Abstract:
:Nodulation is crucial for biological nitrogen fixation (BNF) in legumes, but the molecular mechanisms underlying BNF have remained elusive. Here, we cloned a candidate gene underlying a major nodulation quantitative trait locus in soybean (Glycine max), INCREASING NODULE SIZE1 (GmINS1). GmINS1 encodes a cell wall β-expansin and is expressed primarily in vascular bundles, along with cortical and parenchyma cells of nodules. Four single-nucleotide polymorphisms distinguishing the two parents were found in the GmINS1 promoter region. Among them, single-nucleotide polymorphism A/C has a significant effect on GmINS1 expression in the parental genotype P2, based on β-glucuronidase activity and promoter deletion analysis. The expression of GmINS1 and the P2 genotype promoter was strongly associated with nodule development, not only in the parents but also in 40 progeny lines and 40 genotypes selected from a soybean core collection. Overexpression of GmINS1 resulted in increases in the number, biomass, infection cell abundance, and nitrogenase activity of large nodules and subsequently changed the nitrogen content and biomass of soybean plants. GmINS1 suppression via RNA interference had the opposite effect. Double suppression of GmEXPB2 and GmINS1 dramatically inhibited soybean nodulation. Our results reveal that GmINS1 is a critical gene in nodule development and that GmEXPB2 and GmINS1 synergistically control nodulation in soybean. Our findings shed light on the genetic basis of soybean nodulation and provide a candidate gene for optimizing BNF capacity through molecular breeding in soybean.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Li X,Zheng J,Yang Y,Liao Hdoi
10.1104/pp.18.01018subject
Has Abstractpub_date
2018-11-01 00:00:00pages
1233-1248issue
3eissn
0032-0889issn
1532-2548pii
pp.18.01018journal_volume
178pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Plant roots show a particularly high variation in their morphological response to different nutrient deficiencies. Although such changes often determine the nutrient efficiency or stress tolerance of plants, it is surprising that a comprehensive and comparative analysis of root morphological responses to different nut...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.218453
更新日期:2013-09-01 00:00:00
abstract::Plant hormones modulate plant growth, development, and defense. However, many aspects of the origin and evolution of plant hormone signaling pathways remain obscure. Here, we use a comparative genomic and phylogenetic approach to investigate the origin and evolution of nine major plant hormone (abscisic acid, auxin, b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.247403
更新日期:2015-03-01 00:00:00
abstract::The production of anthocyanins in fruit tissues is highly controlled at the developmental level. We have studied the expression of flavonoid biosynthesis genes during the development of bilberry (Vaccinium myrtillus) fruit in relation to the accumulation of anthocyanins, proanthocyanidins, and flavonols in wild berrie...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.006957
更新日期:2002-10-01 00:00:00
abstract::A method to fractionate corn (Zea mays L. B73) mitochondria into soluble proteins, high molecular weight soluble proteins, and membrane proteins was developed. These fractions were analyzed by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis and assays of mitochondrial enzyme activities. The Krebs cycle ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.4.1381
更新日期:1991-12-01 00:00:00
abstract::Improving the performance of rice (Oryza sativa) under drought stress has the potential to significantly affect rice productivity. Here, we report that the ERF family transcription factor OsLG3 positively regulates drought tolerance in rice. In our previous work, we found that OsLG3 has a positive effect on rice grain...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01492
更新日期:2018-09-01 00:00:00
abstract::Kinetic aspects of ethylene-mediated signal transduction leading to seedling-growth inhibition and chitinase induction in Arabidopsis were investigated by the introduction of defined mutations in components of these pathways. Dose-response analysis of wild-type responses indicated that the rate-limiting steps for seed...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.2.597
更新日期:1995-06-01 00:00:00
abstract::The effect of the exogenous and endogenous NO(3) (-) concentration on net uptake, influx, and efflux of NO(3) (-) and on nitrate reductase activity (NRA) in roots was studied in Phaseolus vulgaris L. cv. Witte Krombek. After exposure to NO(3) (-), an apparent induction period of about 6 hours occurred regardless of th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.3.754
更新日期:1982-09-01 00:00:00
abstract::Pollen tube growth requires a Ca2+ gradient, with elevated levels of cytosolic Ca2+ at the growing tip. This gradient's magnitude oscillates with growth oscillation but is always maintained. Ca2+ influx into the growing tip is necessary, and its magnitude also oscillates with growth. It has been widely assumed that st...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.041483
更新日期:2004-07-01 00:00:00
abstract::Selenium-Binding Protein1 (SBP1) gene expression was studied in Arabidopsis (Arabidopsis thaliana) seedlings challenged with several stresses, including cadmium (Cd), selenium {selenate [Se(VI)] and selenite [Se(IV)]}, copper (Cu), zinc (Zn), and hydrogen peroxide (H(2)O(2)) using transgenic lines expressing the lucif...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.144808
更新日期:2009-10-01 00:00:00
abstract::In this communication we document the reproducible protocols for the purification of milligram quantities of cytochrome b(5) and NADH-cytochrome b(5) reductase from the microsomal fraction of Pisum sativum. The cytochrome b(5) component of this NADH linked electron transport chain was found to have a molecular mass of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.2.457
更新日期:1987-10-01 00:00:00
abstract::Changes in the levels of [(14)C]indole-3-acetic acid (IAA) and [(14)C]indole-acetylaspartic acid (IAAsp) were examined during adventitious root formation in mung bean (Vigna radiata [L.] R. Wilcz. ;Berken') stem cuttings. IAAsp was identified by GC-MS as the primary conjugate in IAA-treated cuttings. During root forma...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.4.1236
更新日期:1988-04-01 00:00:00
abstract::Exogenous application of gibberellic acid (GA(3)) was able to reverse the inhibitory effect of salt, oxidative, and heat stresses in the germination and seedling establishment of Arabidopsis (Arabidopsis thaliana), this effect being accompanied by an increase in salicylic acid (SA) levels, a hormone that in recent yea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.139352
更新日期:2009-07-01 00:00:00
abstract::Primary leaves of 8-day-old Phaseolus vulgaris plants dipped in water at 46.5 to 47.5 C for 2 minutes were found to have decreased synthesis of lipid and beta-glucan compared to controls; UDP-glucose was used as substrate by cell-free preparations. As time elapsed, leaf growth, which was inhibited within the first 12 ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.1.118
更新日期:1974-01-01 00:00:00
abstract::Ecophysiological models predict quantitative traits of one genotype in any environment, whereas quantitative trait locus (QTL) models predict the contribution of alleles to quantitative traits under a limited number of environments. We have combined both approaches by dissecting into effects of QTLs the parameters of ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013839
更新日期:2003-02-01 00:00:00
abstract::MicroRNAs (miRNAs) are approximately 21-nucleotide-long RNAs processed from nuclear-encoded transcripts, which include a characteristic hairpin-like structure. MiRNAs control the expression of target transcripts by binding to reverse complementary sequences directing cleavage or translational inhibition of the target ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.128025
更新日期:2008-10-01 00:00:00
abstract::While it is apparent that the heat shock response is ubiquitous, variabilities in the nature of the heat shock response between closely related species have not been well characterized. The heat shock response of three genotypes of tomato, Lycopersicon esculentum, Lycopersicon pennellii, and the interspecific sexual h...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.3.1140
更新日期:1990-07-01 00:00:00
abstract::The intercellular washing fluid (IWF) from leaves of sugar beet (Beta vulgaris L.) contains a number of proteins exhibiting in vitro antifungal activity against the devastating leaf pathogen Cercospora beticola (Sacc.). Among these, a potent antifungal peptide, designated IWF4, was identified. The 30-amino-acid residu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.1.83
更新日期:1997-01-01 00:00:00
abstract::Leaf tissues from Xerosicyos danguyi H. Humb., a succulent member of the Cucurbitaceae, were found to possess both galactinol synthase activity and the capacity for photosynthetic production of stachyose, the phloem transport oligosaccharide common to other nonsucculent cucurbits. The amounts of stachyose isolated fro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.3.588
更新日期:1988-07-01 00:00:00
abstract::We conducted a comprehensive metabolic phenotyping of potato (Solanum tuberosum L. cv Desiree) tuber tissue that had been modified either by transgenesis or exposure to different environmental conditions using a recently developed gas chromatography-mass spectrometry profiling protocol. Applying this technique, we wer...
journal_title:Plant physiology
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
abstract::The structure of phosphatidylinositol in barley (Hordeum vulgare) aleurone layers was investigated by chemical degradation. In vivo myo-[2-(3)H]inositol-labeled phosphatidylinositol was first converted to glycerophosphoinositol and, subsequently, after removal of the glycerol moiety, to inositol monophosphate. Here, w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.4.1498
更新日期:1992-04-01 00:00:00
abstract::A new bacterial agglutinin was isolated from seeds of the soybean cultivar Clark. Purification was carried out by ammonium sulfate precipitation and ion-exchange chromatography. The agglutinin is a heat-labile glycoprotein most active at pH 4.0. Addition of Ca(2+), Mn(2+) and Mg(2+) did not enhance the agglutinating a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.5.847
更新日期:1980-11-01 00:00:00
abstract::When petunia (Petunia hybrida Vilm, cv Rosy Morn) cells are cultured in the presence of 2 [mu]M antimycin A (AA), respiration proceeds mainly via the cyanide-resistant pathway. Cyanide-resistant respiratory rates were higher in mitochondria from AA cells than in control mitochondria. Compared with control cells, an in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.2.617
更新日期:1997-10-01 00:00:00
abstract::The effect of Co(2+) on ethylene production by mung bean (Phaseolus aureus Roxb.) and by apple tissues was studied. Co(2+), depending on concentrations applied, effectively inhibited ethylene production by both tissues. It also strongly inhibited the ethylene production induced by IAA, kinetin, IAA plus kinetin, Ca(2+...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.1.114
更新日期:1976-07-01 00:00:00
abstract::Although cytokinins (CKs) affect a number of processes connected with chloroplasts, it has never been rigorously proven that chloroplasts contain CKs. We isolated intact chloroplasts from tobacco (Nicotiana tabacum L. cv SR1) and wheat (Triticum aestivum L. cv Ritmo) leaves and determined their CKs by liquid chromatog...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.1.245
更新日期:1999-09-01 00:00:00
abstract::Spinach (Spinacia oleracea CV Bloomsdale Long Standing) leaf cytoplasmic starch phosphorylase and rabbit muscle phosphorylase a were inactivated by incubation with partially purified leaf extract in the presence of ATP and Mg(2+). The inactivating factor(s) were heat stable and susceptible to protease attack. Phosphor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.4.902
更新日期:1983-12-01 00:00:00
abstract::Symbioses between plants and mycorrhizal fungi are ubiquitous in ecosystems and strengthen the plants' defense against aboveground herbivores. Here, we studied the underlying regulatory networks and biochemical mechanisms in leaves induced by ectomycorrhizae that modify herbivore interactions. Feeding damage and ovipo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01810
更新日期:2018-04-01 00:00:00
abstract::The triple isotope composition (delta17O and delta18O) of dissolved O2 in the ocean and in ice cores was recently used to assess the primary productivity over broad spatial and temporal scales. However, assessment of the productivity with the aid of this method must rely on accurate measurements of the 17O/16O versus ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.063768
更新日期:2005-08-01 00:00:00
abstract::Using tissue blotting and immunocytolocalization, we have investigated the appearance and accumulation of polygalacturonase (PG) during tomato (Lycopersicon esculentum Mill.) fruit ripening. Results show that PG first appears in the collumella region followed by sequential appearance in the exopericarp and endopericar...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.1.17
更新日期:1989-05-01 00:00:00
abstract::Our results show that the phosphate ion is a nonlinear competitive inhibitor of Pinus pinea isocitrate lyase. In addition, this compound induces a sigmoidal response of the enzyme, which usually exhibits standard Michaelis-Menten kinetics. This peculiar behavior of P. pinea isocitrate lyase could be explained by a dim...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.124.3.1131
更新日期:2000-11-01 00:00:00
abstract::The amination of alpha-ketoglutarate (alpha-KG) by NADH-glutamate dehydrogenase (GDH) obtained from Sephadex G-75 treated crude extracts from shoots of 5-day-old seedlings was stimulated by the addition of Ca(2+). The NADH-GDH purified 161-fold with ammonium sulfate, DEAE-Toyopearl, and Sephadex G-200 was also activat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.4.1009
更新日期:1984-12-01 00:00:00