Abstract:
:The influence of polyploidization on chloroplast coupling factor 1 (CF1) was examined in leaves and leaf protoplasts from isogenic diploid-tetraploid (DDC 2X-4X) and tetraploid-octoploid (IC 4X-8X) sets of alfalfa (Medicago sativa L.). Alfalfa CF1 was purified to homogeneity and was found to contain five subunits with molecular weights of 57,900, 54,300, 38,700, 23,100, and 15,200. Rocket immunoelectrophoresis with anti-spinach CF1 gamma immunoglobulins was used to quantify CF1 from protoplasts and leaves. In the DDC 2X-4X set, fresh weight per cell and cellular content of CF1, chlorophyll (Chl), and DNA doubled with ploidy. Ratios of CF1 to Chl of leaf protoplasts and leaves were similar between diploids and tetraploids. In the IC 4X-8X set, octoploid protoplasts were 90% higher in Chl than were comparable tetraploids, whereas octoploids were 50 to 60% higher than tetraploids in fresh weight per cell and cellular content of CF1 and DNA. Concentrations of CF1 and Chl in leaves and ratios of CF1 to DNA in protoplasts were similar across ploidy levels of both isogenic sets. Therefore, cellular content of CF1 increases proportionately with the amount of DNA per cell or gene dosage.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Meyers SP,Molin WT,Selman BR,Schrader LEdoi
10.1104/pp.70.6.1715subject
Has Abstractpub_date
1982-12-01 00:00:00pages
1715-7issue
6eissn
0032-0889issn
1532-2548journal_volume
70pub_type
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