Attenuation of the Phenotype Caused by the Root-Inducing, Left-Hand, Transferred DNA and Its rolA Gene (Correlations with Changes in Polyamine Metabolism and DNA Methylation).

Abstract:

:We present four examples of attenuation of the transformed phenotype caused by the root-inducing, left-hand, transferred DNA from Agrobacterium rhizogenes in tobacco (Nicotiana tabacum). The first was associated with a genetic variable (homozygosity for the T-DNA), and the second was induced at the physiological level by putrescine and tyramine, suggesting that the transformed phenotype depends on defective polyamine metabolism. Physiological attenuation is further illustrated in the third example, in which the inhibition of flowering caused by P35S-rolA, a gene from the root-inducing, left-hand, transferred DNA driven by a strong viral promoter, was attenuated by grafting the transformed shoot onto non-transformed rootstock that had been induced to flower. Infertility in the resulting flowers was corrected by a mixture of putrescine and tyramine, indicating that P35S-rolA inhibited flowering through interference with polyamine conjugation and that tyramine was essential to fertility. A fourth example of attenuation of the transformed phenotype occurred in lateral branches of plants expressing rolA under the control of its native promoter. In these branches, reduction in the accumulation of rolA transcripts was correlated with the methylation of a site 3[prime] to the rolA coding sequence; thus, the transformed plant seems capable of recognizing and repressing a gene that interferes with flowering.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Martin-Tanguy J,Sun LY,Burtin D,Vernoy R,Rossin N,Tepfer D

doi

10.1104/pp.111.1.259

subject

Has Abstract

pub_date

1996-05-01 00:00:00

pages

259-267

issue

1

eissn

0032-0889

issn

1532-2548

pii

111/1/259

journal_volume

111

pub_type

杂志文章
  • The LPB1 gene is important for acclimation of Chlamydomonas reinhardtii to phosphorus and sulfur deprivation.

    abstract::Organisms exhibit a diverse set of responses when exposed to low-phosphate conditions. Some of these responses are specific for phosphorus limitation, including responses that enable cells to efficiently scavenge phosphate from internal and external stores via the production of high-affinity phosphate transporters and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.059550

    authors: Chang CW,Moseley JL,Wykoff D,Grossman AR

    更新日期:2005-05-01 00:00:00

  • Effects of CO(2) and O(2) on Photosynthesis and Growth of Autotrophic Tobacco Callus.

    abstract::Mean inhibition of net photosynthesis in autotrophic tobacco callus by 21 and 40% O(2) was 30 and 47%, respectively, similar to intact leaves. Increasing CO(2) concentrations (500-2000 microliters per liter) produced a steady decline in percent inhibition at both O(2) concentrations, indicating that O(2) inhibition re...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.4.1055

    authors: McHale NA,Zelitch I,Peterson RB

    更新日期:1987-08-01 00:00:00

  • Influence of phenolic acids on ion uptake: I. Inhibition of phosphate uptake.

    abstract::The influence of naturally occurring phenolic acids on phosphate uptake by barley (Hordeum vulgare L. cv. Karlsberg) roots was examined using (32)P-labeled phosphate. Without exception, all compounds tested, namely, benzoic, 2-hydroxybenzoic, 4-hydroxybenzoic, 3,4-dihydroxybenzoic, 3,4,5-trihydroxybenzoic, 4-hydroxy-3...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.6.1037

    authors: Glass AD

    更新日期:1973-06-01 00:00:00

  • The Lipid Flippases ALA4 and ALA5 Play Critical Roles in Cell Expansion and Plant Growth.

    abstract::Aminophospholipid ATPases (ALAs) are lipid flippases involved in transporting specific lipids across membrane bilayers. Arabidopsis (Arabidopsis thaliana) contains 12 ALAs in five phylogenetic clusters, including four in cluster 3 (ALA4-ALA7). ALA4/5 and ALA6/7, are expressed primarily in vegetative tissues and pollen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01332

    authors: Davis JA,Pares RB,Bernstein T,McDowell SC,Brown E,Stubrich J,Rosenberg A,Cahoon EB,Cahoon RE,Poulsen LR,Palmgren M,López-Marqués RL,Harper JF

    更新日期:2020-04-01 00:00:00

  • Bound auxin formation in growing stems.

    abstract::The term "bound auxin" is herein used to describe auxin conjugates insoluble in organic solvents which dissolve indoleacetic acid (IAA) and its derivatives, but hydrolyzable by NaOH to release IAA. Bound auxin from pea stems was fractionated into water-soluble, water-insoluble/NaOH-hydrolyzable, and insoluble componen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.2.197

    authors: Davies PJ

    更新日期:1976-02-01 00:00:00

  • Blue-Light Regulation of Epicotyl Elongation in Pisum sativum.

    abstract::Blue light is known to induce suppression of stem elongation. To avoid the complication of blue-light-induced transformation of phytochrome we have adapted the procedure of measuring blue-light-induced suppression of stem elongation in Pisum sativum L. var Alaska grown under continuous red light. The resulting fluence...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.2.544

    authors: Warpeha KM,Kaufman LS

    更新日期:1989-02-01 00:00:00

  • Pentose phosphate metabolism of potato tuber discs as influenced by prior storage temperature.

    abstract::Tubers of Solanum tuberosum L. var. Russet Burbank, Kennebec, and Targhee were stored at 95% relative humidity and at 1.7, 4.4, 5.8, 7.2 or 10 C. Rates of O(2) consumption were measured periodically by removing individual tubers from storage, excising discs of pith parenchyma tissue, and incubating the discs in a Gils...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.2.252

    authors: Dwelle RB,Stallknecht GF

    更新日期:1978-02-01 00:00:00

  • A Cobalt Requirement for Symbiotic Growth of Azolla filiculoides in the Absence of Combined Nitrogen.

    abstract::Cobalt was shown to be essential for the symbiotic growth of Azolla filiculoides and Anabacna azollac in the absence of fixed nitrogen. Addition of 0.01 mug/liter cobalt resulted in large increases in yield, chlorophyll content and nitrogen fixation as compared to control cultures without cobalt. Cobalt was not requir...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.5.852

    authors: Johnson GV,Mayeux PA,Evans HJ

    更新日期:1966-05-01 00:00:00

  • Effects of Air Pollutants on the Composition of Stable Carbon Isotopes, deltaC, of Leaves and Wood, and on Leaf Injury.

    abstract::Air pollutants are known to cause visible leaf injury as well as impairment of photosynthetic CO(2) fixation. Here we evaluate whether the effects on photosynthesis are large enough to cause changes in the relative composition of stable carbon isotopes, delta(13)C, of plant tissue samples, and, if so, how the changes ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.1.218

    authors: Martin B,Bytnerowicz A,Thorstenson YR

    更新日期:1988-09-01 00:00:00

  • PROTEIN PHOSHATASE 2A B'α and β Maintain Centromeric Sister Chromatid Cohesion during Meiosis in Arabidopsis.

    abstract::The correct separation of homologous chromosomes during meiosis I, and sister chromatids during meiosis II, relies on the tight control of the cohesion complex. The phosphorylation and subsequent cleavage of the meiotic recombination protein REC8 (REC8-like family protein [SYN1] in Arabidopsis [Arabidopsis thaliana]),...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.00281

    authors: Yuan G,Ahootapeh BH,Komaki S,Schnittger A,Lillo C,De Storme N,Geelen D

    更新日期:2018-09-01 00:00:00

  • Insertional mutagenesis using Tnt1 retrotransposon in potato.

    abstract::Insertional mutagenesis using transfer DNA or transposable elements, which is an important tool in functional genomics and is well established in several crops, has not been developed in potato (Solanum tuberosum). Here, we report the application of the tobacco (Nicotiana tabacum) Tnt1 retrotransposon as an insertiona...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.221903

    authors: Duangpan S,Zhang W,Wu Y,Jansky SH,Jiang J

    更新日期:2013-09-01 00:00:00

  • MEKK1 is required for flg22-induced MPK4 activation in Arabidopsis plants.

    abstract::The Arabidopsis (Arabidopsis thaliana) gene MEKK1 encodes a mitogen-activated protein kinase kinase kinase that has been implicated in the activation of the map kinases MPK3 and MPK6 in response to the flagellin elicitor peptide flg22. In this study, analysis of plants carrying T-DNA knockout alleles indicated that ME...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.091389

    authors: Suarez-Rodriguez MC,Adams-Phillips L,Liu Y,Wang H,Su SH,Jester PJ,Zhang S,Bent AF,Krysan PJ

    更新日期:2007-02-01 00:00:00

  • Messenger RNA-controlled Increase of Phenylalanine Ammonia-Lyase Activity in Parsley: Light-Independent Induction by Dilution of Cell Suspension Cultures into Water.

    abstract::A specific antiserum, raised against purified phenylalanine ammonialyase from irradiated cell suspension cultures of parsley (Petroselinum hortense Hoffm.), was used to compare the enzyme species induced either by dilution or by irradiation of the cell suspensions, to investigate the effect of dilution on the rate of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.3.440

    authors: Schröder J,Betz B,Hahlbrock K

    更新日期:1977-09-01 00:00:00

  • Requirement for the H phosphoprotein in photosystem II of Chlamydomonas reinhardtii.

    abstract::To dissect the expression of the psbB gene cluster of the Chlamydomonas reinhardtii chloroplast genome and to assess the role of the photosystem II H-phosphoprotein (PSII-H) in the biogenesis and/or stabilization of PSII, an aadA gene cassette conferring spectinomycin resistance was employed for mutagenesis. Disruptio...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.4.1359

    authors: Summer EJ,Schmid VH,Bruns BU,Schmidt GW

    更新日期:1997-04-01 00:00:00

  • Effect of Heat Shock on Plant Growth and on Lipid and beta-Glucan Syntheses.

    abstract::Primary leaves of 8-day-old Phaseolus vulgaris plants dipped in water at 46.5 to 47.5 C for 2 minutes were found to have decreased synthesis of lipid and beta-glucan compared to controls; UDP-glucose was used as substrate by cell-free preparations. As time elapsed, leaf growth, which was inhibited within the first 12 ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.1.118

    authors: Ordin L,Itai C,Benzioni A,Musolan C,Kindinger JI

    更新日期:1974-01-01 00:00:00

  • Proteomic investigation of the effect of salicylic acid on Arabidopsis seed germination and establishment of early defense mechanisms.

    abstract::The influence of salicylic acid (SA) on elicitation of defense mechanisms in Arabidopsis (Arabidopsis thaliana) seeds and seedlings was assessed by physiological measurements combined with global expression profiling (proteomics). Parallel experiments were carried out using the NahG transgenic plants expressing the ba...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.082057

    authors: Rajjou L,Belghazi M,Huguet R,Robin C,Moreau A,Job C,Job D

    更新日期:2006-07-01 00:00:00

  • Endogenous NO(3) in the Root as a Source of Substrate for Reduction in the Light.

    abstract::An experiment was conducted to investigate the reduction of endogenous NO(3) (-), which had been taken up by plants in darkness, during the course of the subsequent light period. Vegetative, nonnodulated soybean plants (Glycine max [L]. Merrill, ;Ransom') were exposed to 1.0 millimolar (15)NO(3) (-) for 12 hours in da...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.4.1421

    authors: Rufty TW,Volk RJ,Mackown CT

    更新日期:1987-08-01 00:00:00

  • Estimation of Bundle Sheath Cell Conductance in C4 Species and O2 Insensitivity of Photosynthesis.

    abstract::Low conductance to CO2 of bundle sheath cells is required in C4 photosynthesis to maintain high [CO2] at the site of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Elevated [CO2] allows high CO2 assimilation rates by this enzyme and prevents Rubisco oxygenase activity and O2 inhibition of carboxylation. Bu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.4.1183

    authors: Brown RH,Byrd GT

    更新日期:1993-12-01 00:00:00

  • Germination, respiration, and adenylate energy charge of seeds at various oxygen partial pressures.

    abstract::The effect of O(2) partial pressure on the germination and the respiration of 12 cultivated species was studied. The reciprocal of the time necessary to observe rootlet emergence in 50% of the seeds was used to approach the germination rate. The maximum germination and respiration rates were reached in most seeds at O...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.3.885

    authors: Al-Ani A,Bruzau F,Raymond P,Saint-Ges V,Leblanc JM,Pradet A

    更新日期:1985-11-01 00:00:00

  • A brassinosteroid-hypersensitive mutant of BAK1 indicates that a convergence of photomorphogenic and hormonal signaling modulates phototropism.

    abstract::The phototropic response of Arabidopsis (Arabidopsis thaliana) is induced by the phototropin photoreceptors and modulated by the cryptochrome and phytochrome photoreceptors. Downstream of these photoreceptors, asymmetric lateral redistribution of auxin underlies the differential growth, which results in phototropism. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.064444

    authors: Whippo CW,Hangarter RP

    更新日期:2005-09-01 00:00:00

  • Role of the low-molecular-weight subunits PetL, PetG, and PetN in assembly, stability, and dimerization of the cytochrome b6f complex in tobacco.

    abstract::The cytochrome b(6)f (Cyt b(6)f) complex in flowering plants contains nine conserved subunits, of which three, PetG, PetL, and PetN, are bitopic plastid-encoded low-molecular-weight proteins of largely unknown function. Homoplastomic knockout lines of the three genes have been generated in tobacco (Nicotiana tabacum '...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.100131

    authors: Schwenkert S,Legen J,Takami T,Shikanai T,Herrmann RG,Meurer J

    更新日期:2007-08-01 00:00:00

  • Azolla-Anabaena azollae Relationship: IV. Photosynthetically Driven, Nitrogenase-catalyzed H(2) Production.

    abstract::The water fern, Azolla caroliniana Willd., containing the symbiotic, heterocystous blue-green alga, Anabaena azollae, has been studied under various growth conditions to characterize its light-dependent production of H(2). The response of H(2) production to N(2) and C(2)H(2) and the absence of a differential effect of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.2.119

    authors: Peters GA,Evans WR,Toia RE

    更新日期:1976-08-01 00:00:00

  • Light-induced growth promotion by SPA1 counteracts phytochrome-mediated growth inhibition during de-etiolation.

    abstract::Previous evidence has suggested that SPA1 is a signal transduction component that appears to require phytochrome A for function in seedling photomorphogenesis. Using digital image analysis, we examined the time course of growth inhibition induced by red light in spa1 mutants to test the interpretation that SPA1 functi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.3.1291

    authors: Parks BM,Hoecker U,Spalding EP

    更新日期:2001-07-01 00:00:00

  • Distinctive Light and CO(2)-Fixation Requirements of Nitrate and Ammonium Utilization by the Cyanobacterium Anacystis nidulans.

    abstract::The effect of light intensity on the rates of ammonium and nitrate uptake and of CO(2) fixation has been determined in intact Anacystis nidulans cells. Ammonium uptake became saturated at photon flux values of about 60 microeinsteins per square meter per second, whereas both nitrate uptake and CO(2) fixation reached s...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.2.686

    authors: Lara C,Romero JM

    更新日期:1986-06-01 00:00:00

  • Inhibition of Acid-Enhanced Elongation of Zea mays Root Segments by Galactose.

    abstract::The effect of sugars and metabolic inhibitors on the elongation of Zea mays root segments was analyzed by a rhizometer which records the elongation of each of 32 root segments at the same time. Galactose suppressed the acid-enhanced rapid elongation after a lag period of 1.5 hours, but it did not inhibit the slow elon...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.2.440

    authors: Tanimoto E,Scott TK,Masuda Y

    更新日期:1989-06-01 00:00:00

  • The Azolla-Anabaena azollae Relationship : XI. PHYCOBILIPROTEINS IN THE ACTION SPECTRUM FOR NITROGENASE-CATALYZED ACETYLENE REDUCTION.

    abstract::Visible absorption spectra are presented for the Azolla caroliniana Willd.-Anabaena azollae Strass. association and the individual partners. Although absorption by the phycobiliproteins of the endophytic cyanobacterium clearly complements the absorption by the fern pigments, their contribution to the absorption spectr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.6.1479

    authors: Tyagi VV,Ray TB,Mayne BC,Peters GA

    更新日期:1981-12-01 00:00:00

  • Cadmium uptake kinetics in intact soybean plants.

    abstract::The absorption characteristics of Cd(2+) by 10- to 12-day-old soybean plants (Glycine max cv Williams) were investigated with respect to influence of Cd concentration on adsorption to root surfaces, root absorption, transport kinetics and interaction with the nutrient cations Cu(2+), Fe(2+), Mn(2+), and Zn(2+). The fr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.3.844

    authors: Cataldo DA,Garland TR,Wildung RE

    更新日期:1983-11-01 00:00:00

  • 3-Methyleneoxindole reductase of peas.

    abstract::A 100-fold purification of a reduced triphosphopyridine nucleotide/3-methyleneoxindole reductase of peas has been achieved using conventional protein fractionation procedures. Reduced diphosphopyridine nucleotide is 25-fold less effective than reduced triphosphopyridine nucleotide as the reductant. The preparation is ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.4.510

    authors: Moyed HS,Williamson V

    更新日期:1967-04-01 00:00:00

  • Effects of temperature, water potential, and light on germination responses of redroot pigweed seeds to ethylene.

    abstract::The responses of redroot pigweed (Amaranthus retroflexus L.) seeds to nine ethylene concentrations between 0.5 and 50 microliters per liter were assessed at different temperatures and water potentials and in either continuous white light or darkness. Under all experimental treatments, the probit-transformed percentage...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.6.1149

    authors: Schonbeck MW,Egley GH

    更新日期:1980-06-01 00:00:00

  • Cellulase Activity, Endogenous Abscisic Acid, and Ethylene in Four Citrus Cultivars during Maturation.

    abstract::At maturity, the fruit of two early maturing orange cultivars, Hamlin and Pineapple (Citrus sinensis [L.] Osbeck), contained more ethylene and abscisic acid than the late maturing Valencia and Lamb Summer (C. sinensis [L.] Osbeck) cultivars. Ethylene (up to 95 nl/l in internal atmosphere) and abscisic (50 mug/kg dry w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.56.6.765

    authors: Rasmussen GK

    更新日期:1975-12-01 00:00:00