Effect of exogenous and endogenous nitrate concentration on nitrate utilization by dwarf bean.

Abstract:

:The effect of the exogenous and endogenous NO(3) (-) concentration on net uptake, influx, and efflux of NO(3) (-) and on nitrate reductase activity (NRA) in roots was studied in Phaseolus vulgaris L. cv. Witte Krombek. After exposure to NO(3) (-), an apparent induction period of about 6 hours occurred regardless of the exogenous NO(3) (-) level. A double reciprocal plot of the net uptake rate of induced plants versus exogenous NO(3) (-) concentration yielded four distinct phases, each with simple Michaelis-Menten kinetics, and separated by sharp breaks at about 45, 80, and 480 micromoles per cubic decimeter.Influx was estimated as the accumulation of (15)N after 1 hour exposure to (15)NO(3) (-). The isotherms for influx and net uptake were similar and corresponded to those for alkali cations and Cl(-). Efflux of NO(3) (-) was a constant proportion of net uptake during initial NO(3) (-) supply and increased with exogenous NO(3) (-) concentration. No efflux occurred to a NO(3) (-)-free medium.The net uptake rate was negatively correlated with the NO(3) (-) content of roots. Nitrate efflux, but not influx, was influenced by endogenous NO(3) (-). Variations between experiments, e.g. in NO(3) (-) status, affected the values of K(m) and V(max) in the various concentration phases. The concentrations at which phase transitions occurred, however, were constant both for influx and net uptake. The findings corroborate the contention that separate sites are responsible for uptake and transitions between phases.Beyond 100 micromoles per cubic decimeter, root NRA was not affected by exogenous NO(3) (-) indicating that NO(3) (-) uptake was not coupled to root NRA, at least not at high concentrations.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Breteler H,Nissen P

doi

10.1104/pp.70.3.754

subject

Has Abstract

pub_date

1982-09-01 00:00:00

pages

754-9

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

70

pub_type

杂志文章
  • Plasma Membrane Redox Enzyme Is Involved in the Synthesis of O2- and H2O2 by Phytophthora Elicitor-Stimulated Rose Cells.

    abstract::An elicitor prepared from the autoclaved cell walls of Phytophthora sp. induced O2- generation and H2O2 accumulation by cultured cells of Rosa damascena Mill. cv Gloire de Guilan. N,N-Diethyldithiocarbamate, a superoxide dismutase inhibitior, blocked H2O2 accumulation and caused a dramatic accumulation of O2- by elici...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.4.1241

    authors: Auh CK,Murphy TM

    更新日期:1995-04-01 00:00:00

  • Effects of the gibberellin biosynthetic inhibitor uniconazol on mutants of Arabidopsis.

    abstract::Using the gibberellin (GA) biosynthetic inhibitor Uniconazol, we determined that det1, a mutant that no longer requires light to be germinated, still requires GA synthesis for germination. This result suggests that dark inhibition of germination in Arabidopsis may be due to inhibition of GA synthesis by the DET1 gene ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.2.736

    authors: Nambara E,Akazawa T,McCourt P

    更新日期:1991-10-01 00:00:00

  • Tobacco Plants Transformed with the Bean alphaai Gene Express an Inhibitor of Insect alpha-Amylase in Their Seeds.

    abstract::Bean (Phaseolus vulgaris L.) seeds contain a putative plant defense protein that inhibits insect and mammalian but not plant alpha-amylases. We recently (J Moreno, MJ Chrispeels [1989] Proc Natl Acad Sci USA 86:7885-7889) presented strong circumstantial evidence that this alpha-amylase inhibitor (alphaAI) is encoded b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.805

    authors: Altabella T,Chrispeels MJ

    更新日期:1990-06-01 00:00:00

  • Ion fluxes and abscisic Acid-induced proline accumulation in barley leaf segments.

    abstract::The increase in proline induced by ABA, a process stimulated by NaCl or KCl in barley leaves, did not occur when Na(+) (or K(+)) was present in the external medium as the gluconate salt, namely with an anion unable to permeate the plasma membrane. However, proline increase was restored, to different extents, by the ad...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.3.927

    authors: Pesci P

    更新日期:1988-03-01 00:00:00

  • Environmental Parameters Affecting Dark Response of Rice Seedlings (Oryza sativa L.) to Triacontanol.

    abstract::Triacontanol applied to IR-8 rice (Oryza sativa L.) seedlings in nutrient solution caused an increase in dry weight during a 6-hour dark period. This increase was altered by atmospheric CO(2) and O(2) concentrations. The largest growth response occurred from 200 to 350 muliters/liter CO(2) with 5% O(2). The treated se...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.5.851

    authors: Bittenbender HC,Dilley DR,Wert V,Ries SK

    更新日期:1978-05-01 00:00:00

  • Expression, activity, and cellular accumulation of methyl jasmonate-responsive lipoxygenase in soybean seedlings.

    abstract::Exposure of soybean (Glycine max) seedlings to low levels of atmospheric methyl jasmonate induced the expression and accumulation of one or more lipoxygenase(s) in the primary leaves, hypocotyls, epicotyls, and cotyledons. In the primary leaf, the major site of lipoxygenase accumulation in response to methyl jasmonate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.1.433

    authors: Grimes HD,Koetje DS,Franceschi VR

    更新日期:1992-09-01 00:00:00

  • The Non-Mendelian Green Cotyledon Gene in Soybean Encodes a Small Subunit of Photosystem II.

    abstract::Chlorophyll degradation plays important roles in leaf senescence including regulation of degradation of chlorophyll-binding proteins. Although most genes encoding enzymes of the chlorophyll degradation pathway have been identified, the regulation of their activity has not been fully understood. Green cotyledon mutants...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01589

    authors: Kohzuma K,Sato Y,Ito H,Okuzaki A,Watanabe M,Kobayashi H,Nakano M,Yamatani H,Masuda Y,Nagashima Y,Fukuoka H,Yamada T,Kanazawa A,Kitamura K,Tabei Y,Ikeuchi M,Sakamoto W,Tanaka A,Kusaba M

    更新日期:2017-04-01 00:00:00

  • Senescence: action of auxin and kinetin in control of RNA and protein synthesis in subcellular fractions of bean endocarp.

    abstract::A comparative study was made of the effects of auxin (alpha-naphthalene acetic acid), kinetin (6-furfurylaminopurine) and a mixture of auxin and kinetin applied in vivo on synthesis of RNA and protein and the distribution of such synthesis amongst the subcellular fractions of sections of endocarp from Kentucky Wonder ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.10.1334

    authors: Sacher JA

    更新日期:1967-10-01 00:00:00

  • Effects of water stress on the organic Acid and carbohydrate compositions of cotton plants.

    abstract::Two photoperiodic cotton (Gossypium hirsutum L.) strains (T185 and T466) which had been empirically selected because of poor performance and two strains (T25 and T256) selected because of enhanced performance under field water stress were evaluated for stress-induced changes in their organic acids and carbohydrates. P...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.3.724

    authors: Timpa JD,Burke JJ,Quisenberry JE,Wendt CW

    更新日期:1986-11-01 00:00:00

  • C4 Photosynthetic Gene Expression in Light- and Dark-Grown Amaranth Cotyledons.

    abstract::The patterns of expression for genes encoding several C4 photosynthetic enzymes were examined in light-grown and dark-grown (etiolated) cotyledons of amaranth (Amaranthus hypochondriacus), a dicotyledonous C4 plant. The large subunit and small subunit of ribulose-1,5-bisphosphate carboxylase (RuBPCase), phosphoenolpyr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.4.1085

    authors: Wang JL,Long JJ,Hotchkiss T,Berry JO

    更新日期:1993-08-01 00:00:00

  • Activation of a Reserve Pool of Photosystem II in Chlamydomonas reinhardtii Counteracts Photoinhibition.

    abstract::The effect of strong irradiance (2000 micromole photons per square meter per second) on PSII heterogeneity in intact cells of Chlamydomonas reinhardtii was investigated. Low light (LL, 15 micromole photons per square meter per second) grown C. reinhardtii are photoinhibited upon exposure to strong irradiance, and the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.92.4.1196

    authors: Neale PJ,Melis A

    更新日期:1990-04-01 00:00:00

  • Molecular cloning of a {beta}-galactosidase from radish that specifically hydrolyzes {beta}-(1->3)- and {beta}-(1->6)-galactosyl residues of Arabinogalactan protein.

    abstract::A basic beta-galactosidase with high specificity toward beta-(1-->3)- and beta-(1-->6)-galactosyl residues was cloned from radish (Raphanus sativus) plants by reverse transcription-PCR. The gene, designated RsBGAL1, contained an open reading frame consisting of 2,532 bp (851 amino acids). It is expressed in hypocotyls...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.062562

    authors: Kotake T,Dina S,Konishi T,Kaneko S,Igarashi K,Samejima M,Watanabe Y,Kimura K,Tsumuraya Y

    更新日期:2005-07-01 00:00:00

  • The role of cyclic photophosphorylation in vivo.

    abstract::When cyclic photophosphorylation is inhibited in Chlorella vulgaris cells by carbonylcyanide-trifluoromethoxy phenylhy-drazone, photosynthetic CO(2)-fixation under anaerobic conditions exhibits a distinct lag. Under the same conditions, the light-dependent formation of ribulose diphosphate shows also this lag. It is c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.5.825

    authors: Klob W,Kandler O,Tanner W

    更新日期:1973-05-01 00:00:00

  • Salinity stress inhibits bean leaf expansion by reducing turgor, not wall extensibility.

    abstract::Treatment of bean (Phaseolus vulgaris L.) seedlings with low levels of salinity (50 or 100 millimolar NaCl) decreased the rate of light-induced leaf cell expansion in the primary leaves over a 3 day period. This decrease could be due to a reduction in one or both of the primary cellular growth parameters: wall exten...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.1.233

    authors: Neumann PM,Van Volkenburgh E,Cleland RE

    更新日期:1988-01-01 00:00:00

  • Reprogramming the phenylpropanoid metabolism in seeds of oilseed rape by suppressing the orthologs of reduced epidermal fluorescence1.

    abstract::As a result of the phenylpropanoid pathway, many Brassicaceae produce considerable amounts of soluble hydroxycinnamate conjugates, mainly sinapate esters. From oilseed rape (Brassica napus), we cloned two orthologs of the Arabidopsis (Arabidopsis thaliana) gene reduced epidermal fluorescence1 (REF1) encoding a conifer...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.215491

    authors: Mittasch J,Böttcher C,Frolov A,Strack D,Milkowski C

    更新日期:2013-04-01 00:00:00

  • Diversity of the superfamily of phloem lectins (phloem protein 2) in angiosperms.

    abstract::Phloem protein 2 (PP2) is one of the most abundant and enigmatic proteins in the phloem sap. Although thought to be associated with structural P-protein, PP2 is translocated in the assimilate stream where its lectin activity or RNA-binding properties can exert effects over long distances. Analyzing the diversity of th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.013086

    authors: Dinant S,Clark AM,Zhu Y,Vilaine F,Palauqui JC,Kusiak C,Thompson GA

    更新日期:2003-01-01 00:00:00

  • Properties and Ultrastructure of Phycoerythrin From Porphyridium cruentum.

    abstract::Phycoerythrin, a photosynthetic accessory pigment, was isolated from Porphyridium cruentum and examined by electron microscopy and disc gel electrophoresis. The absorption monomer, with maxima at 563, 545, and a shoulder at 500 nm, has a molecular weight of about 300,000. With phosphotungstic acid staining it appears ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.11.1629

    authors: Gantt E

    更新日期:1969-11-01 00:00:00

  • Messenger RNA-binding properties of nonpolysomal ribonucleoproteins from heat-stressed tomato cells

    abstract::Most cells experiencing heat stress reprogram their translational machinery to favor the synthesis of heat-stress proteins. Translation of other transcripts is almost completely repressed, but most untranslated messengers are not degraded. In contrast to yeast, Drosophila melanogaster, and HeLa cells, plant cells stor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.1.23

    authors: Stuger R,Ranostaj S,Materna T,Forreiter C

    更新日期:1999-05-01 00:00:00

  • Proteomic investigation of the effect of salicylic acid on Arabidopsis seed germination and establishment of early defense mechanisms.

    abstract::The influence of salicylic acid (SA) on elicitation of defense mechanisms in Arabidopsis (Arabidopsis thaliana) seeds and seedlings was assessed by physiological measurements combined with global expression profiling (proteomics). Parallel experiments were carried out using the NahG transgenic plants expressing the ba...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.082057

    authors: Rajjou L,Belghazi M,Huguet R,Robin C,Moreau A,Job C,Job D

    更新日期:2006-07-01 00:00:00

  • Quantitative changes in in vitro and in vivo protein synthesis in aging and rejuvenated soybean cotyledons.

    abstract::Cotyledons of light-grown soybean (Glycine max L. var Wayne) seedlings were used as a model system to study the possibility that aging requires qualitative changes in protein synthesis. Cotyledons reached a final stage of senescence and then abscised about 22 days after imbibition. Cotyledon senescence was reversed at...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.4.861

    authors: Skadsen RW,Cherry JH

    更新日期:1983-04-01 00:00:00

  • The small GTPase ROP6 interacts with NFR5 and is involved in nodule formation in Lotus japonicus.

    abstract::Nod Factor Receptor5 (NFR5) is an atypical receptor-like kinase, having no activation loop in the protein kinase domain. It forms a heterodimer with NFR1 and is required for the early plant responses to Rhizobium infection. A Rho-like small GTPase from Lotus japonicus was identified as an NFR5-interacting protein. The...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.197269

    authors: Ke D,Fang Q,Chen C,Zhu H,Chen T,Chang X,Yuan S,Kang H,Ma L,Hong Z,Zhang Z

    更新日期:2012-05-01 00:00:00

  • Inhibition and stimulation of root respiration in pisum and plantago by hydroxamate : its consequences for the assessment of alternative path activity.

    abstract::The contribution of the alternative pathway in root respiration of Pisum sativum L. cv Rondo, Plantago lanceolata L., and Plantago major L. ssp major was determined by titration with salicylhydroxamate (SHAM) in the absence and presence of cyanide. SHAM completely inhibited the cyanide-resistant component of root resp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.3.813

    authors: de Visser R,Blacquière T

    更新日期:1984-07-01 00:00:00

  • Does pollination induce corolla abscission of cyclamen flowers by promoting ethylene production?

    abstract::Very low ethylene production rates were measured in nonpollinated Cyclamen persicum Mill flowers, and no change in production was observed during the whole life span of the flower until death. Normal senescence was accompanied by a gradual discoloration and loss of turgor followed by wilting. Pollination induced a dra...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.4.1090

    authors: Halevy AH,Whitehead CS,Kofranek AM

    更新日期:1984-08-01 00:00:00

  • Glutamine synthetase-glutamate synthase pathway and glutamate dehydrogenase play distinct roles in the sink-source nitrogen cycle in tobacco.

    abstract::Glutamate (Glu) metabolism and amino acid translocation were investigated in the young and old leaves of tobacco (Nicotiana tabacum L. cv Xanthi) using [15N]ammonium and [2-15N]Glu tracers. Regardless of leaf age, [15N]ammonium assimilation occurred via glutamine synthetase (GS; EC 6.1.1.3) and Glu synthase (ferredoxi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.071910

    authors: Masclaux-Daubresse C,Reisdorf-Cren M,Pageau K,Lelandais M,Grandjean O,Kronenberger J,Valadier MH,Feraud M,Jouglet T,Suzuki A

    更新日期:2006-02-01 00:00:00

  • Cysteine transport into cultured tobacco cells.

    abstract::Cysteine transport by tobacco cells (Nicotiana tabacum L. var. Xanthi) cultured on liquid B-5 medium was examined.Transport was linear with time or amount of tissue and had a pH optimum of 4.5. Cysteine transport over a wide concentration range was biphasic. The isotherm, for descriptive convenience, was divided into ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.6.807

    authors: Harrington HM,Smith IK

    更新日期:1977-12-01 00:00:00

  • Effects of Sink Removal on Photosynthesis and Senescence in Leaves of Soybean (Glycine max L.) Plants.

    abstract::Photosynthetic rate, ribulose 1,5-bisphosphate carboxylase activity, specific leaf weight, and leaf concentrations of carbohydrates, proteins, chlorophyll, and inorganic phosphate were determined periodically from midbloom until maturity in leaves of soybean plants (Glycine max L., var. Hodgson) from which reproductiv...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.3.394

    authors: Mondal MH,Brun WA,Brenner ML

    更新日期:1978-03-01 00:00:00

  • TsHKT1;2, a HKT1 homolog from the extremophile Arabidopsis relative Thellungiella salsuginea, shows K(+) specificity in the presence of NaCl.

    abstract::Cellular Na(+)/K(+) ratio is a crucial parameter determining plant salinity stress resistance. We tested the function of plasma membrane Na(+)/K(+) cotransporters in the High-affinity K(+) Transporter (HKT) family from the halophytic Arabidopsis (Arabidopsis thaliana) relative Thellungiella salsuginea. T. salsuginea c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.193110

    authors: Ali Z,Park HC,Ali A,Oh DH,Aman R,Kropornicka A,Hong H,Choi W,Chung WS,Kim WY,Bressan RA,Bohnert HJ,Lee SY,Yun DJ

    更新日期:2012-03-01 00:00:00

  • Differential tomato transcriptomic responses induced by pepino mosaic virus isolates with differential aggressiveness.

    abstract::Pepino mosaic virus (PepMV) is a highly infectious potexvirus and a major disease of greenhouse tomato (Solanum lycopersicum) crops worldwide. Damage and economic losses caused by PepMV vary greatly and can be attributed to differential symptomatology caused by different PepMV isolates. Here, we used a custom-designed...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.173906

    authors: Hanssen IM,van Esse HP,Ballester AR,Hogewoning SW,Parra NO,Paeleman A,Lievens B,Bovy AG,Thomma BP

    更新日期:2011-05-01 00:00:00

  • Decreased Exopolysaccharide Synthesis by Anaerobic and Symbiotic Cells of Bradyrhizobium japonicum.

    abstract::Experiments were conducted to determine whether symbiotic bacteroids of Bradyrhizobium japonicum produce exopolysaccharide within soybean (Glycine max [L.] Merr. cv ;Lee 74') nodules. B. japonicum strains RT2, a derivative of USDA 110 with resistance to streptomycin and rifampicin, and RT176-1, a mutant deficient in e...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.2.445

    authors: Tully RE,Terry ME

    更新日期:1985-10-01 00:00:00

  • Nicotine Biosynthetic Enzyme Activities in Nicotiana tabacum L. Genotypes with Different Alkaloid Levels.

    abstract::Young plants of five Nicotiana tabacum L. genotypes were examined for activity of nicotine biosynthetic enzymes. Genotypes near isogenic except at two loci each with two alleles controlling nicotine level were used in a comparison of the four homozygous allelic combinations producing high, high intermediate, low inter...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.2.236

    authors: Saunders JW,Bush LP

    更新日期:1979-08-01 00:00:00