Abstract:
:A previously isolated rice (Oryza sativa) rbcS gene was further characterized. This analysis revealed specific sequences in the 5' regulatory region of the rice rbcS gene that are conserved in rbcS genes of other monocotyledonous species. In transgenic rice plants, we examined the expression of the beta-glucuronidase (gusA) reporter gene directed by the 2.8-kb promoter region of the rice rbcS gene. To examine differences in the regulation of monocotyledonous and dicotyledonous rbcS promoters, the activity of a tomato rbcS promoter was also investigated in transgenic rice plants. Our results indicated that both rice and tomato rbcS promoters confer mesophyll-specific expression of the gusA reporter gene in transgenic rice plants and that this expression is induced by light. However, the expression level of the rice rbcS-gusA gene was higher than that of the tomato rbcS-gusA gene, suggesting the presence of quantitative differences in the activity of these particular monocotyledonous and dicotyledonous rbcS promoters in transgenic rice. Histochemical analysis of rbcS-gusA gene expression showed that the observed light induction was only found in mesophyll cells. Furthermore, it was demonstrated that the light regulation of rice rbcS-gusA gene expression was primarily at the level of mRNA accumulation. We show that the rice rbcS gene promoter should be useful for expression of agronomically important genes for genetic engineering of monocotyledonous species.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Kyozuka J,McElroy D,Hayakawa T,Xie Y,Wu R,Shimamoto Kdoi
10.1104/pp.102.3.991subject
Has Abstractpub_date
1993-07-01 00:00:00pages
991-1000issue
3eissn
0032-0889issn
1532-2548pii
102/3/991journal_volume
102pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Sucrose synthase (EC 2.4.1.13) of sugarbeet (Beta vulgaris L.) fibrous roots has a distinctly different order of preference for nucleoside diphosphate substrates than that of the taproot enzyme (Silvius, Snyder 1979 Plant Physiol 64: 1070-1073). Separation and quantitation of UDP, ADP, and GDP in root extracts by high...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.1.316
更新日期:1982-07-01 00:00:00
abstract::We have examined the effects of the substituted pyridazinone herbicide, 4-chloro-5-(dimethylamino)-2-phenyl-3(2H)pyridazinone (BASF 13-338, Sandoz 9785), on the desaturation of linoleic acid (18:2) on different molecular species of monogalactosyldiacylglycerol (MGDG) and phosphatidylcholine (PC) in leaf tissue of Arab...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.3.684
更新日期:1987-11-01 00:00:00
abstract::Treatment of Pisum sativum (L.) cv. ;Sparkle' with ethyl methanesulfonic acid (EMS) produced a stable mutant, E135F, which forms small, white, ineffective nodules. These nodules exhibit histological zonation typical of an indeterminant nodule, e.g. meristematic, early symbiotic, late symbiotic, and senescent zones. Co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.3.899
更新日期:1990-11-01 00:00:00
abstract::Chlorophyll degradation plays important roles in leaf senescence including regulation of degradation of chlorophyll-binding proteins. Although most genes encoding enzymes of the chlorophyll degradation pathway have been identified, the regulation of their activity has not been fully understood. Green cotyledon mutants...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01589
更新日期:2017-04-01 00:00:00
abstract::NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ubiquitous enzyme involved in the glycolytic pathway. It has been widely demonstrated that mammalian GAPDH, in addition to its role in glycolysis, fulfills alternative functions mainly linked to its susceptibility to oxidative posttranslational modifi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.215194
更新日期:2013-05-01 00:00:00
abstract::The odoriferous compounds produced at the time of flowering by the inflorescences of 5 arum lily species were condensed in dry ice traps. The components of the condensates were separated by paper chromatography and identified on the basis of position and color reactions in comparison with known compounds. The concentr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.2.343
更新日期:1966-02-01 00:00:00
abstract::In maize (Zea mays L., cv Contessa), nitrogen (NO(3) (-)) limitation resulted in a reduction in shoot growth and photosynthetic capacity and in an increase in the leaf zeaxanthin contents. Nitrogen deficiency had only a small effect on the quantum yield of CO(2) assimilation but a large effect on the light-saturated r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.3.1436
更新日期:1990-11-01 00:00:00
abstract::A mass spectrometric method combining (16)O/(18)O and (12)C/(13)C isotopes was used to quantify the unidirectional fluxes of O(2) and CO(2) during a dark to light transition for guard cell protoplasts and mesophyll cell protoplasts of Commelina communis L. In darkness, O(2) uptake and CO(2) evolution were similar on a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.95.2.636
更新日期:1991-02-01 00:00:00
abstract::The petunia (Petunia [Mitchell]) chloroplast proteins, the chlorophyll a/b-binding (Cab) proteins, and the small subunit of ribulose bisphosphate carboxylase (RbcS) are encoded by nuclear genes that are expressed in a light-dependent manner. The steady-state concentrations of five cab mRNAs vary with a dramatic circad...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.3.776
更新日期:1989-03-01 00:00:00
abstract::Onion (Allium cepa L.) bulbs were frozen to -4 and -11 C and kept frozen for up to 12 days. After slow thawing, a 2.5-cm square from a bulb scale was transferred to 25 ml deionized H(2)O. After shaking for standard times, measurements were made on the effusate and on the effused cells. The results obtained were as fol...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.3.393
更新日期:1977-09-01 00:00:00
abstract::A novel Acremonium typhinum proteinase that is expressed during endophytic infection of the grass Poa ampla Merr. was purified from endophyte-infected leaf sheath tissue. It is a thiol-containing serine alkaline endoproteinase with bound carbohydrate. In the infected host tissue, this proteinase is an abundant protein...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.1.7
更新日期:1994-09-01 00:00:00
abstract::To investigate the relation between cell division and expansion in the regulation of organ growth rate, we used Arabidopsis thaliana primary roots grown vertically at 20 degreesC with an elongation rate that increased steadily during the first 14 d after germination. We measured spatial profiles of longitudinal veloci...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.116.4.1515
更新日期:1998-04-01 00:00:00
abstract::Plant shoot organs arise from initial cells that are recruited from meristematic tissues. Previous studies have shown that members of the WUSCHEL-related HOMEOBOX (WOX) gene family function to organize various initial cell populations during plant development. The function of the WOX4 gene is previously undescribed in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.149641
更新日期:2010-03-01 00:00:00
abstract::We have shown that amide-linked IAA (indole-3-acetic acid) conjugates accumulated to high levels during maturation of bean seeds (K. Bialek and J.D. Cohen [1989] Plant Physiol 91: 775-779). In the present study, we were interested in the fate of these and other IAA conjugates during seed germination. The content of am...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.4.2002
更新日期:1992-12-01 00:00:00
abstract::We have analyzed gene regulation of the Lhc supergene family in poplar (Populus spp.) and Arabidopsis (Arabidopsis thaliana) using digital expression profiling. Multivariate analysis of the tissue-specific, environmental, and developmental Lhc expression patterns in Arabidopsis and poplar was employed to characterize ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.073304
更新日期:2006-03-01 00:00:00
abstract::In immature rib segments prepared from morning-glory (Ipomoea tricolor) flower buds, the major soluble metabolite formed from tracer amounts of l-methionine-U-(14)C was S-methylmethionine (SMM). In segments of senescing ribs, (14)C was progressively lost from SMM and appeared in free methionine. Immature segments cont...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.57.4.528
更新日期:1976-04-01 00:00:00
abstract::As part of an extensive analysis of the factors regulating photosynthesis in Agropyron smithii Rydb., a C(3) grass, we have examined the response of leaf gas exchange and ribulose-1,5-bisphosphate (RuBP) carboxylase activity to temperature. Emphasis was placed on elucidating the specific processes which regulate the t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.4.921
更新日期:1982-04-01 00:00:00
abstract::The mineral nutrient requirements of Pinus taeda cells were explored using quantitative cell culture growth measurements. An appraisal was thereby made of the critical features of a novel and successful medium which was developed specifically for this gymnosperm using chemical composition data for developing seeds, an...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.4.942
更新日期:1986-12-01 00:00:00
abstract::The capacity of excised internode sections of pea to grow and secrete protons in response to indoleacetic acid (IAA) and Ca(2+) and K(+) treatments was examined. By incubating unpeeled and unabraded sections in rapidly flowing solutions, it was shown that acidification of the external medium in the presence or absence...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.1.59
更新日期:1981-07-01 00:00:00
abstract::DNA and histone methylation coregulate heterochromatin formation and gene silencing in animals and plants. To identify factors involved in maintaining gene silencing, we conducted a forward genetic screen for mutants that release the silenced transgene Pro35S::NEOMYCIN PHOSPHOTRANSFERASE II in the transgenic Arabidops...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00183
更新日期:2018-06-01 00:00:00
abstract::We present immunocytochemical localizations of four enzymes involved in p-menthane monoterpene biosynthesis in mint: the large and small subunits of peppermint (Mentha x piperita) geranyl diphosphate synthase, spearmint (Mentha spicata) (-)-(4S)-limonene-6-hydroxylase, peppermint (-)-trans-isopiperitenol dehydrogenase...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.050229
更新日期:2004-12-01 00:00:00
abstract::Regulation of cortical microtubule reorganization is essential for plant cell survival under high salinity conditions. In response to salt stress, microtubules undergo rapid depolymerization followed by reassembly to form a new microtubule network that promotes cell survival; however, the upstream regulatory mechanism...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01124
更新日期:2018-03-01 00:00:00
abstract::Apical dominance is the process whereby the shoot tip inhibits the growth of axillary buds along the stem. It has been proposed that the shoot tip, which is the predominant source of the plant hormone auxin, prevents bud outgrowth by suppressing auxin canalization and export from axillary buds into the main stem. In t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00519
更新日期:2019-01-01 00:00:00
abstract::The plant hormone jasmonic acid (JA) plays a pivotal role in plant-insect interactions. Herbivore attack usually elicits dramatic increases in JA concentrations, which in turn activate the accumulation of metabolites that function as defenses against herbivores. Although almost all enzymes involved in the biosynthesis...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.199018
更新日期:2012-08-01 00:00:00
abstract::The action of the polyene antibiotic filipin on the permeability of liposomes prepared with lecithin and several plant and other steroids was studied. The effect of filipin was found to be dependent upon the type of steroid incorporated into the membrane. The interaction of filipin with steroids was found to be relate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.5.852
更新日期:1972-05-01 00:00:00
abstract::Soybean (Glycine max L. Merr.) is a versatile and important agronomic crop grown worldwide. Each year millions of dollars of potential yield revenues are lost due to a root rot disease caused by the oomycete Phytophthora sojae (Kaufmann & Gerdemann). Since the root is the primary site of infection by this organism, we...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.091090
更新日期:2007-05-01 00:00:00
abstract::A number of parameters associated with the isolation of plant mitochondria were carefully examined in the hope of optimizing the isolation technique. Dark-grown mung bean (Phaseolus aureus var. Jumbo) hypocotyls were used as the source of mitochondria. Necessary conditions for the isolation of tightly coupled plant mi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.45.6.773
更新日期:1970-06-01 00:00:00
abstract::The class III, ma(3) (R)-containing maturity genotypes of Sorghum bicolor (L.) Moench differ from all of the other maturity genotypes in that they flower earlier, exhibit rapid shoot elongation sooner after floral differentiation, and exhibit several morphological differences. In the present study, a class III, ma(3) ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.2.581
更新日期:1986-10-01 00:00:00
abstract::The fhy3 mutation of Arabidopsis impairs phytochrome A (phyA)-mediated inhibition of hypocotyl growth without affecting the levels of phyA measured spectrophotometrically or immunochemically. We investigated whether the fhy3-1 mutation has similar effects on very low fluence responses (VLFR) and high irradiance respon...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.1.235
更新日期:2000-05-01 00:00:00
abstract::In fully expanded leaves of greenhouse-grown cotton (Gossypium hirsutum L., cv Coker 100) plants, carbon export, starch accumulation rate, and carbon exchange rate exhibited different behavior during the light period. Starch accumulation rates were relatively constant during the light period, whereas carbon export rat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.2.584
更新日期:1986-06-01 00:00:00