Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei.

Abstract:

:We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Zhang C,Barthelson RA,Lambert GM,Galbraith DW

doi

10.1104/pp.107.115246

subject

Has Abstract

pub_date

2008-05-01 00:00:00

pages

30-40

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.107.115246

journal_volume

147

pub_type

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