Abstract:
:Iron (Fe) is an essential mineral nutrient and an important factor for the composition of natural plant communities. Low Fe availability in aerated soils with neutral or alkaline pH has led to the evolution of elaborate mechanisms that extract Fe from the soil solution. In Arabidopsis (Arabidopsis thaliana), Fe is acquired by an orchestrated strategy that comprises mobilization, chelation, and reduction of Fe3+ prior to its uptake. Here, we show that At3g12900, previously annotated as scopoletin 8-hydroxylase (S8H), participates in Fe acquisition by mediating the biosynthesis of fraxetin (7,8-dihydroxy-6-methoxycoumarin), a coumarin derived from the scopoletin pathway. S8H is highly induced in roots of Fe-deficient plants both at the transcript and protein levels. Mutants defective in the expression of S8H showed increased sensitivity to growth on pH 7.0 media supplemented with an immobile source of Fe and reduced secretion of fraxetin. Transgenic lines overexpressing S8H exhibited an opposite phenotype. Homozygous s8h mutants grown on media with immobilized Fe accumulated significantly more scopolin, the storage form of scopoletin, supporting the designated function of S8H in scopoletin hydroxylation. Fraxetin exhibited Fe-reducing properties in vitro with higher rates being observed at neutral relative to acidic pH. Supplementing the media containing immobile Fe with fraxetin partially rescued the s8h mutants. In natural Arabidopsis accessions differing in their performance on media containing immobilized Fe, the amount of secreted fraxetin was highly correlated with growth and Fe and chlorophyll content, indicating that fraxetin secretion is a decisive factor for calcicole-calcifuge behavior (i.e. the ability/inability to thrive on alkaline soils) of plants.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Tsai HH,Rodríguez-Celma J,Lan P,Wu YC,Vélez-Bermúdez IC,Schmidt Wdoi
10.1104/pp.18.00178subject
Has Abstractpub_date
2018-05-01 00:00:00pages
194-207issue
1eissn
0032-0889issn
1532-2548pii
pp.18.00178journal_volume
177pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::We examined cell length, mitosis, and root meristem "cuticle" in different tissues of geostimulated, red light-exposed primary roots of corn (Zea Mays, Wisconsin hybrid 64A x 22R). The examination was done at 15-minute intervals for a period of 240 minutes. Differences in cell elongation between the upper and lower si...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.1.7
更新日期:1978-01-01 00:00:00
abstract::Synechocystis (PCC 6714) is photoinhibited at low photon flux density when illuminated in medium devoid of Na(+). Photosynthetic activity and variable fluorescence yield were measured in intact cells during the course of inhibition and during recovery from inhibition in order to characterize sequential inhibitory even...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.1.91
更新日期:1989-09-01 00:00:00
abstract::Ethylene supplied with indoleacetic acid at 0.1 and 1 mum inhibited elongation and enhanced swelling in epicotyls of decapitated and derooted pea seedlings (Pisum sativum L., var. Alaska). These growth responses were correlated with the development of cell walls rich in weak acid-extractable materials and pectic uroni...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.3.450
更新日期:1975-03-01 00:00:00
abstract::A full-genome microarray of the (oxy)photosynthetic cyanobacterium Synechocystis sp. PCC 6803 was used to identify genes that were transcriptionally regulated by growth in iron (Fe)-deficient versus Fe-sufficient media. Transcript accumulation for 3,165 genes in the genome was analyzed using an analysis of variance mo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.024018
更新日期:2003-08-01 00:00:00
abstract::Two active calcium (Ca(2+)) transport systems have been identified and partially characterized in membrane vesicles isolated from cultured carrot cells (Daucus carota Danvers). Both transport systems required MgATP for activity and were enhanced by 10 millimolar oxalate. Ca(2+) transport in membrane vesicles derived f...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.2.549
更新日期:1986-02-01 00:00:00
abstract::Experiments were conducted with segments of corn roots to investigate whether nitrate reductase (NR) is compartmentalized in particular groups of cells that collectively form the root symplastic pathway. A microsurgical technique was used to separate cells of the epidermis, of the cortex, and of the stele. The presenc...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.3.675
更新日期:1986-11-01 00:00:00
abstract::A rapid and simple, large-scale method for the purification of DNA-dependent RNA polymerase III (EC 2.7.7.6) from wheat germ is presented. The method involves enzyme extraction at low ionic strength, polyethyleneimine fractionation, (NH(4))(2)SO(4) precipitation, and chromatography on DEAE-Sepharose CL-6B, DEAE-cellul...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.3.438
更新日期:1981-03-01 00:00:00
abstract::During the early stages of seed development, Arabidopsis (Arabidopsis thaliana) endosperm is syncytial and proliferates rapidly through repeated rounds of mitosis without cytokinesis. This stage of endosperm development is important in determining final seed size and is a model for studying aspects of cellular and mol...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.128108
更新日期:2008-12-01 00:00:00
abstract::How genes change their expression patterns over time is still poorly understood. Here, by conducting expression, functional, bioinformatic, and evolutionary analyses, we demonstrate that the differences between the Arabidopsis (Arabidopsis thaliana) APETALA1 (AP1) and CAULIFLOWER (CAL) duplicate genes in the time, spa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00320
更新日期:2016-06-01 00:00:00
abstract::The metabolism of d- and l-methionine by immature cotyledons of soybean (Glycine max, L. cv Elf) grown in culture has been investigated using solid-state (13)C and (15)N nuclear magnetic resonance. d-Methionine is taken up by the cotyledons and converted to an amide, most likely by N-malonylation. About 16% of the l-m...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.3.698
更新日期:1987-03-01 00:00:00
abstract::The release of fatty acids from membrane lipids has been implicated in various metabolic and physiological processes, but in many cases, the enzymes involved and their functions in plants remain unclear. Patatin-related phospholipase As (pPLAs) constitute a major family of acyl-hydrolyzing enzymes in plants. Here, we ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.216994
更新日期:2013-05-01 00:00:00
abstract::The halotolerant alga Dunaliella bardawil accumulates very large amounts of [beta]-carotene when exposed to high light intensity. The accumulated [beta]-carotene is concentrated in small, oily globules within the chloroplast and has been suggested to protect the alga against photodamage by high irradiation (A. Ben-Amo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.4.1657
更新日期:1995-08-01 00:00:00
abstract::A 3,300-bp DNA fragment encoding the carboxyl-transferase domain of the multidomain, chloroplastic acetyl-coenzyme A carboxylase (ACCase) was sequenced in aryloxyphenoxypropionate (APP)-resistant and -sensitive Alopecurus myosuroides (Huds.). No resistant plant contained an Ile-1,781-Leu substitution, previously shown...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.021139
更新日期:2003-07-01 00:00:00
abstract::Computing techniques are used to simulate the course of uptake of K(+), Na(+), and Cl(-) by low-salt roots. Measurements of the fluxes of these ions in high-salt roots are used to calculate membrane permeabilities, which are then used to calculate cell uptake. In this way it is possible to test the predictive value of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.10.1417
更新日期:1969-10-01 00:00:00
abstract::Disc electrophoresis was used to separate water soluble proteins from hardy, non-hardy, and frost killed cabbage (Brassica oleracea var. capitata) leaves. Amidoschwarz staining failed to reveal any new bands as a result of hardening although the relative amounts of proteins in individual bands changed. Sulfhydryl grou...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.2.168
更新日期:1969-02-01 00:00:00
abstract::We found similarities between the effects of low night temperatures (5 degrees C-10 degrees C) and slowly imposed water stress on photosynthesis in grapevine (Vitis vinifera L.) leaves. Exposure of plants growing outdoors to successive chilling nights caused light- and CO(2)-saturated photosynthetic O(2) evolution to ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.2.675
更新日期:1999-10-01 00:00:00
abstract::Membrane trafficking and cytoskeletal dynamics are important cellular processes that drive tip growth in root hairs. These processes interact with a multitude of signaling pathways that allow for the efficient transfer of information to specify the direction in which tip growth occurs. Here, we show that AGD1, a class...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.119529
更新日期:2008-08-01 00:00:00
abstract::Rubisco catalyzes the fixation of CO2 into organic compounds that are used for plant growth and the production of agricultural products, and specific sugar-phosphate derivatives bind tightly to the active sites of Rubisco, locking the enzyme in a catalytically inactive conformation. 2-carboxy-d-arabinitol-1-phosphate ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.00693
更新日期:2019-10-01 00:00:00
abstract::Hydrogen sulfide (H2S), a plant gasotransmitter, functions in the plant response to cadmium (Cd) stress, implying a role for cysteine desulfhydrase in producing H2S in this process. Whether d-CYSTEINE DESULFHYDRASE (DCD) acts in the plant Cd response remains to be identified, and if it does, how DCD is regulated in th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.01504
更新日期:2020-05-01 00:00:00
abstract::A screening procedure for identifying Cd-sensitive mutants of Arabidopsis thaliana is described. With this procedure, two Cd-sensitive mutants were isolated. These represent independent mutations in the same locus, referred to as CAD1. Genetic analysis has shown that the sensitive phenotype is recessive to the wild ty...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.1.100
更新日期:1992-09-01 00:00:00
abstract::A novel alliinase (EC 4.4.1.4) was detected and purified from the roots of the Amazonian medicinal plant Petiveria alliacea. The isolated enzyme is a heteropentameric glycoprotein composed of two alpha-subunits (68.1 kD each), one beta-subunit (56.0 kD), one gamma-subunit (24.8 kD), and one delta-subunit (13.9 kD). Th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.142430
更新日期:2009-11-01 00:00:00
abstract::Heat treatment (37 degrees C) of transgenic tobacco (Nicotiana tabacum) plants led to a reversible reduction or complete loss of transgene-encoded activities in about 40% of 10 independent transformants carrying the luciferase-coding region fused to the 355 cauliflower mosaic virus or the soybean small subunit promote...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.3.939
更新日期:1997-11-01 00:00:00
abstract::Leaf discs and detached leaves exposed to l-cysteine emitted a volatile sulfur compound which was proven by gas chromatography to be H(2)S. This phenomenon was demonstrated in all nine species tested (Cucumis sativus, Cucurbita pepo, Nicotiana tabacum, Coleus blumei, Beta vulgaris, Phaseolus vulgaris, Medicago sativa,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.2.430
更新日期:1982-08-01 00:00:00
abstract::The location and changes in NAD(P)H have been monitored during oscillatory growth in pollen tubes of lily (Lilium formosanum) using the endogenous fluorescence of the reduced coenzyme (excitation, 360 nm; emission, >400 nm). The strongest signal resides 20 to 40 microm behind the apex where mitochondria (stained with ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.087882
更新日期:2006-12-01 00:00:00
abstract::We compared nodule initiation in lateral roots of Pisum sativum (L.) cv Sparkle and in a low-nodulating mutant E2 (sym 5). In Sparkle, about 25% of the infections terminated in the epidermis, a similar number stopped in the cortex, and 50% resulted in the formation of a nodule meristem or an emerged nodule. The mutant...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.3.1206
更新日期:1991-11-01 00:00:00
abstract::The rhythmic movement of excised Samanea saman pulvini incubated in H(2)O or 50 mm sucrose was monitored during extended periods of white light (cool white fluorescent, 2,000 ft-c), darkness, or alternating white light (16 hr) and darkness (8 hr). In continuous white light, the rhythm damps at an intermediate angle af...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.3.417
更新日期:1976-09-01 00:00:00
abstract::The effect of short-term water stress on photosynthesis of two sunflower hybrids (Helianthus annuus L. cv Sungro-380 and cv SH-3622), differing in productivity under field conditions, was measured. The rate of CO(2) assimilation of young, mature leaves of SH-3622 under well-watered conditions was approximately 30% gre...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.2.516
更新日期:1992-02-01 00:00:00
abstract::The possible involvement of nucleic acid and protein synthesis in light-regulated chlorophyll formation by rapidly greening leaves has been studied.Removing leaves from illumination during the phase of rapid greening results in a reduction in the rate of pigment synthesis; cessation occurs within 2 to 4 hours. Etiolat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.6.774
更新日期:1967-06-01 00:00:00
abstract::The fine structure of the chloroplast of wild-type and 9 photosynthetic mutant strains of Chlamydomonas reinhardi is described. The chloroplast phenotypes of the mutant strains are clearly distinct from the wild type in all but 2 cases. Moreover, strains with similar photosynthetic disabilities have structurally simil...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.7.990
更新日期:1969-07-01 00:00:00
abstract::Surface glandular trichomes distributed throughout the aerial parts of sweet basil (Ocimum basilicum) produce and store monoterpene, sesquiterpene, and phenylpropene volatiles. Three distinct basil chemotypes were used to examine the molecular mechanisms underlying the divergence in their monoterpene and sesquiterpene...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.051318
更新日期:2004-11-01 00:00:00