stability of PHYTOCHROME INTERACTING FACTOR5 during Photomorphogenesis in Arabidopsis.

    abstract::In plants, photoreceptors transfer light signals to phytochrome-interacting factors (PIFs), inducing the rapid phosphorylation and degradation of PIFs to promote photomorphogenesis. However, the phosphatase responsible for PIF dephosphorylation remains unknown. In this study, we identified a type 1 protein phosphatase...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Yue J,Qin Q,Meng S,Jing H,Gou X,Li J,Hou S

    更新日期:2016-03-01 00:00:00

  • Snakin-2, an antimicrobial peptide from potato whose gene is locally induced by wounding and responds to pathogen infection.

    abstract::The peptide snakin-2 (StSN2) has been isolated from potato (Solanum tuberosum cv Jaerla) tubers and found to be active (EC(50) = 1-20 microM) against fungal and bacterial plant pathogens. It causes a rapid aggregation of both Gram-positive and Gram-negative bacteria. The corresponding StSN2 cDNA encodes a signal seque...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Berrocal-Lobo M,Segura A,Moreno M,López G,García-Olmedo F,Molina A

    更新日期:2002-03-01 00:00:00

  • The biogenesis of ethylene in penicillium digitatum.

    abstract::The origin of the ethylene carbon skeleton in Penicillium digitatum appears to be intimately associated with the Krebs cycle acids, particularly the middle carbon atoms of dicarboxylic acids. Among the other compounds studied, certain carbon atoms of beta-alanine, propionic acid, and methionine can be incorporated int...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Jacobsen DW,Wang CH

    更新日期:1968-12-01 00:00:00

  • Isolation and characterization of glutathione S-transferase isozymes from sorghum.

    abstract::Two glutathione S-transferase (GST) isozymes, A1/A1 and B1/B2, were purified from etiolated, O-1,3-dioxolan-2-yl-methyl-2,2,2, -trifluoro-4'-chloroacetophenone-oxime-treated sorghum (Sorghum bicolor L. Moench) shoots. GST A1/A1, a constitutively expressed homodimer, had a subunit molecular mass of 26 kD and an isoelec...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Gronwald JW,Plaisance KL

    更新日期:1998-07-01 00:00:00

  • The ARF7 and ARF19 Transcription Factors Positively Regulate PHOSPHATE STARVATION RESPONSE1 in Arabidopsis Roots.

    abstract::PHOSPHATE STARVATION RESPONSE1 (PHR1) is a key regulatory component of the response to phosphate (Pi) starvation. However, the regulation of PHR1 in this response remains poorly understood. Here, we report that PHR1 is a target of the transcription factors AUXIN RESPONSE FACTOR7 (ARF7) and ARF19 and is positively regu...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Huang KL,Ma GJ,Zhang ML,Xiong H,Wu H,Zhao CZ,Liu CS,Jia HX,Chen L,Kjorven JO,Li XB,Ren F

    更新日期:2018-09-01 00:00:00

  • Variations in the composition of gelling agents affect morphophysiological and molecular responses to deficiencies of phosphate and other nutrients.

    abstract::Low inorganic phosphate (Pi) availability triggers an array of spatiotemporal adaptive responses in Arabidopsis (Arabidopsis thaliana). There are several reports on the effects of Pi deprivation on the root system that have been attributed to different growth conditions and/or inherent genetic variability. Here we sho...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Jain A,Poling MD,Smith AP,Nagarajan VK,Lahner B,Meagher RB,Raghothama KG

    更新日期:2009-06-01 00:00:00