Abstract:
:Inactivation of the nonessential ω-subunit of the RNA polymerase core in the ΔrpoZ strain of the model cyanobacterium Synechocystis sp. PCC 6803 leads to a unique high-CO2-sensitive phenotype. Supplementing air in the growth chamber with 30 mL L-1 (3%) CO2 accelerated the growth rate of the control strain (CS) 4-fold, whereas ΔrpoZ did not grow faster than under ambient air. The slow growth of ΔrpoZ during the first days in high CO2 was due to the inability of the mutant cells to adjust photosynthesis to high CO2 The light-saturated photosynthetic activity of ΔrpoZ in high CO2 was only half of that measured in CS, Rubisco content was one-third lower, and cells of ΔrpoZ were not able to increase light-harvesting phycobilisome antenna like CS upon high-CO2 treatment. In addition, altered structural and functional organization of photosystem I and photosystem II were detected in the ΔrpoZ strain compared with CS when cells were grown in high CO2 but not in ambient air. Moreover, respiration of ΔrpoZ did not acclimate to high CO2 Unlike the photosynthetic complexes, the RNA polymerase complex and ribosomes were produced in high CO2 similarly as in CS Our results indicate that the deletion of the ω-subunit specifically affects photosynthesis and respiration, but transcription and translation remain active. Thus, the specific effect of the ω-subunit on photosynthesis but not on all household processes suggests that the ω-subunit might have a regulatory function in cyanobacteria.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Kurkela J,Hakkila K,Antal T,Tyystjärvi Tdoi
10.1104/pp.16.01953subject
Has Abstractpub_date
2017-05-01 00:00:00pages
172-184issue
1eissn
0032-0889issn
1532-2548pii
pp.16.01953journal_volume
174pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The effects of high light stress on chloroplast ultrastructure and protein and mRNA composition were investigated in carotenoid-deficient peas (Pisum sativum, L.). In low light, the thylakoid membrane polypeptide pattern was altered, with several prominent chlorophyll-binding proteins present in diminished amounts. Th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.4.1663
更新日期:1990-12-01 00:00:00
abstract::The cDNA for 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase of Arabidopsis encodes a polypeptide with an amino-terminal signal sequence for plastid import. A cDNA fragment encoding the processed form of the enzyme was expressed in Escherichia coli. The resulting protein was purified to electrophoretic homogeneit...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.002626
更新日期:2002-08-01 00:00:00
abstract::Field-grown tuber-bearing potatoes were screened for frost tolerance in a late stage of development. Three different clones of Solanum tuberosum L. and two interspecific crosses between clones of S. tuberosum and the wild potato species S. demissum Lindl. were studied. Two different methods were used. (a) Temperature-...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.5.1299
更新日期:1982-11-01 00:00:00
abstract::Expression of a beta-amylase gene of Arabidopsis thaliana (AT beta-Amy) is regulated by sugars. We identified a mutant, hba1, in which the level of expression of AT beta-Amy in leaves of plants that had been grown in a medium with 2% sucrose was significantly higher than that in wild-type plants. Higher that wild-type...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.2.575
更新日期:1997-06-01 00:00:00
abstract::The Arabidopsis genome contains 14 genes encoding the serine protease DegP. Products of four of these genes are located in the chloroplast: three in the thylakoid lumen and one on the stromal side of the membrane. We expressed the gene encoding DegP1 as a His-tagged fusion protein in Escherichia coli, purified the pro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.007922
更新日期:2002-10-01 00:00:00
abstract::Gas exchange and plant luminescence (delayed light emission) of a single red kidney bean leaf undergoing synchronous oscillations in gas exchange were recorded and analyzed. Introduction of 1.1 microliter per liter SO(2) during these oscillations produced increases in plant luminescence that, when averaged over a port...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.4.904
更新日期:1985-08-01 00:00:00
abstract::Membrane transporters play a central role in many cellular processes that rely on the movement of ions and organic molecules between the environment and the cell, and between cellular compartments. Transporters have been well characterized in plants and green algae, but little is known about transporters or their evol...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.193896
更新日期:2012-04-01 00:00:00
abstract::We present four examples of attenuation of the transformed phenotype caused by the root-inducing, left-hand, transferred DNA from Agrobacterium rhizogenes in tobacco (Nicotiana tabacum). The first was associated with a genetic variable (homozygosity for the T-DNA), and the second was induced at the physiological level...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.1.259
更新日期:1996-05-01 00:00:00
abstract::The mitochondrial flavoenzyme l-galactono-gamma-lactone dehydrogenase (GALDH) catalyzes the ultimate step of vitamin C biosynthesis in plants. We found that recombinant GALDH from Arabidopsis (Arabidopsis thaliana) is inactivated by hydrogen peroxide due to selective oxidation of cysteine (Cys)-340, located in the cap...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.136929
更新日期:2009-06-01 00:00:00
abstract::A Mg(2+)-dependent, cation-stimulated ATPase was associated with plasma membranes isolated from corn leaf mesophyll protoplasts. Potassium was the preferred monovalent cation for stimulating the ATPase above the Mg(2+)-activated level. The enzyme was substrate-specific for ATP, was inhibited by N,N'-dicyclohexylcarbod...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.3.521
更新日期:1981-09-01 00:00:00
abstract::Samples of irradiated and control Avena sativa shoot tissue were homogenized together to determine whether, during homogenization phytochrome from irradiated tissue can bind to the particulate material simultaneously extracted from the control tissue. The level of phytochrome pelletability for such mixed tissue homoge...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.5.908
更新日期:1980-11-01 00:00:00
abstract::Subcellular organelles from castor bean (Ricinus communis) endosperm were isolated on discontinuous sucrose gradients from germinating seeds which were 1 to 7 days postimbibition. Marker enzyme activities of the organelles were measured (fumarase, catalase, and triose phosphate isomerase) and the homogeneity of the or...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.2.314
更新日期:1980-02-01 00:00:00
abstract::The Aurora B kinase, encoded by the AURORA 3 (AUR3) gene in Arabidopsis (Arabidopsis thaliana), is a key regulator of cell division in all eukaryotes. Aurora B has at least two central functions during cell division; it is essential for the correct, i.e. balanced, segregation of chromosomes in mitosis and meiosis by c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00344
更新日期:2020-08-01 00:00:00
abstract::Coupling factor particles are associated with membranes of maize etioplasts (Lockshin et al., 1971. Biochim. Biophys. Acta 226: 366-382). In addition, several, but not all, of the polypeptides found in the photosynthetic lamellae of maize chloroplasts are present in etioplasts.During the photoregulated maturation of c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.5.491
更新日期:1973-11-01 00:00:00
abstract::Transgenic Medicago truncatula plants were produced harboring chimeric gene constructs of the glutamine synthetase (GS) cDNA clones (MtGS1a or MtGS1b) fused in sense or antisense orientation to the nodule-specific leghemoglobin promoter Mtlb1. A series of transgenic plants were obtained showing a 2- to 4-fold alterati...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.017830
更新日期:2003-09-01 00:00:00
abstract::We investigated the role of gibberellins (GAs) in the effect of pat-2, a recessive mutation that induces facultative parthenocarpic fruit development in tomato (Lycopersicon esculentum Mill.) using near-isogenic lines with two different genetic backgrounds. Unpollinated wild-type Madrigal (MA/wt) and Cuarenteno (CU/wt...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.2.471
更新日期:2000-02-01 00:00:00
abstract::A novel alpha-L-fucosyltransferase capable of transferring L-fucose (L-Fuc) from GDP-L-Fuc to the O-2 of alpha-L-arabinofuranosyl residue (GDP-L-Fuc:alpha-L-arabinofuranoside 2-alpha-L-fucosyltransferase) has been found in the microsomal fraction of primary roots from 6-d-old radish (Raphanus sativus L.) seedlings. En...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.2.665
更新日期:1996-02-01 00:00:00
abstract::Intact amyloplasts from potato (Solanum tuberosum L.) were used to study starch biosynthesis and phosphorylation. Assessed by the degree of intactness and by the level of cytosolic and vacuolar contamination, the best preparations were selected by searching for amyloplasts containing small starch grains. The isolated,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.2.455
更新日期:1999-02-01 00:00:00
abstract::Cyclin-dependent kinases (CDKs) form a conserved superfamily of eukaryotic serine-threonine protein kinases whose activity requires the binding of a cyclin protein. CDKs are involved in many aspects of cell biology and notably in the regulation of the cell cycle. Three cDNAs encoding a C-type CDK, and a member of each...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.4.1403
更新日期:2001-08-01 00:00:00
abstract::The origin of the ethylene carbon skeleton in Penicillium digitatum appears to be intimately associated with the Krebs cycle acids, particularly the middle carbon atoms of dicarboxylic acids. Among the other compounds studied, certain carbon atoms of beta-alanine, propionic acid, and methionine can be incorporated int...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.12.1959
更新日期:1968-12-01 00:00:00
abstract::Changes in cytoplasmic Ca2+ concentration ([Ca2+]i) have been proposed to be involved in signal transduction pathways in response to a number of stimuli, including gravity and touch. The current hypothesis proposes that the development of gravitropic bending is correlated with a redistribution of [Ca2+]i in gravistimu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.3.789
更新日期:1997-07-01 00:00:00
abstract::Seed dormancy is a common phase of the plant life cycle, and several parts of the seed can contribute to dormancy. Whole seeds, seeds lacking the testa, embryos, and isolated aleurone layers of Arabidopsis (Arabidopsis thaliana) were used in experiments designed to identify components of the Arabidopsis seed that cont...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.093435
更新日期:2007-03-01 00:00:00
abstract::Movement of [(14)C]kinetin and [(14)C]gibberellic acid was examined in cotton (Gossypium hirsutum L.) cotyledonary petiole sections independent of label uptake or exit from the tissue. Sections 20 millimeters in length were taken from well watered, stressed, and poststressed plants. Transport capacity was determined u...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.1.152
更新日期:1979-01-01 00:00:00
abstract::Chenopods synthesize betaine by a two-step oxidation of choline: choline --> betaine aldehyde --> betaine. The pathway is chloroplastic; the first step has been shown in isolated spinach (Spinacia oleracea L.) chloroplasts to be O(2)- and light-dependent, the role of light being to provide reducing power (P Weigel, EA...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.3.695
更新日期:1988-11-01 00:00:00
abstract::Since peroxidase and manganese have been implicated in both auxin destruction and ethylene production, the effect of auxins and high tissue levels of manganese on the peroxidative indoleacetic acid oxidase system and the internal level of ethylene was determined in cotton (Gossypium hirsutum L. cv. Watson GL-7). The h...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.4.555
更新日期:1972-04-01 00:00:00
abstract::A technique of centrifuging pea epicotyl sections which extracts water-soluble cell wall polysaccharides with less than 1.5% cytoplasmic contamination as revealed by malate dehydrogenase activity determinations was developed. Tests for protein, hexose, pentose, and malate dehydrogenase indicate that significant damage...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.2.321
更新日期:1980-08-01 00:00:00
abstract::Reduction of Cu2+ by plasma membrane vesicles isolated from tomato (Lycopersicon esculentum Mill.) roots was investigated. Plants were grown in hydroponic culture with complete nutrition for 4 weeks or were deprived of Fe for the last 7 d. Plasma membrane vesicles were prepared by aqueous two-phase partitioning. Reduc...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.3.1093
更新日期:1995-07-01 00:00:00
abstract::Mature maize leaves have defined cell types along the proximal distal and medial lateral axes. The patterning events that establish these axes take place early in leaf initiation. We have identified a new dominant mutation, Wavy auricle in blade1 (Wab1), which affects patterning in both axes in a dose-dependent manner...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.036707
更新日期:2004-05-01 00:00:00
abstract::Alternative splicing (AS) can add significantly to genome complexity. Plants are thought to exhibit less AS than animals. An algorithm, based on expressed sequence tag (EST) pairs gapped alignment, was developed that takes advantage of the relatively small intron and exon size in plants and directly compares pairs of ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.098640
更新日期:2007-07-01 00:00:00
abstract::The rate of malic acid consumption in the Crassulacean acid metabolism (CAM) plant Kalanchoë daigremontiana Hamet et Perrier was found to be more rapid than the rate of photosynthetic oxygen evolution under all levels of irradiation by white light. This accounts for the accumulation of carbon dioxide in CAM tissues in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.3.568
更新日期:1982-03-01 00:00:00