The multiple roles of conserved arginine 286 of 1-aminocyclopropane-1-carboxylate synthase. Coenzyme binding, substrate binding, and beyond.

Abstract:

:A pyridoxal 5'-phosphate (PLP)-dependent enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (S-adenosyl-L-Met methylthioadenosine-lyase, EC 4.4.1.14), catalyzes the conversion of S-adenosyl-L-methionine (AdoMet) to ACC. A tomato ACC synthase isozyme (LE-ACS2) with a deletion of 46 amino acids at the C terminus was chosen as the control enzyme for the study of the function of R286 in ACC synthase. R286 of the tomato ACC synthase was mutated to a leucine via site-directed mutagenesis. The ACC synthase mutant R286L was purified using a simplified two-step purification protocol. Circular dichroism (CD) analysis indicated that the overall three-dimensional structure of the mutant was indistinguishable from that of the control enzyme. Fluorescence spectroscopy revealed that the binding affinity of R286L ACC synthase for its cofactor PLP was reduced 20- to 25-fold compared with control. Kinetic analysis of R286L showed that this mutant ACC synthase had a significantly reduced turnover number (k(cat)) of 8.2 x 10(-3) s(-1) and an increased K(m) of 730 microM for AdoMet, leading to an 8,000-fold decrease in overall catalytic efficiency compared with the control enzyme. Thus, R286 of tomato ACC synthase is involved in binding both PLP and AdoMet.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Zhou H,Wang HW,Zhu K,Sui SF,Xu P,Yang SF,Li N

doi

10.1104/pp.121.3.913

subject

Has Abstract

pub_date

1999-11-01 00:00:00

pages

913-9

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

121

pub_type

杂志文章
  • Structure and function of developing barley plastids.

    abstract::Five different regions of the first foliage leaf of etiolated barley seedlings were studied with respect to leaf growth, plastid growth and replication, differentiation of etioplasts, and conversion of etioplasts into chloroplasts upon illumination. Ultrastructural changes of the plastids were correlated with chloroph...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.2.148

    authors: Robertson D,Laetsch WM

    更新日期:1974-08-01 00:00:00

  • Ca transport in membrane vesicles from pinto bean leaves and its alteration after ozone exposure.

    abstract::The influence of ozone on Ca(2+) transport in plant membranes from pinto bean (Phaseolus vulgaris L. var Pinto) leaves was investigated in vitro by means of a filtration method using purified vesicles. Two transport mechanisms located at the plasma membrane are involved in a response to ozone: (a) passive Ca(2+) influ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.2.788

    authors: Castillo FJ,Heath RL

    更新日期:1990-10-01 00:00:00

  • Enzymic Dissociation of Zea Shoot Cell Wall Polysaccharides : IV. Dissociation of Glucuronoarabinoxylan by Purified Endo-(1 --> 4)-beta-Xylanase from Bacillus subtilis.

    abstract::The structure of a glucuronoarabinoxylan in Zea mays L. (hybrid B73 x Mo17) shoot cell walls has been studied. The water-insoluble fraction of Zea shoot cell walls, pretreated with purified Bacillus subtilis (1 --> 3), (1 --> 4)-beta-d-glucan 4-glucanohydrolase, was treated with purified B. subtilis endo-(1 --> 4)-bet...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.3.759

    authors: Kato Y,Nevins DJ

    更新日期:1984-07-01 00:00:00

  • Characterization of a maize tonoplast aquaporin expressed in zones of cell division and elongation.

    abstract::We studied aquaporins in maize (Zea mays), an important crop in which numerous studies on plant water relations have been carried out. A maize cDNA, ZmTIP1, was isolated by reverse transcription-coupled PCR using conserved motifs from plant aquaporins. The derived amino acid sequence of ZmTIP1 shows 76% sequence ident...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.4.1143

    authors: Chaumont F,Barrieu F,Herman EM,Chrispeels MJ

    更新日期:1998-08-01 00:00:00

  • Characterization of FRO1, a pea ferric-chelate reductase involved in root iron acquisition.

    abstract::To acquire iron, many plant species reduce soil Fe(III) to Fe(II) by Fe(III)-chelate reductases embedded in the plasma membrane of root epidermal cells. The reduced product is then taken up by Fe(II) transporter proteins. These activities are induced under Fe deficiency. We describe here the FRO1 gene from pea (Pisum ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010829

    authors: Waters BM,Blevins DG,Eide DJ

    更新日期:2002-05-01 00:00:00

  • The purple cauliflower arises from activation of a MYB transcription factor.

    abstract::Anthocyanins are responsible for the color of many flowers, fruits, and vegetables. An interesting and unique Purple (Pr) gene mutation in cauliflower (Brassica oleracea var botrytis) confers an abnormal pattern of anthocyanin accumulation, giving the striking mutant phenotype of intense purple color in curds and a fe...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.164160

    authors: Chiu LW,Zhou X,Burke S,Wu X,Prior RL,Li L

    更新日期:2010-11-01 00:00:00

  • Cold storage of isolated class C chloroplasts: optimal conditions for stabilization of photosynthetic activities.

    abstract::Preservation of photosynthetic activities (photophosphorylation, electron transport, fluorescence induction, 0.3-second delayed light emission) of isolated broken (class C) chloroplasts by low temperature storage was investigated under a wide range of conditions in order to optimize long time activity retention.The mo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.6.942

    authors: Farkas DL,Malkin S

    更新日期:1979-12-01 00:00:00

  • Colocalization of plastid division proteins in the chloroplast stromal compartment establishes a new functional relationship between FtsZ1 and FtsZ2 in higher plants.

    abstract::Chloroplast division is driven by a macromolecular complex containing components that are positioned on the cytosolic surface of the outer envelope, the stromal surface of the inner envelope, and in the intermembrane space. The only constituents of the division apparatus identified thus far are the tubulin-like protei...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: McAndrew RS,Froehlich JE,Vitha S,Stokes KD,Osteryoung KW

    更新日期:2001-12-01 00:00:00

  • Altered gene expression in three plant species in response to treatment with Nep1, a fungal protein that causes necrosis.

    abstract::Nep1 is an extracellular fungal protein that causes necrosis when applied to many dicotyledonous plants, including invasive weed species. Using transmission electron microscopy, it was determined that application of Nep1 (1.0 micro g mL(-)(1), 0.1% [v/v] Silwet-L77) to Arabidopsis and two invasive weed species, spotte...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.019836

    authors: Keates SE,Kostman TA,Anderson JD,Bailey BA

    更新日期:2003-07-01 00:00:00

  • Regulation of alternative oxidase activity in six wild monocotyledonous species. An in vivo study at the whole root level.

    abstract::The activity of the alternative pathway is affected by a number of factors, including the level and reduction state of the alternative oxidase (AOX) protein, and the reduction state of the ubiquinone pool. To investigate the significance of these factors for the rate of alternative respiration in vivo, we studied root...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.1.376

    authors: Millenaar FF,Gonzàlez-Meler MA,Fiorani F,Welschen R,Ribas-Carbo M,Siedow JN,Wagner AM,Lambers H

    更新日期:2001-05-01 00:00:00

  • Reduced Photosystem II Activity and Accumulation of Viral Coat Protein in Chloroplasts of Leaves Infected with Tobacco Mosaic Virus.

    abstract::We previously reported (A Reinero, RN Beachy 1986 Plant Mol Biol 6:291-301) that coat protein (CP) of tobacco mosaic virus (TMV) accumulates in chloroplasts of systemically infected leaves. To determine the significance of such interaction we examined electron transport rates in chloroplasts containing different level...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.1.111

    authors: Reinero A,Beachy RN

    更新日期:1989-01-01 00:00:00

  • Intermediate reactions of oxidative phosphorylation in mitochondria from cabbage.

    abstract::Respiratory control ratios between 2.0 and 9.0 were obtained by comparison of the respiratory rates of cabbage mitochondria in the presence and in the absence of individual components of the system used to provide ADP and by comparing the rates before and after exhaustion of added ADP. These results indicate that resp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.3.327

    authors: Lotlikar PD,Remmert LF

    更新日期:1968-03-01 00:00:00

  • Characterization of a Giant PSI Supercomplex in the Symbiotic Dinoflagellate Symbiodiniaceae.

    abstract::Symbiodiniaceae are symbiotic dinoflagellates that provide photosynthetic products to corals. Because corals are distributed across a wide range of depths in the ocean, Symbiodiniaceae species must adapt to various light environments to optimize their photosynthetic performance. However, as few biochemical studies of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00726

    authors: Kato H,Tokutsu R,Kubota-Kawai H,Burton-Smith RN,Kim E,Minagawa J

    更新日期:2020-08-01 00:00:00

  • Beta-subunits of the SnRK1 complexes share a common ancestral function together with expression and function specificities; physical interaction with nitrate reductase specifically occurs via AKINbeta1-subunit.

    abstract::The SNF1/AMPK/SnRK1 kinases are evolutionary conserved kinases involved in yeast, mammals, and plants in the control of energy balance. These heterotrimeric enzymes are composed of one alpha-type catalytic subunit and two gamma- and beta-type regulatory subunits. In yeast it has been proposed that the beta-type subuni...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.123026

    authors: Polge C,Jossier M,Crozet P,Gissot L,Thomas M

    更新日期:2008-11-01 00:00:00

  • Effects of vanadate on the plasma membrane ATPase of red beet and corn.

    abstract::The effect of vanadate on the plant plasma membrane ATPase were investigated in plasma membrane fractions derived from corn roots (Zea mays L.) and red beets (Beta vulgaris L.). The K(i) for vanadate inhibition of the plasma membrane ATPase from corn roots and red beets was between 6 and 15 micromolar vanadate. In bot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.3.586

    authors: O'neill SD,Spanswick RM

    更新日期:1984-07-01 00:00:00

  • Chemical composition of the Prunus laurocerasus leaf surface. Dynamic changes of the epicuticular wax film during leaf development.

    abstract::The seasonal development of adaxial Prunus laurocerasus leaf surfaces was studied using newly developed methods for the mechanical removal of epicuticular waxes. During epidermal cell expansion, more than 50 microg leaf(-1) of alkyl acetates accumulated within 10 d, forming an epicuticular wax film approximately 30 nm...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.4.1725

    authors: Jetter R,Schäffer S

    更新日期:2001-08-01 00:00:00

  • Phosphatidate Kinase, a Novel Enzyme in Phospholipid Metabolism (Purification, Subcellular Localization, and Occurrence in the Plant Kingdom).

    abstract::Microsomal membranes from suspension-cultured Catharanthus roseus cells possess an enzymic activity that catalyzes the ATP-dependent phosphorylation of phosphatidic acid (PA) to form diacylglycerol pyrophosphate (H. Behrbohm, J.B. Wissing [1993] FEBS Lett 315: 95-99). This enzyme activity, PA kinase, was purified and ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.4.1243

    authors: Wissing JB,Behrbohm H

    更新日期:1993-08-01 00:00:00

  • Identification of a specific isoform of tomato lipoxygenase (TomloxC) involved in the generation of fatty acid-derived flavor compounds.

    abstract::There are at least five lipoxygenases (TomloxA, TomloxB, TomloxC, TomloxD, and TomloxE) present in tomato (Lycopersicon esculentum Mill.) fruit, but their role in generation of fruit flavor volatiles has been unclear. To assess the physiological role of TomloxC in the generation of volatile C6 aldehyde and alcohol fla...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.041608

    authors: Chen G,Hackett R,Walker D,Taylor A,Lin Z,Grierson D

    更新日期:2004-09-01 00:00:00

  • Chilling-Induced Lipid Degradation in Cucumber (Cucumis sativa L. cv Hybrid C) Fruit.

    abstract::Chilling at 4 degrees C in the dark induced lipid degradation in cucumber (Cucumis sativa L.) fruit upon rewarming at 14 degrees C. Rates of ethane evolution by fruits rewarmed after 3 days of chilling were up to four-fold higher than those evolved by unchilled (14 degrees C) fruits (0.02-0.05 picomoles gram fresh wei...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.3.1049

    authors: Parkin KL,Kuo SJ

    更新日期:1989-07-01 00:00:00

  • The effect of 1,4-dihydropyridines on the initiation and development of gametophore buds in the moss funaria.

    abstract::The plant hormone cytokinin stimulates target caulonemata of Funaria to form buds that develop into the leafy gametophyte. Previous reports have shown that increases in intracellular Ca(2+) occur during hormone-activated budding concomitant with an alteration in the polarity of the organelles in the bud site. In order...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.3.684

    authors: Conrad PA,Hepler PK

    更新日期:1988-03-01 00:00:00

  • Thermal conductivity of functional citrus tree wood.

    abstract::Thermal conductivity coefficients have been determined for longitudinal and transverse flow in 4 varieties of fresh Citrus wood using steady state-methods. Equations were developed from which thermal conductivity could be rapidly estimated from moisture content or electrical conductivity. The heat balance of large and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.8.1025

    authors: Turrell FM,Austin SW,McNee D,Park WJ

    更新日期:1967-08-01 00:00:00

  • Biochemical Characterization of an Acetylcholine-hydrolyzing Enzyme from Bean Seedlings.

    abstract::An acetylcholine hydrolyzing enzyme was prepared and purified (40 times) from dwarf bean hypocotyl hooks. The purity of the enzyme was proved by polyacrylamide gel electrophoresis. The molecular weight of the enzyme was determined to be 65,000 daltons. Enzyme activity was the highest at pH 8.0 and between 30 and 36 C....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.3.447

    authors: Ernst M,Hartmann E

    更新日期:1980-03-01 00:00:00

  • NPR genes, which are negatively regulated by phytochrome action in Lemna gibba L. G-3, can also be positively regulated by abscisic acid.

    abstract::We have found that NPR1 and NPR2, two genes from Lemna gibba L. G-3 that can be negatively regulated by phytochrome action, can also be positively regulated by the plant hormone abscisic acid (ABA). Both genes were responsive to low concentrations of exogenous ABA; an increase in NPR1 RNA could be detected in response...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.3.949

    authors: Williams SA,Weatherwax SC,Bray EA,Tobin EM

    更新日期:1994-07-01 00:00:00

  • Osmotic adjustment, symplast volume, and nonstomatally mediated water stress inhibition of photosynthesis in wheat.

    abstract::At low water potential (psi(w)), dehydration reduces the symplast volume of leaf tissue. The effect of this reduction on photosynthetic capacity was investigated. The influence of osmotic adjustment on this relationship was also examined. To examine these relationships, comparative studies were undertaken on two wheat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.4.1040

    authors: Gupta AS,Berkowitz GA

    更新日期:1987-12-01 00:00:00

  • Unique drought resistance functions of the highly ABA-induced clade A protein phosphatase 2Cs.

    abstract::Six Arabidopsis (Arabidopsis thaliana) clade A protein phosphatase 2Cs (PP2Cs) have established abscisic acid (ABA) signaling roles; however, phenotypic roles of the remaining three "HAI" PP2Cs, Highly ABA-Induced1 (HAI1), AKT1-Interacting PP2C1/HAI2, and HAI3, have remained unclear. HAI PP2C mutants had enhanced prol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.202408

    authors: Bhaskara GB,Nguyen TT,Verslues PE

    更新日期:2012-09-01 00:00:00

  • Purification of the Major Soybean Leaf Acid Phosphatase That Is Increased by Seed-Pod Removal.

    abstract::Fruit removal for 5 weeks after flowering increased acid phosphatase activity 10-fold in soybean (Glycine max L. Merr. Var Hobbit) leaves compared with normal seed-pod-bearing plants. The major acid phosphatase activity in leaves was purified over 2700-fold, yielding a single polypeptide of 51 kD with a specific activ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.1.49

    authors: Staswick PE,Papa C,Huang JF,Rhee Y

    更新日期:1994-01-01 00:00:00

  • Fourier transform infrared microspectroscopy detects changes in protein secondary structure associated with desiccation tolerance in developing maize embryos

    abstract::Isolated immature maize (Zea mays L.) embryos have been shown to acquire tolerance to rapid drying between 22 and 25 d after pollination (DAP) and to slow drying from 18 DAP onward. To investigate adaptations in protein profile in association with the acquisition of desiccation tolerance in isolated, immature maize em...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.3.1169

    authors: Wolkers WF,Bochicchio A,Selvaggi G,Hoekstra FA

    更新日期:1998-03-01 00:00:00

  • Cytokinins extracted from pinto bean fruit.

    abstract::Extracts from various parts of Phaseolus vulgaris L. var. Pinto plants were found to exhibit cytokinin activity with the highest levels present in extracts of the fruit tissue. To separate the cytokinins present in the fruit, extracts were chromatographed in several solvent systems. Bioassays of chromatograms indicate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.48.3.320

    authors: Krasnuk M,Witham FH,Tegley JR

    更新日期:1971-09-01 00:00:00

  • Reversal of hydroxyproline-induced inhibition of elongation of Avena coleoptiles.

    abstract::Hydroxy-l-proline-induced inhibition of elongation of Avena coleoptile segments was measured in water and in indole-3-acetic acid. This inhibition was completely reversed by l-proline.Time-sequence experiments revealed that some time had to elapse, or some elongation had to occur, prior to the onset of hydroxyproline ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.4.481

    authors: Norris WE

    更新日期:1967-04-01 00:00:00

  • Fatty acid hydroperoxides and H2O2 in the execution of hypersensitive cell death in tobacco leaves.

    abstract::We initially compared lipid peroxidation profiles in tobacco (Nicotiana tabacum) leaves during different cell death events. An upstream oxylipin assay was used to discriminate reactive oxygen species (ROS)-mediated lipid peroxidation from 9- and 13-lipoxygenase (LOX)-dependent lipid peroxidation. Free radical-mediated...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.059907

    authors: Montillet JL,Chamnongpol S,Rustérucci C,Dat J,van de Cotte B,Agnel JP,Battesti C,Inzé D,Van Breusegem F,Triantaphylidès C

    更新日期:2005-07-01 00:00:00