RNA interference-mediated change in protein body morphology and seed opacity through loss of different zein proteins.

Abstract:

:Opaque or nonvitreous phenotypes relate to the seed architecture of maize (Zea mays) and are linked to loci that control the accumulation and proper deposition of storage proteins, called zeins, into specialized organelles in the endosperm, called protein bodies. However, in the absence of null mutants of each type of zein (i.e. alpha, beta, gamma, and delta), the molecular contribution of these proteins to seed architecture remains unclear. Here, a double null mutant for the delta-zeins, the 22-kD alpha-zein, the beta-zein, and the gamma-zein RNA interference (RNAi; designated as z1CRNAi, betaRNAi, and gammaRNAi, respectively) and their combinations have been examined. While the delta-zein double null mutant had negligible effects on protein body formation, the betaRNAi and gammaRNAi alone only cause slight changes. Substantial loss of the 22-kD alpha-zeins by z1CRNAi resulted in protein body budding structures, indicating that a sufficient amount of the 22-kD zeins is necessary for maintenance of a normal protein body shape. Among different mutant combinations, only the combined betaRNAi and gammaRNAi resulted in drastic morphological changes, while other combinations did not. Overexpression of alpha-kafirins, the homologues of the maize 22-kD alpha-zeins in sorghum (Sorghum bicolor), in the beta/gammaRNAi mutant failed to offset the morphological alterations, indicating that beta- and gamma-zeins have redundant and unique functions in the stabilization of protein bodies. Indeed, opacity of the beta/gammaRNAi mutant was caused by incomplete embedding of the starch granules rather than by reducing the vitreous zone.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Wu Y,Messing J

doi

10.1104/pp.110.154690

subject

Has Abstract

pub_date

2010-05-01 00:00:00

pages

337-47

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.110.154690

journal_volume

153

pub_type

杂志文章
  • Acclimation to High CO2 Requires the ω Subunit of the RNA Polymerase in Synechocystis.

    abstract::Inactivation of the nonessential ω-subunit of the RNA polymerase core in the ΔrpoZ strain of the model cyanobacterium Synechocystis sp. PCC 6803 leads to a unique high-CO2-sensitive phenotype. Supplementing air in the growth chamber with 30 mL L-1 (3%) CO2 accelerated the growth rate of the control strain (CS) 4-fold,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01953

    authors: Kurkela J,Hakkila K,Antal T,Tyystjärvi T

    更新日期:2017-05-01 00:00:00

  • Developmental and environmental regulation of the Nicotiana plumbaginifolia cytosolic Cu/Zn-superoxide dismutase promoter in transgenic tobacco.

    abstract::Superoxide dismutases (SODs) play a key role in the cellular defense against reactive oxygen species. To study the transcriptional regulation at the cellular level, the promoter of the Nicotiana plumbaginifolia cytosolic gene encoding Cu/ZnSOD (SODCc) was fused to the beta-glucuronidase (GUS) reporter gene (gusA) and ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.3.873

    authors: Hérouart D,Van Montagu M,Inzé D

    更新日期:1994-03-01 00:00:00

  • Characterization of a new antifungal chitin-binding peptide from sugar beet leaves.

    abstract::The intercellular washing fluid (IWF) from leaves of sugar beet (Beta vulgaris L.) contains a number of proteins exhibiting in vitro antifungal activity against the devastating leaf pathogen Cercospora beticola (Sacc.). Among these, a potent antifungal peptide, designated IWF4, was identified. The 30-amino-acid residu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.1.83

    authors: Nielsen KK,Nielsen JE,Madrid SM,Mikkelsen JD

    更新日期:1997-01-01 00:00:00

  • A wound- and methyl jasmonate-inducible transcript coding for a myrosinase-associated protein with similarities to an early nodulin.

    abstract::Myrosinase is regarded as a defense-related enzyme in the Brassicaceae and is capable of hydrolyzing glucosinolates into various compounds, some of which are toxic. Several myrosinase isoenzymes exist, and some of them have been found in association with nonmyrosinase proteins. One of these associated proteins, myrosi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.2.483

    authors: Taipalensuu J,Falk A,Rask L

    更新日期:1996-02-01 00:00:00

  • Effect of temperature on photosynthesis and growth in marine Synechococcus spp.

    abstract::In this study, we develop a mechanistic understanding of how temperature affects growth and photosynthesis in 10 geographically and physiologically diverse strains of Synechococcus spp. We found that Synechococcus spp. are able to regulate photochemistry over a range of temperatures by using state transitions and alte...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.221937

    authors: Mackey KR,Paytan A,Caldeira K,Grossman AR,Moran D,McIlvin M,Saito MA

    更新日期:2013-10-01 00:00:00

  • StGCPRP, a potato gene strongly expressed in stomatal guard cells, defines a novel type of repetitive proline-rich proteins.

    abstract::Guard cells represent a highly differentiated cell type within the epidermis of plant leaves and stems. They respond to many endogenous and environmental signals and thereby modify the size of the stomatal pore they surround. We identified a novel gene that is highly expressed in guard cells of potato (Solanum tuberos...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.3.677

    authors: Menke U,Renault N,Mueller-Roeber B

    更新日期:2000-03-01 00:00:00

  • Effects of freezing and hardening on the sulfhydryl groups of protein fractions from cabbage leaves.

    abstract::Disc electrophoresis was used to separate water soluble proteins from hardy, non-hardy, and frost killed cabbage (Brassica oleracea var. capitata) leaves. Amidoschwarz staining failed to reveal any new bands as a result of hardening although the relative amounts of proteins in individual bands changed. Sulfhydryl grou...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.2.168

    authors: Morton WM

    更新日期:1969-02-01 00:00:00

  • The DEK1 Calpain Linker Functions in Three-Dimensional Body Patterning in Physcomitrella patens.

    abstract::The DEFECTIVE KERNEL1 (DEK1) calpain is a conserved 240-kD key regulator of three-dimensional body patterning in land plants acting via mitotic cell plane positioning. The activity of the cytosolic C-terminal calpain protease is regulated by the membrane-anchored DEK1 MEM, which is connected to the calpain via the 600...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00925

    authors: Johansen W,Ako AE,Demko V,Perroud PF,Rensing SA,Mekhlif AK,Olsen OA

    更新日期:2016-10-01 00:00:00

  • Light-regulated and cell-specific expression of tomato rbcS-gusA and rice rbcS-gusA fusion genes in transgenic rice.

    abstract::A previously isolated rice (Oryza sativa) rbcS gene was further characterized. This analysis revealed specific sequences in the 5' regulatory region of the rice rbcS gene that are conserved in rbcS genes of other monocotyledonous species. In transgenic rice plants, we examined the expression of the beta-glucuronidase ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.3.991

    authors: Kyozuka J,McElroy D,Hayakawa T,Xie Y,Wu R,Shimamoto K

    更新日期:1993-07-01 00:00:00

  • Regulation of ribulose-1,5-bisphosphate carboxylase expression in second leaves of maize seedlings from low and high yield populations.

    abstract::Ribulose-1,5-bisphosphate carboxylase oxygenase (EC 4.1.1.39) (Rubisco) activity, Rubisco-protein, and Rubisco large and small subunit gene (rbcL and rbcS) transcripts were measured at seven stages of development in the second leaf of maize (Zea mays L.) seedlings belonging to low and high yield populations. During th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.541

    authors: Loza-Tavera H,Martínez-Barajas E,Sánchez-de-Jiménez E

    更新日期:1990-06-01 00:00:00

  • Selection of Nicotiana tabacum Haploids of High Photosynthetic Efficiency.

    abstract::Anthers of Nicotiana tabacum cultivar Wisconsin 38 were treated with the mutagen ethyl methane sulfonate and cultured to obtain populations of haploid plants of high genetic variability. The selection of these populations by their photosynthetic efficiency was carried out in a hydroponic culture chamber with a CO(2) a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.2.505

    authors: Medrano H,Primo-Millo E

    更新日期:1985-10-01 00:00:00

  • Extinction coefficients of chlorophyll a and B in n,n-dimethylformamide and 80% acetone.

    abstract::We found inconsistencies in the commonly used data for chlorophyll analysis in 80% acetone. Recently developed extinction coefficients for chlorophyll b in N,N-dimethylformamide (DMF) based on values from 80% acetone are low as a result of these inconsistencies. We determined extinction coefficients of chlorophyll a (...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.2.483

    authors: Inskeep WP,Bloom PR

    更新日期:1985-02-01 00:00:00

  • Analysis of the small GTPase gene superfamily of Arabidopsis.

    abstract::Small GTP-binding proteins regulate diverse processes in eukaryotic cells such as signal transduction, cell proliferation, cytoskeletal organization, and intracellular membrane trafficking. These proteins function as molecular switches that cycle between "active" and "inactive" states, and this cycle is linked to the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.013052

    authors: Vernoud V,Horton AC,Yang Z,Nielsen E

    更新日期:2003-03-01 00:00:00

  • Phytochrome-mediated Induction of Phenylalanine Ammonia-Lyase in Mustard Seedlings: A Contribution to Eliminate Some Misconceptions.

    abstract::The present report shows that the effect of red and far red light on the synthesis of phenylalanine ammonia-lyase can be ascribed to the action of phytochrome Pfr. This is true not only for short term irradiation but also for the action of continuous far red light. In the latter case, the model worked out by Hartmann ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.1.8

    authors: Schopfer P,Mohr H

    更新日期:1972-01-01 00:00:00

  • Preparation of Membrane Vesicles Enriched in ATP-Dependent Proton Transport from Suspension Cultures of Tomato Cells.

    abstract::Membranes enriched in ATP-dependent proton transport were prepared from suspension cultures of tomato cells (Lycopersicon esculentum Mill cv VF36). Suspension cultures were a source of large quantities of membranes from rapidly growing, undifferentiated cells. Proton transport activity was assayed as quench of acridin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.1.69

    authors: Dupont FM,Zabala Mde G

    更新日期:1985-01-01 00:00:00

  • Phosphate Treatment Strongly Inhibits New Arbuscule Development But Not the Maintenance of Arbuscule in Mycorrhizal Rice Roots.

    abstract::Phosphorus (P) is a crucial nutrient for plant growth, but its availability to roots is limited in soil. Arbuscular mycorrhizal (AM) symbiosis is a promising strategy for improving plant P acquisition. However, P fertilizer reduces fungal colonization (P inhibition) and compromises mycorrhizal P uptake, warranting stu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00127

    authors: Kobae Y,Ohmori Y,Saito C,Yano K,Ohtomo R,Fujiwara T

    更新日期:2016-05-01 00:00:00

  • Deep Supercooling in Most Tissues of Wintering Sasa senanensis and Its Mechanism in Leaf Blade Tissues.

    abstract::Cold hardiness of leaf blades, leaf sheaths, culms, rhizomes, and leaf buds in wintering Sasa senanensis (Fr. et Sav.) Rehder, a dwarf bamboo, was studied paying special attention to the types of resistance mechanisms which were determined with differential thermal analysis. Coincidence of LT(25) (lethal temperature a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.1.196

    authors: Ishikawa M

    更新日期:1984-05-01 00:00:00

  • Interaction of water supply and N in wheat.

    abstract::The purpose of this study was to investigate effects of N nutrition and water stress on stomatal behavior and CO(2) exchange rate in wheat (Triticum aestivum L. cv Olaf). Wheat plants were grown hydroponically with high (100 milligrams per liter) and low (10 milligrams per liter) N. When plants were 38 days old, a 24-...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.1.112

    authors: Morgan JA

    更新日期:1984-09-01 00:00:00

  • Effect of substrate salinity on the ability for protein synthesis in pea roots.

    abstract::THE EFFECT OF SALINITY ON INCORPORATION OF AMINO ACIDS INTO ROOT TIP PROTEIN IS APPARENTLY OF DUAL NATURE: in presence of salts the uptake is depressed and the normal metabolic pathways are disturbed. If the roots were grown at high salt concentration, uptake and incorporation are affected even if they are carried out...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.7.1115

    authors: Kahane I,Poljakoff-Mayber A

    更新日期:1968-07-01 00:00:00

  • A comprehensive analysis of the NADP-malic enzyme gene family of Arabidopsis.

    abstract::The Arabidopsis (Arabidopsis thaliana) genome contains four genes encoding putative NADP-malic enzymes (MEs; AtNADP-ME1-ME4). NADP-ME4 is localized to plastids, whereas the other three isoforms do not possess any predicted organellar targeting sequence and are therefore expected to be cytosolic. The plant NADP-MEs can...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.065953

    authors: Wheeler MC,Tronconi MA,Drincovich MF,Andreo CS,Flügge UI,Maurino VG

    更新日期:2005-09-01 00:00:00

  • Chemical and Biophysical Changes in the Plasma Membrane during Cold Acclimation of Mulberry Bark Cells (Morus bombycis Koidz. cv Goroji).

    abstract::The lipid and protein composition of the plasma membrane isolated from mulberry (Morus bombycis Koidz.) bark cells was analyzed throughout the cold acclimation period under natural and controlled environment conditions. There was a significant increase in phospholipids and unsaturation of their fatty acids during cold...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.1.257

    authors: Yoshida S

    更新日期:1984-09-01 00:00:00

  • Evolutionary expansion, gene structure, and expression of the rice wall-associated kinase gene family.

    abstract::The wall-associated kinase (WAK) gene family, one of the receptor-like kinase (RLK) gene families in plants, plays important roles in cell expansion, pathogen resistance, and heavy-metal stress tolerance in Arabidopsis (Arabidopsis thaliana). Through a reiterative database search and manual reannotation, we identified...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.069005

    authors: Zhang S,Chen C,Li L,Meng L,Singh J,Jiang N,Deng XW,He ZH,Lemaux PG

    更新日期:2005-11-01 00:00:00

  • Molecular and functional analyses support a role of Ornithine-{delta}-aminotransferase in the provision of glutamate for glutamine biosynthesis during pine germination.

    abstract::We report the molecular characterization and functional analysis of a gene (PsdeltaOAT) from Scots pine (Pinus sylvestris) encoding Orn-delta-aminotransferase (delta-OAT; EC 2.6.1.13), an enzyme of arginine metabolism. The deduced amino acid sequence contains a putative N-terminal signal peptide for mitochondrial targ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.122853

    authors: Cañas RA,Villalobos DP,Díaz-Moreno SM,Cánovas FM,Cantón FR

    更新日期:2008-09-01 00:00:00

  • Stop-and-go movements of plant Golgi stacks are mediated by the acto-myosin system.

    abstract::The Golgi apparatus in plant cells consists of a large number of independent Golgi stack/trans-Golgi network/Golgi matrix units that appear to be randomly distributed throughout the cytoplasm. To study the dynamic behavior of these Golgi units in living plant cells, we have cloned a cDNA from soybean (Glycine max), Gm...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.121.4.1127

    authors: Nebenführ A,Gallagher LA,Dunahay TG,Frohlick JA,Mazurkiewicz AM,Meehl JB,Staehelin LA

    更新日期:1999-12-01 00:00:00

  • Role of the putative osmosensor Arabidopsis histidine kinase1 in dehydration avoidance and low-water-potential response.

    abstract::The molecular basis of plant osmosensing remains unknown. Arabidopsis (Arabidopsis thaliana) Histidine Kinase1 (AHK1) can complement the osmosensitivity of yeast (Saccharomyces cerevisiae) osmosensor mutants lacking Synthetic Lethal of N-end rule1 and SH3-containing Osmosensor and has been proposed to act as a plant o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.209791

    authors: Kumar MN,Jane WN,Verslues PE

    更新日期:2013-02-01 00:00:00

  • GbPDF1 is involved in cotton fiber initiation via the core cis-element HDZIP2ATATHB2.

    abstract::Cotton (Gossypium spp.) fiber cells are seed trichomes derived from the epidermal layer of the cotton seed coat. The molecular components responsible for regulating fiber cell differentiation have not been fully elucidated. A cotton PROTODERMAL FACTOR1 gene (GbPDF1) was found to be expressed preferentially during fibe...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.186742

    authors: Deng F,Tu L,Tan J,Li Y,Nie Y,Zhang X

    更新日期:2012-02-01 00:00:00

  • Modification of cytokinins by cauliflower microsomal enzymes.

    abstract::N(6)-(Delta(2)-isopentenyl)Adenine and N(6)-(Delta(2)-isopentenyl)adenosine were hydroxylated, respectively, to 6-(4-hydroxy-3-methyl-trans-2-butenylamino)purine and 6-(4-hydroxy-3-methyl-trans-2-butenylamino) -9-beta-ribofuranosylpurine in the presence of NADPH and the microsomal fraction from cauliflowers (Brassica ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.2.442

    authors: Chen CM,Leisner SM

    更新日期:1984-06-01 00:00:00

  • Circadian rhythmicity in excised samanea pulvini: I. Sucrose-white light interactions.

    abstract::The rhythmic movement of excised Samanea saman pulvini incubated in H(2)O or 50 mm sucrose was monitored during extended periods of white light (cool white fluorescent, 2,000 ft-c), darkness, or alternating white light (16 hr) and darkness (8 hr). In continuous white light, the rhythm damps at an intermediate angle af...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.3.417

    authors: Simon E,Satter RL,Galston AW

    更新日期:1976-09-01 00:00:00

  • Dormancy, Turion Formation, and Germination by Different Clones of Spirodela polyrrhiza.

    abstract::Some clones of Spirodela polyrrhiza form dormant bodies called turions which require several weeks of chilling treatment before they proceed to renew growth and develop into vegetative fronds. The individual fronds of Spirodela are less than 5 mm long and can be grown aseptically in liquid culture. Turion formation an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.11.1866

    authors: Perry TO

    更新日期:1968-11-01 00:00:00

  • Structure of Plant Cell Walls : XVIII. An Analysis of the Extracellular Polysaccharides of Suspension-Cultured Sycamore Cells.

    abstract::The water-soluble polysaccharides (SEPS) secreted into the medium by suspension-cultured sycamore cells were examined to determine whether the polysaccharides were the same as those present in the walls of sycamore cells. The SEPS were made more amenable to fractionation by treatment with a highly purified alpha-1,4-e...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.4.1012

    authors: Stevenson TT,McNeil M,Darvill AG,Albersheim P

    更新日期:1986-04-01 00:00:00