Cysteine transport into cultured tobacco cells.

Abstract:

:Cysteine transport by tobacco cells (Nicotiana tabacum L. var. Xanthi) cultured on liquid B-5 medium was examined.Transport was linear with time or amount of tissue and had a pH optimum of 4.5. Cysteine transport over a wide concentration range was biphasic. The isotherm, for descriptive convenience, was divided into two segments both of which obeyed Michaelis-Menten kinetics. The Km for high affinity transport was in the range 1.7 x 10(-5)m(+/-0.17) while the Km for low affinity transport was in the range 3.5 x 10(-4)m(+/-0.13). Maximum velocities were 3 to 6 nmoles/g fresh weight/minute and 13 to 16 nmoles/g fresh weight/minute, respectively.Azide and 2,4-dinitrophenol caused more than 90% inhibition of net transport by either system. N,N'-Dicyclohexylcarbodiimide was not inhibitory while the inhibition by carbonylcyanide m-chlorophenylhydrazone was dependent on the cysteine concentration. Only those compounds that were inhibitory to transport caused significant efflux of labeled material from preloaded cells.Tobacco cells that had been preincubated in iodoacetamide or N-ethylmaleimide did not transport cysteine while similar treatments with dithiothreitol were only slightly inhibitory or had no effect on transport.Transport by either system was, to some extent, inhibited by all other tested amino acids and analogs. Alanine, methionine, and S-methyl cysteine were most effective in inhibiting cysteine transport. Both alanine and methionine were competitive inhibitors of cysteine transport by either system with inhibition constants that were similar to the Km for the particular system.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Harrington HM,Smith IK

doi

10.1104/pp.60.6.807

subject

Has Abstract

pub_date

1977-12-01 00:00:00

pages

807-11

issue

6

eissn

0032-0889

issn

1532-2548

journal_volume

60

pub_type

杂志文章
  • Metabolism of Indole-3-Acetic Acid: III. Identification of Metabolites Isolated from Crown Gall Callus Tissue.

    abstract::The metabolism of labeled indole-3-acetic acid (IAA-2-(14)C) was investigated in Parthenocissus tricuspidata crown gall callus tissue. After 48 hours incubation, 85 to 90% of the supplied IAA was taken up by the tissue, and of that taken up, about 45% was conjugated with five amino acids. The conjugates found were asp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.5.666

    authors: Feung CS,Hamilton RH,Mumma RO

    更新日期:1976-11-01 00:00:00

  • Anaerobic Formation of d-Lactate and Partial Purification and Characterization of a Pyruvate Reductase from Chlamydomonas reinhardtii.

    abstract::d-Lactate accumulation in Chlamydomonas reinhardtii was dependent on anaerobic conditions. As much as 50% of the (14)C after 2 minutes of photosynthetic (14)CO(2) fixation moved into d-lactate from sugar phosphates if the cells became anaerobic for short time periods. No lactate accumulated in the dark until the O(2) ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.2.277

    authors: Husic DW,Tolbert NE

    更新日期:1985-06-01 00:00:00

  • The mechanic state of "inner tissue" in the growing zone of sunflower hypocotyls and the regulation of its growth rate following excision.

    abstract::Spontaneous growth of isolated inner tissue from the etiolated sunflower (Helianthus annuus L.) hypocotyl growing zone was investigated. A new preparation technique allowed measurements starting 3 s after excision. Elongation with respect to the turgescent and plasmolized state was quantified in terms of relative grow...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.123.2.605

    authors: Peters WS,Tomos AD

    更新日期:2000-06-01 00:00:00

  • Localization of [gamma]-Glutamylcysteine Synthetase and Glutathione Synthetase Activity in Maize Seedlings.

    abstract::Fresh weight, protein, cysteine, [gamma]-glutamylcysteine, glutathione, and the extractable activity of the enzymes of glutathione biosynthesis, [gamma]-glutamylcysteine synthetase (EC 6.3.2.2) and glutathione synthetase (EC 6.3.2.3), were measured in roots, scutella, endosperms, and shoots of 3-, 7-, and 11-d-old mai...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.2.561

    authors: Ruegsegger A,Brunold C

    更新日期:1993-02-01 00:00:00

  • Estimation of Bundle Sheath Cell Conductance in C4 Species and O2 Insensitivity of Photosynthesis.

    abstract::Low conductance to CO2 of bundle sheath cells is required in C4 photosynthesis to maintain high [CO2] at the site of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Elevated [CO2] allows high CO2 assimilation rates by this enzyme and prevents Rubisco oxygenase activity and O2 inhibition of carboxylation. Bu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.4.1183

    authors: Brown RH,Byrd GT

    更新日期:1993-12-01 00:00:00

  • Alteration of Cell-Wall Water Content and Elasticity in Douglas-Fir during Periods of Water Deficit.

    abstract::Three populations of seedlings of Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) were subjected to recurrent drought cycles. Pressure-volume curves were developed to derive the osmotic and pressure potentials as functions of the symplastic water over a wide range of relative water content. The drought cycles induc...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.2.418

    authors: Joly RJ,Zaerr JB

    更新日期:1987-02-01 00:00:00

  • Cyclopropane Fatty acids in relation to earliness in spring and drought tolerance in plants.

    abstract::Long chain cyclopropane fatty acids were observed in the sulfolipid fraction extracted from leaves of the early spring plants Galanthus nivalis L. and Anthriscus silvestris L. (Hoffm.). The content of cyclopropane fatty acids with 25 carbon atoms appeared to be clearly correlated with earliness in spring, and it range...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.3.307

    authors: Kuiper PJ,Stuiver B

    更新日期:1972-03-01 00:00:00

  • NaJAZh regulates a subset of defense responses against herbivores and spontaneous leaf necrosis in Nicotiana attenuata plants.

    abstract::The JASMONATE ZIM DOMAIN (JAZ) proteins function as negative regulators of jasmonic acid signaling in plants. We cloned 12 JAZ genes from native tobacco (Nicotiana attenuata), including nine novel JAZs in tobacco, and examined their expression in plants that had leaves elicited by wounding or simulated herbivory. Most...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.193771

    authors: Oh Y,Baldwin IT,Gális I

    更新日期:2012-06-01 00:00:00

  • Acclimation of Photosynthetic Light Reactions during Induction of Inorganic Carbon Accumulation in the Green Alga Chlamydomonas reinhardtii.

    abstract::Cells of the unicellular green algae Chlamydomonas reinhardtii were grown in high dissolved inorganic carbon (DIC) concentrations (supplied with 50 milliliters per liter CO(2)[g]) and transferred to low DIC concentrations (supplied with

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.1.357

    authors: Palmqvist K,Sundblad LG,Wingsle G,Samuelsson G

    更新日期:1990-09-01 00:00:00

  • Signal peptide-dependent targeting of a rice alpha-amylase and cargo proteins to plastids and extracellular compartments of plant cells.

    abstract::alpha-Amylases are important enzymes for starch degradation in plants. However, it has been a long-running debate as to whether alpha-amylases are localized in plastids where starch is stored. To study the subcellular localization of alpha-amylases in plant cells, a rice (Oryza sativa) alpha-amylase, alphaAmy3, with o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.042184

    authors: Chen MH,Huang LF,Li HM,Chen YR,Yu SM

    更新日期:2004-07-01 00:00:00

  • De novo synthesis of 3'-nucleotidase in germinating wheat embryo.

    abstract::The enzyme 3'-AMP nucleotidase was purified 2,500- to 5,000-fold from extracts of an acetone powder of wheat (Triticum aestivum) embryonic axes germinated for 40 hours. Sodium dodecyl sulfate acrylamide gel electrophoresis and chromatography on Biogel-P100 indicate that the enzyme is monomeric with a molecular weight ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.2.295

    authors: Zhu ZP,Marsh L,Marcus A

    更新日期:1983-02-01 00:00:00

  • Starch-synthesizing Enzymes in the Endosperm and Pollen of Maize.

    abstract::Two mutations, amylose-extender and waxy, which affect the proportion of amylose and amylopectin of starch synthesized in the endosperm of maize (Zea mays L.) seeds, are also expressed in the pollen. However, most mutations that affect starch synthesis in the maize endosperm are not expressed in the pollen. In an atte...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.2.312

    authors: Bryce WH,Nelson OE

    更新日期:1979-02-01 00:00:00

  • Early effects of salinity on nitrate assimilation in barley seedlings.

    abstract::The effect of NaCl and Na(2)SO(4) salinity on NO(3) (-) assimilation in young barley (Hordeum vulgare L. var Numar) seedlings was studied. The induction of the NO(3) (-) transporter was affected very little; the major effect of the salts was on its activity. Both Cl(-) and SO(4) (2-) salts severely inhibited uptake of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.2.321

    authors: Aslam M,Huffaker RC,Rains DW

    更新日期:1984-10-01 00:00:00

  • Fructose 2,6-bisphosphate inhibition of phosphoglucomutase.

    abstract::Fructose 2,6-bisphosphate inhibits phosphoglucomutase noncompetitively with respect to the cofactor glucose 1,6-bisphosphate. Previous studies from our laboratory had shown that phosphoglucomutase was activated by fructose 2,6-bisphosphate in the absence of added glucose 1,6-bisphosphate. The fructose 2,6-bisphosphate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.4.980

    authors: Galloway CM,Dugger WM,Black CC

    更新日期:1988-12-01 00:00:00

  • Evaluation of h secretion relative to zeatin-induced growth of detached cucumber cotyledons.

    abstract::Cytokinins promote expansion of cotyledons detached from seedlings of more than a dozen species. The zeatin-enhanced expansion of cucumber (Cucumis sativus L. cv Marketer) cotyledons was investigated. In addition, whether acid secretion is involved in wall loosening accompanying such accelerated growth was evaluated. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.5.1470

    authors: Ross CW,Rayle DL

    更新日期:1982-11-01 00:00:00

  • Decreased Exopolysaccharide Synthesis by Anaerobic and Symbiotic Cells of Bradyrhizobium japonicum.

    abstract::Experiments were conducted to determine whether symbiotic bacteroids of Bradyrhizobium japonicum produce exopolysaccharide within soybean (Glycine max [L.] Merr. cv ;Lee 74') nodules. B. japonicum strains RT2, a derivative of USDA 110 with resistance to streptomycin and rifampicin, and RT176-1, a mutant deficient in e...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.2.445

    authors: Tully RE,Terry ME

    更新日期:1985-10-01 00:00:00

  • Metabolism of Gibberellin A(12) and A(12)-Aldehyde in Developing Seeds of Pisum sativum L.

    abstract::Metabolism of [(14)C]gibberellin (GA) A(12) (GA(12)) and [(14)C]gibberellin A(12)-aldehyde (GA(12)-aldehyde) was examined in cotyledons and seed coats from developing seeds of pea (Pisum sativum L.). Both were metabolized to only 13-hydroxylated GAs in cotyledons but to 13-hydroxylated and non-13-hydroxylated GAs in s...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.1.26

    authors: Zhu YX,Davies PJ,Halinska A

    更新日期:1991-09-01 00:00:00

  • Long-Distance Water Transport in Aquatic Plants.

    abstract::Acropetal mass flow of water is demonstrated in two submerged angiosperms, Lobelia dortmanna L. and Sparganium emersum Rehman by means of guttation measurements. Transpiration is absent in truly submerged plants, but the presence of guttation verifies that long-distance water transport takes place. Use of tritiated wa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.4.1369

    authors: Pedersen O

    更新日期:1993-12-01 00:00:00

  • Effect of Glucose and CO(2) on Nitrate Uptake and Coupled OH Flux in Ankistrodesmus braunii.

    abstract::In Ankistrodesmus braunii, in the absence of CO(2), i.e. in CO(2)-free air or N(2), photosynthetic nitrate uptake and nitrate reduction were inhibited, especially at low pH. Under such conditions, glucose stimulated nitrate uptake and reduction to almost the same level in the pH range between 6 and 8.5. CO(2) at 0.03%...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.1.18

    authors: Eisele R,Ullrich WR

    更新日期:1977-01-01 00:00:00

  • Pentose phosphate metabolism of potato tuber discs as influenced by prior storage temperature.

    abstract::Tubers of Solanum tuberosum L. var. Russet Burbank, Kennebec, and Targhee were stored at 95% relative humidity and at 1.7, 4.4, 5.8, 7.2 or 10 C. Rates of O(2) consumption were measured periodically by removing individual tubers from storage, excising discs of pith parenchyma tissue, and incubating the discs in a Gils...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.2.252

    authors: Dwelle RB,Stallknecht GF

    更新日期:1978-02-01 00:00:00

  • Plasma membrane H+-ATPase is involved in auxin-mediated cell elongation during wheat embryo development.

    abstract::Previous investigations suggested that specific auxin spatial distribution due to auxin movements to particular embryonic regions was important for normal embryonic pattern formation. To gain information on the molecular mechanism(s) by which auxin acts to direct pattern formation in specific embryonic regions, the ro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.013466

    authors: Rober-Kleber N,Albrechtová JT,Fleig S,Huck N,Michalke W,Wagner E,Speth V,Neuhaus G,Fischer-Iglesias C

    更新日期:2003-03-01 00:00:00

  • Differential Ion Stimulation of Plasmalemma Adenosine Triphosphatase from Leaf Epidermis and Mesophyll of Nicotiana rustica L.

    abstract::An ATPase preparation, presumed to be associated with plasma membrane due to the coincidence on isopycnic gradients of cellulase and beta-glucan synthetase at high substrate, has been isolated from the epidermal and mesophyll of tobacco leaf. The ATPase from both tissues was found to prefer ATP over other nucleotides....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.5.936

    authors: Lurie S

    更新日期:1979-05-01 00:00:00

  • Influence of Anaerobiosis on Chlorophyll Biosynthesis in Greening Oat Seedlings (Avena sativa L.).

    abstract::The influence of anaerobiosis for 0.5 to 15 hours on the last steps of chlorophyll biosynthesis of etiolated oat seedlings was investigated. Phototransformation of protochlorophyllide to chlorophyllide is only slightly reduced and esterification of chlorophyllide is slightly increased by pretreatment under anaerobic c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.4.576

    authors: Schoch S,Hehlein C,Rüdiger W

    更新日期:1980-10-01 00:00:00

  • The multiple roles of conserved arginine 286 of 1-aminocyclopropane-1-carboxylate synthase. Coenzyme binding, substrate binding, and beyond.

    abstract::A pyridoxal 5'-phosphate (PLP)-dependent enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (S-adenosyl-L-Met methylthioadenosine-lyase, EC 4.4.1.14), catalyzes the conversion of S-adenosyl-L-methionine (AdoMet) to ACC. A tomato ACC synthase isozyme (LE-ACS2) with a deletion of 46 amino acids at the C termin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.121.3.913

    authors: Zhou H,Wang HW,Zhu K,Sui SF,Xu P,Yang SF,Li N

    更新日期:1999-11-01 00:00:00

  • Histochemical Compartmentation of Photosynthesis in the Crassulacean Acid Metabolism Plant Crassula falcata.

    abstract::The succulent leaf of the obligate Crassulacean acid metabolism plant Crassula falcata comprises two distinct types of parenchyma. The peripheral tissue is dark green, whereas the central tissue is relatively colorless. We have investigated whether the conventional interpretation of Crassulacean acid metabolism-simply...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.3.633

    authors: Springer SA,Outlaw WH

    更新日期:1988-11-01 00:00:00

  • Enzymes of starch metabolism in the developing rice grain.

    abstract::The levels of starch, soluble sugars, protein, and enzymes involved in starch metabolism-alpha-amylase, beta-amylase, phosphorylase, Q-enzyme, R-enzyme, and starch synthetase -were assayed in dehulled developing rice grains (Oryzasativa L., variety IR8). Phosphorylase, Q-enzyme, and R-enzyme had peak activities 10 day...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.3.429

    authors: Baun LC,Palmiano EP,Perez CM,Juliano BO

    更新日期:1970-09-01 00:00:00

  • Mitochondrial ABC Transporter ATM3 Is Essential for Cytosolic Iron-Sulfur Cluster Assembly.

    abstract::The mitochondrial ATP-binding cassette transporter ATM3 has been studied in Arabidopsis. Its function, however, is poorly understood in other model plant species. This study reports that the ATM3 is required for cytosolic iron-sulfur cluster assembly and is essential for meristem maintenance in rice (Oryza sativa). Th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01760

    authors: Zuo J,Wu Z,Li Y,Shen Z,Feng X,Zhang M,Ye H

    更新日期:2017-04-01 00:00:00

  • SIPP, a Novel Mitochondrial Phosphate Carrier, Mediates in Self-Incompatibility.

    abstract::In Solanaceae, the S-specific interaction between the pistil S-RNase and the pollen S-Locus F-box protein controls self-incompatibility (SI). Although this interaction defines the specificity of the pollen rejection response, the identification of three pistil essential modifier genes unlinked to the S-locus (HT-B, 12...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01884

    authors: García-Valencia LE,Bravo-Alberto CE,Wu HM,Rodríguez-Sotres R,Cheung AY,Cruz-García F

    更新日期:2017-11-01 00:00:00

  • Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes.

    abstract::The Dof (DNA binding with one finger) transcriptional activator rice (Oryza sativa) prolamin box binding factor (RPBF), which is involved in gene regulation of rice seed storage proteins, has been isolated from rice cDNA expressed sequence tag clones containing the conserved Dof. RPBF is found as a single gene per hap...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.082826

    authors: Yamamoto MP,Onodera Y,Touno SM,Takaiwa F

    更新日期:2006-08-01 00:00:00

  • Evidence for nuclear processing of plant micro RNA and short interfering RNA precursors.

    abstract::The Arabidopsis genome encodes four Dicer-like (DCL) proteins, two of which contain putative nuclear localization signals. This suggests one or more nuclear pathways for processing double-stranded (ds) RNA in plants. To study the subcellular location of processing of nuclear-encoded dsRNA involved in transcriptional s...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.021980

    authors: Papp I,Mette MF,Aufsatz W,Daxinger L,Schauer SE,Ray A,van der Winden J,Matzke M,Matzke AJ

    更新日期:2003-07-01 00:00:00