Abstract:
:We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Zhang C,Barthelson RA,Lambert GM,Galbraith DWdoi
10.1104/pp.107.115246subject
Has Abstractpub_date
2008-05-01 00:00:00pages
30-40issue
1eissn
0032-0889issn
1532-2548pii
pp.107.115246journal_volume
147pub_type
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