Quantitative changes in in vitro and in vivo protein synthesis in aging and rejuvenated soybean cotyledons.

abstract：:Using the gibberellin (GA) biosynthetic inhibitor Uniconazol, we determined that det1, a mutant that no longer requires light to be germinated, still requires GA synthesis for germination. This result suggests that dark inhibition of germination in Arabidopsis may be due to inhibition of GA synthesis by the DET1 gene ...

journal_title：Plant physiology

authors： Nambara E,Akazawa T,McCourt P

更新日期：1991-10-01 00:00:00

abstract：:We used an anti-indole acetic acid (IAA or auxin) monoclonal antibody-based immunocytochemical procedure to monitor IAA level in Arabidopsis tissues. Using immunocytochemistry and the IAA-driven beta-glucuronidase (GUS) activity of Aux/IAA promoter::GUS constructs to detect IAA distribution, we investigated the role o...

journal_title：Plant physiology

authors： Avsian-Kretchmer O,Cheng JC,Chen L,Moctezuma E,Sung ZR

更新日期：2002-09-01 00:00:00

abstract：:The intercellular washing fluid (IWF) from leaves of sugar beet (Beta vulgaris L.) contains a number of proteins exhibiting in vitro antifungal activity against the devastating leaf pathogen Cercospora beticola (Sacc.). Among these, a potent antifungal peptide, designated IWF4, was identified. The 30-amino-acid residu...

journal_title：Plant physiology

authors： Nielsen KK,Nielsen JE,Madrid SM,Mikkelsen JD

更新日期：1997-01-01 00:00:00

abstract：:The succulent leaf of the obligate Crassulacean acid metabolism plant Crassula falcata comprises two distinct types of parenchyma. The peripheral tissue is dark green, whereas the central tissue is relatively colorless. We have investigated whether the conventional interpretation of Crassulacean acid metabolism-simply...

journal_title：Plant physiology

authors： Springer SA,Outlaw WH

更新日期：1988-11-01 00:00:00

abstract：:The cytokinin activity has been isolated and identified from extracts of immature mango (Mangifera indica L.) seeds. The structures of zeatin, zeatin riboside, and N(6)-(Delta(2)-isopentenyl)adenine riboside were confirmed on the basis of their chromatographic behavior and mass spectra of trimethylsilyl derivatives. B...

journal_title：Plant physiology

authors： Chen WS

更新日期：1983-02-01 00:00:00

abstract：:The fluorescent compound trisodium, 3-hydroxy-5,8,10-pyrenetrisulfonate (PTS) was used to quantify the apoplastic flux through red pine (Pinus resinosa Ait.) root systems-that portion of the total water flux reaching the xylem without ever crossing a semipermeable membrane. Flow was induced by pressure through detoppe...

journal_title：Plant physiology

authors： Hanson PJ,Sucoff EI,Markhart AH

更新日期：1985-01-01 00:00:00

abstract：:A 3,300-bp DNA fragment encoding the carboxyl-transferase domain of the multidomain, chloroplastic acetyl-coenzyme A carboxylase (ACCase) was sequenced in aryloxyphenoxypropionate (APP)-resistant and -sensitive Alopecurus myosuroides (Huds.). No resistant plant contained an Ile-1,781-Leu substitution, previously shown...

journal_title：Plant physiology

authors： Délye C,Zhang XQ,Chalopin C,Michel S,Powles SB

更新日期：2003-07-01 00:00:00

abstract：:We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogena...

journal_title：Plant physiology

authors： Zhang C,Barthelson RA,Lambert GM,Galbraith DW

更新日期：2008-05-01 00:00:00

abstract：:The relationship between loss of ribulose-1,5-bisphosphate carboxylase (RuBPCase) and the decline in photosynthesis during the senescence of barley primary leaves was assessed. Loss of RuBPCase accounted for about 85% of the decrease in soluble protein. RuBPCase was highly correlated with in vitro RuBPCase activity (r...

journal_title：Plant physiology

authors： Friedrich JW,Huffaker RC

更新日期：1980-06-01 00:00:00

abstract：:Microarray data can be used to derive understanding of the relationships between the genes involved in various biological systems of an organism, given the availability of databases of gene expression measurements from the complete spectrum of experimental conditions and materials. However, there have been no reports,...

journal_title：Plant physiology

authors： Lee TH,Kim YK,Pham TT,Song SI,Kim JK,Kang KY,An G,Jung KH,Galbraith DW,Kim M,Yoon UH,Nahm BH

更新日期：2009-09-01 00:00:00

abstract：:An efficient negative selection procedure is crucial to the isolation of rare homologous recombinants in gene targeting. Although gene targeting is a common practice in lower eukaryotes and is becoming routine in mammals, its application to plants has not been achieved. In this report, we have evaluated an antisense c...

journal_title：Plant physiology

authors： Xiang C,Guerra DJ

更新日期：1993-05-01 00:00:00

abstract：:The relationship among physiological, injury, growth, and yield responses was examined in field-grown green pepper (Capsicum annuum L. ;California Wonder') subjected to two airborne environmental stresses. The primary objectives were to determine if the stresses could cause alterations in the plant responses, and to d...

journal_title：Plant physiology

authors： Takemoto BK,Bytnerowicz A,Olszyk DM

更新日期：1988-10-01 00:00:00

abstract：:In Solanaceae, the S-specific interaction between the pistil S-RNase and the pollen S-Locus F-box protein controls self-incompatibility (SI). Although this interaction defines the specificity of the pollen rejection response, the identification of three pistil essential modifier genes unlinked to the S-locus (HT-B, 12...

journal_title：Plant physiology

authors： García-Valencia LE,Bravo-Alberto CE,Wu HM,Rodríguez-Sotres R,Cheung AY,Cruz-García F

更新日期：2017-11-01 00:00:00

abstract：:We found inconsistencies in the commonly used data for chlorophyll analysis in 80% acetone. Recently developed extinction coefficients for chlorophyll b in N,N-dimethylformamide (DMF) based on values from 80% acetone are low as a result of these inconsistencies. We determined extinction coefficients of chlorophyll a (...

journal_title：Plant physiology

authors： Inskeep WP,Bloom PR

更新日期：1985-02-01 00:00:00

abstract：:An investigation was made to determine the univalent cation requirements of starch synthetase from a variety of plant species of economic importance. The particulate enzyme from sweet corn was shown to have an absolute requirement for potassium, with the optimum activation occurring at 0.05 M KCl. Rubidium, cesium, an...

journal_title：Plant physiology

authors： Nitsos RE,Evans HJ

更新日期：1969-09-01 00:00:00

abstract：:Using a novel screening procedure, we have selected a new class of mutant from the cyanobacterium Synechococcus PCC7942 that fails to adapt to growth at an extremely low inorganic carbon (Ci) concentration. The mutant (Tm17) reported in this study grows normally at or above air levels of CO2 (340 [mu]L L-1) but does n...

journal_title：Plant physiology

authors： Yu JW,Price GD,Badger MR

更新日期：1994-02-01 00:00:00

abstract：:The effects of oxidative stress on the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1.39) were studied in isolated chloroplasts from barley (Hordeum vulgare L. cv Angora). Active oxygen (AO) was generated by varying the light intensity, the oxygen concentration, or the addition of he...

journal_title：Plant physiology

authors： Desimone M,Henke A,Wagner E

更新日期：1996-07-01 00:00:00

abstract：:The metabolism of labeled indole-3-acetic acid (IAA-2-(14)C) was investigated in Parthenocissus tricuspidata crown gall callus tissue. After 48 hours incubation, 85 to 90% of the supplied IAA was taken up by the tissue, and of that taken up, about 45% was conjugated with five amino acids. The conjugates found were asp...

journal_title：Plant physiology

authors： Feung CS,Hamilton RH,Mumma RO

更新日期：1976-11-01 00:00:00

abstract：:More volatile organic carbon is lost from plants as isoprene than any other molecule. This flux of carbon to the atmosphere affects atmospheric chemistry and can serve as a substrate for ozone production in polluted air. Isoprene synthesis may help leaves cope with heatflecks and active oxygen species. Isoprene syntha...

journal_title：Plant physiology

authors： Wolfertz M,Sharkey TD,Boland W,Kühnemann F

更新日期：2004-08-01 00:00:00

abstract：:Signals can be perceived and amplified at the cell membrane by receptors coupled to the production of a variety of second messengers, including myoinositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)]. The myoinositol polyphosphate 5-phosphatases (5PTases; EC 3.1.3.56) comprise a large protein family that hydrolyzes 5-phospha...

journal_title：Plant physiology

authors： Gunesekera B,Torabinejad J,Robinson J,Gillaspy GE

更新日期：2007-03-01 00:00:00

abstract：:Aspen (Populus tremuloides Michx.) leaf extracts contain a newly discovered enzyme activity that catalyzes the magnesium ion-dependent elimination of diphosphate from dimethylallyl diphosphate with rearrangement to form isoprene (2-methyl, 1-3-butadiene). This isoprene synthase activity has been partially purified. Th...

journal_title：Plant physiology

authors： Silver GM,Fall R

更新日期：1991-12-01 00:00:00

abstract：:Sucrose gradient profiles of polyribosomes from the coleoptilar node region of seedlings of Zea mays L. were obtained without pelleting and redispersion of the particles. Water stress caused a shift of ribosomes from the polymeric to the monomeric form, starting about 30 minutes after stress initiation and when the wa...

journal_title：Plant physiology

authors： Hsiao TC

更新日期：1970-08-01 00:00:00

abstract：:Protein dephosphorylation by the serine/threonine protein phosphatase 2A (PP2A) modulates a broad array of cellular functions. PP2A normally acts as a heterotrimeric holoenzyme complex comprising a catalytic subunit bound by regulatory A and B subunits. Characterization of the regulatory A subunit isoforms (ROOTS CURL...

journal_title：Plant physiology

authors： Blakeslee JJ,Zhou HW,Heath JT,Skottke KR,Barrios JA,Liu SY,DeLong A

更新日期：2008-02-01 00:00:00

abstract：:Sucrose phosphate synthase and acid invertase activities in the mature leaves of roses (Rosa hybrida cv Golden Times) were greater in plants grown under a higher night temperature than under a lower temperature regime. In young shoots, the activity of acid invertase was promoted by the lower temperature while that of ...

journal_title：Plant physiology

authors： Khayat E,Zieslin N

更新日期：1987-06-01 00:00:00

abstract：:Phosphorus (P) remobilization in plants is required for continuous growth and development. The Arabidopsis (Arabidopsis thaliana) inorganic phosphate (Pi) transporter Pht1;5 has been implicated in mobilizing stored Pi out of older leaves. In this study, we used a reverse genetics approach to study the role of Pht1;5 i...

journal_title：Plant physiology

authors： Nagarajan VK,Jain A,Poling MD,Lewis AJ,Raghothama KG,Smith AP

更新日期：2011-07-01 00:00:00

abstract：:The occurrence of a photorespiratory 2-phosphoglycolate metabolism in cyanobacteria is not clear. In the genome of the cyanobacterium Synechocystis sp. strain PCC 6803, we have identified open reading frames encoding enzymes homologous to those forming the plant-like C2 cycle and the bacterial-type glycerate pathway. ...

journal_title：Plant physiology

authors： Eisenhut M,Kahlon S,Hasse D,Ewald R,Lieman-Hurwitz J,Ogawa T,Ruth W,Bauwe H,Kaplan A,Hagemann M

更新日期：2006-09-01 00:00:00

abstract：:Increasing grain sink strength by improving assimilate uptake capacity could be a promising approach toward getting higher yield. The barley (Hordeum vulgare) sucrose transporter HvSUT1 (SUT) was expressed under control of the endosperm-specific Hordein B1 promoter (HO). Compared with the wild type, transgenic HOSUT g...

journal_title：Plant physiology

authors： Weichert N,Saalbach I,Weichert H,Kohl S,Erban A,Kopka J,Hause B,Varshney A,Sreenivasulu N,Strickert M,Kumlehn J,Weschke W,Weber H

更新日期：2010-02-01 00:00:00

abstract：:In Chlamydomonas reinhardtii, a light-induced oxidative stress shifts the glutathione pool toward its oxidized form, resulting in a translational arrest of the large subunit (LSU) of Rubisco. We show here that the translational arrest of LSU is tightly coordinated with cessation of Rubisco assembly, and both processes...

journal_title：Plant physiology

authors： Cohen I,Knopf JA,Irihimovitch V,Shapira M

更新日期：2005-02-01 00:00:00

abstract：:The turnover of dhurrin in green seedlings of Sorghum bicolor (Linn) Moench var Redland x Greenleaf, Sudan 70 has been investigated using glyphosate and pulse-labeling studies with (14)C-tyrosine and [(14)C]shikimic acid. The rate of dhurrin breakdown was 4.8 nanomoles per hour in the shoot and 1.4 nanomoles per hour ...

journal_title：Plant physiology

authors： Adewusi SR

更新日期：1990-11-01 00:00:00

abstract：:The metabolism of d- and l-methionine by immature cotyledons of soybean (Glycine max, L. cv Elf) grown in culture has been investigated using solid-state (13)C and (15)N nuclear magnetic resonance. d-Methionine is taken up by the cotyledons and converted to an amide, most likely by N-malonylation. About 16% of the l-m...

journal_title：Plant physiology

authors： Coker GT,Garbow JR,Schaefer J

更新日期：1987-03-01 00:00:00