Abstract:
:The present studies provide the first measurements of the resistance to diffusive flux of metabolites between mesophyll and bundle sheath cells of C(4) plants. Species examined were Panicum miliaceum, Urochloa panicoides, Atriplex spongiosa, and Zea mays. Diffusive flux of metabolites into isolated bundle sheath cells was monitored by following their metabolic transformation. Evidence was obtained that the observed rapid fluxes occurred via functional plasmodesmata. Diffusion constants were determined from the rate of transformation of limiting concentrations of metabolites via cytosolic enzymes with high potential velocities and favorable equilibrium constants. Values on a leaf chlorophyll basis ranged between 1 and 5 micromoles per minute per milligram of chlorophyll per millimolar gradient depending on the molecular weight of the metabolite and the source of bundle sheath cells. Diffusion of metabolites into these cells was unaffected by a wide variety of compounds including respiratory inhibitors, monovalent and divalent cations, and plant hormones, but it was interrupted by treatments inducing cell plasmolysis. The molecular weight exclusion limit for permeation of compounds into bundle sheath cells was in the range of 850 to 900. These cells provide an ideal system for the quantitative study of plasmodesmatal function.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Weiner H,Burnell JN,Woodrow IE,Heldt HW,Hatch MDdoi
10.1104/pp.88.3.815subject
Has Abstractpub_date
1988-11-01 00:00:00pages
815-22issue
3eissn
0032-0889issn
1532-2548journal_volume
88pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The emergence of sterile individuals in the hybrid backcross progeny of wild and cultivated rice limits the use of wild rice alleles for improving cultivated rice, but the molecular mechanisms underlying this sterility remain unclear. Here, we identified the semisterile introgression line YIL42, derived from a cross b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.01374
更新日期:2019-05-01 00:00:00
abstract::Freshly isolated cotyledons from 10-day developing pea (Pisum sativum) seeds were fed radiolabeled precursors for 5 hours, and the specific radioactivity of the free and total protein amino acids was determined using a dansylation procedure. When the seven most abundant amino acids in phloem exudate of pea fruits (asp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.3.344
更新日期:1977-09-01 00:00:00
abstract::The partitioning of noncyclic photosynthetic electron transport between net fixation of CO(2) and collective O(2)-dependent, dissipative processes such as photorespiration has been examined in intact leaf tissue from Nicotiana tabacum. The method involves simultaneous application of CO(2) exchange and pulse modulated ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.4.1322
更新日期:1989-08-01 00:00:00
abstract::Rhythmic fluctuation in the basipetal movement of auxin occurs in corn (Zea mays) coleoptiles oriented either in the vertical or in the horizontal position. This periodicity of transport rate varies from region to region in a horizontal coleoptile. Between an upper and lower half coleoptile (with respect to gravity), ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.2.166
更新日期:1973-08-01 00:00:00
abstract::The effects of plant hormones and sucrose (Suc) on potato (Solanum tuberosum L.) tuberization were studied using in vitro cultured single-node cuttings. Tuber-inducing (high Suc) and -noninducing (low Suc or high Suc plus gibberellin [GA]) media were tested. Tuberization frequencies, tuber widths, and stolon lengths w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.2.575
更新日期:1998-06-01 00:00:00
abstract::The incorporation of adenosine-8-(14)C into adenosine cyclic 3':5'-monophosphate in coleoptile-first leaf segments of Avena sativa L. was investigated. Homogenates of segments incubated in adenosine-8-(14)C for either 4 or 10 hours were partially purified by thin layer chromatography followed by paper electrophoresis....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.2.346
更新日期:1975-02-01 00:00:00
abstract::Investigation was made on the effect of partial depletion of polyamines (PAs), induced by treatment with inhibitors of the biosynthesis of PAs, on the distribution of cells at each phase of the cell cycle in Catharanthus roseus (L.) G. Don. cells in suspension cultures, using flow cytometry. More cells treated with in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.4.1008
更新日期:1991-08-01 00:00:00
abstract::Gravitropic asymmetry of auxin was observed in hypocotyls of tomato (Lycopersicon esculentum Mill.) soon after horizontal placement: the ratio of apically supplied [3H]IAA collected from the lower sides to that from the upper sides was about 1.4 between 5 and 10 minutes. This was adequately early to account for the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.2.652
更新日期:1989-01-01 00:00:00
abstract::Plants, algae, and cyanobacteria have developed mechanisms to decrease the energy arriving at reaction centers to protect themselves from high irradiance. In cyanobacteria, the photoactive Orange Carotenoid Protein (OCP) and the Fluorescence Recovery Protein are essential elements in this mechanism. Absorption of stro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00843
更新日期:2015-09-01 00:00:00
abstract::The Craig-Gordon evaporative enrichment model of the hydrogen (deltaD) and oxygen (delta(18)O) isotopes of water was tested in a controlled-environment gas exchange cuvette over a wide range (400 per thousand deltaD and 40 per thousand delta(18)O) of leaf waters. (Throughout this paper we use the term "leaf water" to ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.120.4.1165
更新日期:1999-08-01 00:00:00
abstract::The presence of multiple forms of alpha-amylase in gibberellic acid-treated embryoless barley half-seeds was demonstrated by separation on diethylaminoethyl-Sephadex and isoelectric focusing polyacrylamide gel disc electrophoresis. Two major alpha-amylase fractions (A and B), each consisting of two to three isozyme co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.46.4.586
更新日期:1970-10-01 00:00:00
abstract::Plants possess many metabolic genes for the production of a wide variety of phytochemicals in a tissue-specific manner. However, the metabolic systems behind the diversity and tissue-dependent regulation still remain unknown due to incomplete characterization of phytochemicals produced in a single plant species. Thus,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.148031
更新日期:2010-02-01 00:00:00
abstract::The plant hormone jasmonic acid (JA) plays a pivotal role in plant-insect interactions. Herbivore attack usually elicits dramatic increases in JA concentrations, which in turn activate the accumulation of metabolites that function as defenses against herbivores. Although almost all enzymes involved in the biosynthesis...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.199018
更新日期:2012-08-01 00:00:00
abstract::We report the molecular cloning and sequence of the cDNA coding for the biotin-containing subunit of the chloroplastic acetylcoenzyme A (CoA) carboxylase (ACCase) of Arabidopsis thaliana (CAC1). The 3' end of the CAC1 sequence, coding for a peptide of 94 amino acids, which includes a putative biotinylation motif, was ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.2.619
更新日期:1995-10-01 00:00:00
abstract::Ferns (Pteridophyta) are very important members of the plant kingdom that lag behind other taxa with regards to our understanding of their genetics, genomics, and molecular biology. We report here, to our knowledge, the first instance of stable transformation of fern with recovery of transgenic sporophytes. Spores of ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.224675
更新日期:2013-10-01 00:00:00
abstract::Increased ethylene synthesis was rapidly induced throughout the apical meristematic region of etiolated seedlings of Pisum sativum L., cv. Alaska by cuts made 1 centimeter from the apical hook. The wound signal was transmitted at about 2 millimeters per minute. Accumulation of substance(s) at the cut surfaces of excis...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.62.5.710
更新日期:1978-11-01 00:00:00
abstract::Following dark adaptation, the response to irradiance of chlorophyll (Chl) fluorescence, the light-induced absorbance change around 820 nm (to measure reaction center Chl of photosystem I [PSI] P-700 oxidation), and CO2 fixation were examined in pea (Pisum sativum L.) leaves under a range of conditions. Initially, P-7...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.2.649
更新日期:1993-10-01 00:00:00
abstract::Plant mitochondria contain non-phosphorylating NAD(P)H dehydrogenases (DHs) that are not found in animal mitochondria. The physiological function, substrate specificity, and location of enzymes within this family have yet to be conclusively determined. We have linked genome sequence information to protein and biochemi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.029363
更新日期:2003-12-01 00:00:00
abstract::Phenylpropanoid biosynthesis in plants engenders myriad phenolics with diverse biological functions. Phenylalanine ammonia-lyase (PAL) is the first committed enzyme in the pathway, directing primary metabolic flux into a phenylpropanoid branch. Previously, we demonstrated that the Arabidopsis (Arabidopsis thaliana) Ke...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.249136
更新日期:2015-02-01 00:00:00
abstract::Partitioning and translocation of (14)C-photosynthates were examined during flowering and seed maturation in soybean (Glycine max [L.]Merr.) plants to quantify allocation to sugars, amino acids, organic acids, and starch and to study transport of C and N from leaves to reproductive sinks. The trifoliolate leaf at the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.4.1040
更新日期:1984-08-01 00:00:00
abstract::The cell separation aspect of abscission is thought to involve the action of specific cell wall degrading enzymes. Enzymes represent synthesis which in turn is preceded by the synthesis of specific RNA molecules, and it follows that inhibition of either of these processes would also block abscission. Since abscission ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:
更新日期:1968-09-01 00:00:00
abstract::Roots of Fe-sufficient and Fe-Deficient pea (Pisum sativum L.) were studied to determine the effect of Fe-deficiency on the activity of the root-cell plasmalemma Fe(2+) transport protein. Rates of Fe(III) reduction and short-term Fe(2+) influx were sequentially determined in excised primary lateral roots using Fe(III)...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.3.1353
更新日期:1990-11-01 00:00:00
abstract::The mobilization of stored carbohydrates (sucrose and starch) and proteins during sucrose starvation was studied with sycamore (Acer pseudoplatanus L.) cells. When almost all the intracellular carbohydrate pools had disappeared, the cell protein content declined progressively whereas asparagine determined by either (1...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.2.717
更新日期:1990-10-01 00:00:00
abstract::C-TERMINALLY ENCODED PEPTIDEs (CEPs) control root system architecture in a non-cell-autonomous manner. In Medicago truncatula, MtCEP1 affects root development by increasing nodule formation and inhibiting lateral root emergence by unknown pathways. Here, we show that the MtCEP1 peptide-dependent increase in nodulation...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00113
更新日期:2016-08-01 00:00:00
abstract::Leaf discs and detached leaves exposed to l-cysteine emitted a volatile sulfur compound which was proven by gas chromatography to be H(2)S. This phenomenon was demonstrated in all nine species tested (Cucumis sativus, Cucurbita pepo, Nicotiana tabacum, Coleus blumei, Beta vulgaris, Phaseolus vulgaris, Medicago sativa,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.2.430
更新日期:1982-08-01 00:00:00
abstract::Iron (Fe) is an essential mineral nutrient and an important factor for the composition of natural plant communities. Low Fe availability in aerated soils with neutral or alkaline pH has led to the evolution of elaborate mechanisms that extract Fe from the soil solution. In Arabidopsis (Arabidopsis thaliana), Fe is acq...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00178
更新日期:2018-05-01 00:00:00
abstract::The absorption characteristics of Cd(2+) by 10- to 12-day-old soybean plants (Glycine max cv Williams) were investigated with respect to influence of Cd concentration on adsorption to root surfaces, root absorption, transport kinetics and interaction with the nutrient cations Cu(2+), Fe(2+), Mn(2+), and Zn(2+). The fr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.3.844
更新日期:1983-11-01 00:00:00
abstract::Fluorescence emission spectral peaks at 685, 695 and 730 nanometers (F685, F695, and F730) were recorded 77 K from diluted leaf tissue and epidermal powders prepared from Saxifraga cernua. The time course for state 1 to state 2 transitions was monitored as changes in the ratios of the three emission peaks. During illu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.4.873
更新日期:1986-12-01 00:00:00
abstract::Accumulating evidence indicates that there is a multiplicity of cytochrome P-450 enzymes in plants. These monooxygenases are implicated in the metabolism of sterols, terpenes, gibberellins, isoflavonoids, and xenobiotics. Evidence that cytochromes P-450 are involved in the detoxification of herbicides (chlorotoluron, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.3.669
更新日期:1991-07-01 00:00:00
abstract::Plant endosperm cells have a nuclear ratio of two maternal genomes to one paternal genome. This 2 to 1 dosage relationship provides a unique system for studying the additivity of gene expression levels in reciprocal hybrids. A combination of microarray profiling and allele-specific expression analysis was performed us...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.101428
更新日期:2007-10-01 00:00:00