Rapid regulation of the methylerythritol 4-phosphate pathway during isoprene synthesis.


:More volatile organic carbon is lost from plants as isoprene than any other molecule. This flux of carbon to the atmosphere affects atmospheric chemistry and can serve as a substrate for ozone production in polluted air. Isoprene synthesis may help leaves cope with heatflecks and active oxygen species. Isoprene synthase, an enzyme related to monoterpene synthases, converts dimethylallyl diphosphate derived from the methylerythritol 4-phosphate pathway to isoprene. We used dideuterated deoxyxylulose (DOX-d(2)) to study the regulation of the isoprene biosynthetic pathway. Exogenous DOX-d(2) displaced endogenous sources of carbon for isoprene synthesis without increasing the overall rate of isoprene synthesis. However, at higher concentrations, DOX-d(2) completely suppressed isoprene synthesis from endogenous sources and increased the overall rate of isoprene synthesis. We interpret these results to indicate strong feedback control of deoxyxylulose-5-phosphate synthase. We related the emission of labeled isoprene to the concentration of labeled dimethylallyl diphosphate in order to estimate the in situ K(m) of isoprene synthase. The results confirm that isoprene synthase has a K(m) 10- to 100-fold higher for its allylic diphosphate substrate than related monoterpene synthases for geranyl diphosphate.


Plant Physiol


Plant physiology


Wolfertz M,Sharkey TD,Boland W,Kühnemann F




Has Abstract


2004-08-01 00:00:00














  • The hpa1 mutant of Arabidopsis reveals a crucial role of histidine homeostasis in root meristem maintenance.

    abstract::Histidine (His) is an essential ingredient for protein synthesis and is required by all living organisms. In higher plants, although there is considerable evidence that His is essential for plant growth and survival, there is very little information as to whether it plays any specific role in plant development. Here, ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Mo X,Zhu Q,Li X,Li J,Zeng Q,Rong H,Zhang H,Wu P

    更新日期:2006-08-01 00:00:00

  • Cold-regulated cereal chloroplast late embryogenesis abundant-like proteins. Molecular characterization and functional analyses.

    abstract::Cold acclimation and freezing tolerance are the result of complex interaction between low temperature, light, and photosystem II (PSII) excitation pressure. Previous results have shown that expression of the Wcs19 gene is correlated with PSII excitation pressure measured in vivo as the relative reduction state of PSII...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: NDong C,Danyluk J,Wilson KE,Pocock T,Huner NP,Sarhan F

    更新日期:2002-07-01 00:00:00

  • Enzymes related to lactate metabolism in green algae and lower land plants.

    abstract::Cell-free extracts of Chlorella pyrenoidosa contained two enzymes capable of oxidizing d-lactate; these were glycolate dehydrogenase and NAD(+)-dependent d-lactate dehydrogenase. The two enzymes could be distinguished by differential centrifugation, glycolate dehydrogenase being largely particulate and NAD(+)-d-lactat...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Gruber PJ,Frederick SE,Tolbert NE

    更新日期:1974-02-01 00:00:00

  • Combining experimental and predicted datasets for determination of the subcellular location of proteins in Arabidopsis.

    abstract::Substantial experimental datasets defining the subcellular location of Arabidopsis (Arabidopsis thaliana) proteins have been reported in the literature in the form of organelle proteomes built from mass spectrometry data (approximately 2,500 proteins). Subcellular location for specific proteins has also been published...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Heazlewood JL,Tonti-Filippini J,Verboom RE,Millar AH

    更新日期:2005-10-01 00:00:00

  • Cytochrome c is released in a reactive oxygen species-dependent manner and is degraded via caspase-like proteases in tobacco Bright-Yellow 2 cells en route to heat shock-induced cell death.

    abstract::To gain some insight into the mechanism of plant programmed cell death, certain features of cytochrome c (cyt c) release were investigated in heat-shocked tobacco (Nicotiana tabacum) Bright-Yellow 2 cells in the 2- to 6-h time range. We found that 2 h after heat shock, cyt c is released from intact mitochondria into t...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Vacca RA,Valenti D,Bobba A,Merafina RS,Passarella S,Marra E

    更新日期:2006-05-01 00:00:00

  • The Chickpea Early Flowering 1 (Efl1) Locus Is an Ortholog of Arabidopsis ELF3.

    abstract::In climates that experience short growing seasons due to drought, heat, or end-of-season frost, early flowering is a highly desirable trait for chickpea (Cicer arietinum). In this study, we mapped, sequenced, and characterized Early flowering3 (Efl3), an ortholog of Arabidopsis (Arabidopsis thaliana) EARLY FLOWERING3 ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Ridge S,Deokar A,Lee R,Daba K,Macknight RC,Weller JL,Tar'an B

    更新日期:2017-10-01 00:00:00

  • CYP79D73 Participates in Biosynthesis of Floral Scent Compound 2-Phenylethanol in Plumeria rubra.

    abstract::Plumeria (Plumeria rubra), well known for its brightly colored and fragrant flowers, emits a number of floral volatile organic compounds (VOCs). Plumeria flowers emit a total of 43 VOCs including nine phenylpropanoids/benzenoids, such as 2-phenylethanol (2PE), benzaldehyde, 2-phenylacetaldehyde (PAld), (E/Z)-phenylace...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Dhandapani S,Jin J,Sridhar V,Chua NH,Jang IC

    更新日期:2019-05-01 00:00:00


    abstract::The presence of chloroplasts in guard cells from leaf epidermis, coleoptile, flowers, and albino portions of variegated leaves was established by incident fluorescence microscopy, thus confirming the notion that guard cell chloroplasts are remarkably conserved. Room temperature emission spectra from a few chloroplasts...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Zeiger E,Armond P,Melis A

    更新日期:1981-01-01 00:00:00

  • Chlorophyll synthesis in dark-grown pine primary needles

    abstract::The pigment content of dark-grown primary needles of Pinus jeffreyi L. and Pinus sylvestris L. was determined by high-performance liquid chromatography. The state of protochlorophyllide a and of chlorophylls during dark growth were analyzed by in situ 77 K fluorescence spectroscopy. Both measurements unambiguously dem...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Schoefs B,Franck F

    更新日期:1998-12-01 00:00:00

  • The physical basis of gravity stimulus nullification by clinostat rotation.

    abstract::The question of how rotation on a horizontal axis clinostat removes plants from the influence of the gravitational stimulus is answered. It is shown that appropriate horizontal axis clinostat rotation restricts the fall of intracellular particles to a quasi-circular path such that the position of the particle remains ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Dedolph RR,Dipert MH

    更新日期:1971-06-01 00:00:00

  • Transcription Factor bHLH2 Represses CYSTEINE PROTEASE77 to Negatively Regulate Nodule Senescence.

    abstract::Legume-rhizobia symbiosis is a time-limited process due to the onset of senescence, which results in the degradation of host plant cells and symbiosomes. A number of transcription factors, proteases, and functional genes have been associated with nodule senescence; however, whether other proteases or transcription fac...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Deng J,Zhu F,Liu J,Zhao Y,Wen J,Wang T,Dong J

    更新日期:2019-12-01 00:00:00

  • Aleurones from a Barley with Low [alpha]-Amylase Activity Become Highly Responsive to Gibberellin When Detached from the Starchy Endosperm.

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    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Skadsen RW

    更新日期:1993-05-01 00:00:00

  • Organization of monoterpene biosynthesis in Mentha. Immunocytochemical localizations of geranyl diphosphate synthase, limonene-6-hydroxylase, isopiperitenol dehydrogenase, and pulegone reductase.

    abstract::We present immunocytochemical localizations of four enzymes involved in p-menthane monoterpene biosynthesis in mint: the large and small subunits of peppermint (Mentha x piperita) geranyl diphosphate synthase, spearmint (Mentha spicata) (-)-(4S)-limonene-6-hydroxylase, peppermint (-)-trans-isopiperitenol dehydrogenase...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Turner GW,Croteau R

    更新日期:2004-12-01 00:00:00

  • Five Nodulation Mutants of White Sweetclover (Melilotus alba Desr.) Exhibit Distinct Phenotypes Blocked at Root Hair Curling, Infection Thread Development, and Nodule Organogenesis.

    abstract::In an effort to obtain a developmental sequence of mutations in the Rhizobium-legume interaction within a single legume species, we have characterized the early events of nodule development in 10 nodulation mutants of sweetclover, Melilotus alba Desr. cv U389, representing five genetic loci. Both seed and root exudate...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Utrup LJ,Cary AJ,Norris JH

    更新日期:1993-11-01 00:00:00

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    abstract::Gibberellic acid (GA) enhances the synthesis of alpha-amylase and ribonuclease in isolated aleurone layers and this process is inhibited by abscisin. Removal of gibberellic acid in mid-course of alpha-amylase production results in a slowing down of alpha-amylase synthesis, suggesting a continued requirement of GA for ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Chrispeels MJ,Varner JE

    更新日期:1967-07-01 00:00:00

  • Starch Turnover and Metabolism during Flower and Early Embryo Development.

    abstract::The accumulation of starch within photosynthetic tissues and within dedicated storage organs has been characterized extensively in many species, and a function in buffering carbon availability or in fueling later growth phases, respectively, has been proposed. However, developmentally regulated starch turnover within ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hedhly A,Vogler H,Schmid MW,Pazmino D,Gagliardini V,Santelia D,Grossniklaus U

    更新日期:2016-12-01 00:00:00


    abstract::The relationship between net photosynthesis and CO(2) concentration was investigated for four species of lichen using an infrared gas analyzer operating in a closed loop system. All species showed a linear relationship at low CO(2) levels (100 microliters per liter) with CO(2) saturation levels being in excess of 400 ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Green TG,Snelgar WP

    更新日期:1981-07-01 00:00:00

  • Passive nitrate transport by root plasma membrane vesicles exhibits an acidic optimal pH like the H(+)-ATPase.

    abstract::The net initial passive flux (J(Ni)) in reconstituted plasma membrane (PM) vesicles from maize (Zea mays) root cells was measured as recently described (P. Pouliquin, J.-P. Grouzis, R. Gibrat ¿1999 Biophys J 76: 360-373). J(Ni) in control liposomes responded to membrane potential or to NO(3)(-) as expected from the Go...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Pouliquin P,Boyer JC,Grouzis JP,Gibrat R

    更新日期:2000-01-01 00:00:00

  • Bound auxin metabolism in cultured crown-gall tissues of tobacco.

    abstract::Bound auxin metabolism in cultured crown-gall tumor cells and pith callus of tobacco was examined by feeding radiolabeled auxins and auxin conjugates. In all tissues fed [(14)C]indoleacetic acid (IAA), at least one-third of the IAA was decarboxylated, and most of the remaining radiolabel occurred in a compound(s) whic...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Vijayaraghavan SJ,Pengelly WL

    更新日期:1986-02-01 00:00:00

  • Nonadditive expression and parent-of-origin effects identified by microarray and allele-specific expression profiling of maize endosperm.

    abstract::Plant endosperm cells have a nuclear ratio of two maternal genomes to one paternal genome. This 2 to 1 dosage relationship provides a unique system for studying the additivity of gene expression levels in reciprocal hybrids. A combination of microarray profiling and allele-specific expression analysis was performed us...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Stupar RM,Hermanson PJ,Springer NM

    更新日期:2007-10-01 00:00:00

  • Isolation and Freezing Tolerances of Mesophyll Cells from Cold-hardened and Nonhardened Winter Rye.

    abstract::A method is described for the release of large quantities of mesophyll cells from leaves of both cold-hardened and nonhardened winter rye seedlings by a combination of enzymic and physical maceration. Such preparations usually contain a certain percentage of nonviable cells and are thus not suitable for quantitative b...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Singh J

    更新日期:1981-05-01 00:00:00

  • Characterization of P69E and P69F, two differentially regulated genes encoding new members of the subtilisin-like proteinase family from tomato plants.

    abstract::Subtilisin-like proteins represent an ancient family of serine proteases that are extremely widespread in living organisms. We report here the structure and genomic organization of two new transcriptionally active genes encoding proteins that belong to the P69 family of subtilisin-like proteases from tomato (Lycopersi...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Jordá L,Conejero V,Vera P

    更新日期:2000-01-01 00:00:00

  • Two young MicroRNAs originating from target duplication mediate nitrogen starvation adaptation via regulation of glucosinolate synthesis in Arabidopsis thaliana.

    abstract::Nitrogen is an essential macronutrient required for plant growth and development. A number of genes respond to nitrogen starvation conditions. However, the functions of most of these nitrogen starvation-responsive genes are unclear. Our recent survey suggested that many microRNAs (miRNAs) are responsive to nitrogen st...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: He H,Liang G,Li Y,Wang F,Yu D

    更新日期:2014-02-01 00:00:00

  • Cloning and functional expression of a cytochrome P450 cDNA encoding 2-hydroxyisoflavanone synthase involved in biosynthesis of the isoflavonoid skeleton in licorice.

    abstract::Isoflavonoids are distributed predominantly in leguminous plants and play critical roles in plant physiology. A cytochrome P450 (P450), 2-hydroxyisoflavanone synthase, is the key enzyme in their biosynthesis. In cultured licorice (Glycyrrhiza echinata L., Fabaceae) cells, the production of both an isoflavonoid-derived...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Akashi T,Aoki T,Ayabe Si

    更新日期:1999-11-01 00:00:00

  • Modification of the Cellular Heat Sensitivity of Cucumber by Growth under Supplemental Ultraviolet-B Radiation.

    abstract::The effect of ultraviolet B (UV-B) radiation on the thermal sensitivity of cucumber (Cucumis sativus L.) was studied using UV-B-sensitive cv Poinsett 76 and UV-B-resistant cv Ashley grown under control and elevated (300 mW m-2) UV-B radiation levels. Using both cotyledon and leaf discs, the ability of the tissue to re...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Caldwell CR

    更新日期:1994-02-01 00:00:00

  • Osmosensitive changes of carbohydrate metabolism in response to cellulose biosynthesis inhibition.

    abstract::Cellulose is the most abundant biopolymer in the world, the main load-bearing element in plant cell walls, and represents a major sink for carbon fixed during photosynthesis. Previous work has shown that photosynthetic activity is partially regulated by carbohydrate sinks. However, the coordination of cellulose biosyn...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wormit A,Butt SM,Chairam I,McKenna JF,Nunes-Nesi A,Kjaer L,O'Donnelly K,Fernie AR,Woscholski R,Barter MC,Hamann T

    更新日期:2012-05-01 00:00:00

  • Monochromatic light saturation curves for photosynthesis in Chlorella.

    abstract::We used a small oxygen electrode and a grating monochromator of 10 mmu half-band width to determine light-saturation curves of photosynthesis for films of Chlorella pyrenoidosa no more than 1 cell thick. All curves were referenced to the lightsaturated rate observed in 5 mw/cm(2) of 680 mmu. To a close approximation (...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Pickett JM,Myers J

    更新日期:1966-01-01 00:00:00

  • The Auxin Transport Inhibitor N-(1-Naphthyl)phthalamic Acid Elicits Pseudonodules on Nonnodulating Mutants of White Sweetclover.

    abstract::The collection of symbiotic (sym) mutants of white sweetclover (Melilotus alba Desr.) provides a developmental sequence of mutants blocked early in infection or nodule organogenesis. Mutant phenotypes include non-nodulating mutants that exhibit root-hair deformations in response to Rhizobium meliloti, mutants that for...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wu C,Dickstein R,Cary AJ,Norris JH

    更新日期:1996-02-01 00:00:00

  • Chemical and Biophysical Changes in the Plasma Membrane during Cold Acclimation of Mulberry Bark Cells (Morus bombycis Koidz. cv Goroji).

    abstract::The lipid and protein composition of the plasma membrane isolated from mulberry (Morus bombycis Koidz.) bark cells was analyzed throughout the cold acclimation period under natural and controlled environment conditions. There was a significant increase in phospholipids and unsaturation of their fatty acids during cold...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Yoshida S

    更新日期:1984-09-01 00:00:00

  • Transmission of the Monocarpic Senescence Signal via the Xylem in Soybean.

    abstract::During monocarpic senescence in soybean (Glycine max [L.] Merrill cv. Anoka) there is a remobilization of nitrogen from the leaves to the seeds, and it has been hypothesized that this loss of nitrogen from the leaves induces foliar yellowing. The phloem in a small segment of the petiole between the pods and the target...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Noodén LD,Murray BJ

    更新日期:1982-04-01 00:00:00