Molecular cloning and characterization of the cDNA coding for the biotin-containing subunit of the chloroplastic acetyl-coenzyme A carboxylase.

Abstract:

:We report the molecular cloning and sequence of the cDNA coding for the biotin-containing subunit of the chloroplastic acetylcoenzyme A (CoA) carboxylase (ACCase) of Arabidopsis thaliana (CAC1). The 3' end of the CAC1 sequence, coding for a peptide of 94 amino acids, which includes a putative biotinylation motif, was expressed in Escherichia coli as a glutathione-S-transferase (GST) fusion protein. The resulting GST-CAC1 fusion protein was biotinylated in vivo, indicating that CAC1 codes for a biotin-containing protein. Antibodies generated to the GST-CAC1 protein reacted solely with the 38-kD biotin-containing polypeptide of Arabidopsis. Furthermore, these antibodies inhibited ACCase activity in extracts from Arabidopsis leaves. The deduced amino acid sequence of CAC1 has an apparent N-terminal chloroplast-targeting transit peptide. The CAC1 protein is coded by a single Arabidopsis gene, and its mRNA accumulates to the highest levels in organs that are undergoing rapid growth. The amino acid sequence of the CAC1 protein is most similar to the biotin carboxyl-carrier protein component of eubacterial ACCases. These characterizations identify CAC1 as the biotin-containing subunit of the plastidic, heteromeric ACCase of Arabidopsis. The results support the ancient origin of the two structurally distinct ACCases of plants.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Choi JK,Yu F,Wurtele ES,Nikolau BJ

doi

10.1104/pp.109.2.619

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

619-25

issue

2

eissn

0032-0889

issn

1532-2548

pii

109/2/619

journal_volume

109

pub_type

杂志文章
  • Characterization of a Giant PSI Supercomplex in the Symbiotic Dinoflagellate Symbiodiniaceae.

    abstract::Symbiodiniaceae are symbiotic dinoflagellates that provide photosynthetic products to corals. Because corals are distributed across a wide range of depths in the ocean, Symbiodiniaceae species must adapt to various light environments to optimize their photosynthetic performance. However, as few biochemical studies of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00726

    authors: Kato H,Tokutsu R,Kubota-Kawai H,Burton-Smith RN,Kim E,Minagawa J

    更新日期:2020-08-01 00:00:00

  • The Unfolded Protein Response Modulates a Phosphoinositide-Binding Protein through the IRE1-bZIP60 Pathway.

    abstract::Phosphoinositides function as lipid signals in plant development and stress tolerance by binding with partner proteins. We previously reported that Arabidopsis (Arabidopsis thaliana) phosphoinositide-specific phospholipase C2 functions in the endoplasmic reticulum (ER) stress response. However, the underlying molecula...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01488

    authors: Yu CY,Kanehara K

    更新日期:2020-05-01 00:00:00

  • Polyamines attenuate ethylene-mediated defense responses to abrogate resistance to Botrytis cinerea in tomato.

    abstract::Transgenic tomato (Solanum lycopersicum) lines overexpressing yeast spermidine synthase (ySpdSyn), an enzyme involved in polyamine (PA) biosynthesis, were developed. These transgenic lines accumulate higher levels of spermidine (Spd) than the wild-type plants and were examined for responses to the fungal necrotrophs B...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.188698

    authors: Nambeesan S,AbuQamar S,Laluk K,Mattoo AK,Mickelbart MV,Ferruzzi MG,Mengiste T,Handa AK

    更新日期:2012-02-01 00:00:00

  • Chloramphenicol as an energy transfer inhibitor in spinach chloroplasts.

    abstract::At concentrations of up to 300 mug/ml both d-threo- and l-threo-chloramphenicol act as energy transfer inhibitors in spinach chloroplasts, in that they inhibit both phosphorylation and phosphorylating electron transport, without affecting the nonphosphorylating electron transport which occurs either in the absence of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.5.691

    authors: Wara-Aswapati O,Bradbeer JW

    更新日期:1974-05-01 00:00:00

  • Root respiration associated with nitrogenase activity (c(2)h(2)) of soybean, and a comparison of estimates.

    abstract::Root respiration associated with symbiotic fixation in soybean (Glycine max [L.] Merr.) was estimated by four methods.Averaged over the life of the plant, the root respires 5.8 milligrams C per milligram N accumulated from fixation. When nitrogenase (C(2)H(2)) activity and root respiration were decreased by treating r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.3.701

    authors: Patterson TG,Larue TA

    更新日期:1983-07-01 00:00:00

  • Nitrogen Utilization in Lemna: I. Relations between Net Nitrate Flux, Nitrate Reduction, and in Vitro Activity and Stability of Nitrate Reductase.

    abstract::Cultures of Lemna gibba L. G3 were maintained at a constant, N-limited growth rate by adding nitrate daily in amounts calculated to sustain a rate of culture N increment of 0.20 day(-1). Nitrate added to the culture was consumed within 8 to 10 hours and the partitioning to reduction and accumulation during this phase ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.3.856

    authors: Ingemarsson B

    更新日期:1987-11-01 00:00:00

  • Nitrate Assimilation during Vegetative Regrowth of Alfalfa.

    abstract::Dry matter accumulation, nitrate reductase activity of various organs, nitrate accumulation, nitrogen derived from nitrate, and nitrogen content were studied during 17 days of vegetative regrowth of harvested (detopped) alfalfa (Medicago sativa L.). Seedlings were grown in the glasshouse and treated with 0, 40, and 80...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.5.1052

    authors: Vance CP,Heichel GH

    更新日期:1981-11-01 00:00:00

  • Partial Purification and Characterization of d-Ribose-5-phosphate Reductase from Adonis vernalis L. Leaves.

    abstract::This study presents evidence for a new enzyme, d-ribose-5-P reductase, which catalyzes the reaction: d-ribose-5-P + NADPH + H(+) --> d-ribitol-5-P + NADP(+). The enzyme was isolated from Adonis vernalis L. leaves in 38% yield and was purified 71-fold. The reductase was NADPH specific and had a pH optimum in the range ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.4.758

    authors: Negm FB,Marlow GC

    更新日期:1985-08-01 00:00:00

  • Bound auxin metabolism in cultured crown-gall tissues of tobacco.

    abstract::Bound auxin metabolism in cultured crown-gall tumor cells and pith callus of tobacco was examined by feeding radiolabeled auxins and auxin conjugates. In all tissues fed [(14)C]indoleacetic acid (IAA), at least one-third of the IAA was decarboxylated, and most of the remaining radiolabel occurred in a compound(s) whic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.2.315

    authors: Vijayaraghavan SJ,Pengelly WL

    更新日期:1986-02-01 00:00:00

  • Ionic Current Changes Associated with the Gravity-Induced Bending Response in Roots of Zea mays L.

    abstract::A vibrating probe was used to measure the changes in ionic currents around gravistimulated roots of Zea mays L. in an effort to determine whether these currents are involved in stimulus transduction from the root cap to the elongation zone. We did not observe a migration of the previously reported auxin-insensitive cu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.3.1417

    authors: Collings DA,White RG,Overall RL

    更新日期:1992-11-01 00:00:00

  • The C-Domain of Oleuropein β-Glucosidase Assists in Protein Folding and Sequesters the Enzyme in Nucleus.

    abstract::Oleuropein, a terpene-derived glycosylated secoiridoid biosynthesized exclusively by members of the Oleaceae family, is involved in a two-component defense system comprising a β-glucosidase that activates oleuropein into a toxic glutaraldehyde-like structure. Oleuropein and its deglycosylated derivatives have high pha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00512

    authors: Koudounas K,Thomopoulou M,Michaelidis C,Zevgiti E,Papakostas G,Tserou P,Daras G,Hatzopoulos P

    更新日期:2017-07-01 00:00:00

  • A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis.

    abstract::The PDF1.2 gene of Arabidopsis encoding a plant defensin is commonly used as a marker for characterization of the jasmonate-dependent defense responses. Here, using PDF1.2 promoter-deletion lines linked to the beta-glucoronidase-reporter gene, we examined putative promoter elements associated with jasmonate-responsive...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.017814

    authors: Brown RL,Kazan K,McGrath KC,Maclean DJ,Manners JM

    更新日期:2003-06-01 00:00:00

  • Heterogeneous distribution of glycosyltransferases in the endoplasmic reticulum of castor bean endosperm.

    abstract::Endoplasmic reticulum membranes stripped of attached ribosomes were isolated from homogenates of germinating castor bean (Ricinus communis L.) endosperm by sucrose density gradient centrifugation. The isolated endoplasmic reticulum fraction was further separated into two major membrane subfractions by centrifugation o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.2.547

    authors: Conder MJ,Lord JM

    更新日期:1983-06-01 00:00:00

  • The last step in cocaine biosynthesis is catalyzed by a BAHD acyltransferase.

    abstract::The esterification of methylecgonine (2-carbomethoxy-3β-tropine) with benzoic acid is the final step in the biosynthetic pathway leading to the production of cocaine in Erythoxylum coca. Here we report the identification of a member of the BAHD family of plant acyltransferases as cocaine synthase. The enzyme is capabl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.248187

    authors: Schmidt GW,Jirschitzka J,Porta T,Reichelt M,Luck K,Torre JC,Dolke F,Varesio E,Hopfgartner G,Gershenzon J,D'Auria JC

    更新日期:2015-01-01 00:00:00

  • Sugar regulates mRNA abundance of H(+)-ATPase gene family members in tomato.

    abstract::The plant plasma membrane H(+)-ATPase energizes the secondary uptake of nutrients and may facilitate cell expansion by acidifying the cell wall. In yeast, Glc stimulates the accumulation of H(+)-ATPase mRNA, and the growth rate supported by various sugars is correlated with H(+)-ATPase protein abundance. Expression of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.3.1229

    authors: Mito N,Wimmers LE,Bennett AB

    更新日期:1996-11-01 00:00:00

  • Metabolism of Glutamic Acid and N-Acetylglutamic Acid in Leaf Discs and Cell-free Extracts of Higher Plants.

    abstract::Radioactive glutamic acid and N-acetylglutamic acid have been incubated with normal and wilted leaf discs and radioactivity recovered in uncombined proline. The discs which had been placed under moisture stress incorporated considerably more label in uncombined proline than did normal discs.In extracts of swiss chard ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.7.1023

    authors: Morris CJ,Thompson JF,Johnson CM

    更新日期:1969-07-01 00:00:00

  • Cyanide-insensitive and Cyanide-sensitive O(2) Uptake in Wheat: II. GRADIENT-PURIFIED MITOCHONDRIA LACK CYANIDE-INSENSITIVE RESPIRATION.

    abstract::Enriched mitochondrial fractions isolated from durum wheat seedlings via differential centrifugation exhibited classical cyanide- or antimycin A-insensitive O(2) uptake which was inhibited by either salicylhydroxamic acid or propyl gallate. Further purification of this fraction using Percoll density gradients resulted...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.3.594

    authors: Goldstein AH,Anderson JO,McDaniel RG

    更新日期:1981-03-01 00:00:00

  • Genetic Regulation of Development in Sorghum bicolor: II. Effect of the ma(3) Allele Mimicked by GA(3).

    abstract::The class III, ma(3) (R)-containing maturity genotypes of Sorghum bicolor (L.) Moench differ from all of the other maturity genotypes in that they flower earlier, exhibit rapid shoot elongation sooner after floral differentiation, and exhibit several morphological differences. In the present study, a class III, ma(3) ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.2.581

    authors: Pao CI,Morgan PW

    更新日期:1986-10-01 00:00:00

  • Phosphorylation of a NAC Transcription Factor by a Calcium/Calmodulin-Dependent Protein Kinase Regulates Abscisic Acid-Induced Antioxidant Defense in Maize.

    abstract::Calcium/calmodulin-dependent protein kinase (CCaMK) has been shown to play an important role in abscisic acid (ABA)-induced antioxidant defense and enhance the tolerance of plants to drought stress. However, its downstream molecular events are poorly understood. Here, we identify a NAC transcription factor, ZmNAC84, i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00168

    authors: Zhu Y,Yan J,Liu W,Liu L,Sheng Y,Sun Y,Li Y,Scheller HV,Jiang M,Hou X,Ni L,Zhang A

    更新日期:2016-07-01 00:00:00

  • l-Phenylalanine Ammonia-lyase (Maize): Partial Purification and Response to Gibberellic Acid and Cycloheximide of l-Phenylalanine and l-Tyrosine Ammonia-lyase Activities.

    abstract::Extracts of maize leaf sheath tissue deaminate both l-phenylalanine and l-tyrosine. The activities with both substrates are enhanced by treating the plant with gibberellic acid. Both activities decrease rapidly at the same rate when tissue is incubated in a moist atmosphere, and this decrease can be slowed by treatmen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.4.480

    authors: Reid PD

    更新日期:1972-10-01 00:00:00

  • The kinetics of in vivo state transitions in mesophyll and guard cell chloroplasts monitored by 77 k fluorescence emission spectra.

    abstract::Fluorescence emission spectral peaks at 685, 695 and 730 nanometers (F685, F695, and F730) were recorded 77 K from diluted leaf tissue and epidermal powders prepared from Saxifraga cernua. The time course for state 1 to state 2 transitions was monitored as changes in the ratios of the three emission peaks. During illu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.4.873

    authors: Mawson BT,Cummins WR

    更新日期:1986-12-01 00:00:00

  • Abundantly and rarely expressed Lhc protein genes exhibit distinct regulation patterns in plants.

    abstract::We have analyzed gene regulation of the Lhc supergene family in poplar (Populus spp.) and Arabidopsis (Arabidopsis thaliana) using digital expression profiling. Multivariate analysis of the tissue-specific, environmental, and developmental Lhc expression patterns in Arabidopsis and poplar was employed to characterize ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.073304

    authors: Klimmek F,Sjödin A,Noutsos C,Leister D,Jansson S

    更新日期:2006-03-01 00:00:00

  • Effect of water stress on the reduction of nitrate and nitrite by soybean nodules.

    abstract::The effects of water stress on nitrate reductase and nitrite reductase activities in symbiotic nodules were examined in field-grown soybean plants (Glycine max L Merr. cv Clark). The in vitro assays of enzyme activity indicated that the nodule cytosol and bacteroids contained both nitrate reductase and nitrite reducta...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.4.1065

    authors: Chen CL,Sung JM

    更新日期:1983-12-01 00:00:00

  • Collection and Identification of Allelopathic Compounds from the Undisturbed Root System of Bigalta Limpograss (Hemarthria altissima).

    abstract::Collection of allelopathic chemicals from the undisturbed plant root system is difficult because of their low concentrations and the high level of contaminants in growth media such as soil. A new approach for the continuous trapping of quantities of extracellular chemicals from donor plants is described. Bigalta limpo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.1.155

    authors: Tang CS,Young CC

    更新日期:1982-01-01 00:00:00

  • Influence of Iron Chlorosis on Pigment and Protein Metabolism in Leaves of Nicotiana tabacum L.

    abstract::Experiments were conducted on Nicotiana tabacum, L. to study the relation in the grana among chlorophylls, carotenoids, and proteins. The effect of iron chlorosis on protein and pigment synthesis was studied at different stages of chlorosis using glycine-U-C(14). Pigments were separated by thin layer chromatography.Ch...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.3.415

    authors: Shetty AS,Miller GW

    更新日期:1966-03-01 00:00:00

  • Alterations in cytosolic glucose-phosphate metabolism affect structural features and biochemical properties of starch-related heteroglycans.

    abstract::The cytosolic pools of glucose-1-phosphate (Glc-1-P) and glucose-6-phosphate are essential intermediates in several biosynthetic paths, including the formation of sucrose and cell wall constituents, and they are also linked to the cytosolic starch-related heteroglycans. In this work, structural features and biochemica...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.127969

    authors: Fettke J,Nunes-Nesi A,Alpers J,Szkop M,Fernie AR,Steup M

    更新日期:2008-11-01 00:00:00

  • Hydroperoxide Lyase and Other Hydroperoxide-Metabolizing Activity in Tissues of Soybean, Glycine max.

    abstract::Hydroperoxide lyase (HPLS) activity in soybean (Glycine max) seed/seedlings, leaves, and chloroplasts of leaves required detergent solubilization for maximum in vitro activity. On a per milligram of protein basis, more HPLS activity was found in leaves, especially chloroplasts, than in seeds or seedlings. The total yi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.3.1059

    authors: Gardner HW,Weisleder D,Plattner RD

    更新日期:1991-11-01 00:00:00

  • A cytosolic Arabidopsis D-xylulose kinase catalyzes the phosphorylation of 1-deoxy-D-xylulose into a precursor of the plastidial isoprenoid pathway.

    abstract::Plants are able to integrate exogenous 1-deoxy-D-xylulose (DX) into the 2C-methyl-D-erythritol 4-phosphate pathway, implicated in the biosynthesis of plastidial isoprenoids. Thus, the carbohydrate needs to be phosphorylated into 1-deoxy-D-xylulose 5-phosphate and translocated into plastids, or vice versa. An enzyme ca...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.086652

    authors: Hemmerlin A,Tritsch D,Hartmann M,Pacaud K,Hoeffler JF,van Dorsselaer A,Rohmer M,Bach TJ

    更新日期:2006-10-01 00:00:00

  • Characterization of Plasma Membrane-associated Adenosine Triphosphase Activity of Oat Roots.

    abstract::ATPase activity of plasma membranes isolated from oat (Avena sativa L. cv. Goodfield) roots was activated by divalent cations (Mg(2+) = Mn(2+) > Zn(2+) > Fe(2+) > Ca(2+)) and further stimulated by KCl and a variety of monovalent salts, both inorganic and organic. The enzyme exhibited greater specificity for cations th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.1.6

    authors: Leonard RT,Hodges TK

    更新日期:1973-07-01 00:00:00

  • Critical Roles of Vacuolar Invertase in Floral Organ Development and Male and Female Fertilities Are Revealed through Characterization of GhVIN1-RNAi Cotton Plants.

    abstract::Seed number and quality are key traits determining plant fitness and crop yield and rely on combined competence in male and female fertilities. Sucrose metabolism is central to reproductive success. It remains elusive, though, how individual sucrose metabolic enzymes may regulate the complex reproductive processes. He...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00197

    authors: Wang L,Ruan YL

    更新日期:2016-05-01 00:00:00