Abstract:
:Using the gibberellin (GA) biosynthetic inhibitor Uniconazol, we determined that det1, a mutant that no longer requires light to be germinated, still requires GA synthesis for germination. This result suggests that dark inhibition of germination in Arabidopsis may be due to inhibition of GA synthesis by the DET1 gene product in mature wild-type seeds. Similar experiments with mutants that lack seed dormancy due to a reduced sensitivity to abscisic acid (abi) have shown that abi1 and abi3 no longer require GA for germination. Furthermore, by shifting wild-type seeds to inhibitor at 6-hour intervals during imbibition, we determined that GA synthesis is only required during the first 24 hours of the imbibition process to reverse abscisic acid-induced dormancy in Arabidopsis.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Nambara E,Akazawa T,McCourt Pdoi
10.1104/pp.97.2.736subject
Has Abstractpub_date
1991-10-01 00:00:00pages
736-8issue
2eissn
0032-0889issn
1532-2548journal_volume
97pub_type
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