Abstract:
:More volatile organic carbon is lost from plants as isoprene than any other molecule. This flux of carbon to the atmosphere affects atmospheric chemistry and can serve as a substrate for ozone production in polluted air. Isoprene synthesis may help leaves cope with heatflecks and active oxygen species. Isoprene synthase, an enzyme related to monoterpene synthases, converts dimethylallyl diphosphate derived from the methylerythritol 4-phosphate pathway to isoprene. We used dideuterated deoxyxylulose (DOX-d(2)) to study the regulation of the isoprene biosynthetic pathway. Exogenous DOX-d(2) displaced endogenous sources of carbon for isoprene synthesis without increasing the overall rate of isoprene synthesis. However, at higher concentrations, DOX-d(2) completely suppressed isoprene synthesis from endogenous sources and increased the overall rate of isoprene synthesis. We interpret these results to indicate strong feedback control of deoxyxylulose-5-phosphate synthase. We related the emission of labeled isoprene to the concentration of labeled dimethylallyl diphosphate in order to estimate the in situ K(m) of isoprene synthase. The results confirm that isoprene synthase has a K(m) 10- to 100-fold higher for its allylic diphosphate substrate than related monoterpene synthases for geranyl diphosphate.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Wolfertz M,Sharkey TD,Boland W,Kühnemann Fdoi
10.1104/pp.104.043737subject
Has Abstractpub_date
2004-08-01 00:00:00pages
1939-45issue
4eissn
0032-0889issn
1532-2548pii
pp.104.043737journal_volume
135pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Ribulose 1,5-bisphosphate carboxylase (RuBPCase) was chosen as a model protein to study how heat shock (HS) affects both chloroplast protein synthesis and the nuclear-chloroplast interaction in production of chloroplast proteins. Experiments were performed using highly chlorophyllous, soybean (Glycine max L. Merr. var...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.1.155
更新日期:1985-05-01 00:00:00
abstract::The isolation of chloroplast and nuclear DNA from dark- and light-grown, control- and 3-amino-1,2,4-triazole-treated wheat seedlings, Triticum vulgare, is described. Contrary to a previous report, we found that chloroplast and nuclear DNA had similar buoyant densities (1.702 grams per cubic centimeter) and that they c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.46.6.825
更新日期:1970-12-01 00:00:00
abstract::A nonphotosynthetic phosphoenolpyruvate carboxylase (EC 4.1.1.31) was partially purified from the cytosol of root nodules of alfalfa. The enzyme was purified 86-fold by ammonium sulfate fractionation, DEAE-cellulose, hydroxylapatite chromatography, and reactive agarose with a final yield of 32%. The enzyme exhibited a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.1.261
更新日期:1984-05-01 00:00:00
abstract::Heterotrimeric G proteins have been shown to transmit ultraviolet B (UV-B) signals in mammalian cells, but whether they also transmit UV-B signals in plant cells is not clear. In this paper, we report that 0.5 W m(-2) UV-B induces stomatal closure in Arabidopsis (Arabidopsis thaliana) by eliciting a cascade of intrace...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.211623
更新日期:2013-03-01 00:00:00
abstract::The isoenzyme pattern of chorismate mutase (EC 5.4.99.5) was examined by diethylaminoethyl-cellulose chromatography in a wide variety of plants. All plants contained a regulated form of chorismate mutase (CM-1), and most contained an additional, unregulated form (CM-2). The regulatory properties of CM-1 differed signi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.3.717
更新日期:1986-07-01 00:00:00
abstract::Protein and RNA synthesizing activities increased markedly during the mating process and decreased during the maturation stage of zygotes in heterothallic strains of Closterium peracerosum-strigosum-littoale, KAS-4-29 (mating-type minus) and KAS -4-30 (mating-type plus) and a homothallic Closterium acerosum. Different...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.4.696
更新日期:1985-08-01 00:00:00
abstract::The plant cuticle is an extracellular hydrophobic layer that covers the aerial epidermis of all land plants, providing protection against desiccation and external environmental stresses. The past decade has seen considerable progress in assembling models for the biosynthesis of its two major components, the polymer cu...
journal_title:Plant physiology
pub_type: 杂志文章,评审
doi:10.1104/pp.113.222737
更新日期:2013-09-01 00:00:00
abstract::The effects of water stress on nitrate reductase and nitrite reductase activities in symbiotic nodules were examined in field-grown soybean plants (Glycine max L Merr. cv Clark). The in vitro assays of enzyme activity indicated that the nodule cytosol and bacteroids contained both nitrate reductase and nitrite reducta...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.4.1065
更新日期:1983-12-01 00:00:00
abstract::Grain size is one of the key determinants of grain yield. Although a number of genes that control grain size in rice (Oryza sativa) have been identified, the overall regulatory networks behind this process remain poorly understood. Here, we report the map-based cloning and functional characterization of the quantitati...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.01574
更新日期:2019-08-01 00:00:00
abstract::In many plant species, a subset of the genes of the chloroplast genome is transcribed by RpoTp, a nuclear-encoded plastid-targeted RNA polymerase. Here, we describe the positional cloning of the SCABRA3 (SCA3) gene, which was found to encode RpoTp in Arabidopsis (Arabidopsis thaliana). We studied one weak (sca3-1) and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.080069
更新日期:2006-07-01 00:00:00
abstract::Endogenous abscisic acid (ABA) levels are regulated by both biosynthesis and catabolism of the hormone. ABA 8'-hydroxylase is considered to be the key catabolic enzyme in many physiological processes. We have previously identified that four members of the Arabidopsis (Arabidopsis thaliana) CYP707A gene family (CYP707A...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.079475
更新日期:2006-05-01 00:00:00
abstract::Calcium (Ca) and manganese (Mn) are essential nutrients required for normal plant growth and development, and transport processes play a key role in regulating their cellular levels. Arabidopsis (Arabidopsis thaliana) contains four P(2A)-type ATPase genes, AtECA1 to AtECA4, which are expressed in all major organs of A...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.110817
更新日期:2008-01-01 00:00:00
abstract::Wood of trees is formed from carbon assimilated in the photosynthetic tissues. Determining the temporal dynamics of carbon assimilation, subsequent transport into developing wood, and incorporation to cell walls would further our understanding of wood formation in particular and tree growth in general. To investigate ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00292
更新日期:2015-06-01 00:00:00
abstract::Protein phosphorylation, catalyzed by the opposing actions of protein kinases and phosphatases, is a cornerstone of cellular signaling and regulation. Since their discovery, protein phosphatases have emerged as highly regulated enzymes with specificity that rivals their counteracting kinase partners. However, despite ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.182493
更新日期:2011-12-01 00:00:00
abstract::Relative water content, solute concentrations, and osmolality were determined in the water storage tissue (hydrenchyma) and the assimilatory tissue (chlorenchyma) of the succulent leaves of Peperomia magnoliaefolia (Jac) (Piperaceae) during slow desiccation. Relative water loss was significantly greater for the hydren...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.1.190
更新日期:1987-01-01 00:00:00
abstract::The citrate carrier from maize (Zea mays) shoot mitochondria was solubilized with Triton X-100 and purified by sequential chromatography on hydroxyapatite and hydroxyapatite/celite in the presence of cardiolipin. SDS-gel electrophoresis of the purified fraction showed a single polypeptide band with an apparent molecul...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.120.3.841
更新日期:1999-07-01 00:00:00
abstract::The separate package and spillover models of photosynthetic enhancement are generalized and extended to light saturation assuming hyperbolic or exponential rate versus intensity curves. The available data are more consistent with the exponential model at high light intensities. Two significant conclusions follow: (a) ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.47.2.222
更新日期:1971-02-01 00:00:00
abstract::We previously cloned and sequenced a cDNA encoding soybean ferric leghemoglobin reductase (FLbR), an enzyme postulated to play an important role in maintaining leghemoglobin in a functional ferrous state in nitrogen-fixing root nodules. This cDNA was sub-cloned into an expression plasmid, pTrcHis C, and overexpressed ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.1.203
更新日期:1994-09-01 00:00:00
abstract::Rapidly dried Tortula ruralis, a drought-tolerant moss, is known to synthesize proteins on rehydration at a much lower rate than the slowly dried moss. The reasons for this low rate of protein synthesis are unclear. We have found that during rehydration of rapidly dried moss, there is a negative correlation between th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.4.1094
更新日期:1987-12-01 00:00:00
abstract::Red pines (Pinus resinosa Ait.) were grown in a pasteurized sandy loam either unamended with phosphate or fertilized with one of two levels of phosphate (34 or 136 mg/kg) as superphosphate, and with and without addition of Hebeloma arenosa inoculum. Shoot and total dry weights of mycorrhizal seedlings grown in soil un...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.2.713
更新日期:1992-10-01 00:00:00
abstract::Synchronous cell division in Euglena gracilis (strain Z) was obtained in 24-hour light cycles consisting of 10 hours of light and 14 hours of darkness; cell division was restricted to the dark period. Photosynthetic capacity was found to vary in a cyclic manner during the cell cycle, reaching a peak 2 hours before the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.2.250
更新日期:1973-02-01 00:00:00
abstract::Gliadins and glutenins, the major storage proteins of wheat endosperm (Triticum durum, Desf. cv Monroe), were reduced in vitro by the NADP/thioredoxin system (NADPH, NADP-thioredoxin reductase and thioredoxin; in plants, the h type) from either the same source or the bacterium Escherichia coli. A more limited reducti...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.99.3.919
更新日期:1992-07-01 00:00:00
abstract::Chlorogenic acids (CGAs) are a group of phenolic secondary metabolites produced by certain plant species and an important component of coffee (Coffea spp.). The CGAs have been implicated in biotic and abiotic stress responses, while the related shikimate esters are key intermediates for lignin biosynthesis. Here, two ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.202051
更新日期:2012-09-01 00:00:00
abstract::Here, we investigated the physiological role of Arabidopsis (Arabidopsis thaliana) AtNUDX6, the gene encoding ADP-ribose (Rib)/NADH pyrophosphohydrolase, using its overexpressor (Pro35S:AtNUDX6) or disruptant (KO-nudx6). The level of NADH in Pro35S:AtNUDX6 and KO-nudx6 plants was decreased and increased, respectively,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.153569
更新日期:2010-04-01 00:00:00
abstract::Fruit removal for 5 weeks after flowering increased acid phosphatase activity 10-fold in soybean (Glycine max L. Merr. Var Hobbit) leaves compared with normal seed-pod-bearing plants. The major acid phosphatase activity in leaves was purified over 2700-fold, yielding a single polypeptide of 51 kD with a specific activ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.1.49
更新日期:1994-01-01 00:00:00
abstract::The concept of system 1 and system 2 ethylene biosynthesis during climacteric fruit ripening was initially described four decades ago. Although much is known about fruit development and climacteric ripening, little information is available about how ethylene biosynthesis is regulated during the postclimacteric phase. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.206086
更新日期:2012-11-01 00:00:00
abstract::Oilseeds are the main source of lipids used in both food and biofuels. The growing demand for vegetable oil has focused research toward increasing the amount of this valuable component in oilseed crops. Globally, soybean (Glycine max) is one of the most important oilseed crops grown, contributing about 30% of the vege...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.123042
更新日期:2008-09-01 00:00:00
abstract::Receptor-like kinases (RLKs) are the largest family of proteins in plants and are responsible for perceiving the vast majority of extracellular stimuli. Thus, RLKs function in diverse processes, including sensing pathogen attacks, regulating symbiotic interactions, transducing hormone and peptide signals, and monitori...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01700
更新日期:2018-06-01 00:00:00
abstract::Plants respond to low carbon supply by massive reprogramming of the transcriptome and metabolome. We show here that the carbon starvation-induced NAC (for NO APICAL MERISTEM/ARABIDOPSIS TRANSCRIPTION ACTIVATION FACTOR/CUP-SHAPED COTYLEDON) transcription factor Arabidopsis (Arabidopsis thaliana) Transcription Activatio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00917
更新日期:2015-09-01 00:00:00
abstract::We previously reported (A Reinero, RN Beachy 1986 Plant Mol Biol 6:291-301) that coat protein (CP) of tobacco mosaic virus (TMV) accumulates in chloroplasts of systemically infected leaves. To determine the significance of such interaction we examined electron transport rates in chloroplasts containing different level...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.1.111
更新日期:1989-01-01 00:00:00