Enzymatic synthesis of isoprene from dimethylallyl diphosphate in aspen leaf extracts.

Abstract:

:Aspen (Populus tremuloides Michx.) leaf extracts contain a newly discovered enzyme activity that catalyzes the magnesium ion-dependent elimination of diphosphate from dimethylallyl diphosphate with rearrangement to form isoprene (2-methyl, 1-3-butadiene). This isoprene synthase activity has been partially purified. The nonenzymatic reaction of dimethylallyl diphosphate to isoprene, known to be acid catalyzed, may be insignificant at physiological pH. In contrast, the enzymatic reaction may be responsible for the majority of light-dependent isoprene production by isoprene-emitting plants.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Silver GM,Fall R

doi

10.1104/pp.97.4.1588

subject

Has Abstract

pub_date

1991-12-01 00:00:00

pages

1588-91

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

97

pub_type

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